##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938312_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1591533 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.58980115398173 31.0 31.0 34.0 30.0 34.0 2 31.748855977224476 33.0 31.0 34.0 30.0 34.0 3 31.810177985627693 33.0 31.0 34.0 30.0 34.0 4 35.50091138543782 37.0 35.0 37.0 33.0 37.0 5 35.51114742829712 37.0 35.0 37.0 33.0 37.0 6 35.514703747895894 37.0 35.0 37.0 33.0 37.0 7 35.45069879166816 37.0 35.0 37.0 33.0 37.0 8 35.42054861570574 37.0 35.0 37.0 33.0 37.0 9 36.98005319399598 39.0 37.0 39.0 33.0 39.0 10-11 36.84027035568851 39.0 37.0 39.0 33.0 39.0 12-13 36.68156519531797 39.0 37.0 39.0 32.0 39.0 14-15 37.900387865033274 40.0 37.5 41.0 33.0 41.0 16-17 37.85249284809049 40.0 37.5 41.0 33.0 41.0 18-19 37.883022532363455 40.0 37.5 41.0 33.0 41.0 20-21 37.83545455859225 40.0 37.5 41.0 33.0 41.0 22-23 37.69435883516082 40.0 37.0 41.0 32.5 41.0 24-25 37.56234146574404 40.0 37.0 41.0 32.0 41.0 26-27 37.35440327030605 39.5 36.5 41.0 32.0 41.0 28-29 37.13312730556011 39.0 36.0 41.0 31.0 41.0 30-31 36.834611660581345 39.0 36.0 40.5 31.0 41.0 32-33 36.502914171430945 39.0 35.0 40.0 30.0 41.0 34-35 36.205516002495706 38.0 35.0 40.0 30.0 41.0 36-37 35.96414117709152 38.0 35.0 40.0 29.5 41.0 38-39 35.72228097061135 38.0 35.0 40.0 28.5 41.0 40-41 35.441013475686646 38.0 35.0 40.0 27.5 41.0 42-43 35.011703181775054 38.0 34.0 40.0 26.0 41.0 44-45 34.532740760009375 38.0 33.5 40.0 24.5 41.0 46-47 33.99867800416328 38.0 33.0 40.0 23.0 41.0 48-49 33.4826679685561 37.5 33.0 40.0 21.0 41.0 50-51 32.7254690917499 36.5 31.5 39.5 19.0 40.5 52-53 32.75053203420853 37.0 32.0 39.0 18.5 40.0 54-55 32.78586463491489 37.0 32.0 39.5 16.5 40.5 56-57 32.402685963784606 36.0 31.0 39.0 13.0 41.0 58-59 31.99668652802047 36.0 31.0 39.0 8.5 40.0 60-61 31.492984122855134 35.5 30.5 39.0 6.5 40.0 62-63 30.82607272359417 35.0 29.5 38.5 2.0 40.0 64-65 30.216191558704722 35.0 28.5 38.0 2.0 40.0 66-67 29.581382855397912 34.0 28.0 37.5 2.0 40.0 68-69 28.787926483459657 34.0 26.5 37.0 2.0 39.0 70-71 27.993200266661137 33.5 26.0 36.0 2.0 39.0 72-73 27.14833402763248 33.0 24.0 35.5 2.0 38.5 74-75 26.276264771135757 32.0 21.5 35.0 2.0 38.0 76-77 25.373770760643982 31.5 19.0 35.0 2.0 37.0 78-79 24.431773956305022 31.0 12.5 34.5 2.0 36.0 80-81 23.514338376898248 30.0 6.0 34.0 2.0 36.0 82-83 22.539932882321636 29.0 2.0 34.0 2.0 35.0 84-85 21.59031795130858 29.0 2.0 34.0 2.0 35.0 86-87 20.6208064802929 27.5 2.0 33.5 2.0 35.0 88-89 19.528732674723052 25.5 2.0 33.0 2.0 35.0 90-91 18.35393234070547 24.5 2.0 32.0 2.0 34.0 92-93 17.177662982797088 20.0 2.0 32.0 2.0 34.0 94-95 15.862632128897108 8.5 2.0 31.0 2.0 34.0 96-97 14.350380105219308 2.0 2.0 31.0 2.0 34.0 98-99 12.55114816972064 2.0 2.0 29.5 2.0 34.0 100-101 8.795215996149624 2.0 2.0 13.5 2.0 29.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4391548531753564 1101 2 0.28435616696311783 1101 3 0.2870342812414499 1101 4 0.10948828952392375 1101 5 0.19580366425964968 1101 6 0.3596798281828981 1101 7 0.20277659783092616 1101 8 0.26231897724800035 1101 9 0.2614854516063829 1101 10-11 0.35981566663630105 1101 12-13 0.38134855123625044 1101 14-15 0.4557695242368922 1101 16-17 0.42716826827101073 1101 18-19 0.35231444989261007 1101 20-21 0.48985796986437435 1101 22-23 0.4044925672084858 1101 24-25 0.5501165778965671 1101 26-27 0.633870777258899 1101 28-29 0.6114676305686331 1101 30-31 0.5925806162202818 1101 32-33 0.7775827537982138 1101 34-35 0.6274541175514017 1101 36-37 0.7298336460875277 1101 38-39 0.9150870190043534 1101 