##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938313_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1602356 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56681037172763 31.0 31.0 34.0 30.0 34.0 2 31.804037305068288 31.0 31.0 34.0 30.0 34.0 3 31.85631407752085 31.0 31.0 34.0 30.0 34.0 4 35.58970540878556 37.0 35.0 37.0 33.0 37.0 5 35.46450039816371 37.0 35.0 37.0 33.0 37.0 6 35.52035253089825 37.0 35.0 37.0 33.0 37.0 7 35.442198238094406 37.0 35.0 37.0 33.0 37.0 8 35.42593343801253 37.0 35.0 37.0 33.0 37.0 9 36.93776351821942 39.0 37.0 39.0 33.0 39.0 10-11 36.86215173157525 39.0 37.0 39.0 32.5 39.0 12-13 36.735706047844545 39.0 36.0 39.0 32.0 39.0 14-15 37.65669863625811 39.0 36.5 41.0 32.0 41.0 16-17 37.573038076432454 39.0 36.0 41.0 32.0 41.0 18-19 37.60613902278894 39.0 36.5 41.0 32.0 41.0 20-21 37.60166560988944 39.0 36.5 41.0 32.0 41.0 22-23 37.60283544980017 39.0 36.5 41.0 32.0 41.0 24-25 37.487955547955636 39.0 36.0 41.0 32.0 41.0 26-27 37.26145219913677 39.0 36.0 41.0 31.0 41.0 28-29 37.09164349245735 39.0 36.0 40.5 31.0 41.0 30-31 36.75649699567387 39.0 35.5 40.0 30.5 41.0 32-33 36.44301172773092 38.0 35.0 40.0 30.0 41.0 34-35 36.19759341869098 38.0 35.0 40.0 30.0 41.0 36-37 36.128306381353454 38.0 35.0 40.0 30.0 41.0 38-39 35.925929069445246 38.0 35.0 40.0 29.5 41.0 40-41 35.682746842774016 38.0 35.0 40.0 28.5 41.0 42-43 35.370869207591824 38.0 34.0 40.0 27.0 41.0 44-45 34.98510786616707 38.0 34.0 40.0 26.0 41.0 46-47 34.47642346644565 38.0 33.0 40.0 24.5 41.0 48-49 33.95990528946127 37.5 33.0 40.0 23.0 41.0 50-51 33.51152646478061 37.0 32.5 40.0 22.5 41.0 52-53 32.912339704784706 36.5 31.5 39.5 19.5 41.0 54-55 32.527059529842305 36.0 31.0 39.0 18.0 40.0 56-57 32.1068673253634 36.0 31.0 39.0 15.0 40.0 58-59 31.586883314319664 35.0 30.0 39.0 12.0 40.0 60-61 31.112416029895975 35.0 29.0 38.0 8.0 40.0 62-63 30.56218093856796 34.5 28.5 38.0 7.5 40.0 64-65 30.00000998529665 34.0 28.0 38.0 4.0 40.0 66-67 29.44698057110904 34.0 27.5 37.0 2.0 39.0 68-69 28.81633138952892 33.5 26.0 36.5 2.0 39.0 70-71 28.18570436282574 33.0 26.0 36.0 2.0 39.0 72-73 27.53590587859377 32.5 25.0 35.5 2.0 38.0 74-75 26.86903409729174 32.0 23.5 35.0 2.0 37.5 76-77 25.72258006335671 30.5 22.5 34.0 2.0 36.0 78-79 26.29988997451253 31.5 23.5 35.0 2.0 36.0 80-81 25.959135797538124 32.0 22.0 35.0 2.0 36.0 82-83 25.389466198522676 31.0 20.0 34.5 2.0 36.0 84-85 24.71618604105455 31.0 18.0 34.0 2.0 35.0 86-87 23.97700542201608 30.5 10.5 34.0 2.0 35.0 88-89 23.112960228563438 30.0 4.5 34.0 2.0 35.0 90-91 22.469007823479927 29.5 2.0 34.0 2.0 35.0 92-93 21.58988795248996 29.0 2.0 34.0 2.0 35.0 94-95 20.68676030794655 28.5 2.0 34.0 2.0 35.0 96-97 19.611150393545508 26.5 2.0 33.0 2.0 35.0 98-99 18.381866139609425 25.0 2.0 33.0 2.0 35.0 100-101 15.608307704405263 10.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32311555780800205 1101 2 0.3628442198848312 1101 3 0.42839892486728104 1101 4 0.18778246305156898 1101 5 0.34826393661633404 1101 6 0.3781318277788088 1101 7 0.344206693063704 1101 8 0.3473360000901309 1101 9 0.4082188066567767 1101 10-11 0.44454663382560256 1101 12-13 0.3804687671245688 1101 14-15 0.5620274693764387 1101 16-17 0.5699558776920384 1101 18-19 0.5991825838331906 1101 20-21 0.7139419135276412 1101 22-23 0.6446324079156724 1101 24-25 0.6243406685675907 