##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938307_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1406386 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.082544905879324 33.0 31.0 34.0 31.0 34.0 2 32.19943386808458 33.0 31.0 34.0 31.0 34.0 3 32.26105706399239 34.0 31.0 34.0 31.0 34.0 4 35.88542192541735 37.0 35.0 37.0 35.0 37.0 5 35.87206357287402 37.0 35.0 37.0 35.0 37.0 6 35.915737926856494 37.0 36.0 37.0 35.0 37.0 7 35.867665064925276 37.0 35.0 37.0 35.0 37.0 8 35.85011014045931 37.0 35.0 37.0 35.0 37.0 9 37.51769286668098 39.0 37.0 39.0 35.0 39.0 10-11 37.39628736349765 39.0 37.0 39.0 35.0 39.0 12-13 37.29797722673576 39.0 37.0 39.0 35.0 39.0 14-15 38.49759880999953 40.0 38.0 41.0 34.5 41.0 16-17 38.34566790340632 40.0 38.0 41.0 34.0 41.0 18-19 38.472884044636395 40.0 38.0 41.0 34.0 41.0 20-21 38.45583324919332 40.0 38.0 41.0 34.0 41.0 22-23 38.38956481364291 40.0 38.0 41.0 34.0 41.0 24-25 38.278938356894905 40.0 38.0 41.0 34.0 41.0 26-27 38.09926684423765 40.0 38.0 41.0 34.0 41.0 28-29 37.921599048909755 40.0 38.0 41.0 33.0 41.0 30-31 37.69119928668232 40.0 37.0 41.0 33.0 41.0 32-33 37.44030052915771 40.0 37.0 41.0 33.0 41.0 34-35 37.21087133973177 39.0 36.0 41.0 32.0 41.0 36-37 36.9735886876007 39.0 36.0 40.5 31.0 41.0 38-39 36.885045428495445 39.0 36.0 41.0 31.0 41.0 40-41 36.79453400417808 39.0 36.0 41.0 31.0 41.0 42-43 36.519081176860404 39.0 35.5 40.5 30.5 41.0 44-45 36.20828172350976 39.0 35.0 40.0 30.0 41.0 46-47 35.83269138060248 38.5 35.0 40.0 29.0 41.0 48-49 35.45788069562695 38.0 35.0 40.0 27.5 41.0 50-51 34.74848903501599 37.5 34.0 39.5 26.5 40.5 52-53 34.771970497431 38.0 34.0 39.5 27.0 40.5 54-55 34.87634511435694 38.0 34.0 40.0 26.5 41.0 56-57 34.56781850786342 38.0 34.0 40.0 26.0 41.0 58-59 34.20082466691221 37.0 34.0 40.0 25.5 41.0 60-61 33.73729971714735 37.0 33.0 39.5 24.0 41.0 62-63 33.20042612767761 36.0 32.5 39.0 22.5 40.5 64-65 32.70180199461599 35.5 32.0 39.0 21.5 40.0 66-67 32.191612402285 35.0 31.0 38.0 19.5 40.0 68-69 31.634800829928626 35.0 31.0 38.0 16.5 40.0 70-71 31.03019903497333 35.0 30.5 37.0 10.5 39.5 72-73 30.44082527840863 34.0 30.0 36.5 7.0 39.0 74-75 29.818998482635635 34.0 29.0 36.0 3.5 39.0 76-77 29.136817346020223 34.0 28.5 35.0 2.0 37.5 78-79 28.421386802769653 33.5 27.0 35.0 2.0 37.0 80-81 27.80889492642845 33.0 26.0 35.0 2.0 36.5 82-83 27.133734977452846 32.5 25.5 35.0 2.0 36.0 84-85 26.491886651317632 32.0 24.5 35.0 2.0 35.5 86-87 25.787518860398215 31.5 22.0 34.0 2.0 35.0 88-89 25.021169508228894 31.0 19.0 34.0 2.0 35.0 90-91 24.147295977064616 31.0 15.0 34.0 2.0 35.0 92-93 23.25021900104239 30.0 4.5 34.0 2.0 35.0 94-95 22.272848634727595 29.5 2.0 34.0 2.0 35.0 96-97 21.010652480897846 29.0 2.0 34.0 2.0 35.0 98-99 19.44767581588554 27.5 2.0 33.5 2.0 35.0 100-101 15.43124504936767 12.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25509647854211437 1101 2 0.2203460396860919 1101 3 0.14583252092417354 1101 4 0.09143143410778265 1101 5 0.0931650897147378 1101 6 0.21264903066823138 1101 7 0.2214435244871069 1101 8 0.3020661023081672 1101 9 0.4564166266475951 1101 10-11 0.3853967419617561 1101 12-13 0.45586142975239596 1101 14-15 0.5026171459216613 1101 16-17 0.34805614030447174 1101 18-19 0.3613246441007689 1101 20-21 0.42031786069347277 1101 22-23 0.42440601772799624 1101 24-25 0.4782203822182467 1101 26-27 0.355915902310187 1101 28-29 0.4512293967776557 1101 30-31 0.513982860327431 1101 32-33 