##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938310_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2841021 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.398925245536727 31.0 31.0 34.0 30.0 34.0 2 31.529011929162085 33.0 31.0 34.0 30.0 34.0 3 31.597864640916065 33.0 31.0 34.0 28.0 34.0 4 35.2384371674831 37.0 35.0 37.0 33.0 37.0 5 35.26075836820636 37.0 35.0 37.0 33.0 37.0 6 35.30231948303093 37.0 35.0 37.0 33.0 37.0 7 35.23983314449277 37.0 35.0 37.0 32.0 37.0 8 35.1981639699249 37.0 35.0 37.0 32.0 37.0 9 36.763194288250595 39.0 37.0 39.0 32.0 39.0 10-11 36.63263805512173 39.0 37.0 39.0 32.0 39.0 12-13 36.50810624067897 39.0 37.0 39.0 32.0 39.0 14-15 37.56404898098254 40.0 37.0 41.0 32.0 41.0 16-17 37.4336421659678 39.5 37.0 41.0 32.0 41.0 18-19 37.44343283629371 39.5 37.0 41.0 32.0 41.0 20-21 37.534294009090395 40.0 37.0 41.0 32.0 41.0 22-23 37.46855391072435 40.0 37.0 41.0 32.0 41.0 24-25 37.365849988437255 40.0 37.0 41.0 31.5 41.0 26-27 37.19370184169705 39.0 36.5 41.0 31.0 41.0 28-29 37.018522038379864 39.0 36.0 41.0 31.0 41.0 30-31 36.79754426313639 39.0 36.0 41.0 30.0 41.0 32-33 36.52170892084219 39.0 35.5 40.5 30.0 41.0 34-35 36.28880057556773 39.0 35.0 40.0 30.0 41.0 36-37 36.00931531305119 38.0 35.0 40.0 29.0 41.0 38-39 35.75312924473279 38.0 35.0 40.0 28.5 41.0 40-41 35.68644195167864 38.0 35.0 40.0 27.5 41.0 42-43 35.513678181189086 38.0 35.0 40.0 27.0 41.0 44-45 35.237281421010266 38.0 34.5 40.0 26.5 41.0 46-47 34.88006653241915 38.0 34.0 40.0 24.5 41.0 48-49 34.52086943391126 38.0 33.5 40.0 24.0 41.0 50-51 33.78014523651884 37.5 32.5 39.5 23.0 40.5 52-53 33.84942807532926 38.0 33.0 39.5 23.0 40.5 54-55 34.00092220367255 38.0 33.0 40.0 23.0 41.0 56-57 33.72843301756657 38.0 33.0 40.0 21.5 41.0 58-59 33.42473850070098 37.0 33.0 40.0 20.5 41.0 60-61 33.00276766697606 37.0 32.0 40.0 18.5 41.0 62-63 32.512327434397704 36.0 31.0 39.0 15.5 41.0 64-65 32.05388590932626 36.0 31.0 39.0 12.5 40.0 66-67 31.55586055154115 35.0 30.5 39.0 8.0 40.0 68-69 31.028757443186798 35.0 30.0 38.0 7.0 40.0 70-71 30.42660807505471 34.5 29.0 37.5 2.0 40.0 72-73 29.9065263861126 34.0 29.0 37.0 2.0 39.0 74-75 29.33721081259167 34.0 28.0 36.0 2.0 39.0 76-77 28.63868975273326 34.0 26.5 36.0 2.0 38.5 78-79 27.900511295059065 33.0 26.0 35.0 2.0 37.5 80-81 27.28373338317457 33.0 25.5 35.0 2.0 37.0 82-83 26.65196068596466 32.0 24.5 35.0 2.0 36.0 84-85 26.028589193814476 32.0 22.5 35.0 2.0 36.0 86-87 25.400957261491556 31.5 20.0 34.5 2.0 35.5 88-89 24.644283516383723 31.0 17.5 34.0 2.0 35.0 90-91 23.85560367206015 31.0 9.0 34.0 2.0 35.0 92-93 23.058663769116805 30.0 2.0 34.0 2.0 35.0 94-95 22.151270265161713 29.5 2.0 34.0 2.0 35.0 96-97 20.964697198647954 29.0 2.0 34.0 2.0 35.0 98-99 19.521174605889925 28.0 2.0 34.0 2.0 35.0 100-101 15.929096793019129 13.0 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.45429122971727054 1101 2 0.43144318895123845 1101 3 0.39061312894163436 1101 4 0.34671952067299117 1101 5 0.3515930065468922 1101 6 0.4000532771291532 1101 7 0.3041531497047245 1101 8 0.3588739664195373 1101 9 0.4402138989910327 1101 10-11 0.4645630106738565 1101 12-13 0.4321864782722997 1101 14-15 0.60349951053567 1101 16-17 0.5098928265218703 1101 18-19 0.4571864765614677 1101 20-21 0.4673223307941541 1101 22-23 0.5874577873530313 1101 24-25 0.5654536142484758 1101 