##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938312_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1591533 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.564953412841582 31.0 31.0 34.0 30.0 34.0 2 31.822599971222715 31.0 31.0 34.0 30.0 34.0 3 31.866969142330067 31.0 31.0 34.0 30.0 34.0 4 35.585422985260124 37.0 35.0 37.0 33.0 37.0 5 35.48295008648894 37.0 35.0 37.0 33.0 37.0 6 35.53016996820047 37.0 35.0 37.0 33.0 37.0 7 35.43855515405587 37.0 35.0 37.0 33.0 37.0 8 35.41420378967951 37.0 35.0 37.0 33.0 37.0 9 36.890301991853136 39.0 37.0 39.0 33.0 39.0 10-11 36.81249242082947 39.0 37.0 39.0 32.0 39.0 12-13 36.67373469478798 39.0 35.0 39.0 32.0 39.0 14-15 37.540038126762056 39.0 36.0 41.0 32.0 41.0 16-17 37.639371913746054 39.0 36.5 41.0 32.0 41.0 18-19 37.814601079588044 39.5 37.0 41.0 32.0 41.0 20-21 37.771733291109896 40.0 37.0 41.0 32.0 41.0 22-23 37.60797608343654 39.0 36.5 41.0 32.0 41.0 24-25 37.43880020081268 39.0 36.0 41.0 32.0 41.0 26-27 37.156638285225625 39.0 36.0 41.0 31.0 41.0 28-29 36.97425312575988 39.0 36.0 40.0 31.0 41.0 30-31 36.61522852495047 38.5 35.0 40.0 30.0 41.0 32-33 36.37700380702128 38.0 35.0 40.0 30.0 41.0 34-35 36.16475404531354 38.0 35.0 40.0 30.0 41.0 36-37 35.964774528709114 38.0 35.0 40.0 29.5 41.0 38-39 35.67190249903709 38.0 34.5 40.0 28.5 41.0 40-41 35.363737352602804 38.0 34.0 40.0 27.0 41.0 42-43 35.000841955523384 38.0 34.0 40.0 26.5 41.0 44-45 34.58745938664168 38.0 33.0 40.0 24.5 41.0 46-47 34.00543626805099 37.5 33.0 40.0 23.5 41.0 48-49 33.44267351038276 37.0 32.5 40.0 21.5 41.0 50-51 32.94806893730761 37.0 31.5 39.5 19.5 41.0 52-53 32.239549541228484 36.0 31.0 39.0 16.0 40.0 54-55 31.706605832238477 35.0 30.0 39.0 12.5 40.0 56-57 31.23324681297843 35.0 29.5 38.5 8.5 40.0 58-59 30.600308004923555 35.0 28.5 38.0 7.5 40.0 60-61 29.99843452821902 34.0 28.0 38.0 4.0 40.0 62-63 29.3929937362279 34.0 26.5 37.5 2.0 40.0 64-65 28.627703918171978 33.5 26.0 37.0 2.0 39.0 66-67 27.863745520828033 33.0 25.0 36.0 2.0 39.0 68-69 27.031611345790505 32.0 22.5 36.0 2.0 39.0 70-71 26.15230378509274 31.0 21.0 35.0 2.0 38.0 72-73 25.48789877432639 31.0 19.5 35.0 2.0 37.5 74-75 24.704677502759917 30.0 17.5 34.0 2.0 36.5 76-77 23.700782201814224 29.5 16.0 33.5 2.0 35.5 78-79 24.11665984934023 30.0 13.5 34.0 2.0 36.0 80-81 23.657292057406288 30.0 7.5 34.0 2.0 35.5 82-83 22.863967947884213 29.5 3.5 34.0 2.0 35.0 84-85 21.96634345627769 29.0 2.0 34.0 2.0 35.0 86-87 20.932399453859894 28.0 2.0 33.0 2.0 35.0 88-89 19.805448269058825 26.0 2.0 33.0 2.0 34.5 90-91 18.809525784259577 25.0 2.0 32.0 2.0 34.0 92-93 17.618877522489324 21.5 2.0 32.0 2.0 34.0 94-95 16.348602887907447 14.5 2.0 31.0 2.0 34.0 96-97 14.909092051500032 2.0 2.0 31.0 2.0 34.0 98-99 13.2080252184529 2.0 2.0 30.5 2.0 34.0 100-101 9.849408400579819 2.0 2.0 23.0 2.0 31.