40-41 0.7761676889718316 1101 42-43 1.054995949160059 1101 44-45 1.1381104261487636 1101 46-47 1.1112091659074395 1101 48-49 0.8602456068184949 1101 50-51 1.0039690401213406 1101 52-53 0.8439270012857634 1101 54-55 0.9282635920639137 1101 56-57 1.2517384316443625 1101 58-59 1.2323287343311229 1101 60-61 1.1990028189235957 1101 62-63 1.292639816744849 1101 64-65 1.282703793742936 1101 66-67 1.320243720176773 1101 68-69 1.6102481388062628 1101 70-71 1.9084671193378462 1101 72-73 2.1559107626430034 1101 74-75 2.0771856146943115 1101 76-77 2.3965197696528833 1101 78-79 2.364874708531101 1101 80-81 2.849569263247343 1101 82-83 2.862920915938602 1101 84-85 3.1395755271604564 1101 86-87 3.1714012649454695 1101 88-89 3.2432337144583308 1101 90-91 3.441753913809386 1101 92-93 3.4576208581798618 1101 94-95 3.571626096375045 1101 96-97 3.485762250375309 1101 98-99 3.6265926171573355 1101 100-101 2.685046115310362 1102 1 0.4476177449817875 1102 2 0.3880309843550158 1102 3 0.32217601234154003 1102 4 0.15464626823977312 1102 5 0.26506015452042675 1102 6 0.354596560086776 1102 7 0.4590201161687446 1102 8 0.39884642883974664 1102 9 0.4249829733402848 1102 10-11 0.43248038029917524 1102 12-13 0.4535162284253573 1102 14-15 0.44952226737726164 1102 16-17 0.47001527973407065 1102 18-19 0.6645240134799977 1102 20-21 0.5716676686367208 1102 22-23 0.4536842240241441 1102 24-25 0.5208000245444993 1102 26-27 0.5902769960656045 1102 28-29 0.47554170713505783 1102 30-31 0.47474128741952626 1102 32-33 0.561907991736426 1102 34-35 0.4335601704425116 1102 36-37 0.44814761761644206 1102 38-39 0.6797452022173402 1102 40-41 0.5912833924831276 1102 42-43 0.8406990193089285 1102 44-45 0.7296795360889661 1102 46-47 0.5671334892536848 1102 48-49 0.7675951294466472 1102 50-51 0.762406323053419 1102 52-53 0.8402153968393122 1102 54-55 0.7059989376192206 1102 56-57 0.8914038115118288 1102 58-59 1.23953660436813 1102 60-61 1.0478839167330491 1102 62-63 1.0084041164038204 1102 64-65 1.2054992061280565 1102 66-67 1.6588007030999492 1102 68-69 1.6643155757579642 1102 70-71 1.3468652524696019 1102 72-73 1.5811844411316223 1102 74-75 1.5560599756874716 1102 76-77 1.885301400351235 1102 78-79 2.1560827520355303 1102 80-81 2.2795362820079923 1102 82-83 2.5042366662561903 1102 84-85 2.6742718133876693 1102 86-87 2.777053573445297 1102 88-89 2.9774861259918204 1102 90-91 2.8734546720563756 1102 92-93 2.9695116006278646 1102 94-95 2.7989626164243617 1102 96-97 2.6837465141893375 1102 98-99 2.334230678898276 1102 100-101 1.8943472584755607 1103 1 0.3806089360182696 1103 2 0.386486177685061 1103 3 0.4271173562578561 1103 4 0.27916583554837615 1103 5 0.30008207869610715 1103 6 0.24589106878295297 1103 7 0.2644821683530907 1103 8 0.3312627742477474 1103 9 0.36951910202420635 1103 10-11 0.43742186974602504 1103 12-13 0.37890272115392776 1103 14-15 0.5802647205273459 1103 16-17 0.5842902247599113 1103 18-19 0.5472449543058246 1103 20-21 0.5783791732624124 1103 22-23 0.629870043237382 1103 24-25 0.7138921264566278 1103 26-27 0.7708436483247496 1103 28-29 0.5829694403077212 1103 30-31 0.7107645344678062 1103 32-33 0.8020301064115856 1103 34-35 0.8155443629153822 1103 36-37 0.923745784513784 1103 38-39 0.8695512245621089 1103 40-41 0.9775596443732084 1103 42-43 1.2134194653309294 1103 44-45 1.1586180654446636 1103 46-47 1.3288708910387683 1103 48-49 1.1478777060939436 1103 50-51 1.2360205448797998 1103 52-53 1.2004726075136816 1103 54-55 1.4344462286410007 1103 56-57 1.5383576390377627 1103 58-59 1.6800393541676648 1103 60-61 1.606157255101735 1103 62-63 