26-27 0.6557249991046845 1101 28-29 0.5926745924058281 1101 30-31 0.508141533738403 1101 32-33 0.5425640617746694 1101 34-35 0.5527374061568935 1101 36-37 0.5079192220430215 1101 38-39 0.4897416437163358 1101 40-41 0.45549592584344367 1101 42-43 0.5939634227679065 1101 44-45 0.4252122165989505 1101 46-47 0.4790169713165753 1101 48-49 0.6118837752179687 1101 50-51 0.36861769605848593 1101 52-53 0.6050617370727664 1101 54-55 0.49789392090985274 1101 56-57 0.4306101423873372 1101 58-59 0.3378871437075972 1101 60-61 0.3809201862642695 1101 62-63 0.4294153171077255 1101 64-65 0.26030354681578416 1101 66-67 0.26180316104147394 1101 68-69 0.10579197688944575 1101 70-71 0.27234454978686173 1101 72-73 0.2835043783689031 1101 74-75 0.22540000599966348 1101 76-77 0.3206044159634587 1101 78-79 0.2412261922379102 1101 80-81 0.20401779158098776 1101 82-83 0.34268409044770465 1101 84-85 0.4775057590855525 1101 86-87 0.6333188263080878 1101 88-89 0.7674745058006103 1101 90-91 0.7286598580905022 1101 92-93 0.6915510157432259 1101 94-95 0.7603921892897105 1101 96-97 0.8387652397653689 1101 98-99 0.66430345615953 1101 100-101 0.8455104871151509 1102 1 0.4052626422518699 1102 2 0.4491913998427961 1102 3 0.521560559131327 1102 4 0.4365614668147657 1102 5 0.4330334780295857 1102 6 0.38783886476038276 1102 7 0.30372580472889865 1102 8 0.3147464517813745 1102 9 0.5255961495044801 1102 10-11 0.5465456362303627 1102 12-13 0.5090173644709779 1102 14-15 0.5212445322856212 1102 16-17 0.6051059249642918 1102 18-19 0.5787138202616333 1102 20-21 0.4998381808279575 1102 22-23 0.5889707161331188 1102 24-25 0.45819959274090394 1102 26-27 0.3596533837406781 1102 28-29 0.3550695101897574 1102 30-31 0.384127935238574 1102 32-33 0.5038325747417218 1102 34-35 0.6092879030251552 1102 36-37 0.636560265019888 1102 38-39 0.6044620835627512 1102 40-41 0.6530198762969377 1102 42-43 0.6419574526948111 1102 44-45 0.5775785596740448 1102 46-47 0.5518053016095976 1102 48-49 0.36997016268592375 1102 50-51 0.4393560461241819 1102 52-53 0.5033549084827484 1102 54-55 0.5148760999865303 1102 56-57 0.6364736981526633 1102 58-59 0.6125628080057979 1102 60-61 0.5824790386835517 1102 62-63 0.38449434403683824 1102 64-65 0.33811413731724116 1102 66-67 0.42619830844359896 1102 68-69 0.3225241272638506 1102 70-71 0.24030260619639776 1102 72-73 0.3021296032033831 1102 74-75 0.24668178579581124 1102 76-77 0.43270210574927503 1102 78-79 0.2621450477187679 1102 80-81 0.37370755188510785 1102 82-83 0.5223373669717972 1102 84-85 0.5291634748947658 1102 86-87 0.5494290940684188 1102 88-89 0.6186851039560075 1102 90-91 0.6189667810791128 1102 92-93 0.5635018774492906 1102 94-95 0.4756139621973148 1102 96-97 0.6990999720688222 1102 98-99 0.8554829282928118 1102 100-101 0.6386914645232711 1103 1 0.4123920785255244 1103 2 0.49722251917176763 1103 3 0.46492178556381347 1103 4 0.3456186667729 1103 5 0.3438088696377335 1103 6 0.31940325396131897 1103 7 0.3967376613726543 1103 8 0.27806686025994765 1103 9 0.24500760345096495 1103 10-11 0.2774727121397973 1103 12-13 0.2834997888281805 1103 14-15 0.5150969198119668 1103 16-17 0.47810452449481033 1103 18-19 0.48365496067240343 1103 20-21 0.41198800115918743 1103 22-23 0.5694781495858976 1103 24-25 0.5934748522763087 1103 26-27 0.5268741744826286 1103 28-29 0.5614458826249304 1103 30-31 0.7296844349619604 