##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938311_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2823336 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.632281811304075 31.0 31.0 34.0 30.0 34.0 2 31.87858299543519 31.0 31.0 34.0 30.0 34.0 3 31.930501364343456 31.0 31.0 34.0 30.0 34.0 4 35.639844496014646 37.0 35.0 37.0 33.0 37.0 5 35.53617670727112 37.0 35.0 37.0 33.0 37.0 6 35.53439371013581 37.0 35.0 37.0 33.0 37.0 7 35.472848785975174 37.0 35.0 37.0 33.0 37.0 8 35.44375306375154 37.0 35.0 37.0 33.0 37.0 9 36.99859634134938 39.0 37.0 39.0 33.0 39.0 10-11 36.89008286650969 39.0 37.0 39.0 33.0 39.0 12-13 36.77291562180342 39.0 37.0 39.0 32.0 39.0 14-15 37.747004076029214 39.5 37.0 41.0 32.0 41.0 16-17 37.64339827069821 39.0 36.5 41.0 32.0 41.0 18-19 37.90884790191461 40.0 37.0 41.0 33.0 41.0 20-21 37.88134231986558 40.0 37.0 41.0 32.0 41.0 22-23 37.761467285509056 40.0 37.0 41.0 32.0 41.0 24-25 37.62036204688354 39.0 37.0 41.0 32.0 41.0 26-27 37.39989625747697 39.0 36.0 41.0 32.0 41.0 28-29 37.272221230487624 39.0 36.0 41.0 31.5 41.0 30-31 36.978215132736594 39.0 36.0 40.0 31.0 41.0 32-33 36.74307875506139 39.0 35.0 40.0 30.0 41.0 34-35 36.60761365278522 39.0 35.0 40.0 30.0 41.0 36-37 36.527545428528526 39.0 35.0 40.0 30.0 41.0 38-39 36.32837855643112 38.5 35.0 40.0 30.0 41.0 40-41 36.127576916102086 38.0 35.0 40.0 30.0 41.0 42-43 35.88059479991046 38.0 35.0 40.0 29.0 41.0 44-45 35.551362997531996 38.0 34.5 40.0 27.5 41.0 46-47 35.12711292598543 38.0 34.0 40.0 26.5 41.0 48-49 34.717103809110924 38.0 33.5 40.0 25.5 41.0 50-51 34.29917232663771 38.0 33.0 40.0 24.0 41.0 52-53 33.751130223253625 37.0 33.0 40.0 23.0 41.0 54-55 33.36847013603765 37.0 32.0 40.0 22.0 41.0 56-57 32.9927148238821 36.5 31.0 39.0 20.5 40.5 58-59 32.46243114528345 36.0 31.0 39.0 18.5 40.0 60-61 32.024998441559916 35.5 30.5 39.0 17.5 40.0 62-63 31.497048526990767 35.0 29.5 38.5 13.5 40.0 64-65 30.921504737657862 34.5 29.0 38.0 8.5 40.0 66-67 30.37462933919307 34.0 28.0 38.0 8.0 40.0 68-69 29.812975147130913 34.0 28.0 37.0 7.0 39.0 70-71 29.160912657933736 34.0 26.5 36.5 2.0 39.0 72-73 28.598235916660293 33.0 26.0 36.0 2.0 39.0 74-75 27.95732388918641 32.5 26.0 35.5 2.0 38.0 76-77 26.847992233301316 31.0 25.0 34.0 2.0 36.0 78-79 27.507976379715345 32.0 26.0 35.0 2.0 37.0 80-81 27.071920239036373 32.0 25.0 35.0 2.0 36.5 82-83 26.543026936928513 32.0 24.5 35.0 2.0 36.0 84-85 25.88403133739661 31.5 22.5 34.0 2.0 35.5 86-87 25.169967549027106 31.0 20.0 34.0 2.0 35.0 88-89 24.287440460504875 30.5 17.5 34.0 2.0 35.0 90-91 23.54730078177022 30.0 9.0 34.0 2.0 35.0 92-93 22.59172323095799 29.0 2.0 34.0 2.0 35.0 94-95 21.623067534292765 29.0 2.0 34.0 2.0 35.0 96-97 20.396499035183908 28.0 2.0 33.5 2.0 35.0 98-99 18.877718946664515 26.0 2.0 33.0 2.0 34.0 100-101 15.188937660979777 11.