##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938306_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1511381 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9864428625211 33.0 31.0 34.0 31.0 34.0 2 32.153405395462826 33.0 31.0 34.0 31.0 34.0 3 32.23782289177911 34.0 31.0 34.0 31.0 34.0 4 35.85501736491328 37.0 35.0 37.0 35.0 37.0 5 35.86940751537832 37.0 35.0 37.0 35.0 37.0 6 35.91972044110651 37.0 35.0 37.0 35.0 37.0 7 35.87624430901275 37.0 35.0 37.0 35.0 37.0 8 35.847866950821796 37.0 35.0 37.0 35.0 37.0 9 37.51635755643348 39.0 37.0 39.0 35.0 39.0 10-11 37.40572496279892 39.0 37.0 39.0 35.0 39.0 12-13 37.30882682791434 39.0 37.0 39.0 35.0 39.0 14-15 38.519236049679066 40.0 38.0 41.0 34.5 41.0 16-17 38.38575051558806 40.0 38.0 41.0 34.0 41.0 18-19 38.3688795214443 40.0 38.0 41.0 34.0 41.0 20-21 38.4517891253099 40.0 38.0 41.0 34.0 41.0 22-23 38.393418998915564 40.0 38.0 41.0 34.0 41.0 24-25 38.30166847406445 40.0 38.0 41.0 34.0 41.0 26-27 38.137850416274915 40.0 38.0 41.0 34.0 41.0 28-29 37.98107426254531 40.0 38.0 41.0 33.5 41.0 30-31 37.77292886439621 40.0 37.5 41.0 33.0 41.0 32-33 37.519114637540106 40.0 37.0 41.0 33.0 41.0 34-35 37.30390417770238 39.5 36.5 41.0 32.0 41.0 36-37 37.05224526443035 39.0 36.0 41.0 31.5 41.0 38-39 36.80746317440804 39.0 36.0 40.0 31.0 41.0 40-41 36.77367487086313 39.0 36.0 40.5 31.0 41.0 42-43 36.60689065166228 39.0 36.0 41.0 30.5 41.0 44-45 36.35108420709272 39.0 35.0 40.0 30.0 41.0 46-47 36.003795866164786 39.0 35.0 40.0 29.5 41.0 48-49 35.63930504617962 38.0 35.0 40.0 28.5 41.0 50-51 34.88221467651108 37.5 34.0 39.5 26.5 40.5 52-53 34.93711810589123 38.0 34.0 40.0 27.0 40.5 54-55 35.06162410404789 38.0 34.0 40.0 27.0 41.0 56-57 34.76914027634329 38.0 34.0 40.0 26.5 41.0 58-59 34.43368349873394 37.5 34.0 40.0 26.0 41.0 60-61 34.00321063980559 37.0 33.5 40.0 25.5 41.0 62-63 33.49200730987091 36.0 33.0 39.0 23.5 41.0 64-65 33.01026114527045 36.0 32.5 39.0 22.0 40.0 66-67 32.491963310376406 35.0 32.0 38.5 20.5 40.0 68-69 31.959381850109267 35.0 31.0 38.0 19.0 40.0 70-71 31.36232657417289 35.0 31.0 37.0 17.0 39.5 72-73 30.85837290530978 34.5 30.0 36.5 11.5 39.0 74-75 30.3056317368023 34.0 30.0 36.0 7.5 39.0 76-77 29.644292537751895 34.0 29.0 35.5 4.0 38.0 78-79 28.93259277442286 34.0 28.5 35.0 2.0 37.0 80-81 28.355358774524756 33.0 27.0 35.0 2.0 36.5 82-83 27.77008378430058 33.0 26.0 35.0 2.0 36.0 84-85 27.17780791210158 33.0 25.5 35.0 2.0 35.5 86-87 26.58286229613843 32.0 24.5 35.0 2.0 35.0 88-89 25.84534508505797 32.0 23.0 34.0 2.0 35.0 90-91 25.080849236559146 31.0 19.5 34.0 2.0 35.0 92-93 24.311015554648364 31.0 16.5 34.0 2.0 35.0 94-95 23.405022294179957 30.5 4.5 34.0 2.0 35.0 96-97 22.185353329173783 30.0 2.0 34.0 2.0 35.0 98-99 20.711087409461943 29.0 2.0 34.0 2.0 35.0 100-101 16.882115429531005 22.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34371243513621863 1101 2 0.3896706930128815 1101 3 0.3719372475345466 1101 4 0.31291395103860964 1101 5 0.20814566681026037 1101 6 0.24743200264551035 1101 7 0.27826244871613426 1101 8 0.2957394969556688 1101 9 0.44090798775587814 1101 10-11 0.31544069172655753 1101 12-13 0.37515731588572265 1101 14-15 0.3984517913269414 1101 16-17 0.3184839020547301 1101 18-19 0.33916861019143596 1101 20-21 0.47561157217020167 1101 22-23 0.5137798988049411 1101 24-25 0.4393273203486814 1101 26-27 0.4907970057577842 1101 28-29 0.4380232456331896 1101 30-31 0.3231088370001487 1101 32-33 0.2942657265348032 1101 34-35 0.30704312708694914 1101 36-37 0.1749641210135664 1101 38-39 0.13665794018729116 