1103 32-33 0.7335474471295456 1103 34-35 0.752522912768633 1103 36-37 0.7563534839566302 1103 38-39 0.6915310404870993 1103 40-41 0.7364522226564603 1103 42-43 0.6785210444754028 1103 44-45 0.864657359755789 1103 46-47 0.7083329347164948 1103 48-49 0.6306330432672382 1103 50-51 0.7689909272836246 1103 52-53 0.8022008917269474 1103 54-55 0.8258965737538944 1103 56-57 0.827226238684041 1103 58-59 0.7837493943988179 1103 60-61 0.9660233976821857 1103 62-63 1.0709389457502922 1103 64-65 0.8287007147392842 1103 66-67 0.8695119388492394 1103 68-69 0.9589785721346935 1103 70-71 0.9626666335678493 1103 72-73 0.9015334068984302 1103 74-75 0.8271102247956499 1103 76-77 0.893985977424407 1103 78-79 0.8053906482012039 1103 80-81 0.8344459382053593 1103 82-83 1.0493345311456928 1103 84-85 1.0444346974896703 1103 86-87 0.9837952944103101 1103 88-89 0.9781474931270431 1103 90-91 1.1631363923865337 1103 92-93 1.1422149905284797 1103 94-95 1.1439025486202326 1103 96-97 1.4392666578403528 1103 98-99 1.64261505586013 1103 100-101 1.3333533582969128 1104 1 0.3987820115116385 1104 2 0.32593267214549826 1104 3 0.3474225550469612 1104 4 0.3553506353789544 1104 5 0.3570001300487107 1104 6 0.3413947681446956 1104 7 0.2887414872863374 1104 8 0.2846519206420268 1104 9 0.3419903839402565 1104 10-11 0.4380562944377715 1104 12-13 0.36341737802902685 1104 14-15 0.5178368596352456 1104 16-17 0.5146262239194712 1104 18-19 0.5996835361597874 1104 20-21 0.5768773067205828 1104 22-23 0.6332780264685951 1104 24-25 0.5928071414318907 1104 26-27 0.5753491966007331 1104 28-29 0.610506412560845 1104 30-31 0.49791363560306223 1104 32-33 0.5042010028664592 1104 34-35 0.4704041185837582 1104 36-37 0.5261221944768266 1104 38-39 0.407565486756404 1104 40-41 0.42161402672116566 1104 42-43 0.37424572606400375 1104 44-45 0.5296647453443981 1104 46-47 0.5691457553445431 1104 48-49 0.4881529713102921 1104 50-51 0.39871213520546434 1104 52-53 0.39672144695312994 1104 54-55 0.5017900922657006 1104 56-57 0.5581765781505297 1104 58-59 0.5919324067235934 1104 60-61 0.536066476658462 1104 62-63 0.42876539867218355 1104 64-65 0.5206831956495748 1104 66-67 0.6201079560914629 1104 68-69 0.5411291120291928 1104 70-71 0.5482887866614128 1104 72-73 0.4092963080020908 1104 74-75 0.22184221272938132 1104 76-77 0.4479695687097518 1104 78-79 0.532720732552022 1104 80-81 0.5626256551611881 1104 82-83 0.4910305396421393 1104 84-85 0.5062685147397197 1104 86-87 0.748085393662766 1104 88-89 0.7486237174266641 1104 90-91 0.7080147753944139 1104 92-93 0.5936779990457026 1104 94-95 0.516250730924682 1104 96-97 0.3523506375636849 1104 98-99 0.28421408773678536 1104 100-101 0.09329581738626125 1105 1 0.46946093865984295 1105 2 0.4815499446656304 1105 3 0.4558148766886383 1105 4 0.36966317348358047 1105 5 0.3697160887524191 1105 6 0.33754810860504136 1105 7 0.35540774092584826 1105 8 0.34903769075524593 1105 9 0.4641503920355845 1105 10-11 0.4906644805900271 1105 12-13 0.43207029226830684 1105 14-15 0.4894962558767446 1105 16-17 0.45052613091952054 1105 18-19 0.46961801628595623 1105 20-21 0.49340119219549905 1105 22-23 0.5123451679329207 1105 24-25 0.5485065375173619 1105 26-27 0.7006420730067262 1105 28-29 0.5720380962178382 1105 30-31 0.592250528542067 1105 32-33 0.7021767912148249 1105 34-35 0.5965875274207448 1105 36-37 0.7747293177725467 1105 