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2521304580423305 1101 2 0.30580843614413666 1101 3 0.3446805081468902 1101 4 0.22125227945328874 1101 5 0.1348451142050635 1101 6 0.23955135206562517 1101 7 0.20249160725943227 1101 8 0.2049137537369745 1101 9 0.3606493506856623 1101 10-11 0.30759371704597527 1101 12-13 0.367716359768842 1101 14-15 0.4655445727471488 1101 16-17 0.419642205950737 1101 18-19 0.504243434653695 1101 20-21 0.49021559466945064 1101 22-23 0.5093195384507325 1101 24-25 0.5786560974770154 1101 26-27 0.4824205124709593 1101 28-29 0.45789790518410456 1101 30-31 0.5033238330124661 1101 32-33 0.30416072625206425 1101 34-35 0.4261179102766519 1101 36-37 0.6285734844575614 1101 38-39 0.6018784417509409 1101 40-41 0.5893103253201204 1101 42-43 0.6152053452698922 1101 44-45 0.5607598852410618 1101 46-47 0.4478271295632936 1101 48-49 0.5430707785606614 1101 50-51 0.5422296258667316 1101 52-53 0.6070527954867231 1101 54-55 0.4630717444727992 1101 56-57 0.45984889657492545 1101 58-59 0.6254896033772255 1101 60-61 0.6537962733859217 1101 62-63 0.7955539367336328 1101 64-65 0.6022686080729649 1101 66-67 0.4446508734834502 1101 68-69 0.6543222916078228 1101 70-71 0.7283785899302444 1101 72-73 0.8649856766223962 1101 74-75 0.9110961218529461 1101 76-77 0.8205402019849721 1101 78-79 0.9652054111769743 1101 80-81 1.0128637127399962 1101 82-83 1.0819629560122834 1101 84-85 1.1598925265348292 1101 86-87 1.19040100266864 1101 88-89 1.2303226330188046 1101 90-91 1.2536239513706455 1101 92-93 1.3976325546495225 1101 94-95 1.423970493689005 1101 96-97 1.5147528658976412 1101 98-99 1.5662168438947006 1101 100-101 1.9466159964828638 1102 1 0.34505966122260645 1102 2 0.35435896019727053 1102 3 0.2799901424241362 1102 4 0.25758457960285597 1102 5 0.1291145705058696 1102 6 0.21160830665300523 1102 7 0.20106086987151173 1102 8 0.2344857437871255 1102 9 0.2095425226737433 1102 10-11 0.33671404517390613 1102 12-13 0.40708821745299417 1102 14-15 0.5433686368538986 1102 16-17 0.5143567932069288 1102 18-19 0.4338424598689201 1102 20-21 0.42837094586649727 1102 22-23 0.3700580617004121 1102 24-25 0.5380661385347665 1102 26-27 0.41945914427896724 1102 28-29 0.4907484785612226 1102 30-31 0.4044009489223157 1102 32-33 0.5431791143411004 1102 34-35 0.5152004878973813 1102 36-37 0.6172857334757254 1102 38-39 0.6263500124902279 1102 40-41 0.513735647701175 1102 42-43 0.4481322018585914 1102 44-45 0.5694022149432598 1102 46-47 0.6052439347282217 1102 48-49 0.5606341431780848 1102 50-51 0.7014285875948829 1102 52-53 0.7913380741938383 1102 54-55 0.7223873902772056 1102 56-57 0.6729435230700744 1102 58-59 0.7573378631973142 1102 60-61 0.6485392247829935 1102 62-63 0.9844513310677172 1102 64-65 0.9119326923497759 1102 66-67 0.6803044846714492 1102 68-69 0.9050417421560653 1102 70-71 0.8712026711238323 1102 72-73 0.9831496623651432 1102 74-75 0.9959367985370484 1102 76-77 0.9703239396497025 1102 78-79 1.1173262477330752 1102 80-81 1.2045777763797112 1102 82-83 1.2208751019778745 1102 84-85 1.15355559015822 1102 86-87 1.011067677688871 1102 88-89 1.1155957584319722 1102 90-91 1.0554981490014974 1102 92-93 1.4115877499387182 1102 94-95 1.393684274067013 1102 96-97 1.4096540413577614 1102 98-99 1.4384477804011695 1102 100-101 1.5097074144506966 1103 1 0.3232865910383147 1103 2 0.23823006501696042 1103 3 0.28439466861118845 1103 4 0.18798120188652234 1103 5 0.09679068093453935 1103 6 0.15102596057070627 1103 7 0.2350253266236848 1103 8 0.12458223487944764 1103 9 0.23114230042711625 1103 10-11 0.3417273669527674 1103 12-13 0.3917784268359341 1103 14-15 0.5627959342313247 1103 16-17 0.4484114086930049 1103 18-19 0.45880682500087033 1103 20-21 0.43126551897638166 1103 22-23 0.44017812351091123 1103 24-25 0.45408139269004266 