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30376692006816697 1101 2 0.31045389352193453 1101 3 0.33920741022032175 1101 4 0.1855930814621729 1101 5 0.09524603804197085 1101 6 0.2531927977548918 1101 7 0.2706004881818629 1101 8 0.28562063195095533 1101 9 0.3754391641970187 1101 10-11 0.42722474198521354 1101 12-13 0.4238767466391451 1101 14-15 0.529705016488542 1101 16-17 0.4862718371679833 1101 18-19 0.4755271658070228 1101 20-21 0.5569216156813823 1101 22-23 0.5247399789430389 1101 24-25 0.49192523593336546 1101 26-27 0.6047588252766687 1101 28-29 0.6567916850051603 1101 30-31 0.6928902038257831 1101 32-33 0.36228719466122783 1101 34-35 0.3290434467449188 1101 36-37 0.520651026002561 1101 38-39 0.5607266526862986 1101 40-41 0.6739271939652269 1101 42-43 0.8655566656909457 1101 44-45 0.782199654381337 1101 46-47 0.7550218080856013 1101 48-49 0.8094254290244081 1101 50-51 1.0111699018208142 1101 52-53 1.1696223929551692 1101 54-55 1.1591300832474687 1101 56-57 1.4846702183902103 1101 58-59 1.6471720487647907 1101 60-61 1.5121782062729032 1101 62-63 1.6022664676136493 1101 64-65 1.5936156060676048 1101 66-67 1.8661240408108135 1101 68-69 2.235750093481826 1101 70-71 2.2629466562968865 1101 72-73 2.43765852601069 1101 74-75 2.6706537930241474 1101 76-77 2.6805742552817193 1101 78-79 2.7088297467433193 1101 80-81 3.0177321885410286 1101 82-83 3.1720040518132357 1101 84-85 3.273139782504593 1101 86-87 3.2691214811477707 1101 88-89 3.1574084690145803 1101 90-91 3.1136856877477967 1101 92-93 3.50172887090849 1101 94-95 3.0577411406952884 1101 96-97 2.9969819869944985 1101 98-99 2.837970381849324 1101 100-101 2.1846869095116315 1102 1 0.2968801890540611 1102 2 0.24476769158343714 1102 3 0.29587981177943945 1102 4 0.20832254632268388 1102 5 0.2028077965529036 1102 6 0.3363244378369288 1102 7 0.2672578758374158 1102 8 0.3110126720398725 1102 9 0.299584930657538 1102 10-11 0.37347377090417666 1102 12-13 0.4908121756382613 1102 14-15 0.6156369963274457 1102 16-17 0.4456825190220215 1102 18-19 0.3810162130596879 1102 20-21 0.3346917294894567 1102 22-23 0.5152815187803554 1102 24-25 0.3924065332303357 1102 26-27 0.5558301734698716 1102 28-29 0.5099892800888526 1102 30-31 0.5977584695329909 1102 32-33 0.6346426204153062 1102 34-35 0.6803289748522587 1102 36-37 0.8120813246282026 1102 38-39 0.6627617544152145 1102 40-41 0.7525092399385755 1102 42-43 0.7206467005115371 1102 44-45 0.9525861502424178 1102 46-47 0.9691632154297025 1102 48-49 0.979645373739146 1102 50-51 1.046812968694745 1102 52-53 0.9434952847092575 1102 54-55 1.1361159299516217 1102 56-57 1.321151013229855 1102 58-59 1.3391498165233315 1102 60-61 1.2894402549679 1102 62-63 1.369453386974481 1102 64-65 1.4151456783631353 1102 66-67 1.7279574343343462 1102 68-69 1.7027845559235146 1102 70-71 2.0193047603279872 1102 72-73 2.0078588061007387 1102 74-75 2.054508762304714 1102 76-77 2.0772319794042318 1102 78-79 2.127693542673864 1102 80-81 2.476492928379539 1102 82-83 2.473639767257893 1102 84-85 2.5765735400106102 1102 86-87 2.6596534503692695 1102 88-89 2.4534864979462547 1102 90-91 2.558207893445754 1102 92-93 2.6876276152885588 1102 94-95 2.531149969737921 1102 96-97 2.5380732016925727 1102 98-99 2.540703689910252 1102 100-101 2.247779698875087 1103 1 0.18843665272018129 1103 2 0.27747058662244584 1103 3 0.2618918820823275 1103 4 0.15762989372300495 1103 5 0.15345548021608124 1103 6 0.2129822125876757 1103 7 0.21024483193521348 1103 8 0.29296834052712484 1103 9 0.4152227691797634 1103 10-11 0.4240085228691939 1103 12-13 0.3972153010521211 1103 14-15 0.4652290014617577 1103 16-17 0.44672015176406177 1103 18-19 0.4664208097872873 