1101 40-41 0.0016439853891512257 1101 42-43 0.20788014897819806 1101 44-45 -0.11269320216609913 1101 46-47 0.07804105086526647 1101 48-49 0.06922701927280173 1101 50-51 -0.10757993890486262 1101 52-53 -0.07075063346340471 1101 54-55 -0.004097303387759155 1101 56-57 -0.07468344899690038 1101 58-59 -0.019519451196764237 1101 60-61 -0.003117680139233414 1101 62-63 0.0891504812484527 1101 64-65 -0.26535044474211134 1101 66-67 -0.26773792278110164 1101 68-69 -0.36698078537973444 1101 70-71 -0.2118341610553962 1101 72-73 -0.014934955726189969 1101 74-75 -0.1931794734959098 1101 76-77 0.03659851639558198 1101 78-79 0.035364890311193165 1101 80-81 -0.14845196009704154 1101 82-83 -0.03047859301221223 1101 84-85 0.007587859324733159 1101 86-87 0.02131333008319558 1101 88-89 0.10803046772518954 1101 90-91 0.011312102969970539 1101 92-93 0.23566695011082217 1101 94-95 -0.034052053278710304 1101 96-97 0.15770217208956083 1101 98-99 0.19617232456524647 1101 100-101 0.30214019613524457 1102 1 0.4771557770820678 1102 2 0.4749597098832794 1102 3 0.41815986298930596 1102 4 0.37232402985399204 1102 5 0.40116871433832557 1102 6 0.37253988526921233 1102 7 0.40936629829383264 1102 8 0.3499854584816049 1102 9 0.3997124311629605 1102 10-11 0.4912208391525823 1102 12-13 0.5240363543597866 1102 14-15 0.5213623288131046 1102 16-17 0.5117077876747231 1102 18-19 0.4861960028787564 1102 20-21 0.4076716417578936 1102 22-23 0.4407844122339526 1102 24-25 0.35507675854482557 1102 26-27 0.45647710053164303 1102 28-29 0.3498739822204584 1102 30-31 0.36391255576168646 1102 32-33 0.4166931669890275 1102 34-35 0.5603905417036188 1102 36-37 0.4755237536427046 1102 38-39 0.7014112621578903 1102 40-41 0.7433027587569256 1102 42-43 0.8581480914190891 1102 44-45 0.9301772491225222 1102 46-47 0.973228671639518 1102 48-49 0.7566435629035198 1102 50-51 0.8148745061306357 1102 52-53 0.729945718339799 1102 54-55 0.8986831507234498 1102 56-57 0.7221538938608703 1102 58-59 0.8853375737940112 1102 60-61 0.8794995794521441 1102 62-63 0.7409094429698015 1102 64-65 0.79036618085226 1102 66-67 0.9854405435757982 1102 68-69 0.9698989913100142 1102 70-71 1.1231593659871884 1102 72-73 1.0518281041778401 1102 74-75 1.0312699858696632 1102 76-77 0.9597677686265129 1102 78-79 0.9694842288482235 1102 80-81 0.9854700685848101 1102 82-83 0.8786228329382411 1102 84-85 0.7472012731380531 1102 86-87 0.7797783842551986 1102 88-89 1.117561117246435 1102 90-91 1.009977140314998 1102 92-93 1.0735342938370742 1102 94-95 1.3016610305087717 1102 96-97 1.4962039199237687 1102 98-99 1.3909136912413196 1102 100-101 1.098179614681829 1103 1 0.3097265323554552 1103 2 0.3770871519461352 1103 3 0.2339897252927443 1103 4 0.3264631838576264 1103 5 0.27670575190932567 1103 6 0.27683120537899697 1103 7 0.18044150059218111 1103 8 0.24495807906767197 1103 9 0.26803304685245166 1103 10-11 0.20744841726058638 1103 12-13 0.2318541665755376 1103 14-15 0.31878087866093807 1103 16-17 0.3862703592688135 1103 18-19 0.5500648097444483 1103 20-21 0.3855289175520724 1103 22-23 0.3506525349419576 1103 24-25 0.4428845312052587 1103 26-27 0.5013746210498269 1103 28-29 0.42130227279611887 1103 30-31 0.49628396164020216 1103 32-33 0.5422960645672106 1103 34-35 0.5874839069468365 1103 36-37 0.6548068166769525 1103 38-39 0.6403931123194937 1103 40-41 0.7239861169488648 1103 42-43 0.7369361886481229 1103 44-45 0.6238092745176118 1103 46-47 0.599303729766703 1103 48-49 0.6992139643022384 1103 50-51 0.7450687964284413 1103 52-53 0.7664282303780894 1103 54-55 0.8767458704206348 