##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938310_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2841021 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.651269033210244 31.0 31.0 34.0 30.0 34.0 2 31.899030313397894 31.0 31.0 34.0 30.0 34.0 3 31.957354063908713 31.0 31.0 34.0 30.0 34.0 4 35.655588959039726 37.0 35.0 37.0 33.0 37.0 5 35.521757142942626 37.0 35.0 37.0 33.0 37.0 6 35.55429051738794 37.0 35.0 37.0 33.0 37.0 7 35.48814457900875 37.0 35.0 37.0 33.0 37.0 8 35.47265754107414 37.0 35.0 37.0 33.0 37.0 9 37.03050030253208 39.0 37.0 39.0 33.0 39.0 10-11 36.932691979397546 39.0 37.0 39.0 33.0 39.0 12-13 36.81660096845465 39.0 37.0 39.0 32.0 39.0 14-15 37.81415396084718 40.0 37.0 41.0 32.5 41.0 16-17 37.727496382462505 39.0 37.0 41.0 32.0 41.0 18-19 37.856647486942194 39.5 37.0 41.0 32.5 41.0 20-21 37.91342883421136 40.0 37.0 41.0 32.5 41.0 22-23 37.810143078843836 40.0 37.0 41.0 32.0 41.0 24-25 37.69430602589703 40.0 37.0 41.0 32.0 41.0 26-27 37.482212908669105 39.0 36.0 41.0 32.0 41.0 28-29 37.3475113348335 39.0 36.0 41.0 31.5 41.0 30-31 37.08156310706609 39.0 36.0 40.5 31.0 41.0 32-33 36.8283214027633 39.0 35.5 40.0 30.5 41.0 34-35 36.64561138407636 39.0 35.0 40.0 30.0 41.0 36-37 36.61194778215297 39.0 35.0 40.0 30.0 41.0 38-39 36.458133361210635 39.0 35.0 40.0 30.0 41.0 40-41 36.25752924740789 39.0 35.0 40.0 30.0 41.0 42-43 36.01880661916966 38.0 35.0 40.0 29.5 41.0 44-45 35.72807011986184 38.0 35.0 40.0 28.5 41.0 46-47 35.3195844029312 38.0 34.0 40.0 27.0 41.0 48-49 34.922763506499955 38.0 34.0 40.0 26.0 41.0 50-51 34.518223554137755 38.0 33.0 40.0 24.0 41.0 52-53 33.987348562365426 37.5 33.0 40.0 23.5 41.0 54-55 33.644194991870876 37.0 32.5 40.0 23.0 41.0 56-57 33.278499877332834 37.0 32.0 39.5 22.0 41.0 58-59 32.79945150000651 36.0 31.0 39.0 20.0 40.0 60-61 32.407821871080856 36.0 31.0 39.0 18.5 40.0 62-63 31.917062035092314 35.0 30.5 39.0 17.0 40.0 64-65 31.384905109817915 35.0 29.5 38.0 13.5 40.0 66-67 30.86176395739419 34.5 29.0 38.0 9.5 40.0 68-69 30.285487330083093 34.0 28.5 37.5 8.0 39.5 70-71 29.66425468167958 34.0 28.0 36.5 7.0 39.0 72-73 29.180622036936718 34.0 27.0 36.0 4.0 39.0 74-75 28.624026186360467 33.0 26.0 36.0 2.0 38.5 76-77 27.439576828189583 31.5 25.5 34.5 2.0 36.5 78-79 28.121345107973504 33.0 26.0 35.0 2.0 37.0 80-81 27.816527403352527 33.0 26.0 35.0 2.0 37.0 82-83 27.279937916685583 32.0 25.5 35.0 2.0 36.0 84-85 26.703372836737216 32.0 25.0 35.0 2.0 36.0 86-87 26.053109427913416 31.5 23.5 34.0 2.0 35.0 88-89 25.315095699750195 31.0 20.5 34.0 2.0 35.0 90-91 24.681338856699757 31.0 19.0 34.0 2.0 35.0 92-93 23.85538227278151 30.5 12.0 34.0 2.0 35.0 94-95 22.97169644293372 30.0 3.5 34.0 2.0 35.0 96-97 21.844147755331623 29.0 2.0 34.0 2.0 35.0 98-99 20.474222119442274 29.0 2.0 34.0 2.0 35.0 100-101 16.929493657385848 21.5 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2753688617152683 1101 2 0.25078409616536135 1101 3 0.24757190494703707 1101 4 0.19886251902600804 1101 5 0.10950221837084229 1101 6 0.15363578084146923 1101 7 0.2807397492219721 1101 8 0.23060183584022553 1101 9 0.22492363334600896 1101 10-11 0.29307894546830227 1101 12-13 0.3502992641676599 1101 14-15 0.42305088388240364 1101 16-17 0.3587939999792198 1101 18-19 0.41357639467688045 1101 20-21 0.44395083224377885 1101 22-23 