FastQCFastQC Report
Thu 26 May 2016
SRR938312_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938312_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591533
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120210.7553095034787215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89010.5592720980337825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74760.4697357830469114No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43110.2708709150234397No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27740.17429735984110917No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA27070.1700875822241826No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25540.16047420945717117No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24300.15268297924076976No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21860.13735184881494758No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG21380.1343358887311793No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19480.1223977133995965No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA19010.11944458581757338No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG18540.11649145823555025No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17740.11146485809593644No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC16700.10493027791443846No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47950.048.0568431
GTATCAA89100.043.0326271
TATCAAC114450.033.286272
ATCAACG116800.032.5331733
TCAACGC120200.031.6514514
CAACGCA122300.031.0691325
TGTACCG1252.1560118E-730.398045
AACGCAG126450.030.1245886
GTACCGT1303.0398405E-729.2288826
GTACATG90050.027.225011
TACATGG90100.026.8348732
ACGCAGA141600.026.767347
CGCAGAG141800.026.6960938
TATAACG1109.4769544E-525.9098632
TACCGTA1109.483029E-525.9074217
ACATGGG90350.025.3395063
GCAGAGT149200.024.8945069
GAGTACT94250.022.95475412-13
CATGGGA57200.022.6689954
AGTACTT96300.022.58940512-13
Produced by FastQC (version 0.11.5)