##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938309_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2848133 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.662660065383182 31.0 31.0 34.0 30.0 34.0 2 31.917504905845338 31.0 31.0 34.0 30.0 34.0 3 31.978214851623854 31.0 31.0 34.0 30.0 34.0 4 35.678217976477924 37.0 35.0 37.0 33.0 37.0 5 35.548382045360945 37.0 35.0 37.0 33.0 37.0 6 35.58967541192774 37.0 35.0 37.0 33.0 37.0 7 35.521592566077494 37.0 35.0 37.0 33.0 37.0 8 35.50065358605093 37.0 35.0 37.0 33.0 37.0 9 37.046673382177026 39.0 37.0 39.0 33.0 39.0 10-11 36.972262180172066 39.0 37.0 39.0 33.0 39.0 12-13 36.85417991364869 39.0 37.0 39.0 32.5 39.0 14-15 37.84672959443959 40.0 37.0 41.0 32.5 41.0 16-17 37.75751448404973 39.0 37.0 41.0 32.0 41.0 18-19 37.76493566136132 39.5 37.0 41.0 32.0 41.0 20-21 37.75619800760709 39.5 37.0 41.0 32.0 41.0 22-23 37.78584427061517 40.0 37.0 41.0 32.0 41.0 24-25 37.69824460444789 39.5 37.0 41.0 32.0 41.0 26-27 37.49908220578182 39.0 36.5 41.0 32.0 41.0 28-29 37.35294454296903 39.0 36.0 41.0 31.5 41.0 30-31 37.06731269220924 39.0 36.0 40.5 31.0 41.0 32-33 36.81511221561634 39.0 35.5 40.0 30.5 41.0 34-35 36.61208079117092 39.0 35.0 40.0 30.0 41.0 36-37 36.58491668050614 39.0 35.0 40.0 30.0 41.0 38-39 36.403900379652214 39.0 35.0 40.0 30.0 41.0 40-41 36.19972750570285 38.0 35.0 40.0 30.0 41.0 42-43 35.936979417744894 38.0 35.0 40.0 29.0 41.0 44-45 35.62300320947091 38.0 34.5 40.0 28.0 41.0 46-47 35.22080183755463 38.0 34.0 40.0 26.5 41.0 48-49 34.772598751533025 38.0 33.5 40.0 25.5 41.0 50-51 34.35381089998255 38.0 33.0 40.0 24.0 41.0 52-53 33.79175164923829 37.0 33.0 40.0 23.0 41.0 54-55 33.42015558964417 37.0 32.0 40.0 22.5 41.0 56-57 33.01686438098221 36.5 31.5 39.0 20.5 40.5 58-59 32.51643936571783 36.0 31.0 39.0 18.5 40.0 60-61 32.07010522331647 35.5 31.0 39.0 17.0 40.0 62-63 31.539683715613002 35.0 30.0 38.5 13.0 40.0 64-65 30.999144878416843 35.0 29.0 38.0 8.5 40.0 66-67 30.463996063386084 34.0 28.5 38.0 7.5 40.0 68-69 29.842980471768698 34.0 28.0 37.0 6.5 39.5 70-71 29.226564033350968 34.0 27.0 36.5 2.0 39.0 72-73 28.594507524753936 33.0 26.0 36.0 2.0 39.0 74-75 27.93651718511741 33.0 26.0 35.5 2.0 38.0 76-77 26.72875055343272 31.0 24.5 34.0 2.0 36.0 78-79 27.31429852468266 32.0 26.0 35.0 2.0 37.0 80-81 26.961543404047497 32.0 25.0 35.0 2.0 36.5 82-83 26.399969032345048 32.0 24.0 35.0 2.0 36.0 84-85 25.733906211542788 31.5 21.5 34.5 2.0 36.0 86-87 25.013753922306297 31.0 19.0 34.0 2.0 35.0 88-89 24.164374346282287 31.0 14.5 34.0 2.0 35.0 90-91 23.53034601965568 30.0 7.0 34.0 2.0 35.0 92-93 22.676881135817744 30.0 2.0 34.0 2.0 35.0 94-95 21.79996457328362 29.0 2.0 34.0 2.0 35.0 96-97 20.73969825145104 29.0 2.0 34.0 2.0 35.0 98-99 19.49223842425898 27.5 2.0 34.0 2.0 35.0 100-101 16.547349614642293 20.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27839769871316733 1101 2 0.35272272123646076 1101 3 0.30542682306336033 1101 4 0.2524188993041818 1101 5 0.24584630120779138 1101 6 0.24022864394206067 1101 7 0.19294208722048012 1101 8 0.30528623045942993 1101 9 0.3146210650613739 1101 10-11 0.3230299057200128 1101 12-13 0.3114544343714982 1101 14-15 0.46054860934040676 1101 16-17 0.4891557286197923 1101 18-19 0.508026061291666 1101 20-21 0.6217204748356195 1101 22-23 0.6119501513464058 1101 24-25 0.5595343446896379 1101 26-27 0.584991484701149 1101 28-29 