1103 26-27 0.4319336201829671 1103 28-29 0.42940535291598536 1103 30-31 0.4526818659008711 1103 32-33 0.37744437040001344 1103 34-35 0.4441139922535129 1103 36-37 0.5816246443444726 1103 38-39 0.5382707263518824 1103 40-41 0.6672926989836938 1103 42-43 0.6968484132119812 1103 44-45 0.7741786627947604 1103 46-47 0.7017454663582043 1103 48-49 0.7702531994994786 1103 50-51 0.9694316569956172 1103 52-53 0.8698297815799236 1103 54-55 0.8128527326507253 1103 56-57 0.8630627595615437 1103 58-59 0.8585779787124963 1103 60-61 0.8346842800044527 1103 62-63 0.7799304619183545 1103 64-65 0.8370716517551351 1103 66-67 0.8888926291111225 1103 68-69 0.7132748766022523 1103 70-71 0.6956522189546277 1103 72-73 1.123024348971125 1103 74-75 1.0627752961322052 1103 76-77 0.89374342302456 1103 78-79 1.181112600149703 1103 80-81 1.2492766614375803 1103 82-83 1.36106972251903 1103 84-85 1.4948816223658028 1103 86-87 1.2726072832993687 1103 88-89 1.3317862332188461 1103 90-91 1.6155574860001316 1103 92-93 1.785469669876388 1103 94-95 1.5031536307824211 1103 96-97 1.656385864013405 1103 98-99 1.80789041741334 1103 100-101 1.9456601408650531 1104 1 0.24664356241746788 1104 2 0.25907310708892695 1104 3 0.3079188727649189 1104 4 0.24752198431267658 1104 5 0.1880424893237489 1104 6 0.18620208205820887 1104 7 0.25860128421734885 1104 8 0.2608891094057384 1104 9 0.3030752890237167 1104 10-11 0.3339292921807342 1104 12-13 0.36150218748414886 1104 14-15 0.4818878974706422 1104 16-17 0.4926212481428678 1104 18-19 0.5133922751770896 1104 20-21 0.4476142398555325 1104 22-23 0.4148898902397846 1104 24-25 0.4995900765356751 1104 26-27 0.41250640436062724 1104 28-29 0.508707482820455 1104 30-31 0.454390206408938 1104 32-33 0.4954054852024967 1104 34-35 0.5849218658358453 1104 36-37 0.615715016821369 1104 38-39 0.5245455709842375 1104 40-41 0.7526014476045972 1104 42-43 0.8249914807075314 1104 44-45 0.7775100818073 1104 46-47 0.7788083656288407 1104 48-49 0.7633381528903769 1104 50-51 1.0366401766135098 1104 52-53 1.053626041881131 1104 54-55 1.2536349918577088 1104 56-57 1.1890056857725924 1104 58-59 1.2516386771502113 1104 60-61 1.256663584352367 1104 62-63 1.1767503203572005 1104 64-65 1.1313872637884508 1104 66-67 1.2147085566734752 1104 68-69 1.4416606605762396 1104 70-71 1.3643900169483132 1104 72-73 1.4301745756130302 1104 74-75 1.6165796748071166 1104 76-77 1.4696639306948 1104 78-79 1.582589314202167 1104 80-81 1.6164929559962182 1104 82-83 1.5584049927506918 1104 84-85 1.749460370829297 1104 86-87 1.87191748387853 1104 88-89 1.9970197032416763 1104 90-91 1.9988140080067893 1104 92-93 2.1776010720988737 1104 94-95 2.1876053949069387 1104 96-97 2.4029396096030347 1104 98-99 2.681953191235543 1104 100-101 2.5920374716181858 1105 1 0.23957123503222633 1105 2 0.2912109708217052 1105 3 0.2620938819396592 1105 4 0.21332582197005934 1105 5 0.18544522495222537 1105 6 0.2662459273088018 1105 7 0.2661246887393176 1105 8 0.3084336302353421 1105 9 0.3198630530841058 1105 10-11 0.2877135828550266 1105 12-13 0.398977528562277 1105 14-15 0.49520561588654743 1105 16-17 0.42414535439156964 1105 18-19 0.4848708613382371 1105 20-21 0.3738279955887336 1105 22-23 0.46483360926711015 1105 24-25 0.4325220134189678 1105 26-27 0.36231364836456237 1105 28-29 0.2917935185274274 