##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938307_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1406386 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.07129266076312 31.0 31.0 33.0 30.0 34.0 2 31.330282724657383 31.0 31.0 34.0 30.0 34.0 3 31.348029630556617 31.0 31.0 34.0 28.0 34.0 4 35.14507325869285 35.0 35.0 37.0 32.0 37.0 5 35.00731520364964 35.0 35.0 37.0 32.0 37.0 6 35.05894541043497 36.0 35.0 37.0 32.0 37.0 7 34.98805093338529 36.0 35.0 37.0 32.0 37.0 8 34.96175303224008 36.0 35.0 37.0 32.0 37.0 9 36.40758084906989 38.0 35.0 39.0 32.0 39.0 10-11 36.23451527532271 38.0 35.0 39.0 32.0 39.0 12-13 36.089070141483205 38.0 35.0 39.0 31.0 39.0 14-15 36.817005430941435 38.0 36.0 40.0 31.0 41.0 16-17 36.63952926152564 38.0 36.0 40.0 31.0 41.0 18-19 36.93405153350503 38.5 36.0 40.0 31.0 41.0 20-21 36.87141474673383 39.0 36.0 40.0 31.0 41.0 22-23 36.68188001018213 38.0 35.0 40.0 30.0 41.0 24-25 36.45393049987699 38.0 35.0 40.0 30.0 41.0 26-27 36.109835066617556 38.0 34.5 40.0 29.0 41.0 28-29 35.909282728923635 38.0 34.0 40.0 29.0 41.0 30-31 35.49837846793128 38.0 34.0 40.0 27.0 41.0 32-33 35.16806516845304 38.0 33.5 40.0 27.0 41.0 34-35 34.94514201648765 38.0 33.0 40.0 26.0 41.0 36-37 34.7967275698137 38.0 33.0 40.0 26.0 41.0 38-39 34.49017588343456 37.0 33.0 40.0 25.0 41.0 40-41 34.15966349209961 37.0 33.0 40.0 24.0 41.0 42-43 33.78178430388243 37.0 32.0 39.0 24.0 40.0 44-45 33.28096696070638 36.5 31.5 39.0 22.5 40.0 46-47 32.63642022887031 36.0 31.0 39.0 19.5 40.0 48-49 32.00520163027789 35.5 30.0 39.0 16.5 40.0 50-51 31.35209857037826 35.0 29.5 38.5 13.0 40.0 52-53 30.532867576895676 34.5 28.0 38.0 8.5 40.0 54-55 29.88002760266385 34.0 27.0 38.0 7.5 40.0 56-57 29.265528809302708 34.0 26.0 38.0 4.0 39.5 58-59 28.45722369249978 33.0 25.5 37.0 2.0 39.0 60-61 27.743162261285306 32.5 23.5 36.5 2.0 39.0 62-63 26.953758783150572 31.5 22.5 36.0 2.0 39.0 64-65 26.07894312087862 31.0 20.5 35.5 2.0 38.0 66-67 25.243648614249572 30.5 18.5 35.0 2.0 38.0 68-69 24.416849997084725 30.0 14.5 34.0 2.0 37.5 70-71 23.49346054354921 29.0 8.0 34.0 2.0 36.5 72-73 22.69208702305057 28.5 7.0 34.0 2.0 36.0 74-75 21.871368884502548 27.5 4.5 33.0 2.0 35.5 76-77 21.172149751206284 26.5 2.0 31.5 2.0 34.5 78-79 21.624910586425063 28.0 2.0 33.0 2.0 35.0 80-81 20.96121583974812 27.0 2.0 33.0 2.0 35.0 82-83 20.18429897624123 26.0 2.0 33.0 2.0 35.0 84-85 19.253243064137443 25.5 2.0 32.0 2.0 34.5 86-87 18.242732080666332 24.0 2.0 32.0 2.0 34.0 88-89 17.074043683597534 19.5 2.0 31.0 2.0 34.0 90-91 16.06405496072913 14.5 2.0 31.0 2.0 34.0 92-93 14.874422455854937 4.5 2.0 30.0 2.0 34.0 94-95 13.703210925023429 2.0 2.0 29.5 2.0 34.0 96-97 12.376476301669669 2.0 2.0 29.0 2.0 33.5 98-99 10.947959877302534 2.0 2.0 26.0 2.0 33.0 100-101 8.71278866541618 2.0 2.0 12.5 2.0 30.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.300819586543696 1101 2 0.3466350323871268 1101 3 0.3648311224413163 1101 4 0.40018432483918787 1101 5 0.28171047267348825 1101 6 0.2964383859944064 1101 7 0.41095653432092405 1101 8 0.2323295365898872 1101 9 0.2684507896980577 1101 10-11 0.4324240583362524 1101 12-13 0.485577099354785 1101 14-15 0.5933704903405967 1101 16-17 0.5588212728142778 1101 18-19 0.5762709937695902 1101 20-21 0.5466042260448987 1101 22-23 0.47069528615751466 1101 24-25 0.678639950525401 1101 26-27 0.6780268760134049 1101 28-29 0.634662414700621 1101 30-31 0.6584637256425268 1101 32-33 