38-39 0.6991093065278662 1105 40-41 0.7491241934992345 1105 42-43 0.7439510908507287 1105 44-45 0.7654695184238349 1105 46-47 0.6878473494228317 1105 48-49 0.6098355888561073 1105 50-51 0.56191384825852 1105 52-53 0.49438217589557354 1105 54-55 0.6280394133523188 1105 56-57 0.725833821602734 1105 58-59 0.754597012384771 1105 60-61 0.7413962708080177 1105 62-63 0.7611450982041035 1105 64-65 0.7429674101878163 1105 66-67 0.6864708642339927 1105 68-69 0.6854192870081448 1105 70-71 0.6626850316021198 1105 72-73 0.6986931896929853 1105 74-75 0.6889026090013957 1105 76-77 0.6243389315488237 1105 78-79 0.795720934246944 1105 80-81 0.756998125800127 1105 82-83 0.6931889736451033 1105 84-85 0.5723780995625312 1105 86-87 0.6743206374072948 1105 88-89 0.6243176317239936 1105 90-91 0.6612944633845217 1105 92-93 0.851844176848843 1105 94-95 1.0465875752182647 1105 96-97 0.9207780504510943 1105 98-99 0.9976378373028219 1105 100-101 0.8917354696449333 1106 1 0.2775051403714137 1106 2 0.3627149799454905 1106 3 0.1924631550105218 1106 4 0.38453843210549365 1106 5 0.38205406753819915 1106 6 0.0741454992113475 1106 7 0.1992260066816698 1106 8 0.2287240023723598 1106 9 0.20019581138474507 1106 10-11 0.024052208765269256 1106 12-13 0.23213841762445497 1106 14-15 0.3610240288790223 1106 16-17 0.16321741257019795 1106 18-19 -0.18560269657300665 1106 20-21 0.24686270297383572 1106 22-23 0.16997433677147455 1106 24-25 0.3411529646266658 1106 26-27 0.09921024856291893 1106 28-29 -0.0023870215918009308 1106 30-31 0.1625809044280757 1106 32-33 0.3570362471380406 1106 34-35 0.2968154447882654 1106 36-37 0.20714481551171104 1106 38-39 0.30457249247479723 1106 40-41 0.4033906470793269 1106 42-43 -0.05836881591930876 1106 44-45 0.18134903006680503 1106 46-47 -0.010712505304205422 1106 48-49 0.32080711786691296 1106 50-51 -0.059178284638186085 1106 52-53 0.2382113527111258 1106 54-55 0.10345548919426761 1106 56-57 -0.3433559359379288 1106 58-59 -0.7263241056962713 1106 60-61 0.0203255932724673 1106 62-63 0.23257978336273055 1106 64-65 0.27918903398272477 1106 66-67 -0.20739478211105578 1106 68-69 -0.3860315355161319 1106 70-71 -0.18746130496373326 1106 72-73 0.29554993343849745 1106 74-75 0.1978422282054133 1106 76-77 0.32675284305829777 1106 78-79 0.39471954652518804 1106 80-81 0.39459358038100234 1106 82-83 0.3511010048305643 1106 84-85 0.3341170015706716 1106 86-87 0.49435004225483326 1106 88-89 0.29596384473813586 1106 90-91 0.30550463331740474 1106 92-93 0.22322100588399962 1106 94-95 0.251196420603506 1106 96-97 0.40645625304197 1106 98-99 0.26697150163552763 1106 100-101 -0.2046450613569153 1107 1 0.3412747705511592 1107 2 0.2854300093406792 1107 3 0.20800681491179418 1107 4 0.2528715796624894 1107 5 0.36712190276040957 1107 6 0.23278036096913723 1107 7 0.21370022159758406 1107 8 0.26507796941339734 1107 9 0.25467841533332347 1107 10-11 0.3539521491825468 1107 12-13 0.26733061107896106 1107 14-15 0.30573900545105914 1107 16-17 0.30174198585537937 1107 18-19 0.24212023615044842 1107 20-21 0.3227818817618484 1107 22-23 0.4684099209530075 1107 24-25 0.29824019758437004 1107 26-27 0.3310563192143334 1107 28-29 0.35793703948787936 1107 30-31 0.4779581714029959 1107 32-33 0.4195985549546606 1107 34-35 0.5122148258620882 1107 36-37 0.5235372494458446 1107 38-39 0.4376037937731212 1107 40-41 0.38923214002273454 