##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938309_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2848133 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.420193158114458 31.0 31.0 34.0 30.0 34.0 2 31.595203945883146 33.0 31.0 34.0 30.0 34.0 3 31.65638016202193 33.0 31.0 34.0 30.0 34.0 4 35.32762830949257 37.0 35.0 37.0 33.0 37.0 5 35.34042090028802 37.0 35.0 37.0 33.0 37.0 6 35.355317676527044 37.0 35.0 37.0 33.0 37.0 7 35.28431537431714 37.0 35.0 37.0 32.0 37.0 8 35.241103909122224 37.0 35.0 37.0 32.0 37.0 9 36.78589763890942 39.0 37.0 39.0 33.0 39.0 10-11 36.663739544466495 39.0 37.0 39.0 32.0 39.0 12-13 36.51569852952794 39.0 37.0 39.0 32.0 39.0 14-15 37.571129929676744 40.0 37.0 41.0 32.0 41.0 16-17 37.415809935842184 39.5 37.0 41.0 32.0 41.0 18-19 37.30222903916355 39.0 37.0 41.0 32.0 41.0 20-21 37.28898650449259 39.0 36.5 41.0 31.5 41.0 22-23 37.39343071408533 40.0 37.0 41.0 32.0 41.0 24-25 37.32004334067264 40.0 37.0 41.0 31.5 41.0 26-27 37.138985784722834 39.0 36.0 41.0 31.0 41.0 28-29 36.96326856926976 39.0 36.0 41.0 31.0 41.0 30-31 36.70014163664408 39.0 36.0 40.5 30.0 41.0 32-33 36.400496044250744 39.0 35.0 40.0 30.0 41.0 34-35 36.154289318651905 38.5 35.0 40.0 30.0 41.0 36-37 35.86928050761674 38.0 35.0 40.0 28.5 41.0 38-39 35.548864291098766 38.0 35.0 40.0 27.0 41.0 40-41 35.43850743627492 38.0 35.0 40.0 27.0 41.0 42-43 35.21348125245555 38.0 34.0 40.0 26.0 41.0 44-45 34.922276452679704 38.0 34.0 40.0 25.5 41.0 46-47 34.535816972030446 38.0 33.5 40.0 24.0 41.0 48-49 34.15005847690399 38.0 33.0 40.0 23.0 41.0 50-51 33.3717456312609 37.5 32.0 39.5 22.0 40.5 52-53 33.39962494729003 37.5 33.0 39.5 22.0 40.5 54-55 33.496941856296736 38.0 33.0 40.0 20.5 41.0 56-57 33.18555629249056 37.0 32.0 40.0 18.5 41.0 58-59 32.7758263044598 37.0 31.5 40.0 16.0 41.0 60-61 32.313630718790165 36.0 31.0 39.0 12.0 41.0 62-63 31.74641247441745 36.0 30.5 39.0 8.0 40.5 64-65 31.27441625794863 35.0 30.0 39.0 7.0 40.0 66-67 30.742298375813206 35.0 29.0 38.0 2.0 40.0 68-69 30.141371909247216 34.5 28.5 38.0 2.0 40.0 70-71 29.512798559617828 34.0 28.0 37.0 2.0 39.5 72-73 28.890643976246896 34.0 26.5 36.5 2.0 39.0 74-75 28.215497661099395 34.0 26.0 36.0 2.0 39.0 76-77 27.497692347934596 33.0 25.5 35.5 2.0 38.0 78-79 26.72552177163075 32.5 23.5 35.0 2.0 37.0 80-81 25.994777280414922 32.0 21.0 35.0 2.0 37.0 82-83 25.27014925215922 31.5 19.0 35.0 2.0 36.0 84-85 24.551617849306897 31.0 14.5 34.5 2.0 36.0 86-87 23.844832211136207 31.0 7.0 34.0 2.0 35.0 88-89 23.078569013455482 30.0 2.0 34.0 2.0 35.0 90-91 22.263481375343076 29.5 2.0 34.0 2.0 35.0 92-93 21.416952438667714 29.0 2.0 34.0 2.0 35.0 94-95 20.518533720159837 29.0 2.0 34.0 2.0 35.0 96-97 19.448612652569246 26.5 2.0 34.0 2.0 35.0 98-99 18.188393765319248 24.5 2.0 33.5 2.0 35.0 100-101 15.2543743568155 10.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4760691347704977 1101 2 0.46611226074278633 1101 3 0.5003063051896959 1101 4 0.33376377276616154 1101 5 0.40934870211876273 1101 6 0.4408625561296162 1101 7 0.4085508131017974 1101 8 0.3629762587360261 1101 9 0.4667006624787291 1101 10-11 0.5533809157187122 1101 12-13 0.5071475297516983 1101 14-15 0.5934177366070088 1101 16-17 0.749794334924438 1101 18-19 0.6944252365333412 1101 20-21 0.7755530875242727 1101 22-23 0.8481608878807947 1101 24-25 0.8759265903399225 1101 26-27 0.8293815379656095 1101 28-29 0.9321643128159707 1101 30-31 0.9752052330230967 1101 32-33 1.0506196252047673 1101 34-35 1.064830046087458 1101 36-37 1.0815717713407977 1101 38-39 1.288454131636371 1101 40-41 1.4389942560450493 1101 42-43 1.4122130407184699 1101 44-45 1.445192293547258 1101 46-47 1.5510865120292436 1101 48-49 1.5334537988158985 1101 50-51 1.5785394985425327 1101 52-53 1.7533293084792163 1101 54-55 1.9319447960856522 1101 56-57 2.0123569468399225 1101 58-59 2.15886189129467 1101 60-61 2.1660043156018105 1101 62-63 2.141860220616646 1101 64-65 2.1957412534940097 1101 66-67 2.3872606666046003 1101 68-69 2.4599154683256543 1101 70-71 2.6643274216944874 1101 72-73 2.772168915001366 1101 74-75 2.947496197016495 1101 76-77 3.1575337427267947 1101 78-79 3.4671974109854737 1101 80-81 3.763821431478693 1101 82-83 3.89877228655822 1101 84-85 4.1172081154806115 1101 86-87 4.4824347053639855 1101 88-89 4.683308982125329 1101 90-91 4.856278977039178 1101 92-93 4.895155873408232 1101 94-95 4.889626728145384 1101 96-97 5.1358974038976015 1101 98-99 5.450148938693008 1101 100-101 5.609996102836639 1102 1 0.5419786092363559 1102 2 0.4646271289373409 1102 3 0.42437556633662155 1102 4 