1.8682043611861836 1103 64-65 1.9715297588093854 1103 66-67 2.0830607894577717 1103 68-69 2.1823948346000357 1103 70-71 2.221557273466747 1103 72-73 2.3730648557772334 1103 74-75 2.2506455847546043 1103 76-77 2.440392713487835 1103 78-79 2.812722728401372 1103 80-81 2.9118897582232073 1103 82-83 2.928938073973093 1103 84-85 2.9089394434665046 1103 86-87 3.082322797665654 1103 88-89 3.21304840505827 1103 90-91 3.0845623631998222 1103 92-93 3.053743221212283 1103 94-95 3.079608801122994 1103 96-97 3.409888601475643 1103 98-99 3.372421166731195 1103 100-101 2.5509683158713496 1104 1 0.49671691191534606 1104 2 0.47810474640330014 1104 3 0.42065820954985256 1104 4 0.35095491359300723 1104 5 0.2670903253404049 1104 6 0.41217769091630885 1104 7 0.420764670269989 1104 8 0.4150004800256468 1104 9 0.5878363973402045 1104 10-11 0.5397034827452032 1104 12-13 0.5200632694946918 1104 14-15 0.5214437994898802 1104 16-17 0.5813361951951137 1104 18-19 0.5733950433701338 1104 20-21 0.488630748020185 1104 22-23 0.5153944529318295 1104 24-25 0.4552783204877642 1104 26-27 0.6027507219151289 1104 28-29 0.6664431623677203 1104 30-31 0.6710453372267651 1104 32-33 0.7217200480453059 1104 34-35 0.7643691350094386 1104 36-37 0.715674853296548 1104 38-39 0.6538188992209726 1104 40-41 0.6005446549291023 1104 42-43 0.55942827307004 1104 44-45 0.6861218906204982 1104 46-47 0.6863776562685544 1104 48-49 0.8213250159790775 1104 50-51 0.8135932818836906 1104 52-53 0.8465346260884772 1104 54-55 1.018184452034042 1104 56-57 1.0729379532370515 1104 58-59 0.9479987013652682 1104 60-61 1.106347392393971 1104 62-63 1.3300064348214242 1104 64-65 1.2842272721172705 1104 66-67 1.7587840530832999 1104 68-69 1.8198452902048743 1104 70-71 1.8300695923618377 1104 72-73 1.9364827168120655 1104 74-75 1.9977117421800017 1104 76-77 2.0543292939014215 1104 78-79 2.323575909974547 1104 80-81 2.5312000464106106 1104 82-83 2.7458588091522174 1104 84-85 2.830630140128161 1104 86-87 2.8502081258992114 1104 88-89 2.8616833159214927 1104 90-91 2.6650114874985427 1104 92-93 2.584180371347589 1104 94-95 2.6981412863036702 1104 96-97 2.5780648639216963 1104 98-99 2.4107208907388067 1104 100-101 1.7949494871032652 1105 1 0.47970065366539316 1105 2 0.30643356224537044 1105 3 0.25650846297201113 1105 4 0.27274153476344765 1105 5 0.24325199243287443 1105 6 0.2708854839426351 1105 7 0.3223092006452575 1105 8 0.3139740511850633 1105 9 0.3032502887438042 1105 10-11 0.5311902979846437 1105 12-13 0.5376821595063035 1105 14-15 0.5259318557193282 1105 16-17 0.5602403760709294 1105 18-19 0.5253572366531571 1105 20-21 0.52045917592649 1105 22-23 0.5597841130111121 1105 24-25 0.6663280187337293 1105 26-27 0.49218717358696296 1105 28-29 0.6798650322383608 1105 30-31 0.6462162682658672 1105 32-33 0.7410988048038476 1105 34-35 0.6096014684279254 1105 36-37 0.7409924651760775 1105 38-39 0.6791567336428486 1105 40-41 0.7998649415768995 1105 42-43 0.9401459441342013 1105 44-45 1.1691543349933369 1105 46-47 1.0976286699234876 1105 48-49 0.9539911289974796 1105 50-51 0.9375805538155433 1105 52-53 1.0954675499378013 1105 54-55 1.3044675689749283 1105 56-57 1.468049355852365 1105 58-59 1.4981671214387013 1105 60-61 1.7030441749200804 1105 62-63 1.6786541871195446 1105 64-65 1.7059640110166114 1105 66-67 1.8676580079991645 1105 68-69 2.1324028823202426 1105 70-71 2.0598841246852153 1105 72-73 1.9132861125813747 1105 74-75 1.9996160315834572 1105 76-77 2.2193447338124948 1105 78-79 2.2568882006291027 1105 80-81 2.3125271237881257 1105 82-83 2.6810689854051546 1105 84-85 2.7720858800608745 