1105 30-31 0.47668961242926855 1105 32-33 0.5511808967067182 1105 34-35 0.5410519073658051 1105 36-37 0.49774694193243363 1105 38-39 0.3827930257273522 1105 40-41 0.5391270616673367 1105 42-43 0.5469782197769177 1105 44-45 0.6082704554871654 1105 46-47 0.5053551190368566 1105 48-49 0.6722188434484622 1105 50-51 0.8028737700649202 1105 52-53 0.721653024034552 1105 54-55 0.6909682136888335 1105 56-57 0.6954389488180652 1105 58-59 0.7273604524036799 1105 60-61 0.7366074116566281 1105 62-63 0.6228839811015803 1105 64-65 0.7543389001640683 1105 66-67 0.534548048776287 1105 68-69 0.7679593906720008 1105 70-71 0.5941069234253717 1105 72-73 0.6356065188316791 1105 74-75 0.7434521459933556 1105 76-77 0.6938837665239177 1105 78-79 0.8737977332643467 1105 80-81 0.8666200086218083 1105 82-83 0.8960556964010742 1105 84-85 1.0862120302886353 1105 86-87 1.0738332485637052 1105 88-89 1.2914075480259903 1105 90-91 1.3474009132697624 1105 92-93 1.5503642029243743 1105 94-95 1.6126682473685463 1105 96-97 1.7746396681877705 1105 98-99 1.609619297856998 1105 100-101 1.62752345424642 1106 1 0.06140774560270401 1106 2 0.10493776874306349 1106 3 0.13772064993511535 1106 4 0.0359703780242242 1106 5 -0.005737414982476707 1106 6 -0.08481637317077428 1106 7 0.02859444894735219 1106 8 0.13204427003458363 1106 9 -0.14600194952721068 1106 10-11 0.1762054370884556 1106 12-13 0.09167990450396957 1106 14-15 0.09022692686230016 1106 16-17 0.19067319619480827 1106 18-19 0.11694661557353214 1106 20-21 0.16343788988969976 1106 22-23 0.18693363596774049 1106 24-25 0.131510787403748 1106 26-27 0.22231501680082033 1106 28-29 0.2901497247890106 1106 30-31 0.3537408557595825 1106 32-33 0.14236379005554056 1106 34-35 0.02682207089393529 1106 36-37 0.2401169306969706 1106 38-39 0.03377318547501318 1106 40-41 0.14887622545832357 1106 42-43 0.23903796054348447 1106 44-45 0.07108492801373245 1106 46-47 -0.5451834320471676 1106 48-49 -0.39541099270087443 1106 50-51 0.04245015610769798 1106 52-53 0.12932665705542945 1106 54-55 0.39444155371690215 1106 56-57 0.21070766800089302 1106 58-59 -0.032195909315952065 1106 60-61 0.25768887485133973 1106 62-63 -0.005580506710472832 1106 64-65 -0.036760378043400266 1106 66-67 -0.05237961830943405 1106 68-69 -0.25041042623168863 1106 70-71 -0.14483759754245895 1106 72-73 0.155567603637639 1106 74-75 0.19603968847765785 1106 76-77 0.25879585140566874 1106 78-79 0.5253439280094128 1106 80-81 0.07069664704201628 1106 82-83 0.5153427917937528 1106 84-85 0.6110461534677114 1106 86-87 1.0122114004882334 1106 88-89 1.004367618484327 1106 90-91 0.8407483894148555 1106 92-93 1.1154001151610764 1106 94-95 1.4314536519130883 1106 96-97 1.5297338679762404 1106 98-99 1.5423428016251535 1106 100-101 1.4505473827596784 1107 1 0.1314824903993177 1107 2 0.13655908890661195 1107 3 0.12943048266206247 1107 4 0.12338733107657163 1107 5 -0.003251009210728739 1107 6 0.12208824440118349 1107 7 0.13249677083634026 1107 8 0.11353319217152347 1107 9 0.19694183823438038 1107 10-11 0.221596728597234 1107 12-13 0.2726618793339881 1107 14-15 0.30741113026618905 1107 16-17 0.26704744610702846 1107 18-19 0.30283633913061436 1107 20-21 0.33423060936620175 1107 22-23 0.23687864629978606 1107 24-25 0.32422622785583854 1107 26-27 0.26682714064334334 1107 28-29 0.32355283907639887 