1103 56-57 0.7786791460948663 1103 58-59 0.8240304263121487 1103 60-61 0.8055699192504591 1103 62-63 0.8297192051922337 1103 64-65 0.6609312793735924 1103 66-67 0.7967545719539331 1103 68-69 0.7896634156345748 1103 70-71 0.7442094038648825 1103 72-73 0.844472950321002 1103 74-75 0.7372168496226728 1103 76-77 0.71201192379662 1103 78-79 0.541465940081256 1103 80-81 0.6491859229562138 1103 82-83 0.6203660850726322 1103 84-85 0.4308697607301575 1103 86-87 0.45078962698438474 1103 88-89 0.5304306213762864 1103 90-91 0.6200249527142425 1103 92-93 0.7981575483830525 1103 94-95 0.8148084573156922 1103 96-97 0.9365144383894695 1103 98-99 1.052550859614886 1103 100-101 0.8750465656715001 1104 1 0.37692949554529065 1104 2 0.27251283930535664 1104 3 0.3286506876275652 1104 4 0.3105752797210286 1104 5 0.27741592208218435 1104 6 0.35168620432618525 1104 7 0.2776942969271161 1104 8 0.19875235304149896 1104 9 0.3149081066713677 1104 10-11 0.23756987358798654 1104 12-13 0.22737030013602322 1104 14-15 0.3187805596267168 1104 16-17 0.3359809952316226 1104 18-19 0.348792341759534 1104 20-21 0.3657331632594705 1104 22-23 0.39245686487439 1104 24-25 0.4612221395971332 1104 26-27 0.43069067548982787 1104 28-29 0.3991463033468392 1104 30-31 0.22196972569525286 1104 32-33 0.3265362526697899 1104 34-35 0.3139096708742741 1104 36-37 0.5037244930869278 1104 38-39 0.46719246531809233 1104 40-41 0.3489130581125792 1104 42-43 0.41714713407412773 1104 44-45 0.3856966490574081 1104 46-47 0.5085920920364444 1104 48-49 0.31419788497757395 1104 50-51 0.24163439305434053 1104 52-53 0.45316310656090764 1104 54-55 0.6214647374663116 1104 56-57 0.5231542709967627 1104 58-59 0.39981620759505176 1104 60-61 0.251097378525742 1104 62-63 0.33196600368384566 1104 64-65 0.10510252418130506 1104 66-67 0.10412617663224921 1104 68-69 0.12566610828339364 1104 70-71 0.14001282774012225 1104 72-73 0.09386247834177652 1104 74-75 0.13929073156725025 1104 76-77 0.27855751697694586 1104 78-79 0.10341807856762841 1104 80-81 0.21157641446033182 1104 82-83 0.17569890157052725 1104 84-85 -0.003171350019382402 1104 86-87 0.09485463339697375 1104 88-89 0.2795886900707316 1104 90-91 0.41139220576580726 1104 92-93 0.33626714301817273 1104 94-95 0.5457501506217213 1104 96-97 0.498980381486529 1104 98-99 0.21666030749426923 1104 100-101 0.5666789361595548 1105 1 0.3349500786394799 1105 2 0.43034678489694045 1105 3 0.41537387523408853 1105 4 0.2706709976522532 1105 5 0.19082951888908894 1105 6 0.2991909014219303 1105 7 0.25161334174192973 1105 8 0.3147090805600925 1105 9 0.3690691259177754 1105 10-11 0.3871155873128558 1105 12-13 0.37994677010109257 1105 14-15 0.39106379995288165 1105 16-17 0.38681323023909187 1105 18-19 0.43892797332028266 1105 20-21 0.39593151623803635 1105 22-23 0.4020903511943885 1105 24-25 0.4934535638687265 1105 26-27 0.4137259808244167 1105 28-29 0.45661865825098147 1105 30-31 0.4627152786439055 1105 32-33 0.3792602566206469 1105 34-35 0.45013090018914426 1105 36-37 0.4980878224008549 1105 38-39 0.407848282706226 1105 40-41 0.33503426966727545 1105 42-43 0.6367945481009158 1105 44-45 0.5323608542278038 1105 46-47 0.582030677704303 1105 48-49 0.44324650108697483 1105 50-51 0.3506942572669871 1105 52-53 0.2900370997621948 1105 54-55 0.3556318637161482 1105 56-57 0.4981241254569042 1105 58-59 0.504070701383668 1105 60-61 0.38789690367507035 1105 62-63 0.6156775973313131 1105 64-65 0.7309711363647793 1105 66-67 0.6893104616922088 1105 68-69 0.6124765387959705 1105 70-71 0.7150464006798636 1105 72-73 0.7407126978511585 1105 