FastQCFastQC Report
Thu 26 May 2016
SRR938313_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938313_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1602356
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126010.7864045193452641No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81570.5090629048725751No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69630.43454762861686164No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37940.23677634682929385No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA31250.19502532520863028No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30760.19196732810935896No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26190.16344682455084888No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26050.1625731110939142No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25630.1599519707231102No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22280.13904525586074507No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG21700.13542558582487288No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG19280.12032282464071654No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA19200.11982355980818245No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18010.11239699542423781No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17820.11121124144696934No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA16430.10253651498168946No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40650.048.6435471
GTATCAA77850.044.6788441
TATCAAC100400.034.457282
ATCAACG100400.034.457283
TCAACGC102350.033.893654
CAACGCA104600.033.028295
AACGCAG107450.032.190446
GTACATG90050.029.8423541
TACATGG90450.029.4739022
ACGCAGA122900.028.0628937
CGCAGAG123550.027.9152558
ACATGGG91450.027.9044763
GCAGAGT131100.025.6183349
CATGGGA54650.024.695144
CATGGGG36050.024.2546984
GTCTTAG6450.022.900221
ATGGGAT21700.022.5559355
GAGTACT84500.022.2891512-13
GAGTACA66650.022.2329941
GCACCGT3450.022.0344816
Produced by FastQC (version 0.11.5)