1101 26-27 0.5146086861338333 1101 28-29 0.5110482888890147 1101 30-31 0.5416471132769587 1101 32-33 0.5646915071303411 1101 34-35 0.4993865650085496 1101 36-37 0.601292047618017 1101 38-39 0.7659594961639371 1101 40-41 0.8215605397395578 1101 42-43 0.5528165329704251 1101 44-45 0.7638699545064256 1101 46-47 0.7560960676430568 1101 48-49 0.9140067122678275 1101 50-51 1.1591801529357895 1101 52-53 1.3944717110304836 1101 54-55 1.3284242720210742 1101 56-57 1.4423915295765184 1101 58-59 1.5467585870591165 1101 60-61 1.6239875805769124 1101 62-63 1.9127751884658046 1101 64-65 2.16149617368065 1101 66-67 2.025760379487025 1101 68-69 1.9974701010741356 1101 70-71 1.9009601980488817 1101 72-73 2.013524902997677 1101 74-75 2.1419637257181137 1101 76-77 2.158292798750118 1101 78-79 2.5079218174802875 1101 80-81 2.755401546530905 1101 82-83 2.944643533978301 1101 84-85 3.1447212022914606 1101 86-87 3.4052358246011067 1101 88-89 3.5817630032365884 1101 90-91 3.820861592013074 1101 92-93 4.151181458490736 1101 94-95 4.023946839725063 1101 96-97 4.096739536855761 1101 98-99 4.471018400099524 1101 100-101 4.795921228862895 1102 1 0.3043096809381751 1102 2 0.4215832300613691 1102 3 0.40711675754602084 1102 4 0.23737792488582699 1102 5 0.12669569799115266 1102 6 0.08589263471607467 1102 7 0.18688650672913099 1102 8 0.23223797423333536 1102 9 0.37566734826785364 1102 10-11 0.23395708562872386 1102 12-13 0.36093933843363146 1102 14-15 0.6172640472237205 1102 16-17 0.4618792733081776 1102 18-19 0.6175576029115959 1102 20-21 0.6348796991089003 1102 22-23 0.6795433075140025 1102 24-25 0.6432082052980235 1102 26-27 0.729418699542947 1102 28-29 0.5711607534298366 1102 30-31 0.6967060574393855 1102 32-33 0.5427474478485692 1102 34-35 0.6795346019603201 1102 36-37 0.8073435061972489 1102 38-39 1.0036034500530278 1102 40-41 0.8740795163228867 1102 42-43 0.9264983155646078 1102 44-45 0.8000559191235652 1102 46-47 0.900984370044327 1102 48-49 1.0300218333970932 1102 50-51 1.1999445525114751 1102 52-53 1.282654408436244 1102 54-55 1.134206178049162 1102 56-57 1.3403808091343379 1102 58-59 1.364839161272286 1102 60-61 1.4654633141865254 1102 62-63 1.6332257469633156 1102 64-65 1.5433387530115787 1102 66-67 1.5382156713303736 1102 68-69 1.5798069488926494 1102 70-71 1.8309880553649158 1102 72-73 1.7556978117102524 1102 74-75 1.9337859534888793 1102 76-77 2.0990565132162438 1102 78-79 2.530788110356255 1102 80-81 2.829983715336571 1102 82-83 2.763970817286708 1102 84-85 2.8808421195618195 1102 86-87 2.956944088202338 1102 88-89 3.0940125719030256 1102 90-91 3.394077157550086 1102 92-93 3.6106315362697003 1102 94-95 3.681095368052631 1102 96-97 4.038152963556836 1102 98-99 4.12942010800154 1102 100-101 4.2297038671969815 1103 1 0.2758704850228213 1103 2 0.34551390023895223 1103 3 0.3393202834443514 1103 4 0.2618753719812972 1103 5 0.2179580209737111 1103 6 0.24338597147468732 1103 7 0.2997240841993474 1103 8 0.19064489758197567 1103 9 0.34561452401686665 1103 10-11 0.2798190521680084 1103 12-13 0.4282059644250822 1103 14-15 0.5800823574899354 1103 16-17 0.5766014241703772 1103 18-19 0.5652752420368614 1103 20-21 0.5427443309447497 1103 22-23 0.5684779114124083 1103 24-25 0.5404216859184245 1103 26-27 0.5077153984286653 1103 28-29 0.566249623408325 1103 30-31 0.45443903004784403 1103 