1105 86-87 2.787521278034074 1105 88-89 3.006617097206611 1105 90-91 3.111800080366862 1105 92-93 3.269675348405112 1105 94-95 3.210981589560916 1105 96-97 3.2014606264901744 1105 98-99 3.1975410846999495 1105 100-101 2.578352071409361 1106 1 0.15194908615523062 1106 2 0.08623804045680927 1106 3 -0.04614864545679964 1106 4 0.07985030397012594 1106 5 0.13393747602933104 1106 6 -0.1629087416290531 1106 7 0.18207133395680586 1106 8 -0.3019311561483633 1106 9 0.025027957595796124 1106 10-11 0.0858284941736045 1106 12-13 0.26061410131271145 1106 14-15 0.37729683199393094 1106 16-17 -0.18936715070099552 1106 18-19 0.3134540308310676 1106 20-21 0.36322990756737994 1106 22-23 0.11412378446371463 1106 24-25 0.2682359219803985 1106 26-27 0.16658314605596303 1106 28-29 0.19032224823138932 1106 30-31 -0.0042735573847920705 1106 32-33 0.26025000060474923 1106 34-35 0.352241489123827 1106 36-37 0.5258065084332344 1106 38-39 0.6244177811004548 1106 40-41 0.756902568628611 1106 42-43 0.21418722700767034 1106 44-45 0.6153906086421443 1106 46-47 0.8638303596347683 1106 48-49 0.8099960034264697 1106 50-51 0.5735784842255782 1106 52-53 0.753443981120526 1106 54-55 0.6450645503690424 1106 56-57 1.1067016715465243 1106 58-59 1.1658491806807127 1106 60-61 1.3690586484340912 1106 62-63 1.1729308177726274 1106 64-65 1.2641651016360562 1106 66-67 1.4280025538807486 1106 68-69 1.5633837590366753 1106 70-71 1.4282803286215184 1106 72-73 1.7577315782050782 1106 74-75 2.097361948568391 1106 76-77 2.311148068303165 1106 78-79 2.5152145559041763 1106 80-81 2.0997470653766683 1106 82-83 2.2018203691030536 1106 84-85 2.021283573557323 1106 86-87 1.7655536698401306 1106 88-89 1.594850048618902 1106 90-91 1.5616591576292862 1106 92-93 1.9109228209864533 1106 94-95 2.0233858540054968 1106 96-97 1.8307871928089625 1106 98-99 2.05145322243661 1106 100-101 1.400931025585642 1107 1 0.1557059530699938 1107 2 0.11522971155374506 1107 3 0.17926336942889876 1107 4 0.13343291369308474 1107 5 0.04708060320754015 1107 6 0.07633161515516207 1107 7 0.11100393481923021 1107 8 0.17793770793102937 1107 9 0.23202778038508853 1107 10-11 0.22818775933988888 1107 12-13 0.24884435708682417 1107 14-15 0.2887416899300135 1107 16-17 0.25812531784411163 1107 18-19 0.31538858101122713 1107 20-21 0.3506479476170057 1107 22-23 0.144033997707254 1107 24-25 0.35959394667175815 1107 26-27 0.24065131310658927 1107 28-29 0.39363904189905696 1107 30-31 0.38622794890618906 1107 32-33 0.5699493331111043 1107 34-35 0.4306926675750091 1107 36-37 0.5057196751885016 1107 38-39 0.7062773120827828 1107 40-41 0.7761417440081431 1107 42-43 0.7844867130966264 1107 44-45 0.7623012353773362 1107 46-47 0.7813328701197335 1107 48-49 0.6166473118321605 1107 50-51 0.6474842081313028 1107 52-53 0.6111142510714984 1107 54-55 0.6159901809437969 1107 56-57 0.7706511074257847 1107 58-59 0.7684305499287483 1107 60-61 1.078140696989827 1107 62-63 1.0550827464157813 1107 64-65 1.2013675353297195 1107 66-67 1.3370668813661304 1107 68-69 1.3564105178529076 1107 70-71 1.5861691584401747 1107 72-73 1.57741968736687 1107 74-75 1.9240469270077547 1107 76-77 2.032829797879632 1107 78-79 2.3739451365645827 1107 80-81 2.54223037803542 1107 82-83 2.529621774974629 1107 84-85 2.262532318314843 1107 86-87 2.29594825900665 1107 88-89 2.595827661000026 1107 90-91 2.2629941449291806 1107 92-93 2.5523546834709485 1107 94-95 2.5905044079661543 1107 96-97 2.7527022498117404 1107 98-99 2.521885616553213 1107 100-101 1.7178694153310499 1108 1 0.3052152302388471 1108 2 0.16871906571499196 1108 3 -0.009941105329886568 