0.5135454857245847 1101 30-31 0.711416507094917 1101 32-33 0.6406711357289581 1101 34-35 0.4980233992088543 1101 36-37 0.6792386573642304 1101 38-39 0.8071482165281765 1101 40-41 0.9444965956874398 1101 42-43 0.7851942851332652 1101 44-45 0.7775354159324337 1101 46-47 0.9606728821860671 1101 48-49 1.2378847325670819 1101 50-51 1.2200023038695988 1101 52-53 1.169295333783225 1101 54-55 1.1983737596275645 1101 56-57 1.255862387929902 1101 58-59 1.4035477671784022 1101 60-61 1.4186771860564136 1101 62-63 1.4844402578339526 1101 64-65 1.5755556266142712 1101 66-67 1.5678228931373468 1101 68-69 1.610802955503889 1101 70-71 1.7037987889991832 1101 72-73 1.8585380655923416 1101 74-75 1.9991154090408898 1101 76-77 1.759381597869389 1101 78-79 2.1037387719974276 1101 80-81 2.5402249961509753 1101 82-83 2.7585481628970676 1101 84-85 2.8123559830893576 1101 86-87 2.860972857362814 1101 88-89 3.098490145925247 1101 90-91 3.456459586742259 1101 92-93 3.8344316332837103 1101 94-95 3.8823010174518693 1101 96-97 4.23135882328015 1101 98-99 4.587012205229737 1101 100-101 4.719784181701254 1102 1 0.31962191986279365 1102 2 0.3764747042249468 1102 3 0.3236804430721385 1102 4 0.3220800192285722 1102 5 0.18256280979792905 1102 6 0.23160306592200897 1102 7 0.2806222438343795 1102 8 0.2951856465326017 1102 9 0.4572713502741905 1102 10-11 0.49958085586684575 1102 12-13 0.4431577792933652 1102 14-15 0.597448227931551 1102 16-17 0.5394586617551127 1102 18-19 0.551773318860775 1102 20-21 0.6499902962822688 1102 22-23 0.561943400747289 1102 24-25 0.5297236989948289 1102 26-27 0.539989459521415 1102 28-29 0.5538355927215832 1102 30-31 0.47620791358223613 1102 32-33 0.37951914598986747 1102 34-35 0.5107440594174477 1102 36-37 0.657286552864953 1102 38-39 0.6805407301137478 1102 40-41 0.6970896695727404 1102 42-43 0.7376017175428871 1102 44-45 0.7151016561862562 1102 46-47 0.8028497816358922 1102 48-49 0.862842541322614 1102 50-51 0.9650397696946911 1102 52-53 0.968398347925735 1102 54-55 0.9733104727608506 1102 56-57 1.020407397549505 1102 58-59 1.1828478988150906 1102 60-61 1.1586449519456323 1102 62-63 1.397445287030294 1102 64-65 1.4276778462112638 1102 66-67 1.5333020169095875 1102 68-69 1.596300137128761 1102 70-71 1.7705293050824835 1102 72-73 1.7787307951970952 1102 74-75 1.9548171338189633 1102 76-77 1.8613148006911366 1102 78-79 2.2399548924281163 1102 80-81 2.5681842900383103 1102 82-83 2.4457076795541717 1102 84-85 2.714057134066504 1102 86-87 2.9503339131565163 1102 88-89 2.9763102087058186 1102 90-91 3.1848812966690154 1102 92-93 3.4062681337731284 1102 94-95 3.7319617998463066 1102 96-97 3.9472801112944644 1102 98-99 4.323320302573375 1102 100-101 4.6739079539360375 1103 1 0.3378343240577024 1103 2 0.300419822473458 1103 3 0.2906200961223071 1103 4 0.1985747427683151 1103 5 0.17571407926667604 1103 6 0.18510620882135242 1103 7 0.2713232006543862 1103 8 0.17527131720927258 1103 9 0.3179432578417618 1103 10-11 0.40627613413241903 1103 12-13 0.4337980116873794 1103 14-15 0.5765573841902736 1103 16-17 0.5823785219248023 1103 18-19 0.4963983865603083 1103 20-21 0.5282230513658916 1103 22-23 0.4416827780757444 1103 24-25 0.6341139108738361 1103 26-27 0.5456261633339281 1103 28-29 0.5212101639578961 1103 30-31 0.5339937697145842 1103 32-33 0.5660982272457105 1103 34-35 0.6903693732970808 1103 36-37 0.861238564156416 1103 38-39 0.8737551858095429 1103 40-41 