FastQCFastQC Report
Thu 26 May 2016
SRR938306_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938306_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511381
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61540.40717727694075817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46400.3070039917135388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34540.2285327127971041No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26810.17738743572930982No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21140.13987207725914247No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA20320.1344465756814463No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20210.1337187644941944No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA16990.11241374610372898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23750.054.1021041
GTATCAA44250.045.385091
TATCAAC58050.034.6364942
ATCAACG58600.034.0669333
TCAACGC59200.033.8822444
CAACGCA60700.032.9644665
GTGGTAT11800.032.264281
AACGCAG63350.031.6563556
TGGTATC11300.031.5484922
ACGCAGA72900.027.2449677
CGCAGAG73850.026.7658088
TACATGG55200.026.694292
GTACATG56300.026.3730411
TAAGGTG6550.024.6711985
ACATGGG56600.024.4375673
GCAGAGT83250.023.3402089
ACCGAGT4100.023.1784138
GAGTACT51850.021.5298812-13
CATGGGG38600.021.4261114
GGACCGA4450.021.3582216
Produced by FastQC (version 0.11.5)