1108 4 -0.02419349346673272 1108 5 -0.03256743873986778 1108 6 0.09182561198547745 1108 7 0.11534245502188156 1108 8 0.10998871445011815 1108 9 0.24723773736436527 1108 10-11 0.21656757592052855 1108 12-13 0.14458822100911561 1108 14-15 0.1917549631373916 1108 16-17 0.24755583051090468 1108 18-19 0.2713052190853986 1108 20-21 0.20275336159251367 1108 22-23 0.23345313621187813 1108 24-25 0.2735612921433912 1108 26-27 0.3160418993221086 1108 28-29 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0.11228540238793627 2316 66-67 0.24799002255242897 2316 68-69 0.35626178744102077 2316 70-71 0.6016930270342193 2316 72-73 0.4414199658408329 2316 74-75 0.40999770462520146 2316 76-77 0.5124220866824452 2316 78-79 0.8792287580762732 2316 80-81 0.5613385440734113 2316 82-83 0.6698028252434014 2316 84-85 0.9506277673752024 2316 86-87 0.7481351234742171 2316 88-89 0.19679240450862423 2316 90-91 0.48496742830597483 2316 92-93 0.5965117934175304 2316 94-95 0.6646765808225368 2316 96-97 0.5627629655742084 2316 98-99 0.21830785902808714 2316 100-101 0.38587254605347887 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3721.0 3 1129.0 4 1113.0 5 1198.0 6 1455.0 7 1896.0 8 2573.0 9 3346.0 10 4622.0 11 6421.0 12 8725.0 13 11796.0 14 13925.0 15 16694.0 16 20680.0 17 25405.0 18 23796.0 19 21054.0 20 22571.0 21 25663.0 22 28748.0 23 32874.0 24 37031.0 25 42228.0 26 48303.0 27 54933.0 28 62365.0 29 71206.0 30 80940.0 31 92881.0 32 106809.0 33 124147.0 34 143768.0 35 158158.0 36 151979.0 37 108645.0 38 28294.0 39 441.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.13373678461605 18.311521065151748 13.507881931218199 23.046860219014007 2 15.589782331198734 20.21435660023619 39.94885257688612 24.247008491678958 3 19.294036602390086 25.28785554142738 29.22672626900626 26.191381587176277 4 12.471768645265838 15.895737749022079 36.42465489074238 35.20783871496971 5 13.328844385010935 38.18894197248413 33.45549056849324 15.026723074011691 6 32.303277194173596 35.67131244931445 17.217685406961756 14.807724949550193 7 28.7762277760807 33.474128296896346 20.149582077806816 17.600061849216136 8 27.455745231321604 33.88706532520545 19.637853243003025 19.019336200469922 9 27.717259104357154 15.983655244068236 19.8396700375683 36.45941561400631 10-11 25.200345582183093 26.708606272335476 26.796573217632236 21.29447492784919 12-13 26.38292202427473 24.174828583253436 27.57752053088518 21.864728861586652 14-15 22.79165035205005 25.214771583844982 26.486871735374507 25.506706328730466 16-17 21.72824710145178 28.6111070086467 27.26556851201102 22.395077377890495 18-19 21.830535126925664 26.350999947520865 30.494746272802892 21.323718652750582 20-21 22.26397127570141 25.67374036565341 30.72890264694097 21.33338571170421 22-23 21.826387012023808 25.855180042865854 30.705683810912564 21.612749134197774 24-25 21.542742686490985 26.13763687833327 30.829741755242164 21.489878679933582 26-27 21.111804964758555 26.497034639918766 30.58816615319052 21.802994242132158 28-29 21.16971212090262 26.95714889544997 30.52040106031775 21.352737923329663 30-31 21.23466676052868 26.481657938337687 30.40384339793889 21.879831903194745 32-33 20.830315187164867 26.66591455249783 30.361319627257245 22.142450633080056 34-35 21.72319624400162 26.78420921966871 30.151063288195566 21.3415312481341 36-37 21.866035624945006 26.337066787343964 30.01049641110734 21.786401176603686 38-39 21.320179590877427 26.643429659139933 30.148524760044324 21.88786598993831 40-41 21.670027593323056 26.545424039182393 30.22144411550863 21.56310425198592 42-43 