0.9425542114315704 1103 42-43 1.0004227774302947 1103 44-45 1.0093615567294947 1103 46-47 1.0054043105292294 1103 48-49 1.1583202127870535 1103 50-51 1.143700057561297 1103 52-53 1.2303348006345303 1103 54-55 1.2831995275933679 1103 56-57 1.2905506743708273 1103 58-59 1.4900995441187845 1103 60-61 1.5910200976021969 1103 62-63 1.669937122056794 1103 64-65 1.5769187908863849 1103 66-67 1.5546006990254213 1103 68-69 1.6614131337971223 1103 70-71 1.788669496446488 1103 72-73 1.740463739018793 1103 74-75 1.9879554378021567 1103 76-77 2.0165469485304968 1103 78-79 2.099309736431991 1103 80-81 2.450525791080473 1103 82-83 2.5518515143837313 1103 84-85 2.7192047600696867 1103 86-87 2.965252427541639 1103 88-89 3.2285233678530503 1103 90-91 3.4093097485243895 1103 92-93 3.6802559232399013 1103 94-95 3.686677790064749 1103 96-97 4.139439274938585 1103 98-99 4.366991832664791 1103 100-101 4.567191340061324 1104 1 0.3209610248194714 1104 2 0.31378535350803816 1104 3 0.2714587852518555 1104 4 0.198951318408227 1104 5 0.17188322078069973 1104 6 0.19128243654022725 1104 7 0.18279089695049322 1104 8 0.20887754687394278 1104 9 0.2612480527435679 1104 10-11 0.27027134029548705 1104 12-13 0.35407723913616707 1104 14-15 0.503697016307818 1104 16-17 0.5890948065462922 1104 18-19 0.5409458810738172 1104 20-21 0.524333504195674 1104 22-23 0.4179715815816749 1104 24-25 0.4749011911361407 1104 26-27 0.5332398257691935 1104 28-29 0.5102940686890989 1104 30-31 0.5712589060707884 1104 32-33 0.5820408570602424 1104 34-35 0.6178325797273274 1104 36-37 0.7144116979530608 1104 38-39 0.8557494974844531 1104 40-41 0.8999072622266837 1104 42-43 0.9101908010277455 1104 44-45 0.8742091404014047 1104 46-47 0.9738276202709173 1104 48-49 1.1322441648370685 1104 50-51 1.075008764396678 1104 52-53 1.2067181110732292 1104 54-55 1.156458228281565 1104 56-57 1.4106716480850707 1104 58-59 1.546824638093291 1104 60-61 1.5755049876256493 1104 62-63 1.7720519234155745 1104 64-65 1.7883080500585216 1104 66-67 1.9050671349064032 1104 68-69 2.0950309075864872 1104 70-71 2.122113188707715 1104 72-73 2.3417182973749533 1104 74-75 2.5628909649548106 1104 76-77 2.318248049118786 1104 78-79 2.558360039773831 1104 80-81 2.8692883093163175 1104 82-83 3.0108760431075474 1104 84-85 3.272366664361389 1104 86-87 3.385182911097676 1104 88-89 3.3455587688673845 1104 90-91 3.7336542745763595 1104 92-93 4.151306549914043 1104 94-95 4.256244089158741 1104 96-97 4.537481541439906 1104 98-99 4.773969058067092 1104 100-101 4.773643973331833 1105 1 0.33841723423255843 1105 2 0.35263804106165964 1105 3 0.3756232416827636 1105 4 0.24641694575223738 1105 5 0.18173110072255838 1105 6 0.1849123044100054 1105 7 0.16494987385794957 1105 8 0.31758159490911453 1105 9 0.4003188789343497 1105 10-11 0.40162123131923266 1105 12-13 0.4690343931342227 1105 14-15 0.6047429464029506 1105 16-17 0.5353588566660576 1105 18-19 0.43617837926375813 1105 20-21 0.5398763474057375 1105 22-23 0.3858070674519851 1105 24-25 0.4225437304038877 1105 26-27 0.6636246580611598 1105 28-29 0.646558593427919 1105 30-31 0.7462742148616641 1105 32-33 0.7950947886741488 1105 34-35 0.7042772270481521 1105 36-37 0.7636950697212299 1105 38-39 0.8029981494732894 1105 40-41 0.7456746840029496 1105 42-43 0.8618862147187443 1105 44-45 0.7879427078801342 1105 46-47 1.0059344356143285 1105 48-49 0.9993645580077626 1105 50-51 1.1559380591872142 