FastQCFastQC Report
Thu 26 May 2016
SRR938309_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938309_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2848133
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99530.34945699516139167No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83250.29229674316473286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60640.21291140547158435No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA39290.13795001848579402No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC39230.13773935416639602No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA38380.13475494297492427No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA37990.13338562489883724No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA34030.11948177981856888No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG32370.11365340031522404No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50000.057.3607861
GTATCAA88400.041.6981961
ATCAACG117950.030.9562173
TATCAAC119750.030.5832042
TCAACGC119650.030.515854
TGGTATC18400.030.5022582
CAACGCA122150.029.9302145
AACGCAG127200.028.8252016
GTGGTAT22450.027.3300951
TCGCGAT350.008399161527.13964352-53
GTAAGGT18200.025.5995084
ACGCAGA143400.025.3700777
CGCAGAG143850.025.1916648
TAAGGTG20950.024.7354285
AGGTAAG19400.022.5555762
GCAGAGT164400.021.6093649
GAGTACT108850.021.05285812-13
GGTAAGG21400.020.883283
AGAGTAC150300.020.77681410-11
GTACATG93850.020.5759351
Produced by FastQC (version 0.11.5)