21.465806878119853 26.626014919046842 29.84151851150129 22.066659691332017 44-45 21.2884422697894 27.00498660425478 30.054572698192462 21.65199842776336 46-47 21.89540399096918 27.26786250151221 29.17256754654848 21.664165960970138 48-49 21.60859268485581 27.393063549305722 28.912707881581927 22.08563588425654 50-51 21.842252770593127 27.66932729068794 28.823343111158028 21.6650768275609 52-53 22.136919383004408 27.30699407875761 28.82770962976369 21.728376908474296 54-55 21.316577091843882 27.5871917114855 29.216567477709976 21.879663718960643 56-57 21.57194308007416 27.862561962204822 28.702140608215377 21.863354349505638 58-59 21.471498879182644 27.262896468522076 28.964151124194732 22.30145352810055 60-61 21.15271750673297 27.58171622611727 29.239321814075716 22.026244453074042 62-63 21.37779027588168 27.46069773981566 29.374824245413883 21.786687738888773 64-65 21.04925822512543 27.579214657103297 29.182625951234105 22.18890116653717 66-67 21.04647049324435 27.65656309241129 29.077156337830818 22.21981007651354 68-69 21.642727723147114 27.183071731373605 29.171014132129645 22.00318641334963 70-71 21.369934032649045 27.392773597977776 28.804779687271708 22.432512682101464 72-73 21.310612653996124 27.539518209324758 28.756468544102354 22.393400592576768 74-75 21.28679787010426 27.895544996356858 28.59473319904446 22.222923934494425 76-77 21.417321548240263 27.442410060539302 28.75916894420827 22.381099447012165 78-79 21.560393142811183 27.623963086149704 28.530062882490114 22.285580888549003 80-81 21.638682591495343 27.777175564292367 28.23603082034359 22.348111023868704 82-83 21.47854144207467 27.836724613628444 28.168243615407796 22.5164903288891 84-85 21.496395635552005 27.682159904084696 28.20572274544993 22.615721714913374 86-87 21.662414384933445 27.741967711562403 27.928584287072844 22.667033616431308 88-89 21.55121381035274 27.64565410018413 27.810993734564214 22.992138354898916 90-91 21.553557315668655 27.654218955485828 27.856246140696268 22.93597758814925 92-93 21.847842582296824 27.196480239996205 28.020210405134982 22.93546677257199 94-95 21.535258822364916 26.99470053214639 28.191503568853015 23.27853707663568 96-97 21.884272507516986 26.552643726124987 27.966958883052385 23.596124883305645 98-99 21.686353293522295 26.76537105893043 27.54360917233219 24.004666475215082 100-101 22.463567993306512 25.864412857282154 27.837041277550085 23.83497787186125 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2540.0 1 1884.0 2 2101.5 3 2659.5 4 1833.0 5 1539.0 6 2468.5 7 4588.0 8 6686.0 9 6736.0 10 4995.5 11 3551.5 12 3117.5 13 3066.5 14 3106.5 15 3123.5 16 3102.0 17 3154.5 18 3318.5 19 3528.5 20 3862.0 21 4381.5 22 5145.0 23 6152.5 24 7282.0 25 8605.5 26 10343.0 27 12647.5 28 14745.0 29 16737.0 30 19165.0 31 22640.5 32 27592.5 33 29348.5 34 28903.5 35 30451.5 36 33362.5 37 36432.5 38 38885.0 39 40922.5 40 42501.0 41 44763.0 42 47091.0 43 49142.5 44 50740.0 45 53740.5 46 56094.5 47 56789.5 48 57600.5 49 56358.0 50 54861.0 51 53339.5 52 51586.0 53 51702.0 54 51752.5 55 48964.5 56 45318.5 57 41646.5 58 38335.0 59 34289.5 60 28479.5 61 23821.0 62 20586.5 63 16228.5 64 11782.5 65 9090.0 66 7136.5 67 5687.5 68 4539.5 69 3400.5 70 2553.5 71 1931.0 72 1487.0 73 1234.0 74 968.0 75 771.0 76 589.0 77 389.5 78 267.5 79 181.0 80 106.0 81 78.0 82 60.0 83 40.0 84 26.0 85 17.0 86 12.0 87 6.0 88 5.5 89 3.0 90 1.5 91 1.