74-75 0.4844451670900689 1105 76-77 0.5721903427435144 1105 78-79 0.675082829006179 1105 80-81 0.7458242403982709 1105 82-83 0.7314243730716967 1105 84-85 0.8197297570307818 1105 86-87 0.9472714176857728 1105 88-89 0.7108107450773851 1105 90-91 0.7101884094530675 1105 92-93 0.7820779256182 1105 94-95 0.7471317043555601 1105 96-97 0.8353497679507917 1105 98-99 0.8456501904329805 1105 100-101 0.6806385800838655 1106 1 0.10047930623333201 1106 2 0.1324936344653409 1106 3 -0.08980884538635081 1106 4 0.19684381910836635 1106 5 0.262865021793111 1106 6 -0.011837535542767341 1106 7 0.15749488501315057 1106 8 0.16723352881553666 1106 9 0.06885330819780933 1106 10-11 0.035639438891905684 1106 12-13 0.04189781629346356 1106 14-15 0.23636194386780573 1106 16-17 -0.023268921431309764 1106 18-19 -0.17667248004840275 1106 20-21 0.06931392060680963 1106 22-23 0.004935035538750299 1106 24-25 0.15305610231187927 1106 26-27 -0.046685923462362666 1106 28-29 -0.09496437822596704 1106 30-31 -0.19819734646220866 1106 32-33 -0.026908428932870265 1106 34-35 -0.10176068252463466 1106 36-37 -0.21800732575073312 1106 38-39 -0.1878478101171197 1106 40-41 -0.011036392360331604 1106 42-43 -0.5107073469722891 1106 44-45 -0.1359326776288583 1106 46-47 -0.33777068042600433 1106 48-49 -0.16140550024862677 1106 50-51 -0.4311171231827089 1106 52-53 -0.17753080510677677 1106 54-55 -0.17466493998193044 1106 56-57 -0.5075237195600337 1106 58-59 -0.907336849009468 1106 60-61 -0.37932537672200795 1106 62-63 -0.1703972862147083 1106 64-65 -0.13907607088015794 1106 66-67 -0.4639609516471275 1106 68-69 -0.6937024206557183 1106 70-71 -0.5184967572661705 1106 72-73 -0.20652112092386332 1106 74-75 -0.17315708947946362 1106 76-77 -0.15241551166612766 1106 78-79 0.17232962187056344 1106 80-81 0.2687814634426253 1106 82-83 0.1618918156811091 1106 84-85 0.04627464507492718 1106 86-87 0.06377314218592645 1106 88-89 0.003327066415941715 1106 90-91 0.11569538352003761 1106 92-93 -0.05989387911714772 1106 94-95 -0.22553314066993124 1106 96-97 -0.3467201722696096 1106 98-99 -0.21674069056037482 1106 100-101 -0.5068213698242943 1107 1 0.3338539976295891 1107 2 0.3297725936007794 1107 3 0.1941219154887861 1107 4 0.2689223980959383 1107 5 0.2891740111445813 1107 6 0.20872980916969652 1107 7 0.15340836208073938 1107 8 0.1409764867894836 1107 9 0.1803286055795965 1107 10-11 0.19334635740985817 1107 12-13 0.1686471258269293 1107 14-15 0.21735904613942836 1107 16-17 0.2695591509033406 1107 18-19 0.35740413293269313 1107 20-21 0.3502630382015681 1107 22-23 0.3824522331004232 1107 24-25 0.2931350622465061 1107 26-27 0.30148403220258757 1107 28-29 0.35922041173034813 1107 30-31 0.37990329282288826 1107 32-33 0.24428807780929418 1107 34-35 0.34095040842914415 1107 36-37 0.33531760277174527 1107 38-39 0.3261483311289908 1107 40-41 0.40825711069423676 1107 42-43 0.4645434154213106 1107 44-45 0.5480910556389418 1107 46-47 0.4929609576929437 1107 48-49 0.4138471935198922 1107 50-51 0.23403607813781946 1107 52-53 0.13191208646782826 1107 54-55 0.13203290173989757 1107 56-57 0.17142066416820256 1107 58-59 0.19391731260398615 1107 60-61 0.30107752806587484 1107 62-63 0.2944651765813475 1107 64-65 0.17139644332377202 1107 66-67 0.30079791619421314 1107 68-69 0.10267155613182055 1107 70-71 0.2586910076735123 1107 72-73 0.19041621794785257 1107 74-75 0.15905737838345857 1107 76-77 0.1432645021332135 1107 78-79 0.17333360383285168 1107 80-81 0.2353263868902502 1107 82-83 0.3598141224620335 1107 84-85 0.45395275946693303 1107 86-87 0.3837913104299915 