1107 42-43 0.45078189972247174 1107 44-45 0.4606420414296011 1107 46-47 0.32278959352074565 1107 48-49 0.29577700195591206 1107 50-51 0.37905253820773765 1107 52-53 0.32825154754797836 1107 54-55 0.3311136464093991 1107 56-57 0.3292532233279033 1107 58-59 0.43039670022385934 1107 60-61 0.3059285091104229 1107 62-63 0.5000148925835433 1107 64-65 0.3790017356227935 1107 66-67 0.19043091850075555 1107 68-69 0.29110575409216466 1107 70-71 0.27356830330783666 1107 72-73 0.26666495853621086 1107 74-75 0.3914208355454143 1107 76-77 0.36620121715722576 1107 78-79 0.48925161174476983 1107 80-81 0.33589016044008346 1107 82-83 0.3975598616390883 1107 84-85 0.2612593272844883 1107 86-87 0.34562813868752684 1107 88-89 0.5119299555530468 1107 90-91 0.7262495382334926 1107 92-93 0.49785595538193306 1107 94-95 0.3898142177975217 1107 96-97 0.1923254109916961 1107 98-99 0.10749572216949943 1107 100-101 -0.2809216917233339 1108 1 0.22626269760804263 1108 2 0.24730952068531664 1108 3 0.2704264495814641 1108 4 0.2705570752252129 1108 5 0.1975248009498074 1108 6 0.05812205770431689 1108 7 0.18834832177398653 1108 8 0.0872955573179155 1108 9 0.057183764483859534 1108 10-11 0.20123176674176335 1108 12-13 0.1657908839240605 1108 14-15 0.2208363958511086 1108 16-17 0.054073647703681615 1108 18-19 0.1526726629486248 1108 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>>Per sequence quality scores pass #Quality Count 2 7576.0 3 2280.0 4 2287.0 5 2509.0 6 2975.0 7 3688.0 8 4703.0 9 6288.0 10 8019.0 11 10414.0 12 13593.0 13 16714.0 14 19841.0 15 24048.0 16 28104.0 17 33131.0 18 30709.0 19 27086.0 20 28392.0 21 30543.0 22 33711.0 23 37584.0 24 42729.0 25 48487.0 26 55775.0 27 64292.0 28 75088.0 29 88268.0 30 104467.0 31 125361.0 32 151458.0 33 186049.0 34 234211.0 35 297219.0 36 367374.0 37 394883.0 38 215995.0 39 15170.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.90101745236697 18.9397409383975 14.853852514364396 23.305389094871128 2 15.267221002989078 19.919694950684445 40.689769804399944 24.123314241926533 3 19.10028675291674 23.767002954861503 29.846601451477557 27.2861088407442 4 12.60585762859895 16.537191697965696 37.3579569966315 33.498993676803856 5 13.526586552058237 37.43146756527641 34.4988587172623 14.543087165403055 6 32.25856669840073 36.36011427078061 17.347469605219178 14.033849425599488 7 28.50038732394366 32.73672535211268 20.525211267605634 18.237676056338028 8 26.364160996930014 35.06536261843421 19.728817402614503 18.841658982021276 9 26.2645586932424 16.77786839180755 20.104835267919395 36.85273764703065 10-11 24.77604277442155 26.667865096055117 27.620352359368244 20.93573977015509 12-13 25.980899193906897 23.884458521461337 27.720025035806305 22.414617248825454 14-15 22.335353714607237 25.4913103887315 26.238689491552634 25.93464640510863 16-17 21.860755966000667 28.608617715940674 27.195241600294633 22.335384717764022 18-19 21.913117210617973 26.950577374036644 29.593872424696293 21.54243299064909 20-21 22.311696370213436 26.26179032657937 29.92256699497397 21.50394630823323 22-23 21.91102448980814 26.32495466496977 29.790172629667694 21.9738482155544 24-25 21.77399826318597 26.40968206018337 29.95940800389136 21.856911672739294 26-27 21.416968696167565 26.808386779480692 29.6017436623989 22.17290086195284 28-29 21.53175219000201 27.296123767312295 29.50643544457045 