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36951794276336086 2 0.028023295778347043 3 0.03418088094937397 4 0.041469451151814 5 0.06031920167536583 6 0.05296779897118062 7 0.03562602848951294 8 0.039647308601203995 9 0.035437530984277425 10-11 0.03735392228750519 12-13 0.02855737204318101 14-15 0.02664098073995324 16-17 0.021897126858192697 18-19 0.026672396990825825 20-21 0.03427512970199172 22-23 0.03286139841272534 24-25 0.041375202399196245 26-27 0.036411434761327605 28-29 0.03144766712345896 30-31 0.034243713451119144 32-33 0.02906003205714239 34-35 0.030033935834192567 36-37 0.03223307339527361 38-39 0.015676709185420596 40-41 0.012943495359505583 42-43 0.013289074119104034 44-45 0.010650109045806778 46-47 0.019823654300601998 48-49 0.017121856725559572 50-51 0.011121352808895573 52-53 0.009047880251304874 54-55 0.007916895219891765 56-57 0.008765133993451597 58-59 0.0038013663555829503 60-61 0.006974407693714173 62-63 0.011341266565003678 64-65 0.007131488948077105 66-67 0.003141625087258637 68-69 0.0055606764044477865 70-71 0.013540404126084725 72-73 0.011184185310640747 74-75 0.011781094077219889 76-77 0.005497843902702615 78-79 0.009550540265266255 80-81 0.01140409906674885 82-83 0.01391739913655576 84-85 0.008105392725127284 86-87 0.0021677213102084592 88-89 0.01652494795898043 90-91 0.025792741966393404 92-93 0.010964271554532641 94-95 0.021080304335505454 96-97 0.019069664279659924 98-99 0.011435515317621438 100-101 0.12114106336469303 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1591533.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.404387517859384 #Duplication Level Percentage of deduplicated Percentage of total 1 76.72456127490952 40.974282024630156 2 12.918639227020309 13.798240309664243 3 4.330065461704239 6.937334816836556 4 2.021144922046525 4.317520265869053 5 1.0514026880824938 2.8074758295838267 6 0.6486532443891514 2.0784557536844996 7 0.4332612722885489 1.6196637017244917 8 0.30257125018871983 1.2926905837473264 9 0.22607251058633332 1.0865937566239123 >10 1.2111447728714693 11.793185329439318 >50 0.08458294646437893 3.0589022251159506 >100 0.04058576587916733 4.110874632962889 >500 0.0037753102292738404 1.4454129785749492 >1k 0.0031854180059498025 2.9685749487178987 >5k 2.3595688932961503E-4 0.947227179281452 >10k+ 1.1797844466480751E-4 0.7635656635434294 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12119 0.7614670886497483 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8222 0.5166088293488103 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6812 0.42801500188811664 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3773 0.23706702908453675 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3072 0.19302144536117066 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2893 0.18177442754878473 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2564 0.16110253447462292 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2465 0.1548821168018508 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2426 0.15243164923378907 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2248 0.1412474639231483 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2129 0.13377039621547276 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2023 0.12711015103048443 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1836 0.11536047320413714 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1741 0.10939138553834574 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1721 0.10813473550344227 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 1608 0.10103466280623775 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.283250174517274E-5 7 0.0 0.0 0.0 0.0 6.283250174517274E-5 8 0.0 0.0 0.0 0.0 6.283250174517274E-5 9 0.0 