1105 52-53 1.2128332517931213 1105 54-55 1.1731462062741258 1105 56-57 1.2246237729824756 1105 58-59 1.4422365143647582 1105 60-61 1.6259607149746955 1105 62-63 1.6638536703859188 1105 64-65 1.7625173905613956 1105 66-67 1.8596088669495145 1105 68-69 1.880379091842908 1105 70-71 1.9495130859666503 1105 72-73 2.0997335428832855 1105 74-75 2.1420134501718238 1105 76-77 2.1962830518082974 1105 78-79 2.558593161482907 1105 80-81 2.6051023182093758 1105 82-83 2.648828257345002 1105 84-85 2.8905002761378995 1105 86-87 3.215421914360448 1105 88-89 3.3102752630891317 1105 90-91 3.56183938717275 1105 92-93 3.744203922385765 1105 94-95 3.974601423325918 1105 96-97 4.290003536775611 1105 98-99 4.588063568679015 1105 100-101 4.785478273585149 1106 1 0.28015169697310327 1106 2 0.2894369310114655 1106 3 0.1829814189967145 1106 4 0.1173326627154978 1106 5 0.12592986001252626 1106 6 0.1264676217254106 1106 7 0.21536977212196007 1106 8 0.1372040214967143 1106 9 0.25610401982175546 1106 10-11 0.3484606407070103 1106 12-13 0.14814445449967195 1106 14-15 0.22626217405816362 1106 16-17 0.2804528312376462 1106 18-19 0.39468387833033347 1106 20-21 0.5675433807388899 1106 22-23 0.4358042290439528 1106 24-25 0.24479145176714212 1106 26-27 0.37394627295712723 1106 28-29 0.49349022781118634 1106 30-31 0.6050380555906756 1106 32-33 0.5546060148105809 1106 34-35 0.47259938356820896 1106 36-37 0.5798095031656842 1106 38-39 0.5741994288973444 1106 40-41 0.48529820872874296 1106 42-43 0.6997998545256863 1106 44-45 0.2910472748321311 1106 46-47 0.3083612279526804 1106 48-49 0.7973676216271954 1106 50-51 0.5103654173459162 1106 52-53 0.680312075407933 1106 54-55 0.6629740033158598 1106 56-57 0.5188678072071582 1106 58-59 1.1467903445404772 1106 60-61 0.9215269354994575 1106 62-63 1.2022645434430075 1106 64-65 1.0223615480009052 1106 66-67 1.1149436781162763 1106 68-69 0.9513409213039097 1106 70-71 1.100679718200169 1106 72-73 1.4442560202830421 1106 74-75 1.8334670966420532 1106 76-77 1.9242151273080879 1106 78-79 2.158668681749141 1106 80-81 2.0536161556811976 1106 82-83 2.470752586317424 1106 84-85 2.4721266094917063 1106 86-87 2.871465489972845 1106 88-89 3.2209017043955086 1106 90-91 3.116027388430954 1106 92-93 2.871128223439417 1106 94-95 3.2920808518473557 1106 96-97 4.010904038010555 1106 98-99 4.169030786462152 1106 100-101 4.228205015855838 1107 1 0.10611530514283274 1107 2 0.1616168623701384 1107 3 0.14902402433078166 1107 4 0.11882368675080102 1107 5 0.04832205897477593 1107 6 0.027605950802545465 1107 7 0.0936168158279429 1107 8 0.21133909200860046 1107 9 0.17377373902127147 1107 10-11 0.16168209301863357 1107 12-13 0.19440211971868848 1107 14-15 0.3427328388380033 1107 16-17 0.3654696662544836 1107 18-19 0.3156643486264272 1107 20-21 0.25673670962626005 1107 22-23 0.2528847226542368 1107 24-25 0.3642089222313132 1107 26-27 0.3470258339996519 1107 28-29 0.3145280347588084 1107 30-31 0.2946759807358461 1107 32-33 0.44499744928413776 1107 34-35 0.52413052970482 1107 36-37 0.621181353716274 1107 38-39 0.6299454288865007 1107 40-41 0.6246960790042309 1107 42-43 0.7473919862563108 1107 44-45 0.8159744857496776 1107 46-47 0.8944685437298787 1107 48-49 0.9344150958324775 1107 50-51 1.038710461995315 1107 52-53 1.0848811152426734 1107 54-55 1.0095496632973138 1107 56-57 0.8865414572882173 1107 58-59 1.0222539174678147 1107 60-61 1.1907184480484574 