0.3703803160009542 1101 34-35 0.36859785755346053 1101 36-37 0.351928686652478 1101 38-39 0.4810805441687478 1101 40-41 0.49620286010662085 1101 42-43 0.7015260372322771 1101 44-45 0.5478095689609859 1101 46-47 0.6498588322707448 1101 48-49 0.7450082528332445 1101 50-51 0.6859615833212658 1101 52-53 0.7138599920533508 1101 54-55 0.6580520830346828 1101 56-57 0.624815266169179 1101 58-59 0.5629922990204435 1101 60-61 0.6239450950074925 1101 62-63 0.5835384970343753 1101 64-65 0.6908816159367603 1101 66-67 0.6196228363465579 1101 68-69 0.674178765641475 1101 70-71 0.8385233672412653 1101 72-73 0.7026230648743628 1101 74-75 0.4709041443986024 1101 76-77 0.6172057685344292 1101 78-79 0.9922145886056661 1101 80-81 1.0228720493517294 1101 82-83 0.8229617180578614 1101 84-85 0.9060430777338269 1101 86-87 1.0835563713339909 1101 88-89 1.0554649785722603 1101 90-91 0.9948592507317002 1101 92-93 0.8578906488116651 1101 94-95 1.1487605885458478 1101 96-97 1.0960494242413645 1101 98-99 1.2098113133574664 1101 100-101 1.343508919991221 1102 1 0.23810173373050247 1102 2 0.18564212650061762 1102 3 0.10154048683967432 1102 4 0.08269616793397461 1102 5 0.08086624169953183 1102 6 0.1954115674100123 1102 7 0.06742234043675666 1102 8 0.22519375588139212 1102 9 0.278390068647532 1102 10-11 0.2550908944512926 1102 12-13 0.24606102299487276 1102 14-15 0.42837443489764127 1102 16-17 0.38269918831374383 1102 18-19 0.2871082832444998 1102 20-21 0.3403026072865032 1102 22-23 0.4240528883672354 1102 24-25 0.2920729623079197 1102 26-27 0.26135331509484416 1102 28-29 0.2355638509209399 1102 30-31 0.23015147171069827 1102 32-33 0.27354660411591425 1102 34-35 0.07466730323501025 1102 36-37 0.3863963761510121 1102 38-39 0.299528259497869 1102 40-41 0.40883535320361375 1102 42-43 0.4908570027618069 1102 44-45 0.40668085395846276 1102 46-47 0.3005884721270391 1102 48-49 0.49988451838355985 1102 50-51 0.5443149576272432 1102 52-53 0.49044434228048317 1102 54-55 0.5770188224426605 1102 56-57 0.37415855318559466 1102 58-59 0.4362487343994417 1102 60-61 0.4336720181986138 1102 62-63 0.5832685005972067 1102 64-65 0.49484824400599337 1102 66-67 0.18644908853621445 1102 68-69 0.44056856998220084 1102 70-71 0.58840367809902 1102 72-73 0.5787637302346518 1102 74-75 0.661382270048076 1102 76-77 0.6032375817228761 1102 78-79 0.6919854163720629 1102 80-81 0.8793976127257359 1102 82-83 1.0231560041538792 1102 84-85 0.9955868858155341 1102 86-87 1.0441541490128046 1102 88-89 0.9653538996256685 1102 90-91 0.9986779838453259 1102 92-93 1.0147359399253517 1102 94-95 1.0690927119982412 1102 96-97 1.3503548932001266 1102 98-99 1.5788700927880086 1102 100-101 1.1312028349581116 1103 1 0.26218731963071207 1103 2 0.20525022642843993 1103 3 0.14782578869527185 1103 4 0.13072036993160197 1103 5 0.1020720925528309 1103 6 0.27345689813571994 1103 7 0.14524503373842634 1103 8 0.17779817719871005 1103 9 0.2391229386621987 1103 10-11 0.23207847914081725 1103 12-13 0.2987969895614242 1103 14-15 0.44784793376844334 1103 16-17 0.49203453556991406 1103 18-19 0.45654773168574536 1103 20-21 0.4070800106065633 1103 22-23 0.42135286392076665 1103 24-25 0.3702863624615773 1103 26-27 0.37177129139342213 1103 28-29 0.42712152711008144 1103 30-31 0.3693653433318502 1103 32-33 0.3505405914503186 1103 34-35 0.46631417990842294 1103 36-37 0.6241796931867043 1103 38-39 0.6122814549997244 1103 40-41 0.6292551338839303 1103 42-43 0.4961661049945789 1103 44-45 