0.3647247931923161 1101 24-25 0.40683081539951615 1101 26-27 0.5218518168572359 1101 28-29 0.4041334487146955 1101 30-31 0.4088693041978573 1101 32-33 0.30357902236525547 1101 34-35 0.28536701333450054 1101 36-37 0.42723866300159585 1101 38-39 0.4345451428648701 1101 40-41 0.3166794324324087 1101 42-43 0.42185653722318506 1101 44-45 0.3784678984203893 1101 46-47 0.452295702376567 1101 48-49 0.4801154041690907 1101 50-51 0.6527790402388547 1101 52-53 0.6761246352559738 1101 54-55 0.4102152304049298 1101 56-57 0.5327058250678149 1101 58-59 0.58614341416218 1101 60-61 0.5377094183014179 1101 62-63 0.6550548434042476 1101 64-65 0.6836718382018567 1101 66-67 0.4473141009483754 1101 68-69 0.45438551312097175 1101 70-71 0.41596878149275085 1101 72-73 0.3576429369508958 1101 74-75 0.42992418549632916 1101 76-77 0.5322175645060945 1101 78-79 0.4829323141825199 1101 80-81 0.5706801648401871 1101 82-83 0.5070137957737337 1101 84-85 0.4819749208106394 1101 86-87 0.39325125228173263 1101 88-89 0.5443734060310597 1101 90-91 0.47437417506413837 1101 92-93 0.37228238316718176 1101 94-95 0.45310923988670027 1101 96-97 0.46299375885079996 1101 98-99 0.3729498565007745 1101 100-101 0.24724341364388636 1102 1 0.3006434468079462 1102 2 0.350287865730742 1102 3 0.3840595119427377 1102 4 0.18464787703904761 1102 5 0.1419829878469301 1102 6 0.18208164835176888 1102 7 0.23864180430482662 1102 8 0.3153921816707381 1102 9 0.25460178517466403 1102 10-11 0.3523784824049372 1102 12-13 0.36315150485855696 1102 14-15 0.47799596686387247 1102 16-17 0.4564716670583451 1102 18-19 0.4410470226826462 1102 20-21 0.4274961371895003 1102 22-23 0.5696647735471956 1102 24-25 0.6073710559883523 1102 26-27 0.5851365051505439 1102 28-29 0.4600352741793543 1102 30-31 0.42826374998882955 1102 32-33 0.4062304747003438 1102 34-35 0.47850780755833 1102 36-37 0.5000767439037404 1102 38-39 0.5193941689010231 1102 40-41 0.4645178322487098 1102 42-43 0.4841685373252389 1102 44-45 0.5886731515744543 1102 46-47 0.4762939929966592 1102 48-49 0.7077843967830404 1102 50-51 0.7522611236412828 1102 52-53 0.5569623589742534 1102 54-55 0.6607943785214232 1102 56-57 0.5733412296423168 1102 58-59 0.692643522592995 1102 60-61 0.7496336187836192 1102 62-63 0.7619689024161325 1102 64-65 0.7199183460917915 1102 66-67 0.8032235803528991 1102 68-69 0.763477755852918 1102 70-71 0.7442456244976867 1102 72-73 0.5642648705912379 1102 74-75 0.6236466791499353 1102 76-77 0.6287407623221704 1102 78-79 0.7267504820844444 1102 80-81 0.5847225931801745 1102 82-83 0.6183830856156796 1102 84-85 0.5216369993655761 1102 86-87 0.41215839723459524 1102 88-89 0.6377399914809239 1102 90-91 0.44491734966326035 1102 92-93 0.6260002844568682 1102 94-95 0.6113748571131694 1102 96-97 0.49761444234763275 1102 98-99 0.42877195342516217 1102 100-101 0.062094608296334286 1103 1 0.33883140875105866 1103 2 0.24858213377362404 1103 3 0.260296136462415 1103 4 0.28019179247262116 1103 5 0.1980468711843315 1103 6 0.187927736257393 1103 7 0.3026893604606684 1103 8 0.3130250892148325 1103 9 0.28503931975031094 1103 10-11 0.37490174465156656 1103 12-13 0.37618396983435787 1103 14-15 0.494857669256632 1103 16-17 0.46652339970324164 1103 18-19 0.5009057546846165 1103 20-21 0.5357620220519763 1103 22-23 0.5819420565367253 1103 24-25 0.5679844715888862 1103 26-27 