1103 20-21 0.4659722818069625 1103 22-23 0.45624378125632603 1103 24-25 0.5186804711848438 1103 26-27 0.5393200976630226 1103 28-29 0.5157541085592783 1103 30-31 0.5588425119638742 1103 32-33 0.6489759231872299 1103 34-35 0.8097652914160989 1103 36-37 0.7508449844884098 1103 38-39 0.7259241912367713 1103 40-41 0.945951342796647 1103 42-43 0.8916217987810384 1103 44-45 1.0067751740381183 1103 46-47 1.2388553164686442 1103 48-49 1.4967808670367404 1103 50-51 1.4193642354653235 1103 52-53 1.5763173692595913 1103 54-55 1.6005635469618156 1103 56-57 1.6765056638765152 1103 58-59 1.5432603993585836 1103 60-61 1.6238570631630154 1103 62-63 2.106062394441654 1103 64-65 2.1719805201205027 1103 66-67 1.9389177758072016 1103 68-69 1.956859774837831 1103 70-71 2.238650571449721 1103 72-73 2.227820939054528 1103 74-75 2.1285143157500777 1103 76-77 2.2008532594617485 1103 78-79 2.586892964626564 1103 80-81 2.776105744380917 1103 82-83 3.0684365411369043 1103 84-85 3.125774140760676 1103 86-87 2.892615098706365 1103 88-89 2.810139280999511 1103 90-91 3.2365381051490303 1103 92-93 3.0601432270771163 1103 94-95 2.586342426599032 1103 96-97 2.578189786207254 1103 98-99 2.4170493016551475 1103 100-101 2.020869210343621 1104 1 0.2600652700735395 1104 2 0.21655280668064947 1104 3 0.19893676897651957 1104 4 0.20420352309538004 1104 5 0.12425577532572163 1104 6 0.09105555244065755 1104 7 0.19802738940947506 1104 8 0.24157467903499708 1104 9 0.20839480443556369 1104 10-11 0.2305664305446058 1104 12-13 0.1990570447111608 1104 14-15 0.31367939978385806 1104 16-17 0.438429510176654 1104 18-19 0.4546191847518344 1104 20-21 0.40554002486005203 1104 22-23 0.3859052333531139 1104 24-25 0.46018305960559047 1104 26-27 0.5271511384214165 1104 28-29 0.39842921948419985 1104 30-31 0.4152847154031889 1104 32-33 0.45633578924223883 1104 34-35 0.3910741744509565 1104 36-37 0.43687638095192227 1104 38-39 0.511340281337695 1104 40-41 0.574961911117363 1104 42-43 0.6903305905775952 1104 44-45 0.4275672908286552 1104 46-47 0.8176484174180914 1104 48-49 0.6931666366234666 1104 50-51 0.7361883491529255 1104 52-53 0.7739949336802425 1104 54-55 0.9940999487636688 1104 56-57 1.0660391753154705 1104 58-59 1.101914475784806 1104 60-61 1.0647656019493006 1104 62-63 1.1800087198228049 1104 64-65 1.2964098494362268 1104 66-67 1.3565943671114056 1104 68-69 1.5635706205312374 1104 70-71 1.6842518090329968 1104 72-73 1.801859745006137 1104 74-75 1.7861624589265652 1104 76-77 2.1527436934509794 1104 78-79 2.3063742038386046 1104 80-81 2.3358918451032977 1104 82-83 2.488906701314633 1104 84-85 2.924535556762436 1104 86-87 2.516138083923966 1104 88-89 2.4160913029077484 1104 90-91 2.804823273819096 1104 92-93 3.000246701792996 1104 94-95 3.023659406514877 1104 96-97 2.8603747501087717 1104 98-99 2.879285136771953 1104 100-101 2.403855753040313 1105 1 0.09959467803164657 1105 2 0.16487881833018392 1105 3 0.21435275004283127 1105 4 0.21872735300222956 1105 5 0.13899733460507235 1105 6 0.15068037035351267 1105 7 0.20419863524615778 1105 8 0.16400639930601102 1105 9 0.35725698309094156 1105 10-11 0.38633443888707575 1105 12-13 0.19301758244203882 1105 14-15 0.47578296211951 1105 16-17 0.4453811776263805 1105 18-19 0.39301727628311056 1105 