0.3936314610234888 1102 5 0.36026847124826844 1102 6 0.3603295963294286 1102 7 0.4020499861534077 1102 8 0.32732972010572325 1102 9 0.5136747064251281 1102 10-11 0.5727753869780372 1102 12-13 0.5731970791492103 1102 14-15 0.7814134837295654 1102 16-17 0.7508212698149137 1102 18-19 0.748408090011587 1102 20-21 0.8359937328815477 1102 22-23 0.5793174342742873 1102 24-25 0.46402528409898736 1102 26-27 0.577071145418266 1102 28-29 0.6609623686434247 1102 30-31 0.7330882451404221 1102 32-33 0.722501692238211 1102 34-35 0.669451675007025 1102 36-37 0.8975538147471482 1102 38-39 0.8067490990647315 1102 40-41 1.0198293051556675 1102 42-43 1.279515670076826 1102 44-45 1.2167165788275653 1102 46-47 1.2077712928035282 1102 48-49 1.1283284064373262 1102 50-51 1.2588480009221215 1102 52-53 1.2349583968108035 1102 54-55 1.3852610279012296 1102 56-57 1.4251780222103605 1102 58-59 1.5262995326353348 1102 60-61 1.512763184201404 1102 62-63 1.7147709101903494 1102 64-65 1.9650985964582013 1102 66-67 2.0447656451714487 1102 68-69 2.058693609885708 1102 70-71 2.1627351241280763 1102 72-73 2.1169329815622753 1102 74-75 2.382703102879386 1102 76-77 2.5533728421968718 1102 78-79 2.85497460746166 1102 80-81 3.1909596837485807 1102 82-83 3.4530968891167007 1102 84-85 3.4204374333325696 1102 86-87 3.775614068782488 1102 88-89 4.072400013954404 1102 90-91 4.360827193193362 1102 92-93 4.33110450134896 1102 94-95 4.672461642244009 1102 96-97 4.9515140711268195 1102 98-99 5.199363337720921 1102 100-101 5.278972036950794 1103 1 0.4995108998601765 1103 2 0.4151941637800327 1103 3 0.3713532952430896 1103 4 0.3541097069081829 1103 5 0.3054819093996244 1103 6 0.2770731258956616 1103 7 0.3332721883165135 1103 8 0.34912158303264107 1103 9 0.4549098740737847 1103 10-11 0.47607502099027954 1103 12-13 0.56915069881736 1103 14-15 0.658130589964486 1103 16-17 0.7414106249871537 1103 18-19 0.8039044711640386 1103 20-21 0.7488640287900381 1103 22-23 0.5821640968926616 1103 24-25 0.7312447618689291 1103 26-27 0.7200254708027174 1103 28-29 0.8843453756513497 1103 30-31 0.8610789231204379 1103 32-33 0.8762444504401117 1103 34-35 0.8906362936740422 1103 36-37 0.9338440446793683 1103 38-39 0.9536866185539878 1103 40-41 1.0005213994918734 1103 42-43 1.2589744020851583 1103 44-45 1.1263217059385653 1103 46-47 1.2781041283059835 1103 48-49 1.1694273544276683 1103 50-51 1.2808770173505408 1103 52-53 1.3723506930147096 1103 54-55 1.5141740606760052 1103 56-57 1.5090647137138617 1103 58-59 1.8352826446801132 1103 60-61 1.954424243432328 1103 62-63 1.8878273182584913 1103 64-65 2.0301475862991616 1103 66-67 2.0892553138130303 1103 68-69 2.0931793396456086 1103 70-71 2.4404738778002653 1103 72-73 2.4465623984436675 1103 74-75 2.668670016273225 1103 76-77 2.9234972452101182 1103 78-79 2.990724061762428 1103 80-81 3.2506608211006665 1103 82-83 3.3937174271022883 1103 84-85 3.791185492612737 1103 86-87 3.790434931725816 1103 88-89 4.150499831781126 1103 90-91 4.278094265783668 1103 92-93 4.091681219275795 1103 94-95 4.313602440852268 1103 96-97 4.699163871720934 1103 98-99 4.839919048278766 1103 100-101 4.958823015927013 1104 1 0.5341282690001421 1104 2 0.470287712332631 1104 3 0.41575751676249695 1104 4 0.3195964043010946 1104 5 0.2736457788546929 1104 6 0.36317148280611633 1104 7 0.34837732455654447 1104 8 0.36745084567043307 1104 9 0.4962528454201518 1104 10-11 0.6363643453129058 1104 12-13 0.555275967817245 1104 14-15 0.6315248917651815 1104 16-17 0.7416671610914634 1104 18-19 0.8537419337605954 1104 20-21 0.872934085411913 1104 22-23 0.7659812912008519 1104 24-25 0.8560691798465925 1104 26-27 0.9270328028950061 1104 28-29 0.8412423488157401 1104 30-31 0.9000652715274455 1104 32-33 0.9375666230008193 1104 34-35 0.9918840756048013 1104 36-37 1.0587152925968226 1104 38-39 1.0916663773713253 1104 40-41 1.184636635509932 1104 42-43 1.222035381201188 1104 44-45 1.2866343847272859 1104 46-47 1.4356441600390184 1104 48-49 1.477176535690269 1104 50-51 1.5121635555942632 1104 52-53 1.6485495636750116 1104 54-55 1.7377862971577613 1104 56-57 2.0120147579058383 1104 58-59 2.1505137256264533 1104 60-61 2.1456969577682514 1104 62-63 2.2694424405294065 1104 64-65 2.423776705760769 1104 66-67 2.3549607687386676 1104 68-69 2.6546640254082376 1104 70-71 2.8174254883145693 1104 72-73 2.9581278204746013 1104 74-75 2.96854741137658 1104 76-77 2.9729662396757135 1104 78-79 3.2338514353264323 1104 80-81 3.62281097119849 1104 82-83 3.7141231558923167 1104 84-85 3.9704110536765604 1104 86-87 4.0059285265517275 1104 88-89 4.32854665784868 1104 90-91 