0.7434744773448401 1101 34-35 1.0213754608707362 1101 36-37 0.8436464630459 1101 38-39 0.7802997345361931 1101 40-41 1.02153450131744 1101 42-43 1.1293907416426237 1101 44-45 1.069943074580955 1101 46-47 0.933980727199824 1101 48-49 0.9545181488178223 1101 50-51 1.060391798022426 1101 52-53 1.1829871829240055 1101 54-55 1.1532850622828974 1101 56-57 0.9055991839022433 1101 58-59 1.06624094729208 1101 60-61 1.3674415277296355 1101 62-63 1.4246917362317006 1101 64-65 1.4363761052076818 1101 66-67 1.2246510860581807 1101 68-69 1.5352864108279825 1101 70-71 1.429681688464374 1101 72-73 1.5834234197970218 1101 74-75 1.3989037606352248 1101 76-77 1.186706121726516 1101 78-79 1.7440880473920224 1101 80-81 1.8083004463421943 1101 82-83 2.190829816451238 1101 84-85 2.2191565582518002 1101 86-87 2.2819079684600077 1101 88-89 2.3964158964693887 1101 90-91 2.3858569112917607 1101 92-93 2.340245867387294 1101 94-95 1.9626797673204965 1101 96-97 1.9825625395920916 1101 98-99 1.9085124755851535 1101 100-101 1.7826931901122922 1102 1 0.2465014991947463 1102 2 0.313651673817013 1102 3 0.2126069456057138 1102 4 0.3596659316798423 1102 5 0.2710924684574607 1102 6 0.31432421183092885 1102 7 0.2877472323405854 1102 8 0.3905708299006605 1102 9 0.24029285198837158 1102 10-11 0.34999405328890276 1102 12-13 0.5300747389735605 1102 14-15 0.663539131414197 1102 16-17 0.5276679519508818 1102 18-19 0.5554836804475372 1102 20-21 0.6040832201365234 1102 22-23 0.3388119543086958 1102 24-25 0.44237859397421886 1102 26-27 0.5833102702119177 1102 28-29 0.5309702366180247 1102 30-31 0.4480171830814399 1102 32-33 0.3972512728675355 1102 34-35 0.7278537052815324 1102 36-37 0.8440452412888249 1102 38-39 0.9263900747873777 1102 40-41 0.7580777304377122 1102 42-43 0.8823213627643227 1102 44-45 0.8488874349027995 1102 46-47 0.9255507147298871 1102 48-49 0.9690684516591865 1102 50-51 1.1330340147024494 1102 52-53 1.0402388604276425 1102 54-55 1.149808881350026 1102 56-57 1.1172016081176714 1102 58-59 1.304667656712823 1102 60-61 1.3131000592459223 1102 62-63 1.4070074335090972 1102 64-65 1.2980939505804443 1102 66-67 1.3310476820618646 1102 68-69 1.4214117485554887 1102 70-71 1.3737590243510773 1102 72-73 1.5451965634091493 1102 74-75 1.8503251851772475 1102 76-77 1.9774726147047517 1102 78-79 2.2810617862952576 1102 80-81 2.2130516413779695 1102 82-83 2.3908821456576135 1102 84-85 2.13246524861135 1102 86-87 1.8202613798789073 1102 88-89 2.0775545577324124 1102 90-91 2.018676230789403 1102 92-93 2.3378446619599043 1102 94-95 2.4899210160957708 1102 96-97 2.374557284403707 1102 98-99 2.2959556928292972 1102 100-101 1.811264936794398 1103 1 0.3065364809181048 1103 2 0.28056018584234366 1103 3 0.3333535522039277 1103 4 0.25977885105307763 1103 5 0.1510865314594909 1103 6 0.2511839473434918 1103 7 0.30734951168215474 1103 8 0.18878565609795572 1103 9 0.27064866442579927 1103 10-11 0.33579182284024967 1103 12-13 0.4605798494482869 1103 14-15 0.6240406331254533 1103 16-17 0.5886938884832134 1103 18-19 0.5178870587157931 1103 20-21 0.6337623894224507 1103 22-23 0.5390939617124815 1103 24-25 0.7013139214404092 1103 26-27 0.5423246121364329 1103 28-29 0.7007851128723601 1103 30-31 0.6534635056350453 1103 32-33 0.770983112638433 1103 34-35 0.7727180565189897 1103 36-37 0.9958410376614353 1103 38-39 0.807300152550404 1103 40-41 1.1015781728022702 1103 42-43 1.0412095434818838 1103 44-45 1.1182787179928297 1103 46-47 1.1247915647683016 