FastQCFastQC Report
Thu 26 May 2016
SRR938310_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938310_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2841021
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97570.34343287149232615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81180.2857423440375837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61150.21523952128477755No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40630.14301196647261671No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC39520.13910492037897643No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA38470.13540906596607347No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA36980.13016447256109687No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA34250.12055525108754916No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG32420.11411390482506115No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49750.056.9684331
GTATCAA92000.042.487881
TGGTATC19150.034.2682082
ATCAACG120850.031.741833
TCAACGC122650.031.275444
TATCAAC123950.031.1523322
CAACGCA125700.030.5543865
AACGCAG130250.029.46426
GTGGTAT24400.028.2591631
CGCAGAG148100.025.8167658
ACGCAGA148650.025.7531977
TAAGGTG20900.024.107775
TACATGG92950.023.4825022
GTAAGGT21200.023.3181954
GTACATG98100.023.2192171
GCAGAGT167650.022.4945839
ACATGGG92950.022.1945043
GGTAAGG21600.022.006523
AGGTAAG21400.021.3323442
AGAGTAC153850.021.00833710-11
Produced by FastQC (version 0.11.5)