1107 30-31 0.22342715688816384 1107 32-33 0.2545819425700202 1107 34-35 0.36252342318303477 1107 36-37 0.32718646752885405 1107 38-39 0.3381434282865001 1107 40-41 0.27893843478128844 1107 42-43 0.3138134514314146 1107 44-45 0.3136476997824147 1107 46-47 0.2603396266445799 1107 48-49 0.3350279930743909 1107 50-51 0.31777151893798106 1107 52-53 0.31479446153188917 1107 54-55 0.08024128595882019 1107 56-57 0.17741557315004286 1107 58-59 0.1926942526661506 1107 60-61 0.3965775250697874 1107 62-63 0.40472867676757573 1107 64-65 0.43736547955913707 1107 66-67 0.3794454598281227 1107 68-69 0.5965217517458079 1107 70-71 0.789850074936183 1107 72-73 0.8712451308390996 1107 74-75 0.7453547596035826 1107 76-77 0.833720348013923 1107 78-79 0.9363567056456219 1107 80-81 0.84928799580155 1107 82-83 0.934566089866923 1107 84-85 1.1519380488001225 1107 86-87 1.3650365399012898 1107 88-89 1.295245721177988 1107 90-91 1.325060449901681 1107 92-93 1.376017733696937 1107 94-95 1.5163707457181843 1107 96-97 1.6270378254008513 1107 98-99 1.8102732885346953 1107 100-101 1.7452637959319635 1108 1 0.13024405580552667 1108 2 0.10253784990328896 1108 3 0.06391763699951625 1108 4 0.054358126502812354 1108 5 0.01655961215708146 1108 6 0.12897333646566977 1108 7 0.11500139677666255 1108 8 0.10786742888414835 1108 9 0.17095259569781263 1108 10-11 0.1638254043397609 1108 12-13 0.24658093998539954 1108 14-15 0.42949318886017807 1108 16-17 0.30787018922687537 1108 18-19 0.1914590918922343 1108 20-21 0.3265513484858289 1108 22-23 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-2.1069291278164037 2316 90-91 -2.0196516941781617 2316 92-93 -2.510935549322749 2316 94-95 -2.4333883339314752 2316 96-97 -2.462832986574078 2316 98-99 -2.1614133706429115 2316 100-101 -1.9556730861172102 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 24.0 4 54.0 5 122.0 6 245.0 7 710.0 8 1657.0 9 2940.0 10 4768.0 11 7278.0 12 10262.0 13 14220.0 14 18784.0 15 24662.0 16 30445.0 17 36134.0 18 35271.0 19 31762.0 20 32167.0 21 33891.0 22 36818.0 23 41278.0 24 47118.0 25 54639.0 26 64180.0 27 75450.0 28 89246.0 29 104176.0 30 122467.0 31 142969.0 32 169548.0 33 203060.0 34 250647.0 35 307827.0 36 364691.0 37 339466.0 38 121484.0 39 2871.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.124864787134044 20.2055306401032 15.795908796929577 22.873695775833184 2 15.911771991179183 21.132007139715963 39.19037151824565 23.76584935085921 3 19.27946230983489 24.21380239546409 30.57585069577266 25.930884598928362 4 13.334828019052638 17.695201704650103 36.78527812488489 32.18469215141237 5 13.394117597033711 37.72405205746618 34.62516725062648 14.256663094873625 6 32.10649816617582 37.17628265862889 16.877345408887006 13.839873766308283 7 28.315545864891746 32.92923690272784 20.476698487179707 18.278518745200714 8 26.343658707288114 35.65289430659334 19.442177622500477 18.561269363618074 9 25.978310764287354 16.719901563257082 20.002118061753897 37.29966961070167 10-11 24.76435677510576 26.965564849525524 27.493043690159443 20.77703468520927 12-13 25.86089647140829 24.073631335413143 27.77747671548834 22.287995477690224 14-15 22.126838605111114 25.767974481251965 26.202602169915306 25.90258474372161 16-17 21.688810683531823 28.87033282613192 27.28520445317171 22.155652037164547 