1103 48-49 1.3578479620314745 1103 50-51 1.399193417277477 1103 52-53 1.3790660996071722 1103 54-55 1.4554180348039623 1103 56-57 1.350156748859451 1103 58-59 1.6157750814526395 1103 60-61 1.4529512850613848 1103 62-63 1.763893769100818 1103 64-65 1.7723225773877331 1103 66-67 1.7536942735265555 1103 68-69 1.9172980492236924 1103 70-71 1.8661187773253936 1103 72-73 1.858583475238909 1103 74-75 1.6137212644303496 1103 76-77 1.7864591633299298 1103 78-79 2.0580882233980056 1103 80-81 2.064917721329543 1103 82-83 2.1234596758664566 1103 84-85 2.142988868550347 1103 86-87 2.2522922115242707 1103 88-89 2.3414926790799946 1103 90-91 2.3614723822177766 1103 92-93 2.348243139294537 1103 94-95 1.984861471498439 1103 96-97 1.8621623459855101 1103 98-99 1.8212228577381477 1103 100-101 1.6649033852589277 1104 1 0.26193594931242004 1104 2 0.3470616005533387 1104 3 0.3922247399470038 1104 4 0.3067189047278305 1104 5 0.33779089042615595 1104 6 0.13365545673514134 1104 7 0.41388167725404656 1104 8 0.19926922873954567 1104 9 0.20683955685777278 1104 10-11 0.36170135132228864 1104 12-13 0.4246714707313757 1104 14-15 0.4829141061159632 1104 16-17 0.553026174887556 1104 18-19 0.44692066327943536 1104 20-21 0.5491889522817601 1104 22-23 0.511464784530105 1104 24-25 0.4931685854161927 1104 26-27 0.5217025749240349 1104 28-29 0.6200050079509367 1104 30-31 0.7639111213364131 1104 32-33 0.8197177996952263 1104 34-35 0.8003029570321729 1104 36-37 0.65433580230237 1104 38-39 0.8518992558764396 1104 40-41 0.8604368988053821 1104 42-43 1.1197927757644308 1104 44-45 1.3089733332357127 1104 46-47 1.3272882333060139 1104 48-49 1.351483904974561 1104 50-51 1.6440694640279254 1104 52-53 1.8703484162196418 1104 54-55 1.7009982234225731 1104 56-57 1.7819319362140327 1104 58-59 1.8695944797755573 1104 60-61 1.9502330903497302 1104 62-63 2.2442581375226958 1104 64-65 2.1303278348851293 1104 66-67 2.1847019391034443 1104 68-69 2.7056818151115145 1104 70-71 2.3027029822663856 1104 72-73 1.951870489463147 1104 74-75 2.2046933944095137 1104 76-77 2.187212830795005 1104 78-79 2.6091881415575777 1104 80-81 2.345654120893631 1104 82-83 2.377350845241857 1104 84-85 2.718622346282757 1104 86-87 2.8083927152785044 1104 88-89 2.8252529609081343 1104 90-91 2.898822252172913 1104 92-93 3.125208702043862 1104 94-95 2.919399308768826 1104 96-97 2.5019570022200224 1104 98-99 2.1984873951255093 1104 100-101 1.7593869407611624 1105 1 0.12681564950722546 1105 2 0.1913512679410374 1105 3 0.1674345059682345 1105 4 0.17302640028210448 1105 5 0.27625528007104805 1105 6 0.24652591604881025 1105 7 0.20428754320468556 1105 8 0.16441892620395748 1105 9 0.21282271615828563 1105 10-11 0.20739264194743612 1105 12-13 0.31335772216199587 1105 14-15 0.3758370582181598 1105 16-17 0.2892619376068595 1105 18-19 0.22497293395858975 1105 20-21 0.2479126369204181 1105 22-23 0.13009859268704105 1105 24-25 0.21115972650657255 1105 26-27 0.24473821569539211 1105 28-29 0.13640502549333178 1105 30-31 0.44886756243711545 1105 32-33 0.41345820619779516 1105 34-35 0.5791605509794735 1105 36-37 0.6071994568643149 1105 38-39 0.5960126504855552 1105 40-41 0.7971072602211606 1105 42-43 0.8893557296208598 1105 44-45 0.9390420598134384 1105 46-47 0.9104680117342454 1105 48-49 1.0727332984179476 1105 50-51 1.2170702322150753 1105 52-53 1.2497370213021206 1105 54-55 1.0873153672942628 1105 56-57 1.0053990633214198 1105 58-59 1.3048867374959805 1105 60-61 