32-33 0.5748975875358013 1103 34-35 0.6277279001845173 1103 36-37 0.9246085265883721 1103 38-39 1.0088055951350654 1103 40-41 0.8581338980061943 1103 42-43 1.062494670851855 1103 44-45 1.2879978606154907 1103 46-47 1.3285818299845928 1103 48-49 1.3045032244892312 1103 50-51 1.2056163356912108 1103 52-53 1.3719599060117815 1103 54-55 1.4731142895420533 1103 56-57 1.4054598046707625 1103 58-59 1.7444351922282522 1103 60-61 1.641611719825395 1103 62-63 1.813434314446699 1103 64-65 1.6477810477355739 1103 66-67 1.833850846822493 1103 68-69 1.8313443112124226 1103 70-71 1.767749479211293 1103 72-73 2.188973075831509 1103 74-75 2.3503629741368997 1103 76-77 2.0893867852049404 1103 78-79 2.713812499425323 1103 80-81 2.9958582858384837 1103 82-83 3.337968922678318 1103 84-85 3.222200778564421 1103 86-87 3.5476424440675984 1103 88-89 3.8303810250676626 1103 90-91 3.903273233462869 1103 92-93 4.013466625059401 1103 94-95 4.134145607287081 1103 96-97 4.436500399413106 1103 98-99 4.694890468619377 1103 100-101 4.673104448424301 1104 1 0.35954894038077256 1104 2 0.3871401302651343 1104 3 0.27832396223825384 1104 4 0.29828245422378075 1104 5 0.12638595723804258 1104 6 0.22800500189809014 1104 7 0.2616948344024692 1104 8 0.2763348407073707 1104 9 0.3711262826157693 1104 10-11 0.47813820446555866 1104 12-13 0.4392211874096503 1104 14-15 0.6588491716092761 1104 16-17 0.6619485962386307 1104 18-19 0.5418229309417981 1104 20-21 0.5001264628396669 1104 22-23 0.4623303195554058 1104 24-25 0.5173065035585793 1104 26-27 0.6607506855100027 1104 28-29 0.7273294509789636 1104 30-31 0.7338341167139149 1104 32-33 0.9031862148717238 1104 34-35 0.8573436016379858 1104 36-37 0.8798282386042544 1104 38-39 0.8226837322660572 1104 40-41 0.7571165873625247 1104 42-43 0.8332753425138506 1104 44-45 0.8650183760980212 1104 46-47 0.9260120344623459 1104 48-49 1.1584108145406802 1104 50-51 1.2225743342467794 1104 52-53 1.4735879980042057 1104 54-55 1.4725119716762052 1104 56-57 1.6096201707446127 1104 58-59 1.90155693096618 1104 60-61 1.7186731421419061 1104 62-63 1.853094195280864 1104 64-65 1.756992420399655 1104 66-67 1.9029636481224834 1104 68-69 1.9196621657759323 1104 70-71 2.0590667921118992 1104 72-73 2.2295018742426294 1104 74-75 2.4589692077741674 1104 76-77 2.2924694677904753 1104 78-79 2.6932998380549193 1104 80-81 2.960317300011994 1104 82-83 3.313486887831335 1104 84-85 3.296675196276798 1104 86-87 3.677800936890563 1104 88-89 3.820505551112621 1104 90-91 4.09619127309454 1104 92-93 4.32095342202609 1104 94-95 4.641161834604947 1104 96-97 4.691117229330953 1104 98-99 5.139489920185685 1104 100-101 4.828034145093078 1105 1 0.23703903389139924 1105 2 0.25590574300291635 1105 3 0.21425336226698732 1105 4 0.03777667060251133 1105 5 0.11381044961682107 1105 6 0.15470665215065083 1105 7 0.15530853381996224 1105 8 0.25309742208703767 1105 9 0.24203070226405288 1105 10-11 0.28298554504983997 1105 12-13 0.3681191560957444 1105 14-15 0.4638598932769966 1105 16-17 0.4505080862962245 1105 18-19 0.4117864413484469 1105 20-21 0.3141074277344913 1105 22-23 0.17312248638234706 1105 24-25 0.13618869370791487 1105 26-27 0.339930167056572 1105 28-29 0.4506520488279975 1105 30-31 0.48255591016152977 1105 32-33 0.4622294787352814 1105 34-35 0.4774395785256047 1105 36-37 0.5435999859044998 1105 38-39 