21.66568859811524 30-31 21.711588276964367 26.65564485271369 29.38929301619763 22.243473854124314 32-33 21.233106701255746 26.7324957930177 29.50307338170945 22.5313241240171 34-35 22.213639394580213 26.961672558532314 29.18942989266809 21.635258154219386 36-37 22.26526459762764 26.421114424440823 29.21117241631886 22.102448561612672 38-39 21.68624708238984 26.81101314986416 29.185748454694238 22.31699131305176 40-41 22.227558048528714 26.647364980025273 29.290253733797268 21.834823237648745 42-43 21.92435652348412 26.66684269635231 29.046852061131588 22.361948719031975 44-45 21.94341581893062 26.98345594705903 29.183850188320605 21.889278045689746 46-47 22.349286309588244 27.242635378521257 28.350358231311557 22.057720080578942 48-49 22.012879828747703 27.325879591836017 28.27215344273514 22.389087136681145 50-51 22.24438954536238 27.543519921373377 28.271228062270488 21.94086247099376 52-53 22.569597474482215 27.134742625575374 28.33192056784724 21.96373933209517 54-55 21.929191845160272 27.437323944157782 28.53308564947313 22.10039856120882 56-57 22.121745105524734 27.61615253841419 28.12974396640077 22.132358389660304 58-59 22.002012513348255 27.16248818744078 28.250202897040598 22.58529640217036 60-61 21.668419740882957 27.349313062444647 28.589929891112263 22.39233730556013 62-63 21.86585514165989 27.322581718194527 28.748218218375015 22.063344921770568 64-65 21.569203783489712 27.452389128266468 28.51437254042984 22.46403454781398 66-67 21.505059921957926 27.511089341823826 28.493012874249334 22.490837861968913 68-69 22.194334811327 26.999726836356675 28.595322847041032 22.210615505275296 70-71 21.913426707801847 27.154148847817076 28.176296854904837 22.75612758947624 72-73 21.823726665434478 27.289731470968764 28.279565917672222 22.60697594592454 74-75 21.913381943053327 27.63149602861803 28.033298984279586 22.421823044049052 76-77 22.038432597742624 27.24331697872434 28.183705529031265 22.53454489450177 78-79 22.03157299109166 27.505075456439076 28.12285992620238 22.340491626266886 80-81 22.314417597410895 27.447394719917828 27.822443957340592 22.415743725330685 82-83 22.1900331309058 27.565022746697114 27.953585755259336 22.291358367137747 84-85 22.120870624441967 27.70304840347913 27.88638944862341 22.28969152345549 86-87 22.358973438324757 27.649782887130346 27.569031085747397 22.4222125887975 88-89 22.21825340728826 27.675565633880872 27.45922512106108 22.646955837769788 90-91 22.129794796208177 27.69738866748873 27.526808041954844 22.64600849434825 92-93 22.344065454195952 27.2804150544369 27.752285462761648 22.623234028605495 94-95 22.06845594906999 27.10036818698279 27.94328645876795 22.887889405179266 96-97 22.400958439736165 26.74942980406034 27.935666229171673 22.91394552703182 98-99 22.10893143254065 26.975907512562365 27.54108622324304 23.374074831653953 100-101 22.645784278086794 26.48335752946751 27.865823434767968 23.005034757677727 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 673.0 1 472.0 2 353.0 3 475.0 4 437.5 5 434.0 6 811.0 7 1828.5 8 2888.5 9 3169.5 10 2665.5 11 2123.0 12 2106.5 13 2290.5 14 2491.0 15 2723.0 16 2948.0 17 3224.0 18 3676.5 19 4240.0 20 4844.0 21 5792.0 22 7126.5 23 8822.0 24 11272.5 25 14416.5 26 18613.0 27 23696.5 28 28232.0 29 32801.0 30 37437.5 31 44220.0 32 54698.0 33 59215.0 34 59882.0 35 64083.5 36 69583.5 