4.75045225852902 1104 92-93 4.7221864598727805 1104 94-95 4.935050504875935 1104 96-97 5.180925485788016 1104 98-99 5.200220860585333 1104 100-101 5.140772700000383 1105 1 0.4705604924694029 1105 2 0.4808377492784679 1105 3 0.439026413580315 1105 4 0.46491219348089885 1105 5 0.3504437940164422 1105 6 0.4221866389743951 1105 7 0.4642634755449606 1105 8 0.4710690248828584 1105 9 0.5230809197883062 1105 10-11 0.47457345874313006 1105 12-13 0.5840169430756319 1105 14-15 0.6540502187404797 1105 16-17 0.8245437899188985 1105 18-19 0.8780687192123082 1105 20-21 0.8545407360345934 1105 22-23 0.7795167750237866 1105 24-25 0.7601921769076725 1105 26-27 0.9182218725113742 1105 28-29 0.9498528689796046 1105 30-31 0.8320472434486064 1105 32-33 0.8197529137692001 1105 34-35 0.9906263351264997 1105 36-37 1.116076727100328 1105 38-39 1.2456116249456528 1105 40-41 1.1592289284410882 1105 42-43 1.283966885296877 1105 44-45 1.2822595138515922 1105 46-47 1.3573769197347403 1105 48-49 1.4715388058955554 1105 50-51 1.2716322056101816 1105 52-53 1.405213540833742 1105 54-55 1.5321485208317611 1105 56-57 1.7422764219016074 1105 58-59 1.6021414363061268 1105 60-61 1.9219203581624598 1105 62-63 2.227557542163023 1105 64-65 2.301148870377265 1105 66-67 2.3269065951621037 1105 68-69 2.32126694422233 1105 70-71 2.685806646496715 1105 72-73 2.7796680337367548 1105 74-75 2.8698375841565245 1105 76-77 2.899684825943776 1105 78-79 3.1675962447622297 1105 80-81 3.524532488937144 1105 82-83 3.7151819751518005 1105 84-85 3.850630001957974 1105 86-87 4.138849080906777 1105 88-89 4.403395710378771 1105 90-91 4.586567697049102 1105 92-93 4.615422300869813 1105 94-95 4.935198939656768 1105 96-97 5.142838470434558 1105 98-99 5.307900522507673 1105 100-101 5.00432902983642 1106 1 0.1829622882045321 1106 2 0.34305863139984893 1106 3 0.24154626847717608 1106 4 0.2297472959334712 1106 5 0.29250723290132896 1106 6 0.05590124960995979 1106 7 0.21600421070194642 1106 8 0.1020187469049958 1106 9 0.3055806917850319 1106 10-11 0.19174843310440082 1106 12-13 0.16998790197362013 1106 14-15 0.39318469972128867 1106 16-17 0.13381393749750004 1106 18-19 0.3676308226494811 1106 20-21 0.431918996248676 1106 22-23 0.4033269702131932 1106 24-25 0.4709525817670297 1106 26-27 0.467156259685126 1106 28-29 0.29580339133974576 1106 30-31 0.46402274752195183 1106 32-33 0.6882524940570605 1106 34-35 0.5553271483071356 1106 36-37 0.5609443735296082 1106 38-39 0.5085045118020588 1106 40-41 0.5505488440147346 1106 42-43 0.3772137076758142 1106 44-45 0.6444876083212563 1106 46-47 0.9232984810266771 1106 48-49 0.9181916248535131 1106 50-51 0.4843857778164846 1106 52-53 0.856025558188044 1106 54-55 0.8825050900247007 1106 56-57 0.9243947304709934 1106 58-59 0.27036969270464084 1106 60-61 1.3226516902785335 1106 62-63 1.5604232361398367 1106 64-65 1.6898946755824404 1106 66-67 1.8280403297811851 1106 68-69 1.4086077839816298 1106 70-71 1.6884817297411772 1106 72-73 2.1265091647818686 1106 74-75 2.2658725142572393 1106 76-77 2.3395643192367537 1106 78-79 2.6323425190027017 1106 80-81 3.1773377201696817 1106 82-83 3.4605223328470487 1106 84-85 3.4625235365297833 1106 86-87 3.7909079504200385 1106 88-89 3.870384792279406 1106 90-91 4.397571600092807 1106 92-93 4.58141142557923 1106 94-95 4.6420120960967886 1106 96-97 4.8966319329759465 1106 98-99 5.207936909968051 1106 100-101 4.747940052538105 1107 1 0.37141688245622717 1107 2 0.24561072235773906 1107 3 0.2678614872239571 1107 4 0.22925254704902898 1107 5 0.33178216146772144 1107 6 0.20594570923573485 1107 7 0.3051037406227337 1107 8 0.328830612203177 1107 9 0.30309881450444465 1107 10-11 0.37813863680539583 1107 12-13 0.3775689459073206 1107 14-15 0.567548439101131 1107 16-17 0.5742021188277064 1107 18-19 0.5502365362535855 1107 20-21 0.5504411745534625 1107 22-23 0.5609038121700678 1107 24-25 0.5709752182228343 1107 26-27 0.5626455053354036 1107 28-29 0.6028330274756613 1107 30-31 0.7171965955025641 1107 32-33 0.7642940421195448 1107 34-35 0.8210493190212489 1107 36-37 0.8832807268643563 1107 38-39 1.0167288648331692 1107 40-41 0.8866270170494275 1107 42-43 0.8962046097182395 1107 44-45 0.8482733183469051 1107 46-47 0.8122517550443575 1107 48-49 1.0055608891551415 1107 50-51 1.103871699838642 1107 52-53 1.3641441840602937 1107 54-55 1.4444889923870292 1107 56-57 1.5081997695900426 1107 58-59 1.6423185153378483 1107 60-61 1.601732758610808 1107 62-63 1.8560160401867414 1107 64-65 1.8589625686841522 1107 66-67 1.9160974983536612 1107 68-69 2.1383745878225113 1107 70-71 2.137980067927117 1107 72-73 