0.5444825058408469 1103 46-47 0.46448162944584936 1103 48-49 0.5498731682403744 1103 50-51 0.7824700646096261 1103 52-53 0.704602627302954 1103 54-55 0.6887238758756382 1103 56-57 0.709637500125126 1103 58-59 0.7314426763332733 1103 60-61 0.8160539767094761 1103 62-63 0.8382109058962683 1103 64-65 0.872348185800135 1103 66-67 0.9309020698404922 1103 68-69 0.7682501140673246 1103 70-71 0.8892515920009068 1103 72-73 1.0338135253500162 1103 74-75 1.2064678035630116 1103 76-77 0.9783408971288026 1103 78-79 1.119997133212184 1103 80-81 1.1313984392489864 1103 82-83 0.8102964374383212 1103 84-85 0.9159467144574762 1103 86-87 1.222813406073172 1103 88-89 1.0229478729906702 1103 90-91 1.0985614266056807 1103 92-93 1.4305651697453783 1103 94-95 1.245806438104733 1103 96-97 1.2601634541183806 1103 98-99 1.2991349003594266 1103 100-101 1.1844721127397708 1104 1 0.2202166079332457 1104 2 0.08540078138649676 1104 3 0.10836956332417458 1104 4 0.053340463255388215 1104 5 0.04401431007125467 1104 6 0.14131889204268333 1104 7 0.13222447936595927 1104 8 0.2718664603922889 1104 9 0.25574490017577745 1104 10-11 0.38123961479453783 1104 12-13 0.27848720998782284 1104 14-15 0.469379070978988 1104 16-17 0.4591587516229225 1104 18-19 0.35096697889667183 1104 20-21 0.45440105787834284 1104 22-23 0.45530324977051606 1104 24-25 0.3097399812223145 1104 26-27 0.5342536342862232 1104 28-29 0.5203483750540059 1104 30-31 0.5064609080950433 1104 32-33 0.3762626582281072 1104 34-35 0.3761347451358148 1104 36-37 0.4129687334256289 1104 38-39 0.5860780579793996 1104 40-41 0.5235454788563132 1104 42-43 0.560735592942045 1104 44-45 0.3365333797337513 1104 46-47 0.38515033569586166 1104 48-49 0.3524251112005956 1104 50-51 0.47679036774770367 1104 52-53 0.43199278188566126 1104 54-55 0.5523626253882412 1104 56-57 0.6171587136486494 1104 58-59 0.7127641706565768 1104 60-61 0.7644554104165877 1104 62-63 0.822207210936817 1104 64-65 0.7693499411558236 1104 66-67 0.7624370205577051 1104 68-69 0.6830419851565175 1104 70-71 0.7414335748244163 1104 72-73 0.8961316517899007 1104 74-75 0.7435229123050853 1104 76-77 0.8028308584652741 1104 78-79 0.856730200599845 1104 80-81 0.9329550498271111 1104 82-83 1.1386252574079556 1104 84-85 1.072030185976864 1104 86-87 1.2279127214987362 1104 88-89 1.1556329101902136 1104 90-91 1.0048653887962615 1104 92-93 1.0875679177554254 1104 94-95 1.278994836760436 1104 96-97 1.5712268725914633 1104 98-99 1.8580080519870208 1104 100-101 1.6381101552539423 1105 1 0.14517688996969014 1105 2 0.12024507140756668 1105 3 0.02598644306964104 1105 4 0.08981485206560791 1105 5 0.08945795583370142 1105 6 0.21543728057561395 1105 7 0.1768373370928913 1105 8 0.15280629422053238 1105 9 0.28854705732451436 1105 10-11 0.24719750031342613 1105 12-13 0.27660405507291586 1105 14-15 0.3885687537870197 1105 16-17 0.22977071242415548 1105 18-19 0.2518499697053116 1105 20-21 0.3113788992865949 1105 22-23 0.3311748875370313 1105 24-25 0.3357356182588447 1105 26-27 0.3564358163055559 1105 28-29 0.2575548051907077 1105 30-31 0.4854814765535309 1105 32-33 0.44549835445607044 1105 34-35 0.3926486467767347 1105 36-37 0.4143183660963601 1105 38-39 0.47257469309332834 1105 40-41 0.5572251094474225 1105 42-43 0.49206843301340086 1105 44-45 0.5945880271245301 1105 46-47 0.3329951321828517 1105 48-49 0.35700204945843694 1105 50-51 0.4065102623801451 1105 52-53 0.45439258156282136 1105 54-55 0.4844910626013146 1105 56-57 