FastQCFastQC Report
Thu 26 May 2016
SRR938311_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938311_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2823336
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95120.33690641142251576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82750.29309299353672397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61010.21609188562749884No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40330.14284520156297373No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC38780.1373552421674218No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36920.13076729089275949No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA35150.12449811145396793No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA33420.11837060838667449No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG32030.113447354477115No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48900.057.056431
GTATCAA87950.042.6939771
ATCAACG116200.031.9724483
TATCAAC116950.031.7814832
TCAACGC118250.031.4181674
CAACGCA120200.030.9479965
GTGGTAT21300.029.4557231
AACGCAG126400.029.340776
TGGTATC20200.028.470932
ACGCAGA144100.025.637947
CGCAGAG144950.025.5203578
TACATGG85850.023.3082392
GCAGAGT163200.022.2009689
GTACATG94050.022.0344921
TAAGGTG20950.021.9969775
GTAAGGT20900.021.8222834
ACATGGG89350.021.5877173
AGGTAAG19700.020.9903982
GAGTACT109550.020.76302712-13
AGAGTAC149900.020.62276510-11
Produced by FastQC (version 0.11.5)