2.3655725034828876 1107 74-75 2.440924835646115 1107 76-77 2.6988387598316486 1107 78-79 2.832805278644205 1107 80-81 3.098158921989274 1107 82-83 3.29254220955292 1107 84-85 3.3080907220728157 1107 86-87 3.5826945767477305 1107 88-89 3.6185108461623052 1107 90-91 3.852891709398424 1107 92-93 4.0114559665335925 1107 94-95 4.161134479349606 1107 96-97 4.447500928893252 1107 98-99 4.71957977446921 1107 100-101 4.614789572970555 1108 1 0.1789319877274771 1108 2 0.2838880427633299 1108 3 0.1989581785155039 1108 4 0.10650270900973169 1108 5 0.14572581155894682 1108 6 0.17115455826352388 1108 7 0.17695235778016638 1108 8 0.06121800080568107 1108 9 0.11021688589389811 1108 10-11 0.2247318206253368 1108 12-13 0.26150638304451945 1108 14-15 0.20231371703768986 1108 16-17 0.46426698018377266 1108 18-19 0.4612906167856252 1108 20-21 0.447103338862334 1108 22-23 0.3930055804427255 1108 24-25 0.3482956773391592 1108 26-27 0.3643254756879202 1108 28-29 0.4769629570008078 1108 30-31 0.5532353577416771 1108 32-33 0.5662919928295338 1108 34-35 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18-19 -0.1296083077260164 2311 20-21 0.04601221471565964 2311 22-23 -0.05086693357941385 2311 24-25 -0.03958637925422437 2311 26-27 0.12355770015069822 2311 28-29 0.015268493729521992 2311 30-31 0.19422998148443327 2311 32-33 0.1884041901902762 2311 34-35 0.16981443583028266 2311 36-37 0.21766113741815474 2311 38-39 0.35959754209764583 2311 40-41 0.21815432225639597 2311 42-43 0.43065403962103943 2311 44-45 0.4533061647467562 2311 46-47 0.24758867947930696 2311 48-49 0.3510246677484048 2311 50-51 0.44847470106710574 2311 52-53 0.45656430103958456 2311 54-55 0.5515344665774577 2311 56-57 0.5685687808191133 2311 58-59 0.696256416259061 2311 60-61 0.7310418840950632 2311 62-63 0.9123804230594708 2311 64-65 0.871644710353241 2311 66-67 0.8845149252808966 2311 68-69 0.8631095116130965 2311 70-71 1.2357151331615341 2311 72-73 1.181297717416676 2311 74-75 1.3428986973507229 2311 76-77 1.5263083952470886 2311 78-79 1.8030231676212765 2311 80-81 1.824445471467115 2311 82-83 2.093279289052205 2311 84-85 2.3125386720540853 2311 86-87 2.486562214138143 2311 88-89 2.346623723575032 2311 90-91 2.603030206479179 2311 92-93 2.865622142075857 2311 94-95 3.0083050789404666 2311 96-97 2.9201400611615647 2311 98-99 2.9137919716419063 2311 100-101 2.778611647113312 2312 1 -0.24733378958417163 2312 2 -0.26619516516958086 2312 3 -0.15754267625254315 2312 4 -0.16580000489079083 2312 5 -0.15168532084546626 2312 6 -0.04395065079913962 2312 7 -0.18421065509797074 2312 8 -0.17774987783248974 2312 9 -0.21715016755677397 2312 10-11 -0.17579803010742268 2312 12-13 -0.13518071305241364 2312 14-15 -0.06064950157276883 2312 16-17 -0.014265927027793168 2312 18-19 0.13817812347374314 2312 20-21 0.0045181440690669206 2312 22-23 -0.05932340549846771 2312 24-25 -0.11227756742510309 2312 26-27 -0.016736392390093613 2312 28-29 0.06824594205171763 2312 30-31 0.10365820402073211 2312 32-33 0.10910874033939422 2312 34-35 -0.18948633118350244 2312 36-37 0.040480530466616926 2312 38-39 0.19533760841883208 2312 40-41 0.19828729042575333 2312 42-43 0.16656176599306605 2312 44-45 0.2058093364735285 2312 46-47 0.2433271297999937 2312 48-49 0.3798344377745977 2312 50-51 0.5725672483725859 2312 52-53 0.4888235479181944 2312 54-55 0.6423849715450345 2312 56-57 0.6676300995667361 2312 58-59 0.8081699889038276 2312 60-61 0.9408142235098964 2312 62-63 1.0358729976023788 2312 64-65 1.0602695981943349 2312 66-67 1.1902197002365362 2312 68-69 1.269520113879537 2312 70-71 1.376272314491775 2312 72-73 1.4084932137275672 2312 74-75 1.4646446229769055 2312 76-77 1.4888520451796765 2312 78-79 1.658294847929465 2312 80-81 1.8970410354154446 2312 82-83 2.136739602230495 2312 84-85 2.444979197697343 2312 86-87 2.801755941971077 2312 88-89 2.843233706418058 2312 90-91 2.951843423538673 2312 92-93 3.1707279216885773 2312 94-95 3.1989196673848888 2312 96-97 3.3330234621649204 2312 98-99 3.5154028895497333 2312 100-101 3.575836492078267 2313 1 -0.4373191981808944 2313 2 -0.46557108404880054 2313 3 -0.4299511235781601 2313 4 -0.4009012642821972 2313 5 -0.25442793832123556 2313 6 -0.3665687580047816 2313 7 -0.40727685476161923 2313 8 -0.3105017032225703 2313 9 -0.34493837706497743 2313 10-11 -0.47387699684207973 2313 12-13 -0.44437769113796577 2313 14-15 -0.49215632190647085 2313 16-17 -0.4183778189726439 2313 18-19 -0.29230228619219645 2313 20-21 -0.22906522531151552 2313 22-23 -0.43468928908556137 2313 24-25 -0.2993974236136907 2313 26-27 -0.273602700569775 2313 