FastQCFastQC Report
Thu 26 May 2016
SRR938310_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938310_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2841021
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103020.36261611582596537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77480.27271885705878274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57680.20302560241546963No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA42740.15043887391187888No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA41470.1459686500029391No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA39390.13864733840404558No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA36360.1279821585268113No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA32880.1157330410440472No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC32670.1149938701614666No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG30640.1078485516298542No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44300.055.990531
GTATCAA82850.044.8785741
ATCAACG110350.033.504783
TATCAAC111200.033.376882
TCAACGC112450.032.7945564
CAACGCA114450.032.1378635
AACGCAG120300.030.7281886
TGGTATC19300.028.316532
ACGCAGA136600.026.919057
CGCAGAG136600.026.8499648
GTGGTAT23400.024.6178461
GCAGAGT153850.023.3445829
TACATGG90800.023.3425622
TAAGGTG19600.023.2759285
GTACATG97550.022.4985331
TATATCG1503.0245634E-522.1767865
ACATGGG91700.021.9215133
AGAGTAC141350.020.85433210-11
GAGTACT101150.020.82817812-13
GTAAGGT19550.020.6619644
Produced by FastQC (version 0.11.5)