FastQCFastQC Report
Thu 26 May 2016
SRR938313_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938313_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1602356
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121270.756823078017619No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89300.5573043693161819No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77000.480542401314065No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42990.2682924393830085No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28420.17736383175773673No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26750.16694167837858753No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26130.1630723759264483No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24950.15570821964657042No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22340.13941970448514562No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG21250.1326172211418686No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA19360.12082208947325065No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18980.1184505815187137No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG18740.11695278702111142No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17060.10646822553789545No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51700.047.2789461
GTATCAA87900.039.0610161
TATCAAC113800.030.0456662
ATCAACG116300.029.2252853
TCAACGC117950.028.8164544
CAACGCA119700.028.395165
AACGCAG123750.027.5186276
GTACATG88400.026.8437711
TACATGG89100.026.5261332
TAAGGTG8600.025.4268055
ACATGGG90550.024.9891193
GGGTACG1351.3311905E-524.6580921
GTAAGGT8750.024.4476384
ACGCAGA139750.024.3000437
CGCAGAG141200.023.9832298
GGTAAGG8650.023.0815833
AGGTAAG8600.022.6715182
GCAGAGT148350.022.3470759
GAGTACT94900.021.69580312-13
AGTACTT96950.021.43300412-13
Produced by FastQC (version 0.11.5)