28-29 -0.2827925277615293 2313 30-31 -0.21481080853217094 2313 32-33 -0.14154165608263014 2313 34-35 -0.10789994652833457 2313 36-37 -0.043768793423801355 2313 38-39 -0.11218052830811587 2313 40-41 0.051017783659446536 2313 42-43 0.009789620047783387 2313 44-45 0.03700473466455634 2313 46-47 0.2247936390630798 2313 48-49 0.2488880666334765 2313 50-51 0.24803253447052498 2313 52-53 0.30161629954178437 2313 54-55 0.30040404289643874 2313 56-57 0.3963421274983929 2313 58-59 0.6120262790419773 2313 60-61 0.7515051686051493 2313 62-63 0.852790758136063 2313 64-65 0.7913731125320247 2313 66-67 0.7774524013842203 2313 68-69 0.9213520787195577 2313 70-71 0.8907011390066373 2313 72-73 1.1299051459997642 2313 74-75 1.1491773774712577 2313 76-77 1.2339825752580502 2313 78-79 1.3625740055924567 2313 80-81 1.6301386214056137 2313 82-83 1.6668731446541116 2313 84-85 1.8644804145155724 2313 86-87 2.1166713310355654 2313 88-89 2.2927480369467084 2313 90-91 2.3214777140849847 2313 92-93 2.5159449651506485 2313 94-95 2.680239872035589 2313 96-97 2.830245301944604 2313 98-99 2.8978133646344943 2313 100-101 2.9068603607237407 2314 1 -0.3430699410240443 2314 2 -0.3219168463610167 2314 3 -0.43038254589202296 2314 4 -0.307476308768571 2314 5 -0.2315311382958427 2314 6 -0.269875422183965 2314 7 -0.14199229417431525 2314 8 -0.11446819738509362 2314 9 -0.12141497823448333 2314 10-11 -0.28938306622851684 2314 12-13 -0.33159557430543884 2314 14-15 -0.27532952371429786 2314 16-17 -0.2763627917431606 2314 18-19 -0.24457117241654203 2314 20-21 -0.27334301723251997 2314 22-23 -0.3282437684605384 2314 24-25 -0.3075989820458247 2314 26-27 -0.19693973273405874 2314 28-29 -0.09026886269293755 2314 30-31 -0.06976076419154253 2314 32-33 0.043993744838900284 2314 34-35 -0.1337088471722936 2314 36-37 -0.0031402771861905876 2314 38-39 0.2125444399667913 2314 40-41 0.29652623040308157 2314 42-43 0.47047707142370143 2314 44-45 0.35355211241365936 2314 46-47 0.35266986879481266 2314 48-49 0.4309741943331886 2314 50-51 0.44486329708396255 2314 52-53 0.4523025266111347 2314 54-55 0.4689708139206701 2314 56-57 0.5326106872409824 2314 58-59 0.930487364554935 2314 60-61 0.9010070896004478 2314 62-63 1.0076752554999153 2314 64-65 0.8638143280028565 2314 66-67 1.0968521219313914 2314 68-69 1.3219199237651011 2314 70-71 1.357829803262259 2314 72-73 1.4487108511388058 2314 74-75 1.6378387763807822 2314 76-77 1.7412918150154937 2314 78-79 2.038818765851854 2314 80-81 2.3024246284884526 2314 82-83 2.530243608808817 2314 84-85 2.7837246027841225 2314 86-87 2.9490391464127264 2314 88-89 3.246938404309933 2314 90-91 3.179637749738866 2314 92-93 3.3714942804134758 2314 94-95 3.654469514376121 2314 96-97 3.7964763033302127 2314 98-99 3.867202947405069 2314 100-101 3.642450466772008 2315 1 -0.3563905938274772 2315 2 -0.41514160265708355 2315 3 -0.34959903545802007 2315 4 -0.15721355355766775 2315 5 -0.22902399025675635 2315 6 -0.23447814971717662 2315 7 -0.22435934674364688 2315 8 -0.3190758630092745 2315 9 -0.44175686482908816 2315 10-11 -0.3710284564291513 2315 12-13 -0.2575341164102838 2315 14-15 -0.39750993473416685 2315 16-17 -0.31822911579165947 2315 18-19 -0.19130532632647856 2315 20-21 -0.14327385347688448 2315 22-23 -0.1426447048688999 2315 24-25 -0.25102812044039524 2315 26-27 -0.3327090385360094 2315 28-29 -0.2777631473098694 2315 30-31 -0.30146772811909983 2315 32-33 -0.18627751486201305 2315 34-35 -0.2248280563461691 2315 36-37 -0.12794900236618645 2315 38-39 -0.010360834036291067 2315 40-41 0.015910066385302457 2315 42-43 0.19155647104938112 2315 44-45 0.26085240930876097 2315 46-47 0.1981342932023935 2315 48-49 0.23533739397228004 2315 50-51 0.4289806285639841 2315 52-53 0.5577162898632011 2315 54-55 0.6083469074639609 2315 56-57 0.7163800661998394 2315 58-59 0.8022981808030778 2315 60-61 0.951944107603147 2315 62-63 1.0364845964171785 2315 64-65 1.113357097357433 2315 66-67 1.2575778384118088 2315 68-69 1.515933555334744 2315 70-71 1.349958577701237 2315 72-73 1.6815928978251051 2315 74-75 1.8531464011590266 2315 76-77 1.8022940036261836 2315 78-79 2.241845175493932 2315 80-81 2.1613415405355667 2315 82-83 2.352325258009273 2315 84-85 2.5278088002833776 2315 86-87 2.665098915602698 2315 88-89 2.785618592810888 2315 90-91 3.041897779855123 2315 92-93 3.087556335756677 2315 94-95 3.119327529019696 2315 96-97 3.28641570502867 2315 98-99 3.297800669786813 2315 100-101 3.3329990521003907 2316 1 -0.7539250780217728 2316 2 -0.5921936028479458 2316 3 -0.5440685594110057 2316 4 -0.4207925091003517 2316 5 -0.4646254415728279 