0.48079119902336487 1105 40-41 0.6438962598542659 1105 42-43 0.7007743200867793 1105 44-45 0.8425999353822107 1105 46-47 0.8096513705479467 1105 48-49 0.698382704704386 1105 50-51 0.8183049412921193 1105 52-53 0.963827791694996 1105 54-55 0.8673548287795825 1105 56-57 0.9008576598295122 1105 58-59 1.1111786605811957 1105 60-61 1.3245439115044668 1105 62-63 1.3525187346360141 1105 64-65 1.5792101407188746 1105 66-67 1.5875497309556543 1105 68-69 1.5829809018902665 1105 70-71 1.5753211374269718 1105 72-73 1.6967545021565478 1105 74-75 1.899544433537116 1105 76-77 1.7880659503650023 1105 78-79 2.025198664750768 1105 80-81 2.467745785382995 1105 82-83 2.508681342407943 1105 84-85 2.722574275816097 1105 86-87 2.880468262608197 1105 88-89 3.253669734939482 1105 90-91 3.2505958958465797 1105 92-93 3.6620195509513813 1105 94-95 3.491155548060089 1105 96-97 3.5640891277846016 1105 98-99 3.9621093576297284 1105 100-101 4.324347535571789 1106 1 0.1504031531424701 1106 2 0.23617762428899525 1106 3 0.13387822836826757 1106 4 0.051446681328215504 1106 5 0.05120160027070142 1106 6 0.05609091377365161 1106 7 0.17230706908965487 1106 8 0.17280099629825685 1106 9 0.19946687533766294 1106 10-11 0.1485391478617899 1106 12-13 0.2122499993530269 1106 14-15 0.01665456625688222 1106 16-17 0.14561340844885962 1106 18-19 0.37262207982828954 1106 20-21 0.3892873085607462 1106 22-23 0.39099171285767653 1106 24-25 0.2883694434302768 1106 26-27 0.12838658710104767 1106 28-29 0.5597991394414024 1106 30-31 0.4695478415446317 1106 32-33 0.45366496621982577 1106 34-35 0.4236104807587395 1106 36-37 0.3815991912287586 1106 38-39 0.5311473014391837 1106 40-41 0.3711805987700032 1106 42-43 0.6675722704999814 1106 44-45 0.2001140916226447 1106 46-47 0.15057261445367232 1106 48-49 0.6556911497048432 1106 50-51 0.6378747117164494 1106 52-53 0.8292873245071348 1106 54-55 0.9076915268846122 1106 56-57 0.9265239257061992 1106 58-59 1.4472431308083031 1106 60-61 1.2373543122978994 1106 62-63 1.416817219263251 1106 64-65 1.1021751304540253 1106 66-67 1.2937649789949468 1106 68-69 1.2604392098937431 1106 70-71 1.4743850632563174 1106 72-73 1.7782831586401784 1106 74-75 2.2010039166388644 1106 76-77 2.215005665130956 1106 78-79 2.525385941722881 1106 80-81 2.657493087472666 1106 82-83 3.0549931252536453 1106 84-85 3.0002702961200285 1106 86-87 3.2446008043036443 1106 88-89 3.6084152988917246 1106 90-91 3.5664097704983924 1106 92-93 3.271518584187948 1106 94-95 3.635667607834062 1106 96-97 4.241026857587254 1106 98-99 4.466227552355662 1106 100-101 4.523122105533808 1107 1 0.12588800856532245 1107 2 0.14991688076496246 1107 3 0.20776419287049208 1107 4 0.22689304790242915 1107 5 0.1453648414412072 1107 6 0.1186335867491195 1107 7 0.19138668743586607 1107 8 0.14627985933493903 1107 9 0.26951282651892683 1107 10-11 0.14593347328159467 1107 12-13 0.31514863958431505 1107 14-15 0.37284361065179183 1107 16-17 0.3733873779082302 1107 18-19 0.31098959305627005 1107 20-21 0.4081082873035129 1107 22-23 0.29880392549218726 1107 24-25 0.37504134885689666 1107 26-27 0.33702276136408926 1107 28-29 0.4831099379178099 1107 30-31 0.4835109679992726 1107 32-33 0.5473875305461888 1107 34-35 0.5890180138589827 1107 36-37 0.7036061799574611 1107 38-39 0.5355632826369714 1107 40-41 0.7580230425525727 1107 42-43 0.7755790428995155 1107 