0.0 0.0 0.0 1.2566500349034547E-4 10-11 0.0 0.0 0.0 0.0 1.2566500349034547E-4 12-13 0.0 0.0 0.0 0.0 1.2566500349034547E-4 14-15 0.0 0.0 0.0 0.0 1.2566500349034547E-4 16-17 0.0 0.0 0.0 3.141625087258637E-5 1.2566500349034547E-4 18-19 0.0 0.0 0.0 6.283250174517274E-5 1.2566500349034547E-4 20-21 0.0 0.0 0.0 1.8849750523551823E-4 1.2566500349034547E-4 22-23 0.0 0.0 0.0 5.340762648339682E-4 1.2566500349034547E-4 24-25 3.141625087258637E-5 0.0 0.0 0.0011624012822856957 1.2566500349034547E-4 26-27 6.283250174517274E-5 0.0 0.0 0.0021363050593358733 1.2566500349034547E-4 28-29 6.283250174517274E-5 0.0 0.0 0.004492523874779851 1.2566500349034547E-4 30-31 6.283250174517274E-5 0.0 0.0 0.01460855665575266 1.2566500349034547E-4 32-33 6.283250174517274E-5 0.0 0.0 0.0322644896461462 1.2566500349034547E-4 34-35 6.283250174517274E-5 0.0 0.0 0.06028778542449324 1.2566500349034547E-4 36-37 6.283250174517274E-5 0.0 0.0 0.10675242046504849 1.2566500349034547E-4 38-39 6.283250174517274E-5 0.0 0.0 0.18120893503307817 1.2566500349034547E-4 40-41 6.283250174517274E-5 0.0 0.0 0.2668810511626212 1.2566500349034547E-4 42-43 6.283250174517274E-5 0.0 0.0 0.35393548233055805 1.2566500349034547E-4 44-45 6.283250174517274E-5 0.0 0.0 0.4605308215412436 1.2566500349034547E-4 46-47 6.283250174517274E-5 0.0 0.0 0.5851905050036663 1.2566500349034547E-4 48-49 6.283250174517274E-5 0.0 0.0 0.7232335113378108 1.2566500349034547E-4 50-51 6.283250174517274E-5 0.0 0.0 0.8839904670528352 1.2566500349034547E-4 52-53 6.283250174517274E-5 0.0 0.0 1.033626069958964 1.5708125436293184E-4 54-55 6.283250174517274E-5 0.0 0.0 1.193817533158282 1.884975052355182E-4 56-57 6.283250174517274E-5 0.0 0.0 1.384074348442665 1.884975052355182E-4 58-59 6.283250174517274E-5 0.0 0.0 1.594468980536376 1.884975052355182E-4 60-61 6.283250174517274E-5 0.0 0.0 1.8345519697046808 2.199137561081046E-4 62-63 6.283250174517274E-5 0.0 0.0 2.0652414998620827 2.5133000698069095E-4 64-65 6.283250174517274E-5 0.0 0.0 2.2995753151207046 2.5133000698069095E-4 66-67 6.283250174517274E-5 0.0 0.0 2.568341341335681 2.5133000698069095E-4 68-69 6.283250174517274E-5 0.0 0.0 2.8599783981859 2.5133000698069095E-4 70-71 6.283250174517274E-5 0.0 0.0 3.1819007208772927 3.1416250872586367E-4 72-73 6.283250174517274E-5 0.0 0.0 3.509415136224005 3.1416250872586367E-4 74-75 6.283250174517274E-5 0.0 0.0 3.832845438957282 3.1416250872586367E-4 76-77 6.283250174517274E-5 0.0 0.0 4.177041883517338 3.1416250872586367E-4 78-79 6.283250174517274E-5 0.0 0.0 4.568708283145872 3.1416250872586367E-4 80-81 6.283250174517274E-5 0.0 0.0 4.988932054817587 3.1416250872586367E-4 82-83 6.283250174517274E-5 0.0 0.0 5.420779839312161 3.1416250872586367E-4 84-85 6.283250174517274E-5 0.0 0.0 5.8445850635833505 3.1416250872586367E-4 86-87 6.283250174517274E-5 0.0 0.0 6.28636038335366 3.1416250872586367E-4 88-89 6.283250174517274E-5 0.0 0.0 6.737183583375274 3.1416250872586367E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4690 0.0 47.888855 1 GTATCAA 8115 0.0 40.722187 1 ACCGTAT 75 3.0762708E-4 31.671415 8 TATCAAC 10530 0.0 31.231054 2 ATCAACG 10620 0.0 30.876886 3 TCAACGC 10850 0.0 30.222353 4 CAACGCA 10970 0.0 29.935074 5 AACGCAG 11260 0.0 29.20355 6 GTACATG 9435 0.0 28.858953 1 TACATGG 9345 0.0 28.732807 2 TACCGTA 85 6.4026547E-4 27.945364 7 ACATGGG 9355 0.0 27.58449 3 ACGCAGA 12770 0.0 25.557865 7 CGCAGAG 12990 0.0 25.088442 8 CATGGGG 3695 0.0 24.822859 4 GTAAGGT 645 0.0 23.577576 4 GGACCGA 345 0.0 23.415195 6 CATGGGA 5660 0.0 23.090067 4 GCAGAGT 13950 0.0 23.020647 9 TAAGGTG 765 0.0 22.985249 5 >>END_MODULE