20-21 0.40545862912713915 1105 22-23 0.3234126904156014 1105 24-25 0.25170533170259546 1105 26-27 0.259609094070683 1105 28-29 0.33877086585530236 1105 30-31 0.3509005221511785 1105 32-33 0.6469258358417065 1105 34-35 0.6884129552878022 1105 36-37 0.69391534237608 1105 38-39 0.7253188777988839 1105 40-41 0.8425509728790246 1105 42-43 0.8130753558039885 1105 44-45 1.109372159473132 1105 46-47 1.1461636176920393 1105 48-49 1.135062553894393 1105 50-51 1.129922428113872 1105 52-53 1.0909055965549541 1105 54-55 1.2500194239482454 1105 56-57 1.4172337882998285 1105 58-59 1.494851281014089 1105 60-61 1.6350763482757067 1105 62-63 1.4262402490581998 1105 64-65 1.7463085007948784 1105 66-67 1.686329323730682 1105 68-69 2.044563621509333 1105 70-71 1.8913213739045496 1105 72-73 1.9695543347345712 1105 74-75 2.025325198487856 1105 76-77 1.9892651667575585 1105 78-79 2.252881703368427 1105 80-81 2.163047416447416 1105 82-83 2.5546281101787116 1105 84-85 2.629901372442049 1105 86-87 2.653436507307159 1105 88-89 2.584701131198141 1105 90-91 2.9243174338859106 1105 92-93 2.9417966450687665 1105 94-95 2.795542219578369 1105 96-97 2.8818632235966817 1105 98-99 2.72610291750971 1105 100-101 2.1884015850732244 1106 1 0.03831711470151333 1106 2 0.08463659461023454 1106 3 0.0626300206349093 1106 4 0.0317657697659115 1106 5 0.06171789230510427 1106 6 -0.01054273008286799 1106 7 0.0059700408653640125 1106 8 0.03036875455384802 1106 9 -0.0831796731596981 1106 10-11 0.0806873175914049 1106 12-13 0.08437942710025226 1106 14-15 0.16143096843720883 1106 16-17 0.041550393837262334 1106 18-19 2.887533848650037E-4 1106 20-21 -0.05699260956330221 1106 22-23 0.15889696765895422 1106 24-25 0.12556314270273816 1106 26-27 0.016668165886812858 1106 28-29 0.33479167068071547 1106 30-31 0.2175001313799143 1106 32-33 0.20376092900730214 1106 34-35 0.20433450148935606 1106 36-37 0.17099463354151823 1106 38-39 0.26111018960575194 1106 40-41 0.3486920045423858 1106 42-43 0.523169031981233 1106 44-45 0.4519852891152425 1106 46-47 0.09400416395486388 1106 48-49 0.34102317722221187 1106 50-51 0.434329837790564 1106 52-53 0.19008933393488547 1106 54-55 -0.019195425359733065 1106 56-57 0.42133416341300745 1106 58-59 0.6329484155850871 1106 60-61 0.9128160020278564 1106 62-63 0.8078474490527547 1106 64-65 1.2485047169853338 1106 66-67 1.1866618877756387 1106 68-69 0.8818540244666693 1106 70-71 0.7022942854227701 1106 72-73 1.0001825642139721 1106 74-75 1.23060605682306 1106 76-77 1.2329546193374021 1106 78-79 1.5341648491451707 1106 80-81 1.5931306765962354 1106 82-83 1.8217825601375246 1106 84-85 2.0195592093120673 1106 86-87 2.1430671127303 1106 88-89 2.1087110979076975 1106 90-91 2.145008227614511 1106 92-93 1.7729177637366043 1106 94-95 1.5295144707340036 1106 96-97 2.102031173545285 1106 98-99 2.413051225415682 1106 100-101 2.2079782722315553 1107 1 -0.030440638266764353 1107 2 0.07885952567526999 1107 3 0.02611870868500077 1107 4 0.05353905820586391 1107 5 0.011153771565545867 1107 6 -0.010076075230252002 1107 7 0.02168863026816581 1107 8 0.12762198864918872 1107 9 0.15926229033490102 1107 10-11 0.20269393345766673 1107 12-13 0.08705235521177457 1107 14-15 0.08792396861441176 1107 16-17 0.10498001165284876 1107 18-19 