2316 6 -0.3471972341680143 2316 7 -0.3410192146544233 2316 8 -0.35194426123798195 2316 9 -0.2990293037642502 2316 10-11 -0.39855932449617626 2316 12-13 -0.41883011677703763 2316 14-15 -0.7923439016304599 2316 16-17 -0.6185165581464744 2316 18-19 -0.5869482438160887 2316 20-21 -0.4010250199469141 2316 22-23 -0.6417355272033731 2316 24-25 -0.8520924454034642 2316 26-27 -0.5817988083108006 2316 28-29 -0.6408813857834019 2316 30-31 -0.445129377428664 2316 32-33 -0.30001091599577734 2316 34-35 -0.2086690431138507 2316 36-37 -0.3011556511752005 2316 38-39 -0.3193192630745898 2316 40-41 -0.3605311217253586 2316 42-43 -0.20176227770508603 2316 44-45 -0.1696153809614671 2316 46-47 -0.1514225323898728 2316 48-49 0.026388625092870654 2316 50-51 0.09760677152684849 2316 52-53 0.1936067740687264 2316 54-55 0.2097406836083735 2316 56-57 0.45418686395775154 2316 58-59 0.5985941998820152 2316 60-61 0.45506529792183414 2316 62-63 0.8617312696268691 2316 64-65 0.7803204299204474 2316 66-67 0.9222964120203514 2316 68-69 1.096007150307905 2316 70-71 0.9294915078496331 2316 72-73 1.1124634676013905 2316 74-75 1.4897762500675888 2316 76-77 1.6425865497259586 2316 78-79 1.5257975932469208 2316 80-81 2.1563379056961196 2316 82-83 2.26203259495842 2316 84-85 2.1013468182713844 2316 86-87 2.4795216497691577 2316 88-89 2.4805743993382556 2316 90-91 2.693078447748686 2316 92-93 2.8716907293840563 2316 94-95 2.8703044213582807 2316 96-97 2.871473754864798 2316 98-99 2.910405493036315 2316 100-101 2.891020469117052 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7128.0 3 2289.0 4 2292.0 5 2670.0 6 3074.0 7 3845.0 8 5072.0 9 6646.0 10 8688.0 11 11342.0 12 14605.0 13 18219.0 14 22152.0 15 26683.0 16 30967.0 17 36453.0 18 34766.0 19 32441.0 20 34235.0 21 37182.0 22 41377.0 23 46659.0 24 52710.0 25 59453.0 26 67194.0 27 77096.0 28 87553.0 29 100087.0 30 115364.0 31 134116.0 32 157416.0 33 185356.0 34 224202.0 35 272794.0 36 322256.0 37 341371.0 38 203981.0 39 18399.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.859291021307676 18.903069902670556 14.86857109049969 23.369067985522076 2 15.260078372807156 19.91755998343613 40.72776355113041 24.094598092626303 3 19.1346537519108 23.760544521625754 29.838607701645536 27.266194024817914 4 12.588702431224661 16.54124272272995 37.38151055296363 33.48854429308176 5 13.530452256363507 37.41468924171923 34.48358601161775 14.571272490299508 6 32.25254899591184 36.39105108601296 17.332434455770972 14.023965462304231 7 28.499642121366865 32.7800672488721 20.507358813542773 18.21293181621826 8 26.437381507474296 34.963575072719905 19.749749405210544 18.84929401459526 9 26.244280477280498 16.800144149358967 20.088217721730622 36.867357651629916 10-11 24.834367541095535 26.632442046200076 27.606006880982047 20.92718353172234 12-13 25.936247899702725 23.9158356794848 27.727295716236494 22.420620704575978 14-15 22.338265631881388 25.502642102106027 26.246016477052024 25.913075788960562 16-17 21.891733243337256 28.610816069989042 27.192728157704316 22.30472252896939 18-19 21.934348039781213 26.942874165759267 29.59783481528659 21.524942979172934 20-21 22.35153767747093 26.22604304892792 29.92238379873316 21.50003547486799 22-23 21.93921013743879 26.29940811672946 29.801660715053995 21.959721030777754 24-25 21.769306701139573 26.390391614090802 29.986018800848708 21.85428288392092 26-27 21.392749274957353 26.805704377197454 29.616707848866564 22.184838498978625 28-29 21.566189481699713 27.296149715115025 29.459480723286806 21.67818007989845 30-31 21.70653069747258 26.71814684019668 29.359820492003223 22.215501970327512 32-33 21.310612334637757 26.71577046354462 29.47372783550632 22.499889366311304 34-35 22.21757059132804 26.99335749449736 29.188302896565094 21.600769017609505 36-37 22.315259664373325 26.433160110778616 29.16516890296616 22.0864113218819 38-39 21.72602941770365 26.84480466918691 29.145316853987822 22.283849059121614 40-41 22.258845244048388 26.633743405046715 29.27072388567062 21.836687465234274 42-43 21.920897090083006 26.684194834469782 29.060611243054257 22.33429683239295 44-45 21.950701370506607 26.970964628706497 29.20332028448445 21.875013716302448 46-47 22.388580157496904 27.254988718867146 28.322561387798817 22.03386973583713 48-49 22.05395227582303 27.35995117530083 28.239508606652507 22.34658794222364 50-51 22.26110604410026 27.492830350331865 28.270918391084088 21.975145214483792 52-53 22.538551642241323 27.128582955465557 28.361852782199986 21.971012620093134 54-55 21.98142175701512 27.4027328381242 28.56569011799695 22.05015528686373 