18-19 21.727010175197 27.116946052471263 29.746494926569138 21.4095488457626 20-21 22.123721724938157 26.333688232643937 30.112002255487834 21.430587786930072 22-23 21.80202781390525 26.38823363567071 30.045520618162346 21.764217932261694 24-25 21.815253120616614 26.54226115416767 30.04541010782244 21.597075617393273 26-27 21.360618599204756 26.962928251588046 29.76914810493603 21.907305044271173 28-29 21.553125330947502 27.29484973965168 29.672181779478368 21.479843149922452 30-31 21.69136711134787 26.793843082694835 29.563549128394612 21.951240677562676 32-33 21.317992550624727 26.792213716881236 29.636998799996032 22.252794932498006 34-35 22.164006178512683 27.03161289397007 29.307056883797237 21.49732404372001 36-37 22.245348318571402 26.49131732188704 29.41477080896974 21.84856355057181 38-39 21.667742566770006 26.894474123938377 29.368176557420746 22.06960675187087 40-41 22.218705852913086 26.703399050084414 29.411237580373488 21.666657516629012 42-43 21.920779826132293 26.730636815976787 29.178148518610868 22.17043483928005 44-45 21.957120715919295 26.97195983934747 29.33259986366107 21.73831958107216 46-47 22.387470000028337 27.25141463856941 28.46209236166011 21.899022999742147 48-49 22.05061671724513 27.399218513134816 28.352966136513686 22.197198633106368 50-51 22.30353503842903 27.48232888107146 28.35263359132143 21.861502489178083 52-53 22.64321356012887 27.141703998390554 28.345209355174163 21.869873086306413 54-55 22.049091488683455 27.39976268905182 28.540643927319092 22.01050189494563 56-57 22.282611775573294 27.583521762907427 28.109406744361987 22.024459717157292 58-59 22.091863630502033 27.203806731465512 28.222092873307815 22.48223676472464 60-61 21.72100663895477 27.459714323764512 28.5021513557012 22.31712768157952 62-63 21.930654972489933 27.403072732312395 28.633458581697326 22.032813713500346 64-65 21.631268106367134 27.465937535203132 28.41398674130381 22.48880761712592 66-67 21.54087891586291 27.584698431556117 28.37700531870711 22.497417333873866 68-69 22.194647730306 27.09944547344474 28.46384259076394 22.242064205485327 70-71 21.966897652403567 27.253642811363655 28.094085203152513 22.68537433308026 72-73 21.879026710973623 27.33413758343859 28.138080723003373 22.64875498258442 74-75 21.946109407277167 27.681625411526973 27.82598976377032 22.54627541742554 76-77 21.92194065091154 27.314677171816538 28.199817787575466 22.56356438969646 78-79 21.926080819475583 27.57076380838466 28.139977920189562 22.363177451950193 80-81 22.173049748910977 27.436982404017286 27.90229732858429 22.487670518487445 82-83 22.09059413693431 27.605793194739412 27.948398247415447 22.355214420910833 84-85 21.95043325425926 27.722905734054276 27.865154050977363 22.461506960709105 86-87 22.154490084035043 27.64842686903891 27.57256915706549 22.624513889860552 88-89 21.95894414638599 27.710840170363007 27.45114824878103 22.879067434469974 90-91 21.843678961508818 27.639188722163187 27.643227503676975 22.873904812651023 92-93 21.983274992963196 27.3316530031341 27.769059640385247 22.916012363517453 94-95 21.718064319443517 27.0961691655889 27.93981116944813 23.24595534551945 96-97 21.894149559158755 26.77367137746523 27.99034962757875 23.341829435797262 98-99 21.57571668305945 27.08528719326872 27.56525235114356 23.773743772528267 