FastQCFastQC Report
Thu 26 May 2016
SRR938308_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938308_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2807240
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94660.3371995269374902No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79610.28358815063906184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59810.21305624029295678No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC38890.13853464612929425No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA38650.1376797138826748No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37050.13198016557187842No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA36860.13130334420997136No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA33940.12090166854276799No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG31810.11331414485402033No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49100.056.7078321
GTATCAA88600.041.186461
ATCAACG118650.030.4646953
TATCAAC119550.030.4367162
TCAACGC119900.030.1470894
TAAGGTG19450.029.7934065
CAACGCA122150.029.5528955
GTAAGGT19300.028.794434
GTGGTAT22750.028.6131761
TGGTATC21850.028.2624972
AACGCAG128150.028.2062916
GGTAAGG20900.025.6810113
AGGTAAG20200.025.3975032
ACGCAGA143600.025.1715627
CGCAGAG144800.024.9301538
AAGGTAA20800.022.8435521
TACATGG89750.022.7060412
GTACATG93600.022.3866791
GGTGTGC26300.022.0335278
ACATGGG89650.021.9875953
Produced by FastQC (version 0.11.5)