44-45 0.7861997927765216 1107 46-47 0.8935617921946957 1107 48-49 0.6705281231574389 1107 50-51 0.7102448365902418 1107 52-53 0.9974875170196142 1107 54-55 0.9012849078945351 1107 56-57 0.7928024737348593 1107 58-59 0.7127404371007202 1107 60-61 0.9142494701072934 1107 62-63 1.0452060207826044 1107 64-65 1.251507086195943 1107 66-67 1.4544318665402862 1107 68-69 1.4617918472499696 1107 70-71 1.5495985177021367 1107 72-73 1.7513530776600525 1107 74-75 1.6357033879972604 1107 76-77 1.8665063719540953 1107 78-79 1.971269528651547 1107 80-81 2.184304938079972 1107 82-83 2.3429707512218805 1107 84-85 2.6580112804996254 1107 86-87 2.9054446108917205 1107 88-89 3.2134228072881257 1107 90-91 3.072325895517544 1107 92-93 3.2676132285397266 1107 94-95 3.310510538869078 1107 96-97 3.8527245331405915 1107 98-99 4.02639589329862 1107 100-101 3.902725711338391 1108 1 0.11156179008799327 1108 2 0.16296289105454775 1108 3 0.18897105614412624 1108 4 0.17199883895594326 1108 5 0.014394252618657788 1108 6 0.04726871909608832 1108 7 0.21950767326605813 1108 8 0.15468823476505378 1108 9 0.21913512017811598 1108 10-11 0.18991845820661268 1108 12-13 0.2852070093571655 1108 14-15 0.46868434836052586 1108 16-17 0.5534912388324287 1108 18-19 0.3071165223536383 1108 20-21 0.29861038715109345 1108 22-23 0.3705423397245653 1108 24-25 0.3731844313399719 1108 26-27 0.33657683493529333 1108 28-29 0.2947700820785073 1108 30-31 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2316 96-97 3.6243723749868444 2316 98-99 4.014110932731047 2316 100-101 4.189064414207291 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 14.0 4 38.0 5 125.0 6 250.0 7 618.0 8 1191.0 9 2035.0 10 3277.0 11 5039.0 12 7142.0 13 9995.0 14 12777.0 15 16604.0 16 20592.0 17 25464.0 18 23697.0 19 20283.0 20 20572.0 21 21821.0 22 23768.0 23 26637.0 24 30515.0 25 34821.0 26 40608.0 27 47125.0 28 55197.0 29 63960.0 30 74329.0 31 84841.0 32 98823.0 33 114608.0 34 137108.0 35 164220.0 36 177519.0 37 165107.0 38 69103.0 39 2530.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.341893650283495 19.794365168767218 14.575609025465644 22.28813215548365 2 16.355088840885536 21.703397555158766 38.48421092062382 23.457302683331875 3 19.591687973873423 25.77833972913584 30.01473458095688 24.615237716033857 4 13.275888753810014 17.1854444330723 35.880915352143965 33.657751460973714 5 13.063167179753718 38.55493113305613 33.64291390132736 14.738987785862786 6 32.004248743724865 36.54680982253631 16.688987965221212 14.759953468517608 7 28.29071691933628 33.87468203070978 20.15463480025662 17.679966249697323 8 27.272965558215528 34.79108263082611 19.246971334709638 18.688980476248723 9 27.29830324846663 16.12288405323162 19.674966112399492 36.90384658590226 10-11 25.2198637506272 27.08692699999251 26.64067785186313 21.052531397517154 12-13 26.326297027626822 24.456331801422408 27.626351447493562 21.591019723457208 14-15 22.588582062912362 25.603860814949986 26.45626190434585 25.3512952177918 16-17 21.441209069644948 29.0050088744324 27.33765155808073 22.216130497841927 18-19 21.606060076537297 26.52565971606809 30.753122277446458 21.11515792994815 20-21 22.052028388198377 25.8307454772847 31.028591648797143 21.08863448571978 22-23 21.76807151469461 25.959930252702897 31.0444433072301 21.227554925372388 24-25 21.558158692911615 