FastQCFastQC Report
Thu 26 May 2016
SRR938307_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938307_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1406386
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61330.43608227044353404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53480.38026544632839065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41280.2935182801876583No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC17650.12549897396589557No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA16510.11739309122815501No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG15920.11319794139020155No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA15050.10701187298508374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29900.060.875591
GTATCAA50850.038.972721
GTGGTAT10400.031.5305371
GTAAGGT7900.030.0647534
TGGTATC10500.029.8713462
AAGGTAA7800.029.2457141
TATCAAC69350.028.5067042
TAAGGTG9500.028.5013895
ATCAACG70050.028.0741713
AGGTAAG8300.028.0555082
TCAACGC71050.027.6790394
CAACGCA72750.026.9669485
AACGCAG75350.026.0253286
GTATAGG2202.1573214E-923.7621441
GGTAAGG10900.023.5332573
TATAGGG3400.022.3635732
ACGCAGA87300.022.1365327
GTACATG48950.021.8446661
CGCAGAG88300.021.778298
TACATGG48150.021.7133352
Produced by FastQC (version 0.11.5)