0.10767843972191571 1107 20-21 0.09083065229504683 1107 22-23 0.15229882246432425 1107 24-25 0.25907073322786545 1107 26-27 0.1821297997694984 1107 28-29 0.27055279065235993 1107 30-31 0.330090951865472 1107 32-33 0.2832871857265431 1107 34-35 0.37819592271920044 1107 36-37 0.5763818389287181 1107 38-39 0.6334978084845986 1107 40-41 0.6885053426013457 1107 42-43 0.603994833859673 1107 44-45 0.6307259117155155 1107 46-47 0.668072331005483 1107 48-49 0.89008986751697 1107 50-51 0.81269831791343 1107 52-53 0.836790261337562 1107 54-55 0.9168876273899897 1107 56-57 0.903152345231188 1107 58-59 0.9941953882355712 1107 60-61 1.249285964813609 1107 62-63 1.553688904543332 1107 64-65 1.6797853596694843 1107 66-67 1.882429505473084 1107 68-69 2.0677775875832154 1107 70-71 1.9689816266715177 1107 72-73 2.074215289123888 1107 74-75 1.9996562074521584 1107 76-77 2.2675077530133407 1107 78-79 2.4150851633988175 1107 80-81 2.5827786179985637 1107 82-83 2.650771277722736 1107 84-85 2.7620454754825445 1107 86-87 3.1990893231384767 1107 88-89 3.013737974864398 1107 90-91 3.057005392243255 1107 92-93 2.942505453499731 1107 94-95 2.6357552410098606 1107 96-97 2.4818084787611845 1107 98-99 2.2022797681529944 1107 100-101 1.9968701146321681 1108 1 0.060215833128786755 1108 2 0.09031035347548055 1108 3 0.09133476120975104 1108 4 0.07398301500666804 1108 5 -0.013994438166463397 1108 6 0.11253802144133118 1108 7 -0.03082229458318153 1108 8 -0.10927290278097956 1108 9 -0.04406059887824654 1108 10-11 0.04455443218640198 1108 12-13 0.06608902023202035 1108 14-15 0.24893874497073654 1108 16-17 0.1515292416660543 1108 18-19 0.021689773666061285 1108 20-21 0.05788234377750001 1108 22-23 0.15571169952408326 1108 24-25 0.2424537735351393 1108 26-27 0.27046933547745766 1108 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0.7874704964039374 2316 90-91 0.9213031315409879 2316 92-93 1.1384983819488 2316 94-95 1.0632655150113628 2316 96-97 0.932649385742442 2316 98-99 0.9367980925836008 2316 100-101 0.5646616473359831 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 18.0 4 48.0 5 130.0 6 299.0 7 647.0 8 1338.0 9 2311.0 10 3692.0 11 5594.0 12 8166.0 13 11284.0 14 14638.0 15 19568.0 16 24297.0 17 30092.0 18 29265.0 19 26543.0 20 27491.0 21 29757.0 22 32441.0 23 35815.0 24 40090.0 25 44375.0 26 50462.0 27 58032.0 28 66443.0 29 75590.0 30 86209.0 31 97218.0 32 109685.0 33 124176.0 34 141024.0 35 151910.0 36 137580.0 37 87740.0 38 17383.0 39 175.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.328466988142914 19.896794936654764 14.63240659099636 22.142331484205954 2 16.457130361179136 21.751873070453087 38.43629172581378 23.354704842554 3 19.585676495906405 25.873290145836343 30.074834591048756 24.4661987672085 4 13.345560538172943 17.218493113243646 35.914366840021536 33.52157950856187 5 13.064958794419464 38.57459353629082 33.62879288635101 14.731654782938703 6 31.97765927567949 36.686703951473206 16.69352756116273 14.642109211684584 7 28.33437949448739 33.864770633094004 20.146676192073933 17.654173680344673 8 27.235724594745562 34.77984406210121 19.25209122536727 18.732340117785956 9 27.292856559411383 16.094575659899593 19.747035871142863 36.86553190954616 10-11 25.208211202658067 27.14370358641637 