1107 62-63 1.4862707352591826 1107 64-65 1.5405344200385152 1107 66-67 1.637761090023865 1107 68-69 1.8351162754898915 1107 70-71 1.8184342902220507 1107 72-73 1.8996838899621196 1107 74-75 2.0296479063494353 1107 76-77 2.0613893385563706 1107 78-79 2.4041529121367518 1107 80-81 2.584184181619598 1107 82-83 2.837642093303547 1107 84-85 3.0827971907742047 1107 86-87 3.3381777006464084 1107 88-89 3.565936187715014 1107 90-91 3.702391566985149 1107 92-93 3.7582665145632177 1107 94-95 3.9388660684795944 1107 96-97 4.233259096919557 1107 98-99 4.304211091932313 1107 100-101 4.362437843325381 1108 1 0.12086967936648207 1108 2 0.12251314628715448 1108 3 0.1288317096393108 1108 4 0.14460166548973774 1108 5 0.06282894267949501 1108 6 0.027593747550319847 1108 7 0.09417120743587049 1108 8 0.12262251875033314 1108 9 0.06839490939451309 1108 10-11 0.12657756467005044 1108 12-13 0.2368403209507548 1108 14-15 0.2957248407836772 1108 16-17 0.22480850475322 1108 18-19 0.2601265984008805 1108 20-21 0.2707796021310216 1108 22-23 0.28413912492395355 1108 24-25 0.2698585677161347 1108 26-27 0.328122139925334 1108 28-29 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3.4104578607005998 1108 96-97 3.6666354413647007 1108 98-99 4.053625068210049 1108 100-101 4.074625959241718 1109 1 0.2247305898254588 1109 2 0.20554443964194746 1109 3 0.11086255629026809 1109 4 0.08541718914031549 1109 5 0.11326703009823547 1109 6 0.08646498432650418 1109 7 0.13726666603221815 1109 8 0.17616717010898952 1109 9 0.15043935846230738 1109 10-11 0.2068651027733992 1109 12-13 0.288338966352498 1109 14-15 0.3065002471951459 1109 16-17 0.37389576396629565 1109 18-19 0.38766217209531817 1109 20-21 0.4322405808231622 1109 22-23 0.3530937786086312 1109 24-25 0.35891954831657813 1109 26-27 0.3559767828002194 1109 28-29 0.3697299158024734 1109 30-31 0.4252101790209437 1109 32-33 0.4295982794227271 1109 34-35 0.5326567506779298 1109 36-37 0.5561270607280235 1109 38-39 0.5219837191349939 1109 40-41 0.58995100768675 1109 42-43 0.6149262581302324 1109 44-45 0.6415857683321917 1109 46-47 0.5987766035462769 1109 48-49 0.5374891629879457 1109 50-51 0.5805607469397458 1109 52-53 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90-91 2.43337000097004 2316 92-93 2.3253012705814022 2316 94-95 2.81578245516107 2316 96-97 2.95710137085171 2316 98-99 3.177471398295662 2316 100-101 3.407523768541406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 22.0 4 49.0 5 124.0 6 237.0 7 644.0 8 1500.0 9 2758.0 10 4473.0 11 7150.0 12 10205.0 13 14079.0 14 18442.0 15 24278.0 16 29848.0 17 37000.0 18 35475.0 19 32531.0 20 32738.0 21 34832.0 22 38116.0 23 42901.0 24 49049.0 25 55704.0 26 65362.0 27 76874.0 28 90928.0 29 106443.0 30 122259.0 31 142930.0 32 167706.0 33 197574.0 34 238568.0 35 289870.0 36 343118.0 37 352753.0 38 173284.0 39 8301.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.26534207871759 20.110240715753594 15.716692806615987 22.907724398912833 2 15.882971974841778 21.04510734676014 39.269222906543234 23.802697771854852 3 19.266907690562956 24.17297402819467 30.502186173580043 26.057932107662335 4 13.273888543828535 17.620700999567084 36.839115308168545 32.26629514843584 5 13.396507841225635 37.66582341831187 34.64815878279829 14.289509957664201 6 32.12293807908549 37.1077474261209 16.921611455644804 13.847703039148804 7 28.290357226997475 32.929536647340555 20.517089616250363 