FastQCFastQC Report
Thu 26 May 2016
SRR938311_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938311_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2823336
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100280.35518266334577253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77240.2735770733628587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56260.19926781651209777No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA43410.15375428216832854No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA41010.14525369987844167No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40530.14355358342046431No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA35790.12676493339793776No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC34010.12046033486627168No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA32260.11426199361322918No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG29550.10466341944423194No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47800.055.519921
GTATCAA87000.043.5147361
ATCAACG114300.032.8301853
TATCAAC115500.032.5714452
TCAACGC116350.032.2108654
CAACGCA117850.031.8008825
AACGCAG122700.030.5555346
TGGTATC19050.030.2087632
GTGGTAT22650.027.717131
ACGCAGA140700.026.4424697
CGCAGAG140750.026.2638158
TACATGG90500.025.6982082
GTACATG96950.025.1168211
ACATGGG93350.023.8946723
TAAGGTG20200.022.8382555
GCAGAGT156800.022.8174929
AGGTAAG17000.022.1014482
GTAAGGT19050.021.720354
GAGTACT104550.021.4042712-13
GTGTAAG13250.021.1776961
Produced by FastQC (version 0.11.5)