0.5152761246174578 1105 58-59 0.6402215914434635 1105 60-61 0.5524152439901897 1105 62-63 0.6190399243916929 1105 64-65 0.6800060974755411 1105 66-67 0.8299441188616115 1105 68-69 0.5454563775600896 1105 70-71 0.5618394124808646 1105 72-73 0.5216031036636117 1105 74-75 0.5265632711319519 1105 76-77 0.7246213074798575 1105 78-79 0.6851340972751352 1105 80-81 0.8001264022467609 1105 82-83 0.9106918930453318 1105 84-85 0.7968863807766766 1105 86-87 0.889407446755115 1105 88-89 0.9648314207842645 1105 90-91 1.020821718134929 1105 92-93 1.0973877686733609 1105 94-95 1.2447846901824065 1105 96-97 1.493828303711389 1105 98-99 1.6632068257170012 1105 100-101 1.634430673207314 1106 1 0.07415885564566338 1106 2 -0.10226690765699686 1106 3 -0.14229604857579403 1106 4 -0.006039320204969556 1106 5 0.011878220778854143 1106 6 -0.11249844507531037 1106 7 0.006843279134429281 1106 8 0.004136338915017745 1106 9 -0.2508071901713933 1106 10-11 -0.18820084442527474 1106 12-13 -0.10892635191893874 1106 14-15 0.11868305070369445 1106 16-17 -0.06968614546682517 1106 18-19 -0.1546103667874874 1106 20-21 -0.12051633841785758 1106 22-23 -0.11088602826682603 1106 24-25 -0.23492946487655075 1106 26-27 -0.1731495857244667 1106 28-29 -0.16075884038095012 1106 30-31 -0.3638262224174724 1106 32-33 -0.26503968904697217 1106 34-35 -0.41950077657168805 1106 36-37 -0.1781563650300626 1106 38-39 -0.0635442951487235 1106 40-41 0.09825900947826938 1106 42-43 -0.22420136302716998 1106 44-45 -0.2024847955579645 1106 46-47 -0.13484888694717512 1106 48-49 -0.032741546130161225 1106 50-51 -0.29751585068884623 1106 52-53 -0.3507540470504651 1106 54-55 -0.16218599124026412 1106 56-57 -0.6776394345651227 1106 58-59 -0.3927690866939244 1106 60-61 -0.004799321458136774 1106 62-63 -0.13140629861267428 1106 64-65 -0.16548258605071453 1106 66-67 -0.3657276129775475 1106 68-69 -0.29283479919208943 1106 70-71 -0.2859314861047473 1106 72-73 -0.3556399790249891 1106 74-75 0.011119092129089125 1106 76-77 0.10868710001294346 1106 78-79 0.36789708580289116 1106 80-81 0.3947950156538873 1106 82-83 0.23458368710116062 1106 84-85 0.06648846915352635 1106 86-87 -0.14547507254891912 1106 88-89 0.025872980121853573 1106 90-91 0.08708250993095845 1106 92-93 0.24934028401097308 1106 94-95 0.3266271941678305 1106 96-97 0.4008936722471397 1106 98-99 0.6694284927407992 1106 100-101 0.3831216384167373 1107 1 0.14114652850123832 1107 2 -0.019388874172264536 1107 3 0.07547228535933215 1107 4 0.04509283235769601 1107 5 0.005869846428936398 1107 6 0.114911951914479 1107 7 0.13865179347914847 1107 8 0.09257576107220444 1107 9 0.2720757843064803 1107 10-11 0.22743961597392826 1107 12-13 0.255379248506749 1107 14-15 0.3371177060694208 1107 16-17 0.3702027930431413 1107 18-19 0.3752889763138043 1107 20-21 0.3370132917044586 1107 22-23 0.31502183963129227 1107 24-25 0.35727350542047986 1107 26-27 0.19180907304927786 1107 28-29 0.32279830252144137 1107 30-31 0.2514794540234959 1107 32-33 0.4540654448349457 1107 34-35 0.39556632274861414 1107 36-37 0.369814620888107 1107 38-39 0.3450259524710475 1107 40-41 0.1969193193672254 1107 42-43 0.39687870323282226 1107 44-45 0.2413559476814271 1107 46-47 0.1734857133074712 1107 48-49 0.16664831248141354 1107 50-51 0.2628246423335838 1107 52-53 0.17692600622357446 1107 54-55 0.3135791896444218 1107 56-57 0.38831312398248485 1107 58-59 0.2940992342552491 1107 60-61 0.2837332985984986 1107 62-63 0.46260619164787187 