18.263016509411603 8 26.35178904917713 35.62035199901128 19.447792641705988 18.580066310105604 9 25.98993796989115 16.728256721157333 19.96500865654799 37.316796652403525 10-11 24.742366315056213 26.959274724881176 27.495573415988652 20.80278554407396 12-13 25.88885420730001 24.063026551077495 27.78074268301375 22.267376558608746 14-15 22.14343571736292 25.716074354673747 26.21020507118172 25.930284856781615 16-17 21.717419797460302 28.814016058941068 27.26221001617551 22.206354127423122 18-19 21.792697180925188 27.085269543241136 29.739692633735853 21.382340642097823 20-21 22.14854432710832 26.330125734999033 30.07712420733161 21.444205730561038 22-23 21.84768056828807 26.354685683568853 30.009009410726257 21.788624337416827 24-25 21.787364760622705 26.545979106056038 30.022457092417465 21.64419904090379 26-27 21.37873603660926 26.91177131572257 29.768130110077802 21.94136253759037 28-29 21.56837479148621 27.27648252381472 29.642119943134677 21.513022741564384 30-31 21.699794216070668 26.78159692682891 29.56164617312464 21.956962683975785 32-33 21.28059913205904 26.791199106779207 29.627754448548515 22.300447312613233 34-35 22.136621442696786 27.004681471867535 29.331353500727165 21.527343584708515 36-37 22.26683780229411 26.511205012160143 29.364507123950613 21.85745006159513 38-39 21.66392449479046 26.855767962923004 29.34818009739787 22.132127444888667 40-41 22.243494904475707 26.690901723543732 29.409638298272036 21.65596507370853 42-43 21.90398106728278 26.787477440150564 29.157982148751042 22.15055934381562 44-45 21.94361868553382 26.973053701413967 29.318638150582494 21.76468946246972 46-47 22.37526602967595 27.262136597274388 28.476388818423587 21.886208554626077 48-49 22.03668157350798 27.391715906525434 28.37437717971738 22.197225340249208 50-51 22.29523691484913 27.481107799389985 28.34135554765174 21.88229973810914 52-53 22.670675842736276 27.132651459745734 28.33405251791261 21.862620179605376 54-55 22.0452134784436 27.422560673957292 28.525879936084447 22.006345911514664 56-57 22.224804810730397 27.5904952472374 28.151704994113686 22.032994947918514 58-59 22.07860377306818 27.249341937332282 28.215378284651738 22.456676004947802 60-61 21.740505095794333 27.45003481227878 28.51659315067098 22.292866941255905 62-63 21.896747799347853 27.44712062252711 28.598927788835702 22.057203789289336 64-65 21.618390011983287 27.523661991908384 28.3987264639678 22.45922153214053 66-67 21.548313092242598 27.633754200789255 28.339990635946343 22.4779420710218 68-69 22.2179679613781 27.150195303928022 28.454386657888964 22.177450076804917 70-71 21.96900566090137 27.256399192032116 28.06471818556226 22.709876961504254 72-73 21.89922092768907 27.396615534889374 28.079870623928464 22.624292913493093 74-75 21.9391791043466 27.682064004735736 27.786851948276293 22.59190494264137 76-77 21.92794367397871 27.316192748021244 28.19231054167765 22.56355303632239 78-79 21.92222062663506 27.534827200836475 28.17515895500667 22.367793217521793 80-81 22.14503513796155 27.45822215660413 27.936693912024047 22.460048793410273 82-83 22.07747862918509 27.607636174483247 28.0110979190875 22.303787277244158 84-85 21.97346914770164 27.683448589451213 27.92163286230186 22.421449400545292 86-87 22.164411467912135 27.62664118990789 27.636525020663 22.572422321516978 88-89 21.935092556262745 27.773584971907027 27.47321271043394 22.818109761396283 