0.45191360622855115 1103 28-29 0.4842894369627544 1103 30-31 0.5751333767462725 1103 32-33 0.6471276722662154 1103 34-35 0.5601016016344929 1103 36-37 0.5750154422147062 1103 38-39 0.45356313230441003 1103 40-41 0.5132665052914192 1103 42-43 0.5573562749938787 1103 44-45 0.619923805708332 1103 46-47 0.5414553211002442 1103 48-49 0.43760256002495623 1103 50-51 0.5168790845377842 1103 52-53 0.717872813653365 1103 54-55 0.6201598312817396 1103 56-57 0.7348384112597799 1103 58-59 0.7586345416463587 1103 60-61 0.7230655664329717 1103 62-63 0.685634344710305 1103 64-65 0.5629861467566393 1103 66-67 0.610588504822239 1103 68-69 0.44361476263597766 1103 70-71 0.20675714971361359 1103 72-73 0.4206591849841743 1103 74-75 0.422892493939095 1103 76-77 0.38060498157289757 1103 78-79 0.6428603666080122 1103 80-81 0.4441336484164857 1103 82-83 0.5409145712596235 1103 84-85 0.6405496918055356 1103 86-87 0.8110608228876082 1103 88-89 0.8533121156592038 1103 90-91 1.0049986117803122 1103 92-93 0.9975728721357306 1103 94-95 0.8554297146490413 1103 96-97 0.8072194950022933 1103 98-99 0.7830077506212376 1103 100-101 0.5247228808346733 1104 1 0.257637528473456 1104 2 0.31797465521889023 1104 3 0.195103760844475 1104 4 0.18919660554740858 1104 5 0.11547553152021806 1104 6 0.10411978765355201 1104 7 0.12843621814276673 1104 8 0.17153791626932957 1104 9 0.2196900275540088 1104 10-11 0.3054138531996671 1104 12-13 0.28643837763192437 1104 14-15 0.46020797615598497 1104 16-17 0.44063276826319964 1104 18-19 0.4512150820766223 1104 20-21 0.3651313092134174 1104 22-23 0.4064716329561904 1104 24-25 0.4132217212411078 1104 26-27 0.44836063558874173 1104 28-29 0.4388149763136582 1104 30-31 0.4953518664719141 1104 32-33 0.37693484003190036 1104 34-35 0.41721969519107915 1104 36-37 0.5204505710409251 1104 38-39 0.4991807989609356 1104 40-41 0.507161435690044 1104 42-43 0.5264420178845555 1104 44-45 0.3753175563938598 1104 46-47 0.2413664983442274 1104 48-49 0.33576907617733553 1104 50-51 0.4241026105728878 1104 52-53 0.4348853007823479 1104 54-55 0.40940564599406315 1104 56-57 0.48544209917384507 1104 58-59 0.4436654006987908 1104 60-61 0.25012564313485797 1104 62-63 0.325309789524713 1104 64-65 0.3856249327659178 1104 66-67 0.3321510895318269 1104 68-69 0.3102575354618402 1104 70-71 0.2821443918038824 1104 72-73 0.2325502725573969 1104 74-75 0.1495596993647581 1104 76-77 0.24327978229246483 1104 78-79 0.2507769348243265 1104 80-81 0.3638300492647737 1104 82-83 0.2663297056038907 1104 84-85 0.3891452340700887 1104 86-87 0.4932688143022581 1104 88-89 0.15945213211672282 1104 90-91 0.08913060097034275 1104 92-93 0.14232479173645984 1104 94-95 0.1124552648717696 1104 96-97 -0.026391854886156807 1104 98-99 -0.07941965583160027 1104 100-101 -0.30285762981221964 1105 1 0.2583887789705237 1105 2 0.244000574508334 1105 3 0.16509894448896745 1105 4 0.2392447117376051 1105 5 0.15602401322093584 1105 6 0.26415369718270654 1105 7 0.24520931961183123 1105 8 0.22478939221038274 1105 9 0.2778241728069375 1105 10-11 0.31445995093060475 1105 12-13 0.3537616312670764 1105 14-15 0.4950135447716093 1105 16-17 0.2795374936838968 1105 18-19 0.41031810871664476 1105 20-21 0.3479895351195381 1105 22-23 0.41336127003611267 1105 24-25 0.41975913794443187 1105 26-27 0.34524244121494974 1105 28-29 0.3002579623898285 1105 