FastQCFastQC Report
Thu 26 May 2016
SRR938308_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938308_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2807240
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96280.34297031960217156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74610.2657770621678232No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52620.1874438950713156No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA42320.15075305282056398No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA39800.14177626423105968No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA39200.13963893361451105No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA35630.12692181644604664No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC32500.11577207506305125No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA32270.11495276499337427No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG29650.10561975463444521No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45950.055.249551
GTATCAA87100.045.3095741
ATCAACG112000.035.012553
TATCAAC113650.034.4205862
TCAACGC114250.034.323034
CAACGCA117050.033.460775
AACGCAG121600.032.2380566
ACGCAGA137000.028.259827
CGCAGAG137150.028.0210938
GCAGAGT153000.024.5886769
TGGTATC20350.023.8244882
TACATGG87800.023.711962
GTACATG91650.023.4305611
GTGGTAT22300.023.111151
TAAGGTG18450.022.9284025
ACATGGG87250.022.826353
AGGTAAG16350.022.6759092
GTAAGGT18600.021.72174
GAGTACT102250.021.6943812-13
AGAGTAC140500.021.6706310-11
Produced by FastQC (version 0.11.5)