90-91 21.876699597380853 27.70282725226388 27.65077502518345 22.769698125171825 92-93 21.93644771872046 27.32073967896451 27.880247636385658 22.86256496592937 94-95 21.676954692775936 27.139340051886624 27.9971651604753 23.186540094862142 96-97 21.900978641095765 26.770624826860264 28.02146177864587 23.306934753398103 98-99 21.57704713930143 27.12784129111948 27.657468945446016 23.637642624133072 100-101 21.7047254980272 26.78038525514705 28.074084152223623 23.440805094602126 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 716.0 1 505.0 2 374.0 3 494.0 4 444.5 5 442.5 6 843.0 7 1855.0 8 2881.0 9 3174.5 10 2658.0 11 2117.5 12 2119.0 13 2270.0 14 2496.5 15 2797.5 16 3005.5 17 3309.5 18 3815.5 19 4371.0 20 5016.0 21 5925.5 22 7349.5 23 9237.5 24 11594.0 25 14779.0 26 18838.5 27 23943.0 28 28923.0 29 33529.0 30 38382.0 31 45402.5 32 55837.5 33 60270.0 34 60961.0 35 65225.0 36 70439.0 37 75463.0 38 79319.0 39 82444.5 40 86178.0 41 90445.5 42 93045.0 43 94855.0 44 96930.0 45 100881.0 46 103143.0 47 104392.0 48 104525.0 49 101582.5 50 99275.0 51 96303.0 52 93195.5 53 92823.5 54 93832.5 55 88115.0 56 79467.0 57 72002.5 58 65202.5 59 56566.0 60 44584.5 61 36913.5 62 31594.0 63 23383.5 64 15848.5 65 11612.0 66 8788.0 67 6888.0 68 5373.5 69 4204.0 70 3345.5 71 2472.0 72 1872.5 73 1496.5 74 1147.0 75 871.0 76 614.0 77 416.0 78 281.0 79 199.5 80 117.5 81 57.0 82 36.0 83 23.5 84 10.0 85 5.0 86 4.5 87 2.5 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.02214397993352E-5 2 0.04181686740050412 3 2.1066431939800563E-4 4 0.0 5 0.09785357636037362 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0012288751964883663 26-27 0.011551426846990643 28-29 0.0 30-31 0.0 32-33 4.564393586956789E-4 34-35 0.014658725558111227 36-37 0.003493516630016927 38-39 0.011568982206940475 40-41 0.006758813580686015 42-43 0.005371940144649144 44-45 0.0027913022320235747 46-47 5.266607984950141E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.6333039924750706E-4 68-69 2.808857591973408E-4 70-71 0.0 72-73 7.02214397993352E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.777679974916902E-5 82-83 0.0011059876768395296 84-85 0.004494172147157453 86-87 0.0016502038352843775 88-89 1.75553599498338E-5 90-91 0.0011235430367893633 92-93 1.9310895944817184E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.19769757943186E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2848133.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.88496762512281 #Duplication Level Percentage of deduplicated Percentage of total 1 74.0605767816455 39.9075178217689 2 14.550482415176322 15.6810454774339 3 5.14905586792699 8.323701262295838 4 2.274139310573311 4.901676925010479 5 1.205063367495759 3.2467400271865223 6 0.7080135033068989 2.289077082230522 7 0.43287549523659324 1.6327837432572982 8 0.29003489510130875 1.2502816746151932 9 0.20464304855881618 0.9924465641668422 >10 1.0080195738408775 9.737568168924186 >50 0.07088706337480358 2.630870138325857 >100 0.03974678989103696 4.162103372033022 >500 0.003133036543940779 1.1697715011789518 >1k 0.0031330365438158162 3.2182696983560417 >5k 1.9581478398848851E-4 0.8561465432163535 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9953 0.34945699516139167 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8325 0.29229674316473286 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6064 0.21291140547158435 