100-101 21.73113151069065 26.764848204031694 28.087762590269556 23.416257695008103 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 678.0 1 459.5 2 356.0 3 470.0 4 404.5 5 393.5 6 772.0 7 1815.5 8 2771.0 9 3046.0 10 2586.0 11 1994.0 12 1936.5 13 2029.5 14 2268.5 15 2589.0 16 2859.0 17 3246.5 18 3635.0 19 4052.0 20 4816.0 21 5805.5 22 7118.5 23 9004.0 24 11370.5 25 14385.5 26 18550.0 27 23851.0 28 28784.5 29 33445.0 30 38237.5 31 45176.5 32 55756.0 33 60316.5 34 60950.5 35 64823.5 36 69901.0 37 75279.0 38 78671.0 39 81478.0 40 85487.0 41 89938.0 42 92690.5 43 94363.0 44 96185.5 45 100036.5 46 102178.5 47 103663.0 48 104134.5 49 101222.0 50 98629.0 51 95305.5 52 92563.0 53 92346.0 54 93149.5 55 87511.0 56 78993.0 57 71256.0 58 64331.5 59 56006.5 60 44150.0 61 36229.0 62 30833.5 63 22785.5 64 15337.0 65 11231.0 66 8465.5 67 6679.5 68 5272.0 69 4190.5 70 3310.5 71 2327.5 72 1764.0 73 1442.0 74 1092.5 75 792.5 76 591.0 77 418.0 78 266.0 79 167.5 80 107.0 81 73.0 82 42.5 83 19.0 84 7.5 85 3.5 86 3.5 87 1.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.541909287452857E-4 2 0.048701252702476786 3 0.0 4 0.0 5 0.04335296967842297 6 3.541909287452857E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006233760345917028 26-27 0.001912631015224543 28-29 1.0625727862358572E-4 30-31 0.0014167637149811428 32-33 4.250291144943429E-4 34-35 0.01156433382353358 36-37 0.022916153089819986 38-39 0.004197162505631636 40-41 0.0011511205184221787 42-43 0.0014167637149811428 44-45 0.007951586350331665 46-47 0.0 48-49 0.0 50-51 5.135768466806642E-4 52-53 0.0 54-55 0.0012750873434830285 56-57 0.0 58-59 0.02309324855419263 60-61 0.0 62-63 0.009864217365556207 64-65 0.0153718863075454 66-67 0.006641079913974108 68-69 0.01691261684758739 70-71 5.312863931179286E-5 72-73 7.083818574905714E-5 74-75 0.021853580303584128 76-77 0.04799287084498621 78-79 0.03003539075760023 80-81 0.02590906643771765 82-83 0.03506490194578329 84-85 0.016133396804347763 86-87 0.010182989201426965 88-89 0.08149933270429024 90-91 0.02488191274435632 92-93 0.023961016329618577 94-95 0.026652867388082754 96-97 0.03759736708631208 98-99 0.05187126151474709 100-101 0.014238475335560486 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2823336.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.31908026617431 #Duplication Level Percentage of deduplicated Percentage of total 1 74.39520239479266 40.41078970301026 2 14.463977564043503 15.71339916538847 3 5.061847006031203 8.248646214471092 4 2.200401911962406 4.780952322949174 5 1.1563192594523475 3.1405099333757645 6 0.6937993828814124 2.261192662041459 7 0.42157109938769854 1.602954806987782 8 0.29287575927186393 1.2726993500724098 9 0.20084694703082331 0.9818839293285018 >10 0.9984206941392822 9.678308615049929 >50 0.06809440146700575 2.509013829897317 >100 0.04006423423174897 4.153075631891783 >500 0.003257101637785334 1.2126640178867587 >1k 0.003126817572080112 3.188643614214241 >5k 1.95426098255007E-4 0.8452662034350911 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9512 0.33690641142251576 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8275 0.29309299353672397 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6101 0.21609188562749884 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4033 0.14284520156297373 