1107 88-89 0.610498061693395 1107 90-91 0.6991334439933823 1107 92-93 0.6346371997022651 1107 94-95 0.6630994525472147 1107 96-97 0.8158643004084247 1107 98-99 0.8678039145433871 1107 100-101 0.36786886094837 1108 1 0.3334237905109845 1108 2 0.3037318406673677 1108 3 0.31219603237096294 1108 4 0.29481415875888217 1108 5 0.3280500289359338 1108 6 0.318819617324408 1108 7 0.3086415382089527 1108 8 0.3000183181431382 1108 9 0.2521151454501833 1108 10-11 0.29668636422093897 1108 12-13 0.37274202680834634 1108 14-15 0.3510196249776314 1108 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-0.6952339759173469 2316 56-57 -0.5748873185204033 2316 58-59 -0.5474455784753047 2316 60-61 -0.5769114521251595 2316 62-63 -0.6039619268103849 2316 64-65 -0.5902115336452098 2316 66-67 -0.5020089892985311 2316 68-69 -0.585352027784598 2316 70-71 -0.8641117034282786 2316 72-73 -0.4646251539992967 2316 74-75 -0.36485160333281286 2316 76-77 -0.3454549693735274 2316 78-79 -0.3999195276613037 2316 80-81 0.010439349464842707 2316 82-83 0.20274514379622843 2316 84-85 0.0021353489313860052 2316 86-87 -0.1551097591397017 2316 88-89 -0.225205261408167 2316 90-91 -0.3049065171131744 2316 92-93 -0.3227296760484357 2316 94-95 -0.4103672115773982 2316 96-97 -0.33451081160087526 2316 98-99 -0.5509345270497192 2316 100-101 -0.3899808463975347 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3314.0 3 797.0 4 737.0 5 777.0 6 985.0 7 1163.0 8 1413.0 9 1836.0 10 2405.0 11 3175.0 12 4320.0 13 5469.0 14 6692.0 15 8702.0 16 10901.0 17 13876.0 18 12768.0 19 10932.0 20 11159.0 21 12281.0 22 13524.0 23 15199.0 24 17007.0 25 19806.0 26 23479.0 27 27428.0 28 33006.0 29 39705.0 30 48709.0 31 61228.0 32 79016.0 33 103780.0 34 139038.0 35 188494.0 36 236090.0 37 236091.0 38 109697.0 39 6382.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.26094692318549 17.75489256015432 14.053888568972955 24.93027194768723 2 15.744496366211727 18.906554056737402 39.603063237182454 25.745886339868417 3 19.400909550010358 22.37154144965407 29.010157811096615 29.217391189238956 4 13.239460299557045 15.21236937072898 34.78081288672486 36.76735744298911 5 14.695143101498932 36.0956287119144 33.397167890401384 15.812060296185285 6 34.644164383271445 34.07271424911439 16.244952973864365 15.038168393749798 7 30.11847646454211 31.663945390033838 19.36717246893145 18.850405676492603 8 27.48801238967924 34.007816352126326 18.809743635563407 19.694427622631018 9 26.53657852115962 16.14675753686776 19.05719408580337 38.25946985616925 10-11 25.760644455431585 25.42811794895215 26.393894326200297 22.41734326941597 12-13 26.474401698059637 22.633018311590234 27.02311402269242 23.869465967657707 14-15 23.0976433650481 24.164310561176677 25.051953449709057 27.686092624066163 16-17 23.06235532957969 27.327508185925996 25.87358183692345 23.73655464757086 18-19 23.19477174883188 25.635718793528355 28.055287405886087 23.11422205175368 20-21 23.265278024163123 24.95651114823807 28.551755043250555 23.22645578434825 22-23 23.062997528100524 24.733027244508886 28.625615285863642 23.57835994152695 24-25 22.92533726582555 25.040971736039737 28.59808969591044 23.435601302224274 26-27 22.479225710704597 25.401311907084224 28.37704867573111 23.74241370648007 28-29 22.6366051678102 25.9316169958711 28.203140823423666 23.228637012895035 30-31 22.618499937446092 25.463279532560136 28.111792106557438 23.806428423436333 32-33 22.27572021227832 25.401215619368493 28.242117752576945 24.080946415776243 34-35 23.133407843564257 25.55507860476338 28.097152780282965 