30-31 0.43214144542974253 1105 32-33 0.3627930198166567 1105 34-35 0.48919099576998093 1105 36-37 0.47434997185325045 1105 38-39 0.3720005605662493 1105 40-41 0.4546491636207932 1105 42-43 0.4466839508332683 1105 44-45 0.40374288808334313 1105 46-47 0.34023762630072696 1105 48-49 0.1419344586737452 1105 50-51 0.10653773605397987 1105 52-53 0.07333653386343286 1105 54-55 0.18801173442731312 1105 56-57 0.3009554907761398 1105 58-59 0.25406809758251825 1105 60-61 0.10310920213017027 1105 62-63 0.31685672994577274 1105 64-65 0.14689334603262338 1105 66-67 0.2366998263915363 1105 68-69 0.18313024485341245 1105 70-71 0.29004261824234945 1105 72-73 0.27996854308880614 1105 74-75 0.013696712402879285 1105 76-77 0.11281982148874192 1105 78-79 0.16003194398320275 1105 80-81 0.2722756973009375 1105 82-83 0.26717130579039505 1105 84-85 0.2529318050429552 1105 86-87 0.2627844045857479 1105 88-89 0.3274339460723148 1105 90-91 0.318879822356795 1105 92-93 0.39350742614754 1105 94-95 0.24755334849461974 1105 96-97 0.08403043784132791 1105 98-99 -0.20504226558037075 1105 100-101 -0.24019664961159393 1106 1 0.0561589342880815 1106 2 0.07238198297944365 1106 3 0.14841327515733838 1106 4 0.05212187108446642 1106 5 0.013517321027890716 1106 6 0.06853581716493551 1106 7 0.16236847392218579 1106 8 0.09935277790305008 1106 9 -0.01635885701097095 1106 10-11 0.03482140958342228 1106 12-13 0.0019449398212998403 1106 14-15 0.23167041899138496 1106 16-17 -0.0150308615838739 1106 18-19 0.1704482024738283 1106 20-21 0.126496168631256 1106 22-23 0.17662863730284784 1106 24-25 0.06148738562364997 1106 26-27 0.03075292064600177 1106 28-29 0.22926879688876767 1106 30-31 0.17530048604501758 1106 32-33 0.2540498684550485 1106 34-35 0.08761116142554215 1106 36-37 -0.3638061186498618 1106 38-39 -0.020767103087038663 1106 40-41 0.26665756421989073 1106 42-43 0.23414338392577605 1106 44-45 0.24636598019515077 1106 46-47 -0.07815658661989744 1106 48-49 0.20820172554039118 1106 50-51 0.22687904592211794 1106 52-53 0.2260513705665872 1106 54-55 -0.1926188652271108 1106 56-57 0.2485381638298989 1106 58-59 0.3311815538073972 1106 60-61 -0.1788356759295766 1106 62-63 -0.13916058196337033 1106 64-65 -0.41306670940289436 1106 66-67 -0.052343544154009436 1106 68-69 -0.6992243480838276 1106 70-71 -0.4556284875752219 1106 72-73 -0.21291095892676282 1106 74-75 -0.16195541825660342 1106 76-77 -0.21169973658583174 1106 78-79 -0.23006778218587343 1106 80-81 -0.7533644907910642 1106 82-83 -0.1794417499802492 1106 84-85 -0.15166049037352636 1106 86-87 -0.04509521433402952 1106 88-89 0.03355074117315482 1106 90-91 -0.19928055921855048 1106 92-93 -0.13433812550967872 1106 94-95 -0.10133099564753678 1106 96-97 -0.14836585093132726 1106 98-99 -0.43574034876390755 1106 100-101 -0.7994004952558562 1107 1 0.1415330094912406 1107 2 0.19082961800191356 1107 3 0.17518722653944963 1107 4 0.1446140897418715 1107 5 0.10663065936599025 1107 6 0.1412121916524285 1107 7 0.0539711149006834 1107 8 0.09593606009193678 1107 9 0.15100212073660657 1107 10-11 0.16820154512404883 1107 12-13 0.1948986224230964 1107 14-15 0.32271537283604346 1107 16-17 0.2675426689141105 1107 18-19 0.18789327199222328 1107 20-21 0.22167464597258402 1107 22-23 0.2111292055993701 1107 24-25 0.0944170247385685 1107 26-27 0.302544820708178 1107 28-29 