37 74762.0 38 78504.5 39 81469.0 40 85384.0 41 90070.0 42 92462.5 43 94523.0 44 96786.0 45 100999.0 46 103015.0 47 103796.5 48 104303.5 49 101123.5 50 98377.0 51 95454.5 52 93106.0 53 92633.5 54 93203.5 55 88562.0 56 80738.0 57 73208.0 58 67175.0 59 59019.5 60 46229.5 61 37502.5 62 31865.0 63 23802.5 64 16241.5 65 12114.5 66 9118.5 67 7002.0 68 5523.0 69 4421.5 70 3608.5 71 2668.5 72 2006.5 73 1612.0 74 1188.5 75 874.0 76 700.5 77 488.0 78 323.5 79 227.5 80 148.5 81 89.0 82 52.0 83 25.0 84 17.0 85 16.0 86 11.0 87 6.0 88 6.0 89 6.0 90 5.0 91 3.5 92 3.0 93 2.0 94 2.5 95 1.0 96 0.5 97 0.5 98 0.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17655624509639317 2 0.047482929552439074 3 0.045547005812347045 4 0.051953153461378854 5 0.05885208169879772 6 0.06335750422119373 7 0.03593778433879932 8 0.03315709387575805 9 0.0400208234997207 10-11 0.041991945853268944 12-13 0.03831369074709409 14-15 0.03389626475833864 16-17 0.031907543098062284 18-19 0.04003842280644881 20-21 0.049454051905987326 22-23 0.04679655659004281 24-25 0.06738774546193076 26-27 0.05211154722193184 28-29 0.043663879992439335 30-31 0.05281551949105621 32-33 0.04915486369160946 34-35 0.04827489835520399 36-37 0.046215779468015195 38-39 0.025536594062486692 40-41 0.024005454377141175 42-43 0.02080238055262527 44-45 0.004259032228202467 46-47 0.011562744520367855 48-49 0.01055958403686562 50-51 0.008905249204423338 52-53 0.009046043658248215 54-55 0.010665179877234277 56-57 0.008500465149676823 58-59 0.010365991662856417 60-61 0.010453988196496964 62-63 0.004030241240737045 64-65 0.007198116451796731 66-67 0.004135837081105701 68-69 0.008201276935298964 70-71 0.022175126477417803 72-73 0.01921844294709543 74-75 0.02020400412386955 76-77 0.011738737587648948 78-79 0.005051001030967388 80-81 0.006828531010506434 82-83 0.005948565674100965 84-85 0.004945405190598732 86-87 0.007655698426727575 88-89 0.028950859567739906 90-91 0.03572659265806201 92-93 0.021911136876496158 94-95 0.031256368749122236 96-97 0.034793829401472215 98-99 0.02083757916608149 100-101 0.12131202127685786 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2841021.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.16663295957921 #Duplication Level Percentage of deduplicated Percentage of total 1 75.22604349617754 41.49967530554967 2 14.047601938548146 15.499178002123179 3 4.782929180233503 7.915742956728081 4 2.1710567342922156 4.790795599804854 5 1.1520206674134827 3.177655066052454 6 0.6411932897779185 2.122348492399412 7 0.42318635994361126 1.63420366147567 8 0.28419437581266177 1.2542437455707063 9 0.19353751463471344 0.9609131730386182 >10 0.9713388572190893 9.577772608266507 >50 0.06288764013017875 2.3958679748715244 >100 0.037486226377614104 3.8774210334203993 >500 0.003389775787286256 1.2716171133542198 >1k 0.002942069551042873 3.182183014170726 >5k 1.2791606743664665E-4 0.4768917009782959 >10k+ 6.395803371832333E-5 0.36349055219579796 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10302 0.36261611582596537 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7748 0.27271885705878274 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5768 0.20302560241546963 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4274 0.15043887391187888 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4147 0.1459686500029391 