23.214360771389394 36-37 23.25662747974077 25.126969743704137 28.026142562217327 23.590260214337768 38-39 22.6870165860115 25.44977457914547 28.040104231294205 23.823104603548828 40-41 22.99496223746143 25.285167139560084 28.238205927849975 23.481664695128508 42-43 22.843129696607935 25.394596959031666 27.987589423944527 23.774683920415875 44-45 22.627483908192 25.736339166613732 28.10359498401406 23.532581941180208 46-47 23.10916890711984 25.99361313687487 27.481212286440094 23.416005669565195 48-49 22.939616202969546 26.16775677481065 27.206505379848288 23.68612164237152 50-51 23.00718068704856 26.36113456443429 27.210846992872252 23.4208377556449 52-53 23.394194006879 25.790430010335708 27.35193539721002 23.463440585575274 54-55 22.82758837454363 26.307979639405065 27.447597274446196 23.41683471160511 56-57 22.840758481369804 26.335835181101093 27.306566672158688 23.51683966537042 58-59 22.821556445620217 25.871744667166475 27.28726068667614 24.01943820053717 60-61 22.42505230755386 26.117250971709588 27.62070439515793 23.836992325578628 62-63 22.712382190015166 26.157422723795964 27.742282966284048 23.387912119904826 64-65 22.519825712385735 26.335626069033513 27.44321922589567 23.70132899268508 66-67 22.385981372722867 26.335510662117922 27.46749225186789 23.811015713291322 68-69 22.96543058259301 25.916012805056383 27.554030429874583 23.564526182476023 70-71 22.792894114174747 26.08288523661525 27.155107806263395 23.969112842946608 72-73 22.624991934936862 26.38441978694998 27.144074287916965 23.84651399019619 74-75 22.57543226457103 26.677301018667688 27.212790604182736 23.534476112578545 76-77 22.846874042586418 26.293033569277537 27.097641462302636 23.76245092583341 78-79 22.879659686868514 26.56711312142943 27.099894528866937 23.453332662835123 80-81 23.03517063910344 26.473427650171477 26.900943499224834 23.59045821150025 82-83 22.795645349975917 26.548232519731307 26.98530540513358 23.6708167251592 84-85 22.768427394261888 26.678759875909513 26.93343849551037 23.619374234318226 86-87 23.035079007085677 26.64279520422474 26.597171883462355 23.724953905227228 88-89 22.902672987764262 26.728686397871115 26.41121967248049 23.957420941884134 90-91 22.71162614308661 26.683912104523632 26.614650172641902 23.98981157974785 92-93 23.01886068255282 26.362701768538482 26.684618192033454 23.933819356875244 94-95 22.833483841570253 26.12201987899998 26.96904648371616 24.07544979571361 96-97 23.159596702992694 25.808963391147007 26.842289532518038 24.189150373342265 98-99 22.874325875431342 26.112142166787493 26.539472373755608 24.47405958402556 100-101 23.379734271750998 25.57545807307113 26.717018523614932 24.327789131562938 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 598.0 1 429.5 2 379.5 3 495.0 4 414.5 5 405.5 6 751.0 7 1598.0 8 2475.5 9 2700.5 10 2165.5 11 1641.0 12 1541.0 13 1561.5 14 1617.5 15 1674.5 16 1777.0 17 1959.5 18 2136.5 19 2296.5 20 2507.5 21 2884.0 22 3415.5 23 4201.0 24 5088.5 25 6181.0 26 7634.0 27 9431.5 28 11016.5 29 12292.0 30 13798.0 31 16479.0 32 21059.5 33 22448.0 34 21509.0 35 22707.5 36 24858.0 37 27529.0 38 30471.5 39 32692.5 40 34835.0 41 38079.0 42 40332.5 43 43007.5 44 45880.5 45 49265.5 46 52013.5 47 53935.0 48 55805.0 49 56227.0 50 55848.5 51 54823.5 52 55241.0 53 57378.5 54 59265.5 55 57180.0 56 53430.5 57 50258.5 58 48232.0 59 44470.0 60 36680.5 61 30572.0 62 25920.0 63 19894.0 64 14963.0 65 11972.0 66 9178.5 67 6946.0 68 5387.0 69 4168.0 70 3308.0 71 2481.0 72 1871.0 73 1532.5 74 1217.5 75 965.5 76 732.5 77 495.5 78 352.0 79 251.0 80 154.0 81 92.0 82 63.5 83 38.5 84 22.5 85 22.5 86 21.0 87 13.0 88 8.5 89 7.0 90 5.0 91 3.0 92 2.5 93 1.5 94 1.0 95 2.0 96 2.5 97 1.