0.3388196479010688 1107 30-31 0.3109798835387849 1107 32-33 0.42650816838221317 1107 34-35 0.2841650999062679 1107 36-37 0.3359150825485955 1107 38-39 0.23392028010329113 1107 40-41 0.3327477199094133 1107 42-43 0.30816988234622755 1107 44-45 0.3214463676305783 1107 46-47 0.30504023467983643 1107 48-49 0.3704867565784653 1107 50-51 0.3449192040513509 1107 52-53 0.36533315029493707 1107 54-55 0.3517348994744012 1107 56-57 0.3799887994759459 1107 58-59 0.18960890563509736 1107 60-61 0.1642229367285566 1107 62-63 0.28063384197107055 1107 64-65 0.340497398429747 1107 66-67 0.36244633385584635 1107 68-69 0.489607561934168 1107 70-71 0.2790533581373076 1107 72-73 0.20991643157823603 1107 74-75 0.1866365314056182 1107 76-77 0.15764958515147853 1107 78-79 0.3043600423642694 1107 80-81 0.3550007490685694 1107 82-83 0.44175559865568204 1107 84-85 0.2716314528872452 1107 86-87 0.31729790271983305 1107 88-89 0.12883766265333207 1107 90-91 0.15235275052343056 1107 92-93 0.10871272035460322 1107 94-95 0.1495710268076742 1107 96-97 0.079045872853726 1107 98-99 -0.18836886010454634 1107 100-101 -0.31118084137284185 1108 1 0.162830569117407 1108 2 0.18969361723689104 1108 3 0.07148628034174465 1108 4 0.024902192513863497 1108 5 0.014988781792808936 1108 6 0.024966996495948024 1108 7 0.01341853441581975 1108 8 0.021394174492890272 1108 9 0.10333320625983333 1108 10-11 0.08121754817729254 1108 12-13 0.07315240439633186 1108 14-15 0.13529744579623326 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0.24206326577378334 2316 84-85 0.2503566810013105 2316 86-87 0.1892338176116759 2316 88-89 0.5447312874332475 2316 90-91 0.4079943726437101 2316 92-93 0.2617557379642186 2316 94-95 0.3926703438488097 2316 96-97 0.6504782994757861 2316 98-99 0.7264538146014843 2316 100-101 0.559190318514279 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 17.0 4 56.0 5 152.0 6 235.0 7 662.0 8 1495.0 9 2811.0 10 4521.0 11 6775.0 12 9835.0 13 13525.0 14 17587.0 15 22846.0 16 28460.0 17 34457.0 18 33042.0 19 28878.0 20 28709.0 21 30067.0 22 32777.0 23 36831.0 24 42149.0 25 49662.0 26 58227.0 27 69330.0 28 82792.0 29 97420.0 30 115375.0 31 135734.0 32 161987.0 33 196863.0 34 245806.0 35 311606.0 36 384990.0 37 387605.0 38 162432.0 39 5300.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.251522789662666 20.114832684090793 15.76904353849679 22.86460098774975 2 15.880965477509354 21.041097800694015 39.31399429660875 23.76394242518789 3 19.33059344722305 24.21740419022356 30.45117307533912 26.000829287214263 4 13.283323143334739 17.64443135056024 36.806345324444976 32.265900181660044 5 13.391738616395097 37.69173481164367 34.634722725216626 14.281803846744612 6 32.094623728582086 37.13210849198229 16.911138636426834 13.862129143008797 7 28.302289916195623 32.91207632748931 20.485733825973128 18.299899930341944 8 26.30888684032959 35.67066206128008 19.4529009113273 18.56755018706303 9 26.009557831497904 16.730006571581132 20.00238646599233 37.25804913092863 10-11 24.73065141017965 27.008688073759394 27.495220908257984 20.765439607802968 12-13 25.870576106266025 24.069515853631493 27.787686187465706 22.272221852636783 14-15 22.124106087213015 25.79852454452114 26.204452554204984 25.872916814060858 16-17 21.69436621552604 28.849223571385075 27.274296810900022 22.18211340218886 18-19 21.743925863272395 