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3939 0.13864733840404558 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3636 0.1279821585268113 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3288 0.1157330410440472 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3267 0.1149938701614666 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3064 0.1078485516298542 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.519861345621873E-5 0.0 12-13 0.0 0.0 0.0 3.519861345621873E-5 0.0 14-15 0.0 0.0 0.0 3.519861345621873E-5 0.0 16-17 0.0 0.0 0.0 3.519861345621873E-5 0.0 18-19 0.0 0.0 0.0 7.039722691243746E-5 3.519861345621873E-5 20-21 0.0 0.0 0.0 1.055958403686562E-4 3.519861345621873E-5 22-23 0.0 0.0 0.0 1.4079445382487492E-4 3.519861345621873E-5 24-25 0.0 0.0 0.0 3.8718474801840606E-4 3.519861345621873E-5 26-27 0.0 0.0 0.0 7.743694960368121E-4 3.519861345621873E-5 28-29 0.0 0.0 0.0 0.002199913341013671 3.519861345621873E-5 30-31 0.0 0.0 0.0 0.007849290800736777 3.519861345621873E-5 32-33 0.0 0.0 0.0 0.02004561036331657 3.519861345621873E-5 34-35 0.0 0.0 0.0 0.040390408941010994 3.519861345621873E-5 36-37 0.0 0.0 0.0 0.07411068063206855 3.519861345621873E-5 38-39 0.0 0.0 0.0 0.1245854923282862 3.519861345621873E-5 40-41 0.0 0.0 0.0 0.18127285929952647 3.519861345621873E-5 42-43 0.0 0.0 0.0 0.24308162452864657 3.519861345621873E-5 44-45 0.0 0.0 0.0 0.3199377970103002 3.519861345621873E-5 46-47 0.0 0.0 0.0 0.4150444505690032 3.519861345621873E-5 48-49 0.0 0.0 0.0 0.5124038153889042 3.519861345621873E-5 50-51 0.0 0.0 0.0 0.6196539905900027 3.519861345621873E-5 52-53 0.0 0.0 0.0 0.7263585872825298 7.039722691243746E-5 54-55 0.0 0.0 0.0 0.8448019215627058 7.039722691243746E-5 56-57 0.0 0.0 0.0 0.9881130762497004 7.039722691243746E-5 58-59 0.0 0.0 0.0 1.138499152241395 7.039722691243746E-5 60-61 0.0 0.0 0.0 1.3046014091412912 7.039722691243746E-5 62-63 0.0 0.0 0.0 1.4669198150946436 7.039722691243746E-5 64-65 0.0 0.0 0.0 1.6402905856732493 7.039722691243746E-5 66-67 0.0 0.0 0.0 1.8448649270807924 7.039722691243746E-5 68-69 0.0 0.0 0.0 2.0743070888951545 7.039722691243746E-5 70-71 0.0 0.0 0.0 2.314185639599285 7.039722691243746E-5 72-73 0.0 0.0 0.0 2.5695339809174236 7.039722691243746E-5 74-75 0.0 0.0 0.0 2.82611427370653 7.039722691243746E-5 76-77 0.0 0.0 0.0 3.108987930747432 7.039722691243746E-5 78-79 0.0 0.0 0.0 3.4239627232604053 7.039722691243746E-5 80-81 0.0 0.0 0.0 3.759247115737617 7.039722691243746E-5 82-83 0.0 0.0 0.0 4.109191730719344 7.039722691243746E-5 84-85 0.0 0.0 0.0 4.467073633035447 7.039722691243746E-5 86-87 0.0 0.0 0.0 4.840390127352103 7.039722691243746E-5 88-89 0.0 0.0 0.0 5.242763077076868 8.799653364054683E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4430 0.0 55.99053 1 GTATCAA 8285 0.0 44.878574 1 ATCAACG 11035 0.0 33.50478 3 TATCAAC 11120 0.0 33.37688 2 TCAACGC 11245 0.0 32.794556 4 CAACGCA 11445 0.0 32.137863 5 AACGCAG 12030 0.0 30.728188 6 TGGTATC 1930 0.0 28.31653 2 ACGCAGA 13660 0.0 26.91905 7 CGCAGAG 13660 0.0 26.849964 8 GTGGTAT 2340 0.0 24.617846 1 GCAGAGT 15385 0.0 23.344582 9 TACATGG 9080 0.0 23.342562 2 TAAGGTG 1960 0.0 23.275928 5 GTACATG 9755 0.0 22.498533 1 TATATCG 150 3.0245634E-5 22.176786 5 ACATGGG 9170 0.0 21.921513 3 AGAGTAC 14135 0.0 20.854332 10-11 GAGTACT 10115 0.0 20.828178 12-13 GTAAGGT 1955 0.0 20.661964 4 >>END_MODULE