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3929 0.13795001848579402 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3923 0.13773935416639602 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3838 0.13475494297492427 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3799 0.13338562489883724 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3403 0.11948177981856888 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3237 0.11365340031522404 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.266607984950141E-5 0.0 22-23 0.0 0.0 0.0 1.9310895944817184E-4 0.0 24-25 0.0 0.0 0.0 3.862179188963437E-4 0.0 26-27 1.7555359949833805E-5 0.0 0.0 7.724358377926874E-4 0.0 28-29 3.511071989966761E-5 0.0 0.0 0.002299752153428228 0.0 30-31 3.511071989966761E-5 0.0 0.0 0.007812135177676043 0.0 32-33 3.511071989966761E-5 0.0 0.0 0.019820001383362363 0.0 34-35 3.511071989966761E-5 0.0 0.0 0.038709568689383536 0.0 36-37 3.511071989966761E-5 0.0 0.0 0.07076565595778006 0.0 38-39 3.511071989966761E-5 0.0 0.0 0.12218530525084327 0.0 40-41 3.511071989966761E-5 0.0 0.0 0.17671225325502704 0.0 42-43 3.511071989966761E-5 0.0 0.0 0.23466249644942846 0.0 44-45 3.511071989966761E-5 0.0 0.0 0.30988721383446627 0.0 46-47 3.511071989966761E-5 0.0 0.0 0.3998584335773645 0.0 48-49 3.511071989966761E-5 0.0 0.0 0.4937620539490256 0.0 50-51 3.511071989966761E-5 0.0 0.0 0.598707995729132 0.0 52-53 3.511071989966761E-5 0.0 0.0 0.7011259656764625 0.0 54-55 3.511071989966761E-5 0.0 0.0 0.8157273554289775 0.0 56-57 3.511071989966761E-5 0.0 0.0 0.9530102702366778 0.0 58-59 3.511071989966761E-5 0.0 0.0 1.1022308298102652 0.0 60-61 3.511071989966761E-5 0.0 0.0 1.2637752520686358 0.0 62-63 3.511071989966761E-5 0.0 0.0 1.4222650416957354 3.511071989966761E-5 64-65 3.511071989966761E-5 0.0 0.0 1.593429801206615 3.511071989966761E-5 66-67 3.511071989966761E-5 0.0 0.0 1.796878867665239 3.511071989966761E-5 68-69 3.511071989966761E-5 0.0 0.0 2.0279425153249515 3.511071989966761E-5 70-71 3.511071989966761E-5 0.0 0.0 2.2703644808722068 3.511071989966761E-5 72-73 3.511071989966761E-5 0.0 0.0 2.519387261760599 3.511071989966761E-5 74-75 3.511071989966761E-5 0.0 0.0 2.7759764027873697 3.511071989966761E-5 76-77 3.511071989966761E-5 0.0 0.0 3.053631975753941 3.511071989966761E-5 78-79 3.511071989966761E-5 0.0 0.0 3.370822219327539 3.511071989966761E-5 80-81 3.511071989966761E-5 0.0 0.0 3.704532056613929 3.511071989966761E-5 82-83 3.511071989966761E-5 0.0 0.0 4.0508466423443 3.511071989966761E-5 84-85 3.511071989966761E-5 0.0 0.0 4.414821920184204 3.511071989966761E-5 86-87 3.511071989966761E-5 0.0 0.0 4.787820653038324 3.511071989966761E-5 88-89 3.511071989966761E-5 0.0 0.0 5.189838395889518 3.511071989966761E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5000 0.0 57.360786 1 GTATCAA 8840 0.0 41.698196 1 ATCAACG 11795 0.0 30.956217 3 TATCAAC 11975 0.0 30.583204 2 TCAACGC 11965 0.0 30.51585 4 TGGTATC 1840 0.0 30.502258 2 CAACGCA 12215 0.0 29.930214 5 AACGCAG 12720 0.0 28.825201 6 GTGGTAT 2245 0.0 27.330095 1 TCGCGAT 35 0.0083991615 27.139643 52-53 GTAAGGT 1820 0.0 25.599508 4 ACGCAGA 14340 0.0 25.370077 7 CGCAGAG 14385 0.0 25.191664 8 TAAGGTG 2095 0.0 24.735428 5 AGGTAAG 1940 0.0 22.555576 2 GCAGAGT 16440 0.0 21.609364 9 GAGTACT 10885 0.0 21.052858 12-13 GGTAAGG 2140 0.0 20.88328 3 AGAGTAC 15030 0.0 20.776814 10-11 GTACATG 9385 0.0 20.575935 1 >>END_MODULE