1.3633906295477907 1105 62-63 1.362954900243409 1105 64-65 1.5509585759523041 1105 66-67 1.5190845741126893 1105 68-69 1.6930560651387516 1105 70-71 1.5826431825043379 1105 72-73 1.86206881048615 1105 74-75 2.048555819589076 1105 76-77 1.6514638341775765 1105 78-79 1.8549597523031451 1105 80-81 2.1570793839234597 1105 82-83 2.1069693052176106 1105 84-85 2.188757479314944 1105 86-87 2.5353619552542277 1105 88-89 1.9780580111373887 1105 90-91 2.3210581577778484 1105 92-93 2.3623817096733912 1105 94-95 2.223061885039341 1105 96-97 1.9371970925350652 1105 98-99 1.9104225193865005 1105 100-101 1.5751390921803274 1106 1 -0.020989214456498928 1106 2 0.01156309944236611 1106 3 0.039239898788849814 1106 4 -0.035184027012988395 1106 5 -0.21452999643144466 1106 6 -0.09507992447652214 1106 7 -0.015170591237428255 1106 8 -0.007410553920593088 1106 9 -0.14514691408260205 1106 10-11 0.17088631922011643 1106 12-13 0.02089321104308084 1106 14-15 0.07157166779867197 1106 16-17 0.2326501246459145 1106 18-19 0.001835216887918989 1106 20-21 0.11918518362471531 1106 22-23 0.05703066800963086 1106 24-25 0.21303276133969717 1106 26-27 0.122767935021308 1106 28-29 0.2670114024740755 1106 30-31 0.15033069518474917 1106 32-33 0.18739202006144495 1106 34-35 -0.09990917758624107 1106 36-37 0.130062259532302 1106 38-39 0.16749913348082401 1106 40-41 0.26914642146628864 1106 42-43 0.43815417587577343 1106 44-45 0.11810380993463099 1106 46-47 -0.76153718413277 1106 48-49 -0.27720982667038996 1106 50-51 0.4716111069261366 1106 52-53 0.39999810640097166 1106 54-55 0.5002002512572865 1106 56-57 0.4205163920470092 1106 58-59 0.3103838857719481 1106 60-61 0.436881031858956 1106 62-63 0.574814706055669 1106 64-65 0.6768164487014303 1106 66-67 0.369904568246632 1106 68-69 0.3576158525625921 1106 70-71 0.2591791850452836 1106 72-73 0.8614314847391782 1106 74-75 0.8220367617445916 1106 76-77 0.8297402774022089 1106 78-79 0.729980268303347 1106 80-81 0.3232540465112379 1106 82-83 1.0521955739042639 1106 84-85 1.1411972116647249 1106 86-87 1.4906602502986246 1106 88-89 1.7771339932839076 1106 90-91 1.9213749400179836 1106 92-93 1.444872937183673 1106 94-95 1.1346696479898757 1106 96-97 1.2700260741967995 1106 98-99 1.2678085833994608 1106 100-101 0.8727217754603949 1107 1 0.09388927195260521 1107 2 0.11285623019498203 1107 3 0.09376502500588657 1107 4 0.05235073414066704 1107 5 -0.07774921182706862 1107 6 -0.027440183055162493 1107 7 -0.026927621067088126 1107 8 0.025796245646120042 1107 9 0.19560622313130693 1107 10-11 0.14046615268330243 1107 12-13 0.17368115058560107 1107 14-15 0.3499336810633409 1107 16-17 0.28758996963808414 1107 18-19 0.4631184973272866 1107 20-21 0.3355759385831263 1107 22-23 0.36547493614086335 1107 24-25 0.3514462907924525 1107 26-27 0.37791579265736885 1107 28-29 0.43406309444749525 1107 30-31 0.4201736523691437 1107 32-33 0.3469322543151989 1107 34-35 0.4713351529408598 1107 36-37 0.6043097082695041 1107 38-39 0.582595015669817 1107 40-41 0.6655443266118937 1107 42-43 0.6693629613416547 1107 44-45 0.5169249000570844 1107 46-47 0.8577697861515929 1107 48-49 1.1406806068067432 1107 50-51 1.0413717538161684 1107 52-53 0.9658192218618424 1107 54-55 1.1680578239627373 1107 56-57 0.8988781677639288 1107 58-59 0.9767694542712206 1107 60-61 1.0864076387902806 1107 62-63 1.4369837624526731 1107 64-65 1.1606426144274096 1107 66-67 0.948090241900374 1107 68-69 1.2511633826994846 1107 70-71 