26.254883482906248 31.10965837462711 21.077299449555024 26-27 21.04480667493877 26.635592183610747 30.883357092746593 21.436244048703884 28-29 21.187925779289994 26.910998554628314 30.841304928492796 21.059770737588902 30-31 21.219223443479475 26.553899383158296 30.69683016757824 21.530047005783985 32-33 20.87579059835061 26.631599345085473 30.65582257021187 21.836787486352048 34-35 21.65503776415481 26.766251725454083 30.43106469606261 21.1476458143285 36-37 21.89064056840667 26.314708792685032 30.405163566078464 21.389487072829834 38-39 21.31671782683106 26.581429546898622 30.523300448520057 21.57855217775026 40-41 21.65206629210818 26.529138544614362 30.515427756855708 21.303367406421753 42-43 21.458700586075306 26.680640604243294 30.13012488231529 21.730533927366107 44-45 21.340290374612394 26.940113629732583 30.297548632146288 21.422047363508735 46-47 21.933858643147968 27.250966701531993 29.346412407729616 21.468762247590423 48-49 21.67932719071168 27.415755300320278 29.0936283822072 21.811289126760844 50-51 21.880686938483084 27.662891392424655 28.92035228126584 21.536069387826426 52-53 22.233167910252153 27.32395297923807 28.895170611274896 21.547708499234876 54-55 21.431286181098333 27.60996932017604 29.13007471498219 21.828669783743436 56-57 21.708627795571022 27.883098387624223 28.66912845834509 21.73914535845967 58-59 21.605685627912898 27.373473809815046 28.85416848690304 22.166672075369018 60-61 21.26503099186448 27.622045288312957 29.152666448654358 21.960257271168203 62-63 21.53054627061652 27.587596014868105 29.190204923250512 21.69165279126486 64-65 21.125954531951702 27.60822189326217 29.10632843138479 22.159495143401344 66-67 21.033950135738134 27.774581084032828 29.002090679314758 22.189378100914283 68-69 21.646498911131545 27.276474606227158 29.099576466212312 21.97745001642898 70-71 21.408756855530232 27.558482634320963 28.682483792615376 22.350276717533433 72-73 21.371800060473507 27.660639390347143 28.648030133157178 22.319530416022175 74-75 21.333835926598084 28.10224444505466 28.24032237530237 22.32359725304489 76-77 21.337424392581923 27.628254894667602 28.808579366882263 22.225741345868208 78-79 21.46511137350252 27.743335438566714 28.694310128336024 22.097243059594746 80-81 21.478018753028746 27.964941590640525 28.45678031921151 22.100259337119223 82-83 21.441434357183223 27.983451645084095 28.376596771772682 22.19851722596 84-85 21.322695582110754 27.945597557482117 28.368931541970476 22.362775318436654 86-87 21.474789837299433 27.882614692354256 28.22059126397184 22.422004206374467 88-89 21.243014188491312 28.011426930985955 27.913196513881132 22.832362366641597 90-91 21.212483371969547 27.96592107977117 28.1892507798753 22.632344768383977 92-93 21.370872745461906 27.591636416853603 28.332173059406752 22.70531777827774 94-95 21.11422180838715 27.405021106420797 28.423739793154578 23.057017292037475 96-97 21.355179498188917 26.982112589212388 28.371847454623065 23.290860457975633 98-99 21.008408867941956 27.349009833020876 28.06957380257571 23.57300749646146 100-101 21.10208716431547 26.981670261926062 28.51414632898168 23.402096244776786 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3288.0 1 2182.5 2 2044.5 3 2757.5 4 1956.0 5 1618.5 6 2678.5 7 5157.5 8 7167.0 9 6941.0 10 5069.0 11 3418.5 12 3044.0 13 3088.5 14 3144.0 15 3147.5 