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3878 0.1373552421674218 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3692 0.13076729089275949 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3515 0.12449811145396793 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3342 0.11837060838667449 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3203 0.113447354477115 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 1.7709546437264286E-5 16-17 0.0 0.0 0.0 0.0 3.541909287452857E-5 18-19 0.0 0.0 0.0 0.0 3.541909287452857E-5 20-21 0.0 0.0 0.0 0.0 3.541909287452857E-5 22-23 0.0 0.0 0.0 8.854773218632144E-5 3.541909287452857E-5 24-25 0.0 0.0 0.0 1.7709546437264285E-4 3.541909287452857E-5 26-27 0.0 0.0 0.0 5.489959395551928E-4 3.541909287452857E-5 28-29 0.0 0.0 0.0 0.002089726479597186 3.541909287452857E-5 30-31 0.0 0.0 0.0 0.007916167257457135 3.541909287452857E-5 32-33 0.0 0.0 0.0 0.019799272916861472 3.541909287452857E-5 34-35 0.0 0.0 0.0 0.037508819354125755 3.541909287452857E-5 36-37 0.0 0.0 0.0 0.06890784518739534 3.541909287452857E-5 38-39 0.0 0.0 0.0 0.11670591102157164 3.541909287452857E-5 40-41 0.0 0.0 0.0 0.16928555439380932 3.541909287452857E-5 42-43 0.0 0.0 0.0 0.2223964841591649 3.541909287452857E-5 44-45 0.0 0.0 0.0 0.2930929935367239 3.541909287452857E-5 46-47 0.0 0.0 0.0 0.38151675889798453 3.541909287452857E-5 48-49 0.0 0.0 0.0 0.47165835026365976 3.541909287452857E-5 50-51 0.0 0.0 0.0 0.5723194122130699 3.541909287452857E-5 52-53 0.0 0.0 0.0 0.6749108147241419 7.083818574905714E-5 54-55 0.0 0.0 0.0 0.7875789491580173 7.083818574905714E-5 56-57 0.0 0.0 0.0 0.9222246307205377 7.083818574905714E-5 58-59 0.0 0.0 0.0 1.0669470442058615 7.083818574905714E-5 60-61 0.0 0.0 0.0 1.2310259919471152 7.083818574905714E-5 62-63 0.0 0.0 0.0 1.39237766953703 7.083818574905714E-5 64-65 0.0 0.0 0.0 1.5582807005613217 7.083818574905714E-5 66-67 0.0 0.0 0.0 1.7581329321058492 7.083818574905714E-5 68-69 0.0 0.0 0.0 1.9834869105200372 7.083818574905714E-5 70-71 0.0 0.0 0.0 2.2214500859975574 7.083818574905714E-5 72-73 0.0 0.0 0.0 2.469224350201322 8.854773218632144E-5 74-75 0.0 0.0 0.0 2.7261721594595896 1.0625727862358572E-4 76-77 0.0 0.0 0.0 3.0049912585678786 1.0625727862358572E-4 78-79 0.0 0.0 0.0 3.3175824627320303 1.0625727862358572E-4 80-81 0.0 0.0 0.0 3.651194898517215 1.0625727862358572E-4 82-83 0.0 0.0 0.0 3.99794781775885 1.0625727862358572E-4 84-85 0.0 1.7709546437264286E-5 0.0 4.347463426244698 1.0625727862358572E-4 86-87 0.0 3.541909287452857E-5 0.0 4.71598137805773 1.0625727862358572E-4 88-89 0.0 3.541909287452857E-5 0.0 5.109947948101111 1.0625727862358572E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4890 0.0 57.05643 1 GTATCAA 8795 0.0 42.693977 1 ATCAACG 11620 0.0 31.972448 3 TATCAAC 11695 0.0 31.781483 2 TCAACGC 11825 0.0 31.418167 4 CAACGCA 12020 0.0 30.947996 5 GTGGTAT 2130 0.0 29.455723 1 AACGCAG 12640 0.0 29.34077 6 TGGTATC 2020 0.0 28.47093 2 ACGCAGA 14410 0.0 25.63794 7 CGCAGAG 14495 0.0 25.520357 8 TACATGG 8585 0.0 23.308239 2 GCAGAGT 16320 0.0 22.200968 9 GTACATG 9405 0.0 22.034492 1 TAAGGTG 2095 0.0 21.996977 5 GTAAGGT 2090 0.0 21.822283 4 ACATGGG 8935 0.0 21.587717 3 AGGTAAG 1970 0.0 20.990398 2 GAGTACT 10955 0.0 20.763027 12-13 AGAGTAC 14990 0.0 20.622765 10-11 >>END_MODULE