56-57 22.139824538580285 27.606232003669973 28.14719503479049 22.10674842295925 58-59 22.05354829149833 27.13816401644139 28.265454887648378 22.5428328044119 60-61 21.651630409879107 27.36565378002665 28.601946769070167 22.380769041024077 62-63 21.89912372255258 27.313072778622715 28.77152794395111 22.016275554873598 64-65 21.61807944360787 27.45551621104459 28.46039011987523 22.466014225472314 66-67 21.54662446709227 27.527831284097843 28.44807366714255 22.47747058166734 68-69 22.232235342863063 26.973837137851 28.598754182862642 22.195173336423295 70-71 22.000055131118128 27.17274106827603 28.15219629908667 22.67500750151918 72-73 21.87841443105472 27.326878524166908 28.26037069648907 22.534336348289305 74-75 21.939408644891977 27.616464032990713 28.042560094837054 22.40156722728026 76-77 22.08703163501877 27.298430670614643 28.096113853411627 22.51842384095496 78-79 22.147911878161466 27.543797924553875 28.021314158947124 22.286976038337535 80-81 22.34986463852693 27.46536958422433 27.76630745839961 22.41845831884913 82-83 22.275046606135753 27.57301159736934 27.939294213324594 22.212647583170313 84-85 22.20344508732626 27.695616596264117 27.863978793736642 22.23695952267298 86-87 22.464187033054195 27.6517939795207 27.4849807529277 22.399038234497404 88-89 22.33688186903633 27.686532531794683 27.414356583327088 22.5622290158419 90-91 22.262997006337127 27.6869564240128 27.497077982740432 22.552968586909643 92-93 22.459107837042 27.241595995736308 27.75268109424076 22.54661507298093 94-95 22.182320243115665 27.08977787267472 27.943832794241054 22.784069089968565 96-97 22.523861078813287 26.78329936940521 27.862600781211317 22.830238770570183 98-99 22.253857194903016 26.997282954600323 27.50541872786091 23.24344112263575 100-101 22.708803825951808 26.52969448023083 27.89223465973663 22.869267034080725 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 716.0 1 489.0 2 379.0 3 516.5 4 442.5 5 420.5 6 790.5 7 1843.0 8 2930.0 9 3259.0 10 2802.0 11 2250.5 12 2221.0 13 2381.5 14 2537.0 15 2781.5 16 3037.5 17 3376.0 18 3808.5 19 4258.0 20 4970.5 21 6038.5 22 7344.0 23 9140.0 24 11634.0 25 14657.0 26 18690.0 27 23814.5 28 28323.0 29 32702.0 30 37521.5 31 44470.0 32 54639.0 33 59312.5 34 60140.0 35 64225.5 36 69928.0 37 75363.5 38 78727.0 39 81217.5 40 85256.5 41 89928.5 42 92440.5 43 94330.5 44 95883.0 45 99691.5 46 102712.5 47 103612.0 48 103556.0 49 101208.0 50 98615.5 51 95422.0 52 92957.5 53 92534.5 54 93002.0 55 88233.0 56 80820.0 57 73420.0 58 67167.0 59 58877.0 60 46474.5 61 38002.5 62 32466.5 63 24635.5 64 17222.5 65 13069.0 66 9875.0 67 7505.5 68 5835.0 69 4619.0 70 3686.0 71 2781.0 72 2182.0 73 1750.0 74 1298.0 75 970.5 76 754.5 77 535.5 78 349.5 79 237.0 80 155.0 81 92.5 82 68.5 83 47.0 84 29.0 85 18.5 86 13.0 87 12.0 88 10.0 89 6.5 90 5.0 91 4.0 92 4.0 93 2.5 94 1.0 95 1.0 96 2.5 97 2.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.298406008427275 2 0.03412761974247691 3 0.040623102923915425 4 0.044063953474082845 5 0.05716025199665886 6 0.05687936623746153 7 0.02805346519983442 8 0.03184542294899852 9 0.03813024181103902 10-11 0.030370772713212483 12-13 0.032196530147995193 14-15 0.027351250801841066 16-17 0.021119098019650066 18-19 0.0248759450489145 20-21 0.03676092373495198 22-23 0.030388328073162312 24-25 0.05101587601421704 26-27 0.04323885155644066 28-29 0.03577782357776129 30-31 0.03572515749791179 32-33 0.03156453718980118 34-35 0.030335661993312814 36-37 0.029229674316473282 38-39 0.018538460107024497 40-41 0.01759047066973347 42-43 0.018468238667225162 44-45 0.00858457101546873 46-47 0.016080709714047764 48-49 0.013833623640469037 50-51 0.006688592140886679 52-53 0.004739947186455127 54-55 0.0062497081421408335 56-57 0.00549482766429798 58-59 0.006987033260033854 60-61 0.006442817101589006 62-63 0.004195731028010279 64-65 0.007496138698579034 66-67 0.002773746872073741 68-69 0.007127476139632524 70-71 0.013219186042224855 72-73 0.01353518252132186 74-75 0.01056832668979995 76-77 0.009146342533863412 78-79 0.0058634902232444904 80-81 0.007127476139632524 82-83 0.011656759006689645 84-85 0.0037392916693146003 86-87 0.0013166519962375352 88-89 0.018485794027174995 90-91 0.02756191512123907 92-93 0.012938300283027514 94-95 0.02104887657985073 96-97 0.017695802829432474 98-99 0.013008521722826849 100-101 0.10296218610577526 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2848133.