16 3192.5 17 3297.0 18 3467.5 19 3644.5 20 3982.5 21 4647.0 22 5465.5 23 6388.5 24 7482.0 25 9023.0 26 10889.5 27 13086.0 28 15241.0 29 17303.5 30 19738.5 31 23401.5 32 29195.0 33 31102.5 34 29785.5 35 30898.0 36 33800.5 37 37146.0 38 39685.0 39 41404.5 40 43245.0 41 46213.5 42 48659.5 43 50417.5 44 51847.0 45 54679.5 46 56637.5 47 57197.0 48 58320.5 49 57115.0 50 55530.5 51 53823.5 52 51652.5 53 51577.0 54 52116.0 55 48983.0 56 44143.0 57 40271.5 58 36925.5 59 32593.0 60 26731.5 61 22610.0 62 19744.5 63 15230.5 64 10798.0 65 8293.5 66 6561.0 67 5417.5 68 4222.5 69 3063.0 70 2289.5 71 1648.0 72 1273.5 73 1076.5 74 872.0 75 674.0 76 503.5 77 351.5 78 242.5 79 166.0 80 115.5 81 76.5 82 45.5 83 30.5 84 19.5 85 9.5 86 5.5 87 3.5 88 3.0 89 2.0 90 0.5 91 0.5 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.24081040667617E-5 2 0.0384433921051252 3 1.872243122002851E-4 4 0.0 5 0.09910406925801755 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.98529665068187E-4 26-27 0.011483091148284151 28-29 0.0 30-31 0.0 32-33 5.928769886342361E-4 34-35 0.015071557132122949 36-37 0.003276425463504989 38-39 0.012356804605218817 40-41 0.007114523863610833 42-43 0.005460709105841648 44-45 0.002901976839104419 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.744486244005702E-4 68-69 2.8083646830042764E-4 70-71 0.0 72-73 9.361215610014255E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.361215610014255E-5 82-83 0.0013417742374353763 84-85 0.004680607805007127 86-87 0.0016226107057358042 88-89 6.24081040667617E-5 90-91 0.001029733717101568 92-93 2.8083646830042764E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 9.049175089680445E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1602356.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.39089579223224 #Duplication Level Percentage of deduplicated Percentage of total 1 75.85583317809964 39.74155051266771 2 13.37346723809584 14.01295856903822 3 4.540499589818475 7.136425225645588 4 2.0389974104769704 4.272996034117213 5 1.1090401097142275 2.905180240872195 6 0.6847591689328117 2.152508775740069 7 0.4490958800226684 1.6469974815691915 8 0.30947551884368607 1.297095972638925 9 0.23742244831662526 1.119489727364366 >10 1.2651843229993616 12.122998259673974 >50 0.08487945715958667 3.0107268214454685 >100 0.04382312965879587 4.379172252603623 >500 0.003940382213016032 1.4559035460001635 >1k 0.0032239490833767532 2.947027033408744 >5k 2.3881104321309281E-4 1.0403332604297992 >10k+ 1.1940552160654641E-4 0.7586362867848573 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12127 0.756823078017619 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8930 0.5573043693161819 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7700 0.480542401314065 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4299 0.2682924393830085 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2842 0.17736383175773673 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2675 0.16694167837858753 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2613 0.1630723759264483 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2495 0.15570821964657042 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2234 0.13941970448514562 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2125 0.1326172211418686 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1936 