26.60758526527568 21.040499945649884 12-13 26.358391563354328 24.47043196716625 27.626194367317545 21.544982102161878 14-15 22.597709252651374 25.58677702567273 26.50325189612782 25.31226182554807 16-17 21.43675311790582 28.95569868799453 27.39688715219854 22.210661041901112 18-19 21.573288144198077 26.538406680854244 30.83112320008445 21.05718197486323 20-21 22.03912831213679 25.72302930570714 31.197311020255313 21.040531361900758 22-23 21.711393983033968 25.897892158063957 31.139662199904116 21.25105165899796 24-25 21.482716045504098 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29.109544068517586 21.831466893868992 50-51 21.858139290859818 27.633168775011264 28.985292796316507 21.52339913781241 52-53 22.266833298461293 27.344516261993938 28.852684801383322 21.535965638161443 54-55 21.46801228752404 27.61818322334504 29.142342634428566 21.771461854702352 56-57 21.73341677489565 27.86747117401901 28.669810805054 21.729301246031344 58-59 21.600494617453737 27.351145090928053 28.871723049412108 22.176637242206098 60-61 21.30973721562795 27.646143686621638 29.20102819105856 21.84309090669185 62-63 21.542280304586836 27.56421010434594 29.253399081263158 21.64011050980407 64-65 21.143143479633626 27.578261832541905 29.07273335605776 22.20586133176671 66-67 21.04528178354947 27.798969917494126 28.953524391416273 22.202223907540137 68-69 21.708355887788926 27.281900901560363 29.025864292498017 21.983878918152694 70-71 21.41106135124479 27.48373030396803 28.691622696991736 22.413585647795443 72-73 21.398319794487257 27.629519114136293 28.609895562858796 22.362265528517657 74-75 21.39606907302582 28.00551418035316 28.212484441101754 22.385932305519272 76-77 21.33791759266066 27.539768261167062 28.917182364424743 22.205131781747536 78-79 21.40935814714492 27.67372715488777 28.761389176347585 22.15552552161972 80-81 21.446204943541787 27.93096710023242 28.440507913449377 22.18232004277642 82-83 21.4567229075395 27.952504449369865 28.284891620733042 22.305881022357593 84-85 21.286319933997174 27.896279298022492 28.306660542261223 22.510740225719108 86-87 21.481756558063083 27.821908718698868 28.06878645986493 22.62754826337312 88-89 21.300783584129267 28.004226114067382 27.735428671601532 22.959561630201826 90-91 21.167995119111463 27.87346761431807 28.14264534985094 22.815891916719526 92-93 21.30536981488207 27.566813234593717 28.186658590560253 22.94115835996396 94-95 21.10456431144196 27.27619448550375 28.30240664265417 23.316834560400117 96-97 21.29336934892333 26.94433605837893 28.197153310675933 23.5651412820218 98-99 21.005313744672588 27.23449655143814 27.875262404235414 23.884927299653853 100-101 21.264448342854557 26.832039820751323 28.248568046385497 23.65494379000862 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3113.0 1 2131.0 2 2016.0 3 2728.0 4 2066.0 5 1644.5 6 2505.5 7 4741.5 8 6871.5 9 6918.5 10 5056.0 11 3479.0 12 3072.0 13 3031.5 14 3094.0 15 3114.0 16 3174.5 17 3376.0 18 3489.0 19 3652.5 20 4070.0 21 4609.0 22 5417.5 23 6507.0 24 7618.0 25 8971.0 26 10909.5 27 13211.0 28 15317.0 29 17305.5 30 19842.0 31 23675.5 32 28998.0 33 30611.0 34 29616.5 35 30858.0 36 33772.5 37 36882.0 38 39243.0 39 41075.5 40 42654.0 41 45686.0 42 48127.0 43 49590.0 44 51150.0 45 53992.5 46 55954.5 47 56731.0 48 57797.5 49 56567.0 50 54817.0 51 53041.0 52 51246.0 53 51322.5 54 51399.0 55 48392.5 56 44036.5 57 40038.0 58 36595.0 59 32476.5 60 26991.5 61 22754.0 62 19703.0 63 15450.0 64 11189.5 65 8508.5 66 6653.0 67 5385.0 68 4226.0 69 3131.5 70 2334.0 71 1715.0 72 1313.0 73 1136.5 74 958.0 75 702.5 76 494.0 77 349.0 78 248.5 79 170.5 80 107.5 81 62.5 82 46.0 83 28.0 84 12.0 85 7.0 86 3.5 87 5.0 88 3.0 89 1.0 90 1.