1107 64-65 0.45569487478557846 1107 66-67 0.37469406594658494 1107 68-69 0.4145063763841996 1107 70-71 0.6398251430610493 1107 72-73 0.5887037715688841 1107 74-75 0.6712682404412327 1107 76-77 0.753623888557442 1107 78-79 0.7705264622242183 1107 80-81 1.0867987229823974 1107 82-83 0.9409651697791794 1107 84-85 0.8467896638603278 1107 86-87 0.8870960888378505 1107 88-89 0.8647308822268442 1107 90-91 0.8508630506529791 1107 92-93 1.0041140039453431 1107 94-95 1.2966833108639513 1107 96-97 1.7457156064559172 1107 98-99 1.7108362323631283 1107 100-101 1.8677962455127624 1108 1 0.36617378474799267 1108 2 0.2614186079843961 1108 3 0.21563991127685256 1108 4 0.18272895512490095 1108 5 0.21345468942028134 1108 6 0.25099906414683204 1108 7 0.19624725372939622 1108 8 0.3286124809413735 1108 9 0.3236138606493242 1108 10-11 0.37721269187306206 1108 12-13 0.35435587152115744 1108 14-15 0.3968944583925378 1108 16-17 0.49736189979888934 1108 18-19 0.5033774893904663 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-1.3747587474765233 2316 80-81 -1.6962428185313065 2316 82-83 -1.9263356052421408 2316 84-85 -1.9970588361582138 2316 86-87 -1.955008381440635 2316 88-89 -2.3487244520706305 2316 90-91 -2.251848133386776 2316 92-93 -2.1884396264176935 2316 94-95 -2.3883008827801326 2316 96-97 -2.652229103745441 2316 98-99 -2.5289475774785295 2316 100-101 -2.453292956104029 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3448.0 3 915.0 4 795.0 5 843.0 6 967.0 7 1209.0 8 1390.0 9 1759.0 10 2358.0 11 3151.0 12 4263.0 13 5577.0 14 6579.0 15 8379.0 16 10679.0 17 13740.0 18 12596.0 19 10917.0 20 11412.0 21 12437.0 22 13574.0 23 15413.0 24 17661.0 25 20250.0 26 23399.0 27 28030.0 28 33346.0 29 39669.0 30 48901.0 31 61446.0 32 77754.0 33 100290.0 34 133823.0 35 175519.0 36 212857.0 37 201634.0 38 85320.0 39 4086.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.134937649813594 17.72463239450741 14.108271807988304 25.03215814769069 2 15.672583955569094 18.864560886113065 39.77210941480897 25.69074574350887 3 19.352331606217614 22.312674372259863 28.990633718612997 29.34436030290953 4 13.169117940133127 15.175617658279384 34.82082358233261 36.83444081925488 5 14.646276220813748 36.13256970026642 33.40892294416432 15.81223113475551 6 34.58381187815857 34.114660051718424 16.251595742409815 15.04993232771319 7 30.076118395646397 31.61029202068987 19.39557598718156 18.918013596482183 8 27.495947922897095 33.976426476936766 18.803202403295998 19.724423196870138 9 26.51105797882705 16.154221432665736 19.09021473060178 38.24450585790544 10-11 25.708062629394618 25.375482234459394 26.422198203119805 22.494256933026183 12-13 26.368299880529513 22.607807088772013 27.084624870134977 23.939268160563497 14-15 22.991180488831773 24.1821807612189 25.132370898813516 27.694267851135812 16-17 23.038834450839506 27.295101642387355 25.939336395140227 23.726727511632912 18-19 23.127107071224344 25.633472737876783 28.133828361712116 23.105591829186757 20-21 23.140519972199478 24.946380134435582 28.67163585584419 23.241464037520746 22-23 22.94763202055702 24.736840795126454 28.76353846137433 23.55198872294219 24-25 22.795794226824803 25.075942708557474 28.685555396298057 23.442707668319667 26-27 22.39766214993427 25.366640677908226 28.48048993552132 23.755207236636185 28-29 22.565372030160763 25.941883624982214 28.263515436050646 23.229228908806373 30-31 22.549653609250747 25.391743820243907 28.197373719264846 