0.12082208947325065 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1898 0.1184505815187137 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 1874 0.11695278702111142 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1706 0.10646822553789545 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 6.24081040667617E-5 10-11 0.0 0.0 0.0 0.0 6.24081040667617E-5 12-13 0.0 0.0 0.0 0.0 6.24081040667617E-5 14-15 0.0 0.0 0.0 0.0 9.361215610014255E-5 16-17 0.0 0.0 0.0 0.0 1.248162081335234E-4 18-19 0.0 0.0 0.0 1.248162081335234E-4 1.248162081335234E-4 20-21 0.0 0.0 0.0 2.184283642336659E-4 1.248162081335234E-4 22-23 0.0 0.0 0.0 5.304688845674744E-4 1.248162081335234E-4 24-25 0.0 0.0 0.0 0.001372978289468757 1.248162081335234E-4 26-27 0.0 0.0 0.0 0.0023403039025035634 1.248162081335234E-4 28-29 0.0 0.0 0.0 0.004930240221274173 1.248162081335234E-4 30-31 0.0 0.0 0.0 0.015726842224823945 1.248162081335234E-4 32-33 0.0 0.0 0.0 0.035510211213987404 1.248162081335234E-4 34-35 0.0 0.0 0.0 0.06306338915946269 1.248162081335234E-4 36-37 0.0 0.0 0.0 0.11089920092663552 1.248162081335234E-4 38-39 0.0 0.0 0.0 0.18479039614168136 1.248162081335234E-4 40-41 0.0 0.0 0.0 0.27166247700261364 1.248162081335234E-4 42-43 0.0 0.0 0.0 0.3647441642181887 1.248162081335234E-4 44-45 0.0 0.0 0.0 0.4706819208715167 1.248162081335234E-4 46-47 0.0 0.0 0.0 0.5957477614213071 1.248162081335234E-4 48-49 0.0 0.0 0.0 0.7368836887682887 1.248162081335234E-4 50-51 0.0 0.0 0.0 0.8910941139172568 1.248162081335234E-4 52-53 0.0 0.0 0.0 1.0363177720806114 1.248162081335234E-4 54-55 0.0 0.0 0.0 1.19754910893709 1.248162081335234E-4 56-57 0.0 0.0 0.0 1.387800214184613 1.5602026016690423E-4 58-59 0.0 0.0 0.0 1.5875997593543507 2.184283642336659E-4 60-61 0.0 0.0 0.0 1.8169807458517333 2.496324162670468E-4 62-63 0.0 0.0 0.0 2.039247208485505 2.8083646830042764E-4 64-65 0.0 0.0 0.0 2.264727688478715 3.1204052033380846E-4 66-67 0.0 0.0 0.0 2.5303053753348195 3.1204052033380846E-4 68-69 0.0 0.0 0.0 2.82196964969083 3.1204052033380846E-4 70-71 0.0 0.0 0.0 3.13937726697438 3.1204052033380846E-4 72-73 0.0 0.0 0.0 3.464305060797975 4.05652676433951E-4 74-75 0.0 0.0 0.0 3.7850515116490966 4.368567284673319E-4 76-77 0.0 0.0 0.0 4.134474486318895 4.368567284673319E-4 78-79 0.0 0.0 0.0 4.5345104333868385 4.368567284673319E-4 80-81 0.0 0.0 0.0 4.960040090966052 4.368567284673319E-4 82-83 0.0 0.0 0.0 5.40507228106613 4.992648325340936E-4 84-85 6.24081040667617E-5 0.0 0.0 5.844799782320533 4.992648325340936E-4 86-87 6.24081040667617E-5 0.0 0.0 6.29388849918495 4.992648325340936E-4 88-89 6.24081040667617E-5 0.0 0.0 6.758267201545724 4.992648325340936E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5170 0.0 47.278946 1 GTATCAA 8790 0.0 39.061016 1 TATCAAC 11380 0.0 30.045666 2 ATCAACG 11630 0.0 29.225285 3 TCAACGC 11795 0.0 28.816454 4 CAACGCA 11970 0.0 28.39516 5 AACGCAG 12375 0.0 27.518627 6 GTACATG 8840 0.0 26.843771 1 TACATGG 8910 0.0 26.526133 2 TAAGGTG 860 0.0 25.426805 5 ACATGGG 9055 0.0 24.989119 3 GGGTACG 135 1.3311905E-5 24.658092 1 GTAAGGT 875 0.0 24.447638 4 ACGCAGA 13975 0.0 24.300043 7 CGCAGAG 14120 0.0 23.983229 8 GGTAAGG 865 0.0 23.081583 3 AGGTAAG 860 0.0 22.671518 2 GCAGAGT 14835 0.0 22.347075 9 GAGTACT 9490 0.0 21.695803 12-13 AGTACTT 9695 0.0 21.433004 12-13 >>END_MODULE