27.10807135885303 29.75377513928971 21.394227638584862 20-21 22.131814583559926 26.35401146278046 30.10192462498517 21.412249328674445 22-23 21.856244638811187 26.408692508784693 30.005234033820937 21.729828818583176 24-25 21.798365266479728 26.54477002661198 30.095316336933987 21.561548369974307 26-27 21.399669561479886 26.93716370259407 29.739906831614878 21.92325990431117 28-29 21.52965430385766 27.316781537341683 29.670143233717738 21.483420925082918 30-31 21.68503858296014 26.7799146856007 29.538342025630925 21.996704705808227 32-33 21.31186288309731 26.809692712584663 29.614054243175254 22.26439016114277 34-35 22.158542668547422 27.025742607717323 29.318857788171147 21.4968569355641 36-37 22.27700694715523 26.50753127662997 29.38116570146134 21.83429607475346 38-39 21.689230501710195 26.881840034665032 29.335179694625264 22.093749768999505 40-41 22.186196482063593 26.72841418297916 29.405622221361767 21.67976711359548 42-43 21.934435744696128 26.749159499185943 29.153969471116742 22.162435285001187 44-45 21.96660989130316 26.962120308156823 29.36477766267831 21.706492137861705 46-47 22.392337121056126 27.25066094196417 28.48595628121017 21.871045655769528 48-49 22.029034632267766 27.35152256882297 28.39398230424907 22.225460494660194 50-51 22.28433017566572 27.49349617619863 28.37398949180594 21.848184156329715 52-53 22.67223649525998 27.12699061358575 28.349878441588427 21.850894449565843 54-55 22.057334317486564 27.394324082785733 28.54098931334897 22.007352286378733 56-57 22.228628369871252 27.588180446395853 28.14460012791176 22.03859105582113 58-59 22.078330290413202 27.237655054292105 28.212304660894798 22.471709994399898 60-61 21.72703052881341 27.448846735029413 28.51228484407542 22.311837892081755 62-63 21.91187604737874 27.408984305290247 28.656264772418083 22.022874874912926 64-65 21.621135500230377 27.499726330780376 28.42905772255819 22.450080446431052 66-67 21.527211788862488 27.623225527587707 28.34863387997376 22.50092880357604 68-69 22.163281677199056 27.109535608695325 28.471134356459533 22.256048357646087 70-71 21.91644198210502 27.282296499144675 28.05606789110953 22.745193627640777 72-73 21.87724061173782 27.363102912650067 28.13245660626937 22.627199869342746 74-75 21.90013730979109 27.700076838573175 27.866812670515284 22.53297318112045 76-77 21.91914456105745 27.274613598421134 28.261494723199863 22.544747117321553 78-79 21.914339950320677 27.506502063870702 28.204543366627703 22.37461461918092 80-81 22.133088677092832 27.46282725793411 27.918161009196435 22.485923055776624 82-83 22.062891058384913 27.57832783293817 28.015813401440614 22.342967707236305 84-85 21.94035206967966 27.698625059466476 27.914050772531745 22.446972098322128 86-87 22.14763671435008 27.63817512390178 27.58956479499887 22.62462336674927 88-89 21.96210405676982 27.769356496169596 27.456968306942255 22.811571140118332 90-91 21.807625401013613 27.683104030164813 27.713393449882183 22.79587711893939 92-93 21.93728117277139 27.322080945527222 27.841247280444154 22.899390601257235 94-95 21.675884603376463 27.13241270121742 28.00336780570633 23.18833488969979 96-97 21.894839918465934 26.746370406976926 28.030486223086697 23.32830345147044 98-99 21.53826794601939 27.094775819951987 27.655085145475923 23.7118710885527 100-101 21.682107130537624 