23.861228851240504 32-33 22.2193018240949 25.38944338910978 28.36322275852429 24.028032028271035 34-35 23.101934106976124 25.51961228936019 28.150922591258727 23.227531012404963 36-37 23.211643006140534 25.137099456012407 28.06101054720635 23.590246990640708 38-39 22.618480328076394 25.429832135384146 28.097053087664847 23.85463444887462 40-41 22.962612345575398 25.27437160625056 28.279701319821577 23.48331472835246 42-43 22.795608606687086 25.36110717961228 28.098082321555196 23.745201892145435 44-45 22.567486609341195 25.755538924018566 28.155742294926927 23.52123217171331 46-47 23.02713661606707 26.042454452007185 27.53580212147814 23.394606810447602 48-49 22.85183200708533 26.18977440925047 27.266973882614877 23.691419701049323 50-51 22.944492068238358 26.275593739922414 27.305832103430582 23.474082088408647 52-53 23.262477666865184 25.838988097883576 27.41102745753371 23.48750677771753 54-55 22.791721836880107 26.23935647471936 27.53041058016546 23.43851110823507 56-57 22.75087086519039 26.366245880346305 27.3293977136522 23.553485540811106 58-59 22.78993177908456 25.94425128736487 27.28868619445628 23.977130739094292 60-61 22.34579499736505 26.251103229573452 27.60726662916338 23.795835143898117 62-63 22.651066427592898 26.250612884312684 27.73223737707203 23.36608331102239 64-65 22.43489255097611 26.381072218273637 27.454122479194098 23.729912751556153 66-67 22.294086776953534 26.344227859190827 27.541969121194192 23.81971624266145 68-69 22.92896 25.906915555555553 27.614222222222224 23.549902222222222 70-71 22.65848059898403 26.124259671170424 27.236064215469252 23.981195514376292 72-73 22.593603948023944 26.384523393386033 27.172784010474583 23.84908864811544 74-75 22.50520072116667 26.669155900729347 27.226246483956885 23.599396894147098 76-77 22.676326386054047 26.39192128092032 27.10310117102881 23.828651161996824 78-79 22.796306007626193 26.599376038824253 27.179393282195775 23.424924671353782 80-81 22.91602818946472 26.48895253711582 26.994982013608364 23.6000372598111 82-83 22.73143572276777 26.63187160908179 26.981505052157033 23.655187615993402 84-85 22.659357326542125 26.760923923535646 26.93342984942707 23.646288900495154 86-87 22.961754587074413 26.75287327713595 26.538257501952025 23.74711463383761 88-89 22.79286302084037 26.769930859269703 26.425009584110075 24.012196535779847 90-91 22.695267242243826 26.73965813874591 26.570064629742767 23.995009989267494 92-93 22.938000531268568 26.35700181036699 26.674660587304245 24.030337071060192 94-95 22.725906715665474 26.13731782376148 26.97689603946839 24.159879421104648 96-97 23.05505113766092 25.915047254058045 26.79013820931766 24.239763398963376 98-99 22.854869095649466 26.126535538292718 26.47405631024185 24.544539055815967 100-101 23.367291618642692 25.5625563837023 26.737934243440993 24.332217754214014 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 551.0 1 395.0 2 342.5 3 477.0 4 428.0 5 410.0 6 703.5 7 1444.5 8 2342.0 9 2535.5 10 1972.0 11 1525.0 12 1427.0 13 1449.0 14 1522.5 15 1629.0 16 1698.0 17 1813.5 18 2010.5 19 2190.0 20 2436.5 21 2896.0 22 3426.0 23 3973.0 24 4767.0 25 5889.0 26 7261.0 27 8907.0 28 10337.0 29 11589.0 30 13073.5 31 15682.0 32 19800.5 33 21027.0 34 20392.0 35 21382.5 36 23208.5 37 25844.0 38 28601.5 39 30379.0 40 32309.5 41 35532.5 42 37578.0 43 40023.0 44 42696.5 45 45798.0 46 48370.0 47 50048.5 48 52088.0 49 52291.0 50 51472.5 51 50830.0 52 50959.5 53 52649.5 54 54320.5 55 