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1524433614025848 2 0.037118370549848116 3 0.03467027837454619 4 0.04082359113949428 5 0.04664608063750967 6 0.05544597953791929 7 0.029641764717169262 8 0.02950943541039619 9 0.033611643920361575 10-11 0.03467027837454619 12-13 0.0317590336255385 14-15 0.027326001848640414 16-17 0.026697437641468303 18-19 0.033909384860601 20-21 0.04294086004786351 22-23 0.039665709705229854 24-25 0.05878729453393949 26-27 0.044958881976152935 28-29 0.03751535847016735 30-31 0.045256622916392354 32-33 0.04158448465343947 34-35 0.041452155346666394 36-37 0.03890481619128466 38-39 0.021073442103612523 40-41 0.01941932576894906 42-43 0.016574245673327905 44-45 0.0035067266294865427 46-47 0.010189356621526934 48-49 0.009229969147422126 50-51 0.0069472886055865464 52-53 0.007608935139451932 54-55 0.008502157960170202 56-57 0.006484136031880776 58-59 0.00860140494025001 60-61 0.008402910980090394 62-63 0.003242068015940388 64-65 0.005690160191242314 66-67 0.003242068015940388 68-69 0.006814959298813469 70-71 0.01769904478089906 72-73 0.015449446565756749 74-75 0.015912599139462517 76-77 0.009792368701207704 78-79 0.004433031776898082 80-81 0.006087148111561545 82-83 0.005094678310763467 84-85 0.004267620143431736 86-87 0.006120230438254815 88-89 0.023587698932300987 90-91 0.029774094023942342 92-93 0.018162197354604826 94-95 0.02577113249405676 96-97 0.028781624223144262 98-99 0.016838904286874058 100-101 0.10622735101208762 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1511381.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.75202887348695 #Duplication Level Percentage of deduplicated Percentage of total 1 76.70932387702409 41.999911157804846 2 12.91095849910999 14.138023450553236 3 4.332378826485302 7.116195917958203 4 1.9987471808553976 4.3774185342798155 5 1.0951635683187744 2.9981213656890264 6 0.6814033802260467 2.2384930529176867 7 0.43729761810680057 1.6760052269023429 8 0.2953604324039938 1.2937266338455247 9 0.23307872421860532 1.1485379734391323 >10 1.1780941571980938 11.484637843448377 >50 0.07672769301679742 2.9307658911851564 >100 0.044684783995353194 4.3676049208308845 >500 0.004601568634793623 1.7761474908532873 >1k 0.002058596494512937 2.046391888655296 >5k 1.2109391144193747E-4 0.4080186516372814 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6154 0.40717727694075817 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4640 0.3070039917135388 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3454 0.2285327127971041 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2681 0.17738743572930982 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2114 0.13987207725914247 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2032 0.1344465756814463 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2021 0.1337187644941944 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1699 0.11241374610372898 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 6.616465338653853E-5 0.0 0.0 0.0 0.0 6 1.3232930677307706E-4 0.0 0.0 0.0 0.0 7 1.3232930677307706E-4 0.0 0.0 0.0 0.0 8 1.3232930677307706E-4 0.0 0.0 0.0 0.0 9 1.3232930677307706E-4 0.0 0.0 0.0 0.0 10-11 1.3232930677307706E-4 0.0 0.0 0.0 0.0 12-13 1.3232930677307706E-4 0.0 0.0 0.0 0.0 14-15 1.3232930677307706E-4 0.0 0.0 0.0 0.0 16-17 1.3232930677307706E-4 0.0 0.0 0.0 3.3082326693269265E-5 18-19 1.3232930677307706E-4 0.0 0.0 0.0 1.984939601596156E-4 