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02086039057939735 2 0.010178865282717983 3 0.001445147540138973 4 0.0 5 6.283250174517274E-5 6 0.0 7 0.0 8 1.2566500349034547E-4 9 1.8849750523551823E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017593100488648367 26-27 0.00848238773559832 28-29 1.2566500349034547E-4 30-31 0.0 32-33 1.8849750523551823E-4 34-35 0.008827966495196769 36-37 9.739037770501775E-4 38-39 0.01140409906674885 40-41 0.010618692794934192 42-43 0.004146945115181401 44-45 6.597412683243138E-4 46-47 1.2566500349034547E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 3.141625087258637E-5 66-67 6.283250174517273E-4 68-69 9.110712753050045E-4 70-71 3.141625087258637E-5 72-73 9.424875261775911E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.2566500349034547E-4 82-83 9.739037770501775E-4 84-85 0.005403595150084855 86-87 0.0035814525994748462 88-89 0.0 90-91 0.0012252337840308685 92-93 4.398275122162092E-4 94-95 3.141625087258637E-5 96-97 0.0 98-99 0.0 100-101 6.283250174517274E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1591533.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.59709662977391 #Duplication Level Percentage of deduplicated Percentage of total 1 75.83146369641145 39.88514823617344 2 13.423470943001337 14.120711965920071 3 4.568688468921204 7.208992466135471 4 2.054959820286394 4.323396809516252 5 1.113393620987905 2.9280635935037354 6 0.6543453923966998 2.0650000699881925 7 0.43976201569283097 1.6191143663449985 8 0.3065258886379121 1.2897897427372462 9 0.22622179648292914 1.0708748720436303 >10 1.2438159506487154 11.906575668780954 >50 0.08717062324929074 3.124934249151036 >100 0.04227743329536088 4.182722070093696 >500 0.004311463629774429 1.5589209918191618 >1k 0.0032335977223308223 2.9269138469725777 >5k 2.3952575720969052E-4 1.0316166592460974 >10k+ 1.1976287860484526E-4 0.7572243915733855 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12021 0.7553095034787215 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8901 0.5592720980337825 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7476 0.4697357830469114 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4311 0.2708709150234397 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2774 0.17429735984110917 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2707 0.1700875822241826 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2554 0.16047420945717117 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2430 0.15268297924076976 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2186 0.13735184881494758 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2138 0.1343358887311793 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1948 0.1223977133995965 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1901 