FastQCFastQC Report
Thu 26 May 2016
SRR938307_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938307_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1406386
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59330.4218614235352173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42870.30482385347977015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32320.22980888603839913No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25590.18195573619191316No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA19400.13794221501067275No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18810.13374706517271928No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA18400.13083179155651434No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA16710.11881517591898667No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG14720.10466543324521148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21950.058.6908231
GTATCAA42550.047.0346531
ATCAACG53950.036.5711363
TATCAAC54300.036.4229662
TCAACGC54850.035.971064
CAACGCA56400.034.98255
AACGCAG58650.033.7035186
ACGCAGA67750.028.9723287
CGCAGAG68450.028.5361618
GTACTAG2053.092282E-1127.826771
TGGTATC8300.026.9216422
TAAGGTG6900.024.8047715
GCAGAGT78800.024.4847779
GTGGTAT10000.024.2440761
GTACATG47300.024.220961
GCGTAGA1601.8473565E-623.7686981
TACATGG45500.023.7189942
GAGTACT47800.023.11404212-13
GTACTTT50000.022.09034214-15
AGAGTAC68750.021.63516410-11
Produced by FastQC (version 0.11.5)