26.795693096141527 28.072382454188986 23.44981731913186 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 706.0 1 475.5 2 349.5 3 466.5 4 405.0 5 397.5 6 797.5 7 1865.5 8 2816.0 9 3021.5 10 2623.0 11 2104.5 12 2023.0 13 2189.0 14 2420.5 15 2663.0 16 2955.5 17 3269.0 18 3689.0 19 4230.5 20 4892.5 21 5816.0 22 7145.5 23 9089.5 24 11561.5 25 14661.5 26 18710.0 27 23660.5 28 28465.5 29 33220.0 30 38340.5 31 45421.5 32 55900.0 33 60577.5 34 60995.5 35 64939.0 36 70459.0 37 75796.0 38 79435.5 39 82109.0 40 85837.5 41 90522.0 42 92843.5 43 94756.5 44 97329.5 45 101441.5 46 103522.5 47 104348.5 48 104601.0 49 101941.0 50 99332.0 51 96063.0 52 93270.0 53 92963.5 54 94005.5 55 88489.5 56 79627.5 57 71699.0 58 64987.5 59 56448.0 60 44132.0 61 36294.5 62 31142.5 63 22847.5 64 15135.0 65 10999.0 66 8239.0 67 6508.0 68 5185.5 69 4051.5 70 3159.0 71 2262.0 72 1727.0 73 1459.5 74 1094.5 75 765.0 76 564.5 77 401.5 78 285.0 79 188.5 80 106.0 81 59.0 82 34.0 83 20.5 84 8.0 85 5.5 86 2.5 87 2.0 88 1.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.335750422119372E-4 2 0.04600458778727788 3 9.503625633179057E-4 4 0.0 5 0.08676458216957918 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.639896009216405E-4 26-27 0.008817252670782793 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011333953532902431 36-37 7.039722691243747E-5 38-39 0.00415343638783381 40-41 0.005314990631889028 42-43 2.2879098746542175E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.7599306728109368E-5 68-69 7.919688027649216E-4 70-71 3.5198613456218736E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.855611767741245E-4 82-83 4.575819749308435E-4 84-85 0.00473421350986142 86-87 0.002147115420829343 88-89 1.583937605529843E-4 90-91 0.0033438682783407795 92-93 8.447667229492497E-4 94-95 7.039722691243747E-5 96-97 0.0 98-99 3.5198613456218736E-5 100-101 3.5198613456218736E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2841021.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.19739430714111 #Duplication Level Percentage of deduplicated Percentage of total 1 74.34230775024866 40.29159366843059 2 14.53033648680426 15.750127519815399 3 5.016259271329505 8.156045450252927 4 2.2081752579083305 4.787093806085232 5 1.1989735681148872 3.2490621617481223 6 0.6600187988543911 2.146277945498225 7 0.4267805891564646 1.6191277111202833 8 0.28909994213578755 1.2534770846483967 9 0.20285057467428713 0.9894575322947262 >10 1.0109777516140797 9.84046469087925 >50 0.06862784159112724 2.558517034684658 >100 0.03922618850738718 4.142800225055829 >500 0.003053071590480802 1.1600399773069898 >1k 0.0031180305603882076 3.211321594155138 >5k 1.9487691002426297E-4 0.8445935980242477 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9757 0.34343287149232615 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8118 0.2857423440375837 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6115 0.21523952128477755 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4063 0.14301196647261671 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3952 0.13910492037897643 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3847 0.13540906596607347 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3698 0.13016447256109687 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3425 0.12055525108754916 