52540.0 56 49543.0 57 46552.0 58 44461.0 59 41130.0 60 34205.0 61 28420.0 62 24217.0 63 18699.0 64 14013.5 65 11322.5 66 8683.5 67 6519.0 68 5020.0 69 3908.5 70 3081.0 71 2256.5 72 1707.5 73 1434.5 74 1143.0 75 912.0 76 696.0 77 483.0 78 336.0 79 229.5 80 153.5 81 103.0 82 60.0 83 33.5 84 21.5 85 15.5 86 13.0 87 9.5 88 7.5 89 5.5 90 4.5 91 4.5 92 2.5 93 1.5 94 1.5 95 2.5 96 2.5 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003910732899787114 2 0.08802704236248085 3 0.09570629969297192 4 0.09968813682730061 5 0.13310712706184505 6 0.07707699024307693 7 0.020406915313434575 8 0.022540042349682092 9 0.017918267104479142 10-11 0.03985392346055777 12-13 0.04262698860767954 14-15 0.02126016612793358 16-17 0.025135346910449906 18-19 0.028868319223883058 20-21 0.046715482093820614 22-23 0.02456651303411723 24-25 0.03956950652239143 26-27 0.046217752452029526 28-29 0.04166708144136816 30-31 0.05318596743710475 32-33 0.04020944463326569 34-35 0.04966630782729635 36-37 0.04006723616418252 38-39 0.02645077524946921 40-41 0.02847724593390435 42-43 0.02581083713859495 44-45 0.006292724756930174 46-47 0.01063008306396679 48-49 0.0071459755714291815 50-51 0.007785913682303436 52-53 0.00878137296588561 54-55 0.009385758959489074 56-57 0.01205216775479847 58-59 0.015571827364606872 60-61 0.020406915313434575 62-63 0.008923581434968778 64-65 0.008141434855011355 66-67 0.008105882737740563 68-69 0.009670175897655408 70-71 0.024068783392326147 72-73 0.022433385997869718 74-75 0.023535501633264268 76-77 0.019340351795310816 78-79 0.0034485553752668183 80-81 0.0031996905543712753 82-83 0.003981837134328698 84-85 0.004088493486141073 86-87 0.010807843650320751 88-89 0.02936604886567415 90-91 0.026806296422177127 92-93 0.022575594466952884 94-95 0.03565877362260432 96-97 0.03377451140725235 98-99 0.02012249837526824 100-101 0.0595853485458473 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1406386.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.76768741838959 #Duplication Level Percentage of deduplicated Percentage of total 1 77.32019622912786 43.119685344345484 2 12.808903390851917 14.286458409467606 3 4.117168897508179 6.888149643748556 4 1.9080015932600305 4.256193457868588 5 1.0317975707692162 2.8770482202855687 6 0.6292875824025317 2.1056347915019082 7 0.43192156514299207 1.6861086783909185 8 0.28817275492526684 1.2856582495334776 9 0.2240172240870824 1.1243630276301373 >10 1.1157203105652496 11.051535175393138 >50 0.07530672578563476 2.92392213378034 >100 0.043015708926212835 4.3372768256174945 >500 0.004454228091631428 1.723767935335976 >1k 0.0019089548964134689 1.9131212312630343 >5k 1.2726365976089795E-4 0.42107687583779424 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5933 0.4218614235352173 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4287 0.30482385347977015 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3232 0.22980888603839913 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2559 0.18195573619191316 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1940 0.13794221501067275 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1881 0.13374706517271928 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1840 0.13083179155651434 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1671 0.11881517591898667 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1472 0.10466543324521148 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 