20-21 1.3232930677307706E-4 0.0 0.0 6.616465338653853E-5 2.3157628685288486E-4 22-23 1.3232930677307706E-4 0.0 0.0 1.3232930677307706E-4 2.646586135461541E-4 24-25 1.3232930677307706E-4 0.0 0.0 1.984939601596156E-4 2.646586135461541E-4 26-27 1.3232930677307706E-4 0.0 0.0 4.962349003990391E-4 3.308232669326927E-4 28-29 2.3157628685288486E-4 0.0 0.0 0.0014225400478105783 3.308232669326927E-4 30-31 2.646586135461541E-4 0.0 0.0 0.005888654151401929 3.308232669326927E-4 32-33 2.646586135461541E-4 0.0 0.0 0.014688553051811555 3.308232669326927E-4 34-35 2.646586135461541E-4 0.0 0.0 0.02811997768927888 3.308232669326927E-4 36-37 3.6390559362596195E-4 0.0 0.0 0.05015280726699621 3.308232669326927E-4 38-39 4.631525737057698E-4 0.0 0.0 0.0856832261355674 3.308232669326927E-4 40-41 4.631525737057698E-4 0.0 0.0 0.12409180742645302 3.308232669326927E-4 42-43 5.293172270923082E-4 0.0 0.0 0.16375751713168288 3.308232669326927E-4 44-45 5.293172270923082E-4 0.0 0.0 0.21639149890067427 3.308232669326927E-4 46-47 5.623995537855776E-4 0.0 0.0 0.2823576583270532 3.308232669326927E-4 48-49 5.954818804788469E-4 0.0 0.0 0.35130122715582635 3.969879203192312E-4 50-51 5.954818804788469E-4 0.0 0.0 0.43245217453441587 3.969879203192312E-4 52-53 5.954818804788469E-4 0.0 0.0 0.5163489550285467 3.969879203192312E-4 54-55 5.954818804788469E-4 0.0 0.0 0.6079539176422093 3.969879203192312E-4 56-57 6.616465338653854E-4 0.0 0.0 0.7205992400327912 3.969879203192312E-4 58-59 6.616465338653854E-4 0.0 0.0 0.8403241803357326 3.969879203192312E-4 60-61 6.616465338653854E-4 6.616465338653853E-5 0.0 0.9705031358737473 4.631525737057698E-4 62-63 6.616465338653854E-4 6.616465338653853E-5 0.0 1.09995428022451 4.631525737057698E-4 64-65 6.616465338653854E-4 6.616465338653853E-5 0.0 1.2446232948541764 4.631525737057698E-4 66-67 6.616465338653854E-4 6.616465338653853E-5 0.0 1.4157581708384583 4.631525737057698E-4 68-69 6.616465338653854E-4 6.616465338653853E-5 0.0 1.6074702540259538 4.631525737057698E-4 70-71 6.616465338653854E-4 6.616465338653853E-5 0.0 1.8142017135321935 4.631525737057698E-4 72-73 6.616465338653854E-4 6.616465338653853E-5 0.0 2.0254323694687177 4.96234900399039E-4 74-75 6.616465338653854E-4 6.616465338653853E-5 0.0 2.2415922920825393 5.293172270923082E-4 76-77 6.616465338653854E-4 6.616465338653853E-5 0.0 2.4726392617083315 5.293172270923082E-4 78-79 6.616465338653854E-4 6.616465338653853E-5 0.0 2.741168507477598 5.293172270923082E-4 80-81 6.616465338653854E-4 6.616465338653853E-5 0.0 3.031598253517809 5.293172270923082E-4 82-83 6.616465338653854E-4 6.616465338653853E-5 0.0 3.3355586711755674 5.293172270923082E-4 84-85 6.947288605586546E-4 6.616465338653853E-5 0.0 3.6485505640205877 5.293172270923082E-4 86-87 7.278111872519239E-4 6.616465338653853E-5 0.0 3.9773888913516844 5.623995537855776E-4 88-89 7.278111872519239E-4 6.616465338653853E-5 0.0 4.337258441121067 5.954818804788469E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2375 0.0 54.102104 1 GTATCAA 4425 0.0 45.38509 1 TATCAAC 5805 0.0 34.636494 2 ATCAACG 5860 0.0 34.066933 3 TCAACGC 5920 0.0 33.882244 4 CAACGCA 6070 0.0 32.964466 5 GTGGTAT 1180 0.0 32.26428 1 AACGCAG 6335 0.0 31.656355 6 TGGTATC 1130 0.0 31.548492 2 ACGCAGA 7290 0.0 27.244967 7 CGCAGAG 7385 0.0 26.765808 8 TACATGG 5520 0.0 26.69429 2 GTACATG 5630 0.0 26.373041 1 TAAGGTG 655 0.0 24.671198 5 ACATGGG 5660 0.0 24.437567 3 GCAGAGT 8325 0.0 23.340208 9 ACCGAGT 410 0.0 23.178413 8 GAGTACT 5185 0.0 21.52988 12-13 CATGGGG 3860 0.0 21.426111 4 GGACCGA 445 0.0 21.358221 6 >>END_MODULE