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3242 0.11411390482506115 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.519861345621873E-5 0.0 5 0.0 0.0 0.0 3.519861345621873E-5 0.0 6 0.0 0.0 0.0 3.519861345621873E-5 0.0 7 0.0 0.0 0.0 3.519861345621873E-5 0.0 8 0.0 0.0 0.0 3.519861345621873E-5 0.0 9 0.0 0.0 0.0 3.519861345621873E-5 0.0 10-11 0.0 0.0 0.0 7.039722691243746E-5 0.0 12-13 0.0 0.0 0.0 7.039722691243746E-5 3.519861345621873E-5 14-15 0.0 0.0 0.0 7.039722691243746E-5 3.519861345621873E-5 16-17 0.0 0.0 0.0 7.039722691243746E-5 3.519861345621873E-5 18-19 0.0 0.0 0.0 1.055958403686562E-4 3.519861345621873E-5 20-21 0.0 0.0 0.0 1.4079445382487492E-4 3.519861345621873E-5 22-23 0.0 0.0 0.0 1.7599306728109367E-4 3.519861345621873E-5 24-25 0.0 0.0 0.0 4.223833614746248E-4 3.519861345621873E-5 26-27 0.0 0.0 0.0 7.919688027649215E-4 3.519861345621873E-5 28-29 0.0 0.0 0.0 0.0021471154208293425 3.519861345621873E-5 30-31 0.0 0.0 0.0 0.007602900506543247 3.519861345621873E-5 32-33 0.0 0.0 0.0 0.019693624228754383 3.519861345621873E-5 34-35 0.0 0.0 0.0 0.03993282696608015 3.519861345621873E-5 36-37 0.0 0.0 0.0 0.07361790004368149 3.519861345621873E-5 38-39 0.0 0.0 0.0 0.12414550966008348 3.519861345621873E-5 40-41 0.0 0.0 0.0 0.18060408564385833 3.519861345621873E-5 42-43 0.0 0.0 0.0 0.2425536453268033 3.519861345621873E-5 44-45 0.0 0.0 0.0 0.31916342751426335 3.519861345621873E-5 46-47 0.0 0.0 0.0 0.4141292866191415 3.519861345621873E-5 48-49 0.0 0.0 0.0 0.5107846791699181 3.519861345621873E-5 50-51 0.0 0.0 0.0 0.6173660807153485 3.519861345621873E-5 52-53 0.0 0.0 0.0 0.723665893353129 7.039722691243746E-5 54-55 0.0 0.0 0.0 0.8414932518978212 7.039722691243746E-5 56-57 0.0 0.0 0.0 0.9827804863110832 7.039722691243746E-5 58-59 0.0 0.0 0.0 1.1319522101385382 7.039722691243746E-5 60-61 0.0 0.0 0.0 1.2972097003154852 7.039722691243746E-5 62-63 0.0 0.0 0.0 1.457627381142202 1.055958403686562E-4 64-65 0.0 0.0 0.0 1.6288862349134343 1.055958403686562E-4 66-67 0.0 0.0 0.0 1.8317182449548948 1.055958403686562E-4 68-69 0.0 0.0 0.0 2.057851737104372 1.055958403686562E-4 70-71 0.0 0.0 0.0 2.2962871446567976 1.055958403686562E-4 72-73 0.0 0.0 0.0 2.5498931546088537 1.055958403686562E-4 74-75 0.0 0.0 0.0 2.8073182141209094 1.055958403686562E-4 76-77 0.0 0.0 0.0 3.0898926829474336 1.055958403686562E-4 78-79 0.0 0.0 0.0 3.403917112897089 1.055958403686562E-4 80-81 0.0 0.0 0.0 3.7395710908155904 1.055958403686562E-4 82-83 0.0 0.0 0.0 4.088618141154184 1.055958403686562E-4 84-85 0.0 0.0 0.0 4.445954464961716 1.2319514709676556E-4 86-87 0.0 0.0 0.0 4.818584586315976 1.4079445382487492E-4 88-89 0.0 0.0 0.0 5.221995895137699 1.4079445382487492E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4975 0.0 56.968433 1 GTATCAA 9200 0.0 42.48788 1 TGGTATC 1915 0.0 34.268208 2 ATCAACG 12085 0.0 31.74183 3 TCAACGC 12265 0.0 31.27544 4 TATCAAC 12395 0.0 31.152332 2 CAACGCA 12570 0.0 30.554386 5 AACGCAG 13025 0.0 29.4642 6 GTGGTAT 2440 0.0 28.259163 1 CGCAGAG 14810 0.0 25.816765 8 ACGCAGA 14865 0.0 25.753197 7 TAAGGTG 2090 0.0 24.10777 5 TACATGG 9295 0.0 23.482502 2 GTAAGGT 2120 0.0 23.318195 4 GTACATG 9810 0.0 23.219217 1 GCAGAGT 16765 0.0 22.494583 9 ACATGGG 9295 0.0 22.194504 3 GGTAAGG 2160 0.0 22.00652 3 AGGTAAG 2140 0.0 21.332344 2 AGAGTAC 15385 0.0 21.008337 10-11 >>END_MODULE