1.2057455907261598 1107 72-73 1.282345703080395 1107 74-75 1.3361225513216333 1107 76-77 1.5661339383613928 1107 78-79 1.7697819871988436 1107 80-81 1.796182709339135 1107 82-83 1.8643333135899418 1107 84-85 1.8988727752863497 1107 86-87 2.0441451153398305 1107 88-89 1.9260087296059076 1107 90-91 2.059917339123537 1107 92-93 1.9375034142428955 1107 94-95 2.0081158877674046 1107 96-97 1.7512154599299983 1107 98-99 1.5557124937521092 1107 100-101 1.2450580733896128 1108 1 0.15764432703557674 1108 2 0.09818777391273414 1108 3 0.26161913436048323 1108 4 0.12684269037854534 1108 5 0.328858526411544 1108 6 0.32678722912267943 1108 7 0.30298706054191626 1108 8 0.21213547816091705 1108 9 0.08547593193317482 1108 10-11 0.2690811708752676 1108 12-13 0.33977073489614185 1108 14-15 0.4744860130570956 1108 16-17 0.3804192789774774 1108 18-19 0.577639896650318 1108 20-21 0.5298381024755727 1108 22-23 0.5587526265859637 1108 24-25 0.449012561504027 1108 26-27 0.3856889731444255 1108 28-29 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-1.799814604764073 2316 68-69 -2.212494745753812 2316 70-71 -2.320928214294792 2316 72-73 -2.4699267502885363 2316 74-75 -2.4525728912563345 2316 76-77 -2.82055859325132 2316 78-79 -2.8812254941672713 2316 80-81 -3.193283582795697 2316 82-83 -3.4656162544005653 2316 84-85 -3.298291814768163 2316 86-87 -3.419153777751724 2316 88-89 -2.9240510656994623 2316 90-91 -2.6187165003840107 2316 92-93 -2.84801221597181 2316 94-95 -2.4574195351569106 2316 96-97 -2.135210542220779 2316 98-99 -2.2379612556453043 2316 100-101 -1.9534345286795842 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 24.0 4 77.0 5 143.0 6 320.0 7 771.0 8 1728.0 9 2911.0 10 4658.0 11 7036.0 12 9746.0 13 13345.0 14 17787.0 15 23014.0 16 28110.0 17 33701.0 18 33654.0 19 32923.0 20 34731.0 21 37032.0 22 39463.0 23 42159.0 24 45786.0 25 50212.0 26 56465.0 27 64242.0 28 71897.0 29 79092.0 30 85518.0 31 91941.0 32 97048.0 33 100465.0 34 100811.0 35 92011.0 36 68129.0 37 33420.0 38 5924.0 39 88.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.57164117445038 21.234775597154957 15.939182736919047 21.254400491475614 2 18.899473714534057 22.402163467864863 35.74829058582778 22.9500722317733 3 21.970355222534923 24.24177999496582 29.82132927944391 23.96653550305535 4 16.46930501299074 18.13421066478193 33.49194317918409 31.90454114304323 5 14.624932157949807 37.35313929973489 33.31547922628409 14.70644931603121 6 34.10507499363617 35.96217539139326 15.382547892257175 14.550201722713393 7 29.425207588812746 32.78694469370429 19.27002970734919 18.517818010133773 8 27.652223500518353 35.27893480168318 18.13549054100368 18.933351156794792 9 26.749981868420193 16.213187560171956 18.8771077072724 38.159722864135446 10-11 26.25065238135192 26.370711881375385 25.995992565341236 21.382643171931463 12-13 26.99909555413663 23.33093475048813 26.856496011763486 22.813473683611754 14-15 23.19167710713844 24.828283273582073 25.1267077459531 26.853331873326386 16-17 22.89979422434524 28.01901469440111 26.077264705422266 23.003926375831384 18-19 23.154631800942273 26.018177086518214 28.65642860494914 22.17076250759038 20-21 23.309567928008384 25.183946654759076 29.248691326563264 22.257794090669275 22-23 23.383978509456153 25.00184871009808 29.255055155554732 22.359117624891034 24-25 23.36585331166915 25.425064797004936 29.007548095554693 22.201533795771216 26-27 22.807847727019013 25.667019110916122 