3.555211727079194E-5 14-15 0.0 0.0 0.0 0.0 7.110423454158388E-5 16-17 0.0 0.0 0.0 0.0 7.110423454158388E-5 18-19 0.0 0.0 0.0 0.0 7.110423454158388E-5 20-21 0.0 0.0 0.0 0.0 7.110423454158388E-5 22-23 0.0 0.0 0.0 7.110423454158388E-5 7.110423454158388E-5 24-25 7.110423454158388E-5 0.0 0.0 2.8441693816633553E-4 7.110423454158388E-5 26-27 7.110423454158388E-5 0.0 0.0 4.2662540724950336E-4 7.110423454158388E-5 28-29 7.110423454158388E-5 0.0 0.0 0.0010310114008529664 7.110423454158388E-5 30-31 7.110423454158388E-5 0.0 0.0 0.003093034202558899 7.110423454158388E-5 32-33 7.110423454158388E-5 0.0 0.0 0.009385758959489073 7.110423454158388E-5 34-35 7.110423454158388E-5 0.0 0.0 0.02086909283795487 1.4220846908316777E-4 36-37 1.4220846908316777E-4 0.0 0.0 0.03942729805330827 1.4220846908316777E-4 38-39 1.4220846908316777E-4 0.0 0.0 0.06822451304264975 1.4220846908316777E-4 40-41 1.4220846908316777E-4 0.0 0.0 0.1028167231471303 1.4220846908316777E-4 42-43 1.4220846908316777E-4 0.0 0.0 0.1409285928614193 1.4220846908316777E-4 44-45 2.1331270362475168E-4 0.0 0.0 0.19219474596590125 1.4220846908316777E-4 46-47 2.8441693816633553E-4 0.0 0.0 0.25476647236249506 1.4220846908316777E-4 48-49 2.8441693816633553E-4 0.0 0.0 0.31751595934544286 1.4220846908316777E-4 50-51 2.8441693816633553E-4 0.0 0.0 0.3896867574051505 1.4220846908316777E-4 52-53 2.8441693816633553E-4 0.0 0.0 0.46139537794033786 2.1331270362475168E-4 54-55 2.8441693816633553E-4 0.0 0.0 0.5440896027122 2.1331270362475168E-4 56-57 2.8441693816633553E-4 0.0 0.0 0.6448087509403535 2.1331270362475168E-4 58-59 2.8441693816633553E-4 0.0 0.0 0.7591088079659496 2.1331270362475168E-4 60-61 3.5552117270791944E-4 0.0 0.0 0.8827946239510348 2.1331270362475168E-4 62-63 3.5552117270791944E-4 0.0 0.0 1.006978169577911 2.1331270362475168E-4 64-65 3.5552117270791944E-4 0.0 0.0 1.1403697135779223 2.1331270362475168E-4 66-67 4.2662540724950336E-4 0.0 0.0 1.3057937152389174 2.1331270362475168E-4 68-69 4.2662540724950336E-4 0.0 0.0 1.4876783471962889 2.1331270362475168E-4 70-71 4.2662540724950336E-4 0.0 0.0 1.6735448162879893 2.1331270362475168E-4 72-73 4.2662540724950336E-4 0.0 0.0 1.8728855378253195 2.1331270362475168E-4 74-75 4.2662540724950336E-4 0.0 0.0 2.0814342577357854 2.1331270362475168E-4 76-77 4.2662540724950336E-4 0.0 0.0 2.3154382936121376 2.1331270362475168E-4 78-79 4.2662540724950336E-4 0.0 0.0 2.576568594966105 2.1331270362475168E-4 80-81 5.688338763326711E-4 0.0 0.0 2.855226090134572 2.1331270362475168E-4 82-83 5.688338763326711E-4 0.0 0.0 3.1442292514288397 2.1331270362475168E-4 84-85 5.688338763326711E-4 0.0 0.0 3.4423693068617007 2.1331270362475168E-4 86-87 5.688338763326711E-4 0.0 0.0 3.7544457922647125 2.1331270362475168E-4 88-89 5.688338763326711E-4 0.0 0.0 4.090875477998217 2.1331270362475168E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2195 0.0 58.690823 1 GTATCAA 4255 0.0 47.034653 1 ATCAACG 5395 0.0 36.571136 3 TATCAAC 5430 0.0 36.422966 2 TCAACGC 5485 0.0 35.97106 4 CAACGCA 5640 0.0 34.9825 5 AACGCAG 5865 0.0 33.703518 6 ACGCAGA 6775 0.0 28.972328 7 CGCAGAG 6845 0.0 28.536161 8 GTACTAG 205 3.092282E-11 27.82677 1 TGGTATC 830 0.0 26.921642 2 TAAGGTG 690 0.0 24.804771 5 GCAGAGT 7880 0.0 24.484777 9 GTGGTAT 1000 0.0 24.244076 1 GTACATG 4730 0.0 24.22096 1 GCGTAGA 160 1.8473565E-6 23.768698 1 TACATGG 4550 0.0 23.718994 2 GAGTACT 4780 0.0 23.114042 12-13 GTACTTT 5000 0.0 22.090342 14-15 AGAGTAC 6875 0.0 21.635164 10-11 >>END_MODULE