0.11944458581757338 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 1854 0.11649145823555025 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1774 0.11146485809593644 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1670 0.10493027791443846 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 3.141625087258637E-5 14-15 0.0 0.0 0.0 0.0 6.283250174517274E-5 16-17 0.0 0.0 0.0 3.141625087258637E-5 6.283250174517274E-5 18-19 0.0 0.0 0.0 6.283250174517274E-5 6.283250174517274E-5 20-21 0.0 0.0 0.0 1.8849750523551823E-4 6.283250174517274E-5 22-23 0.0 0.0 0.0 5.340762648339682E-4 6.283250174517274E-5 24-25 0.0 0.0 0.0 0.0011624012822856957 6.283250174517274E-5 26-27 0.0 0.0 0.0 0.0021363050593358733 6.283250174517274E-5 28-29 0.0 0.0 0.0 0.004523940125652437 6.283250174517274E-5 30-31 0.0 0.0 0.0 0.01470280540837042 6.283250174517274E-5 32-33 0.0 0.0 0.0 0.03248440340225431 6.283250174517274E-5 34-35 0.0 0.0 0.0 0.06028778542449324 6.283250174517274E-5 36-37 0.0 0.0 0.0 0.10672100421417588 6.283250174517274E-5 38-39 0.0 0.0 0.0 0.18080052377173456 9.424875261775911E-5 40-41 0.0 0.0 0.0 0.2654987361242274 1.2566500349034547E-4 42-43 0.0 0.0 0.0 0.35289874605176264 1.2566500349034547E-4 44-45 0.0 0.0 0.0 0.45830026772928995 1.2566500349034547E-4 46-47 0.0 0.0 0.0 0.5811692248919753 1.2566500349034547E-4 48-49 0.0 0.0 0.0 0.7177670836859807 1.2566500349034547E-4 50-51 0.0 0.0 0.0 0.8753195818120014 1.2566500349034547E-4 52-53 0.0 0.0 0.0 1.022002057136107 1.2566500349034547E-4 54-55 0.0 0.0 0.0 1.1792718090042746 1.2566500349034547E-4 56-57 0.0 0.0 0.0 1.3651303491664954 1.5708125436293184E-4 58-59 0.0 0.0 0.0 1.5711581223889168 1.884975052355182E-4 60-61 0.0 0.0 0.0 1.8055547701492838 1.884975052355182E-4 62-63 0.0 0.0 0.0 2.0297725526269326 1.884975052355182E-4 64-65 0.0 0.0 0.0 2.2555297313973384 2.199137561081046E-4 66-67 0.0 0.0 0.0 2.514619552343558 2.5133000698069095E-4 68-69 0.0 0.0 0.0 2.7976171402038164 2.5133000698069095E-4 70-71 0.0 0.0 0.0 3.109863257626452 2.5133000698069095E-4 72-73 0.0 0.0 0.0 3.4304975140320684 3.1416250872586367E-4 74-75 0.0 0.0 0.0 3.747016241573376 3.1416250872586367E-4 76-77 0.0 0.0 0.0 4.0840811971853554 3.1416250872586367E-4 78-79 0.0 0.0 0.0 4.465977142792514 3.1416250872586367E-4 80-81 0.0 0.0 0.0 4.876399044191983 3.1416250872586367E-4 82-83 0.0 0.0 0.0 5.301303837243714 3.1416250872586367E-4 84-85 0.0 0.0 0.0 5.713673546197283 3.1416250872586367E-4 86-87 0.0 0.0 0.0 6.139929238036535 3.1416250872586367E-4 88-89 0.0 0.0 0.0 6.574792982614875 3.1416250872586367E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4795 0.0 48.056843 1 GTATCAA 8910 0.0 43.032627 1 TATCAAC 11445 0.0 33.28627 2 ATCAACG 11680 0.0 32.533173 3 TCAACGC 12020 0.0 31.651451 4 CAACGCA 12230 0.0 31.069132 5 TGTACCG 125 2.1560118E-7 30.39804 5 AACGCAG 12645 0.0 30.124588 6 GTACCGT 130 3.0398405E-7 29.228882 6 GTACATG 9005 0.0 27.22501 1 TACATGG 9010 0.0 26.834873 2 ACGCAGA 14160 0.0 26.76734 7 CGCAGAG 14180 0.0 26.696093 8 TATAACG 110 9.4769544E-5 25.909863 2 TACCGTA 110 9.483029E-5 25.907421 7 ACATGGG 9035 0.0 25.339506 3 GCAGAGT 14920 0.0 24.894506 9 GAGTACT 9425 0.0 22.954754 12-13 CATGGGA 5720 0.0 22.668995 4 AGTACTT 9630 0.0 22.589405 12-13 >>END_MODULE