28.987591450039073 22.537541712025792 28-29 22.885901482914893 25.892900630730338 28.942988959997102 22.278208926357674 30-31 22.921263308188237 25.67999482355652 28.777079447741745 22.621662420513506 32-33 22.681588159128772 25.603516254107166 28.827318020766747 22.887577565997315 34-35 23.385177373603486 25.707528218285407 28.632217591371212 22.275076816739897 36-37 23.519145859994687 25.286253604283214 28.696797152771826 22.49780338295027 38-39 23.055118054247 25.59876843875436 28.613213685119938 22.7328998218787 40-41 23.291098377854894 25.577076156952057 28.663549677416146 22.468275787776903 42-43 23.18594031752533 25.700630556835442 28.460027759290547 22.653401366348685 44-45 23.17083273335573 25.742150070841802 28.519041955766983 22.567975240035484 46-47 23.634265415042528 26.10755510933698 27.80755070087444 22.45062877474605 48-49 23.477587234230146 26.30483380807261 27.41118014542238 22.806398812274868 50-51 23.482221389509668 26.404793580118046 27.473636233709076 22.63934879666321 52-53 24.02910722945194 26.108621672855104 27.362438192644124 22.499832905048827 54-55 23.585731081723427 26.53050776412918 27.34746828985087 22.536292864296527 56-57 23.530062159321837 26.501508120814627 27.264812078618526 22.703617641245007 58-59 23.477866634426995 26.255160422007844 27.186116553531487 23.080856390033674 60-61 23.207924424731193 26.382053006784766 27.542154145447977 22.867868423036068 62-63 23.363854636914056 26.4392614444222 27.54124251441119 22.65564140425255 64-65 23.158650777706864 26.470656210721316 27.33037385752915 23.04031915404267 66-67 22.971434544490062 26.57905702424921 27.30723721729287 23.142271213967863 68-69 23.622487366608024 26.17412675633661 27.379401419718757 22.823984457336614 70-71 23.40880220963598 26.322548120696638 26.984102154068 23.284547515599385 72-73 23.40811839708302 26.508227470978806 26.800536979179256 23.283117152758916 74-75 23.32231017046788 26.744753330061943 26.74212199441019 23.190814505059986 76-77 23.366141333207192 26.399669086513033 27.13590180149388 23.0982877787859 78-79 23.315993818085868 26.611924152778343 27.128304589424033 22.943777439711756 80-81 23.528453740735326 26.525330519838658 26.798181185216635 23.148034554209378 82-83 23.554541943550834 26.5770395306311 26.80335966622403 23.065058859594032 84-85 23.42355604197735 26.66263448602497 26.720557080957224 23.193252391040453 86-87 23.69617676977597 26.51959081491774 26.399662650927976 23.384569764378313 88-89 23.369021131626003 26.7976183784453 26.16521361093303 23.668146878995667 90-91 23.300852112002428 26.647924446045412 26.44107490641632 23.610148535535842 92-93 23.53269995156827 26.30116712644806 26.401373156873998 23.76475976510967 94-95 23.35751135411614 26.039152320819742 26.571405407019995 24.031930918044118 96-97 23.49479407274322 25.79449203400843 26.500813865117816 24.209900028130534 98-99 23.35584534574695 26.153497589929493 26.003472834777856 24.4871842295457 100-101 23.785928281039 25.649729526773186 26.369592935482515 24.194749256705297 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 539.0 1 389.0 2 335.0 3 477.0 4 430.5 5 404.0 6 711.5 7 1553.0 8 2347.0 9 2514.0 10 2026.5 11 1491.0 12 1421.5 13 1493.5 14 1635.5 15 1783.0 16 1930.5 17 2099.5 18 2315.5 19 2578.0 20 2860.5 21 3290.0 22 3940.5 23 4679.5 24 5386.5 25 6497.5 26 8065.5 27 9760.0 28 11264.0 29 12604.5 30 14376.5 31 17326.5 32 20893.5 33 21795.5 34 21300.5 35 22300.5 36 24266.5 37 26617.5 38 