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.081514424028875 #Duplication Level Percentage of deduplicated Percentage of total 1 75.1041984802686 41.36852991896042 2 14.15957174790404 15.598613109404965 3 4.785086780922605 7.907094796308553 4 2.1781859752615262 4.799111288583407 5 1.1293074519891861 3.110198235295283 6 0.6678194573387269 2.207070424323014 7 0.42093070523777376 1.6229850498449754 8 0.2931725767748014 1.291871161308075 9 0.19777647262822357 0.9804444866824545 >10 0.9532923199087303 9.446849429296096 >50 0.06813145171213864 2.568284625194579 >100 0.036450099542557644 3.907864276151278 >500 0.002942295826495466 1.144186736637602 >1k 0.0029422958263854234 3.1940411740115895 >5k 1.279259054950184E-4 0.48450720537633063 >10k+ 6.39629527475092E-5 0.3683480826214032 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10455 0.36708257655102483 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7897 0.27726935504767514 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5855 0.20557326501255382 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4312 0.15139742420736674 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4190 0.14711391637960727 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3955 0.1388628972031854 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3721 0.13064698874666317 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3308 0.11614626142810043 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3283 0.11526849343060876 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3077 0.10803568513127722 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2856 0.10027621603345069 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 3.511071989966761E-5 14-15 0.0 0.0 0.0 0.0 3.511071989966761E-5 16-17 0.0 0.0 0.0 0.0 7.022143979933522E-5 18-19 0.0 0.0 0.0 0.0 7.022143979933522E-5 20-21 0.0 0.0 0.0 5.266607984950141E-5 7.022143979933522E-5 22-23 0.0 0.0 0.0 1.9310895944817184E-4 7.022143979933522E-5 24-25 0.0 0.0 0.0 3.862179188963437E-4 7.022143979933522E-5 26-27 0.0 0.0 0.0 7.548804778428536E-4 7.022143979933522E-5 28-29 0.0 0.0 0.0 0.002317307513378062 7.022143979933522E-5 30-31 0.0 0.0 0.0 0.007864801257525544 7.022143979933522E-5 32-33 0.0 0.0 0.0 0.02003066570276037 7.022143979933522E-5 34-35 0.0 0.0 0.0 0.03888512228888187 7.022143979933522E-5 36-37 0.0 0.0 0.0 0.07130987211622491 7.022143979933522E-5 38-39 0.0 0.0 0.0 0.12260663388963927 7.022143979933522E-5 40-41 0.0 0.0 0.0 0.17702824973412407 7.022143979933522E-5 42-43 0.0 0.0 0.0 0.2350487143683248 1.0533215969900282E-4 44-45 0.0 0.0 0.0 0.31067720503220886 1.0533215969900282E-4 46-47 0.0 0.0 0.0 0.4007888676547057 1.0533215969900282E-4 48-49 0.0 0.0 0.0 0.4949382630656644 1.0533215969900282E-4 50-51 0.0 0.0 0.0 0.6008848603629113 1.0533215969900282E-4 52-53 0.0 0.0 0.0 0.7035134946296397 1.0533215969900282E-4 54-55 0.0 0.0 0.0 0.8187117666204493 1.4044287959867044E-4 56-57 0.0 0.0 0.0 0.9570655583850894 1.4044287959867044E-4 58-59 0.0 0.0 0.0 1.1071287752362688 1.4044287959867044E-4 60-61 0.0 0.0 0.0 1.270200513810275 1.4044287959867044E-4 62-63 0.0 0.0 0.0 1.4314113842295988 1.4044287959867044E-4 64-65 0.0 0.0 0.0 1.6044721226150604 1.4044287959867044E-4 66-67 0.0 0.0 0.0 1.8104316055465106 1.4044287959867044E-4 68-69 0.0 0.0 0.0 2.04291723736216 1.4044287959867044E-4 70-71 0.0 0.0 0.0 2.287445846103395 1.4044287959867044E-4 72-73 0.0 0.0 0.0 2.5384523826661187 1.4044287959867044E-4 74-75 0.0 0.0 0.0 2.795919291690381 1.4044287959867044E-4 76-77 0.0 1.7555359949833805E-5 0.0 3.0730482038584572 1.4044287959867044E-4 78-79 0.0 3.511071989966761E-5 0.0 3.389904895593008 1.4044287959867044E-4 80-81 0.0 3.511071989966761E-5 0.0 3.7229125184814054 1.4044287959867044E-4 82-83 0.0 3.511071989966761E-5 0.0 4.06999954004957 1.4044287959867044E-4 84-85 0.0 5.2666079849501414E-5 0.0 4.433588599970577 1.4044287959867044E-4 86-87 3.511071989966761E-5 7.022143979933522E-5 0.0 4.805411123708057 1.4044287959867044E-4 88-89 3.511071989966761E-5 7.022143979933522E-5 0.0 5.207692196958499 1.4044287959867044E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4775 0.0 54.90019 1 GTATCAA 8585 0.0 43.305077 1 ATCAACG 11095 0.0 33.109512 3 TATCAAC 11195 0.0 32.81376 2 TCAACGC 11210 0.0 32.685062 4 CAACGCA 11465 0.0 31.958094 5 AACGCAG 11905 0.0 30.812534 6 ACGCAGA 13630 0.0 26.804577 7 CGCAGAG 13760 0.0 26.378702 8 TGGTATC 2150 0.0 25.640121 2 GTGGTAT 2390 0.0 25.127954 1 TAAGGTG 1880 0.0 24.26688 5 TACATGG 9250 0.0 24.14664 2 GTACATG 9705 0.0 23.917618 1 AGGTAAG 1770 0.0 23.358585 2 ACATGGG 9305 0.0 23.237831 3 GCAGAGT 15540 0.0 22.714785 9 GTAAGGT 2030 0.0 21.771454 4 GTGTAAG 1275 0.0 21.30832 1 GAGTACT 10235 0.0 20.608343 12-13 >>END_MODULE