FastQCFastQC Report
Thu 26 May 2016
SRR938309_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938309_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2848133
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104550.36708257655102483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78970.27726935504767514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58550.20557326501255382No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA43120.15139742420736674No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA41900.14711391637960727No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA39550.1388628972031854No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA37210.13064698874666317No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC33080.11614626142810043No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA32830.11526849343060876No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG30770.10803568513127722No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA28560.10027621603345069No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47750.054.900191
GTATCAA85850.043.3050771
ATCAACG110950.033.1095123
TATCAAC111950.032.813762
TCAACGC112100.032.6850624
CAACGCA114650.031.9580945
AACGCAG119050.030.8125346
ACGCAGA136300.026.8045777
CGCAGAG137600.026.3787028
TGGTATC21500.025.6401212
GTGGTAT23900.025.1279541
TAAGGTG18800.024.266885
TACATGG92500.024.146642
GTACATG97050.023.9176181
AGGTAAG17700.023.3585852
ACATGGG93050.023.2378313
GCAGAGT155400.022.7147859
GTAAGGT20300.021.7714544
GTGTAAG12750.021.308321
GAGTACT102350.020.60834312-13
Produced by FastQC (version 0.11.5)