29248.5 39 31180.5 40 32969.5 41 35770.0 42 37748.0 43 40061.5 44 42984.5 45 45226.0 46 47255.0 47 49626.5 48 50745.5 49 50784.0 50 50963.0 51 50419.5 52 50098.0 53 50994.5 54 52375.5 55 51138.5 56 48154.0 57 45220.0 58 42266.0 59 38393.0 60 32752.5 61 27723.5 62 23537.5 63 18775.5 64 14392.5 65 11278.0 66 8862.0 67 6865.5 68 5281.0 69 4196.0 70 3392.0 71 2526.0 72 1874.5 73 1491.5 74 1174.0 75 954.0 76 741.0 77 525.5 78 362.0 79 243.5 80 149.5 81 98.0 82 58.5 83 31.0 84 20.5 85 12.0 86 7.5 87 4.0 88 2.0 89 0.0 90 0.0 91 1.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.39938110874255E-4 2 0.035196596098084024 3 0.0 4 0.0 5 0.03889401629424639 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0050484006524524555 26-27 0.001208771987206926 28-29 3.555211727079194E-5 30-31 0.0010665635181237583 32-33 2.1331270362475168E-4 34-35 0.008319195441365316 36-37 0.01752719381450043 38-39 0.0029152736162049394 40-41 5.332817590618791E-4 42-43 7.110423454158389E-4 44-45 0.005937203584222255 46-47 0.0 48-49 0.0 50-51 3.5552117270791944E-4 52-53 0.0 54-55 0.0010310114008529664 56-57 0.0 58-59 0.017847162869937556 60-61 0.0 62-63 0.0071459755714291815 64-65 0.011981063520256886 66-67 0.00511950488699404 68-69 0.013652013031984107 70-71 3.555211727079194E-5 72-73 0.0 74-75 0.018202684042645474 76-77 0.041276008151389446 78-79 0.02481537785501278 80-81 0.021722343652453877 82-83 0.02936604886567415 84-85 0.014149742673775193 86-87 0.009065789904051946 88-89 0.06804675245629578 90-91 0.020726884368871705 92-93 0.02012249837526824 94-95 0.02047801954797616 96-97 0.0313214153155677 98-99 0.04394241694669884 100-101 0.010452322477612833 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1406386.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.01657954496927 #Duplication Level Percentage of deduplicated Percentage of total 1 78.90001494400863 45.77508993098341 2 11.956413627111335 13.873404445397187 3 3.95961311471386 6.891696277113006 4 1.735670496973662 4.027906618061153 5 0.9195485762788346 2.667453156057213 6 0.5777539919532264 2.011158625894674 7 0.36860997038237486 1.4969842767430472 8 0.2683480434439738 1.245490848656334 9 0.19777188920579794 1.0326643687680324 >10 1.0028242038231499 10.318290025866695 >50 0.06947660017857153 2.7589095540143767 >100 0.03842959455646168 4.214901025401333 >500 0.0038060748549165976 1.4983396376200009 >1k 0.0014733192986773926 1.3699105258240007 >5k 2.455532164462321E-4 0.8178006835994879 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6133 0.43608227044353404 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5348 0.38026544632839065 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4128 0.2935182801876583 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1765 0.12549897396589557 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1651 0.11739309122815501 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1592 0.11319794139020155 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1505 0.10701187298508374 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 3.555211727079194E-5 14-15 0.0 0.0 0.0 0.0 7.110423454158388E-5 16-17 0.0 0.0 0.0 0.0 7.110423454158388E-5 18-19 0.0 0.0 0.0 0.0 7.110423454158388E-5 20-21 0.0 0.0 0.0 0.0 1.0665635181237583E-4 22-23 0.0 0.0 0.0 7.110423454158388E-5 1.4220846908316777E-4 24-25 7.110423454158388E-5 0.0 0.0 2.8441693816633553E-4 1.4220846908316777E-4 26-27 7.110423454158388E-5 0.0 0.0 4.2662540724950336E-4 1.4220846908316777E-4 28-29 1.4220846908316777E-4 0.0 0.0 0.0011021156353945502 1.4220846908316777E-4 30-31 2.1331270362475168E-4 0.0 0.0 0.003164138437100483 1.4220846908316777E-4 32-33 2.1331270362475168E-4 0.0 0.0 0.009350206842218281 1.4220846908316777E-4 34-35 2.1331270362475168E-4 0.0 0.0 0.020584675899788536 1.4220846908316777E-4 36-37 2.1331270362475168E-4 0.0 0.0 0.03860959935608005 1.4220846908316777E-4 38-39 2.1331270362475168E-4 0.0 0.0 0.06651801141365173 1.4220846908316777E-4 40-41 2.1331270362475168E-4 0.0 0.0 0.10022141858636249 1.4220846908316777E-4 42-43 2.1331270362475168E-4 0.0 0.0 0.13726672478252772 1.4220846908316777E-4 44-45 2.1331270362475168E-4 0.0 0.0 0.18618643814713742 1.4220846908316777E-4 46-47 2.1331270362475168E-4 3.555211727079194E-5 0.0 0.24594954727933865 1.4220846908316777E-4 48-49 2.1331270362475168E-4 7.110423454158388E-5 0.0 0.3056060000597276 1.4220846908316777E-4 50-51 2.1331270362475168E-4 7.110423454158388E-5 0.0 0.3731550228742323 1.4220846908316777E-4 52-53 2.1331270362475168E-4 7.110423454158388E-5 0.0 0.4408818062750909 1.7776058635395972E-4 54-55 2.1331270362475168E-4 7.110423454158388E-5 0.0 0.5170344414691272 2.1331270362475168E-4 56-57 2.8441693816633553E-4 7.110423454158388E-5 0.0 0.6084744870896042 2.1331270362475168E-4 58-59 3.5552117270791944E-4 7.110423454158388E-5 0.0 0.7117889398785255 2.1331270362475168E-4 60-61 3.5552117270791944E-4 7.110423454158388E-5 0.0 0.824133630454228 2.1331270362475168E-4 62-63 4.2662540724950336E-4 7.110423454158388E-5 0.0 0.936087247739952 2.1331270362475168E-4 64-65 4.2662540724950336E-4 7.110423454158388E-5 0.0 1.0540491728444397 2.8441693816633553E-4 66-67 4.2662540724950336E-4 7.110423454158388E-5 0.0 1.2002394790619362 2.8441693816633553E-4 68-69 4.2662540724950336E-4 7.110423454158388E-5 0.0 1.3605795279532078 2.8441693816633553E-4 70-71 4.2662540724950336E-4 7.110423454158388E-5 0.0 1.5260035296142025 2.8441693816633553E-4 72-73 4.2662540724950336E-4 7.110423454158388E-5 0.0 1.704155189258141 3.1996905543712746E-4 74-75 4.2662540724950336E-4 7.110423454158388E-5 0.0 1.8885284694244682 3.5552117270791944E-4 76-77 4.2662540724950336E-4 7.110423454158388E-5 0.0 2.0997435981302432 3.9107328997871143E-4 78-79 4.2662540724950336E-4 7.110423454158388E-5 0.0 2.3293036193477468 4.977296417910872E-4 80-81 5.688338763326711E-4 7.110423454158388E-5 0.0 2.573795529818983 4.977296417910872E-4 82-83 5.688338763326711E-4 7.110423454158388E-5 0.0 2.822411485893631 4.977296417910872E-4 84-85 6.04385993603463E-4 7.110423454158388E-5 0.0 3.07376495499813 5.332817590618791E-4 86-87 6.39938110874255E-4 7.110423454158388E-5 0.0 3.3283536667742712 6.04385993603463E-4 88-89 6.75490228145047E-4 7.110423454158388E-5 0.0 3.6002918117785585 6.39938110874255E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2990 0.0 60.87559 1 GTATCAA 5085 0.0 38.97272 1 GTGGTAT 1040 0.0 31.530537 1 GTAAGGT 790 0.0 30.064753 4 TGGTATC 1050 0.0 29.871346 2 AAGGTAA 780 0.0 29.245714 1 TATCAAC 6935 0.0 28.506704 2 TAAGGTG 950 0.0 28.501389 5 ATCAACG 7005 0.0 28.074171 3 AGGTAAG 830 0.0 28.055508 2 TCAACGC 7105 0.0 27.679039 4 CAACGCA 7275 0.0 26.966948 5 AACGCAG 7535 0.0 26.025328 6 GTATAGG 220 2.1573214E-9 23.762144 1 GGTAAGG 1090 0.0 23.533257 3 TATAGGG 340 0.0 22.363573 2 ACGCAGA 8730 0.0 22.136532 7 GTACATG 4895 0.0 21.844666 1 CGCAGAG 8830 0.0 21.77829 8 TACATGG 4815 0.0 21.713335 2 >>END_MODULE