FastQCFastQC Report
Thu 26 May 2016
SRR938306_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938306_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511381
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65720.4348341020563313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55170.3650303927335331No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42570.2816629294664945No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC18660.12346324321928091No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA18620.12319858460573475No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16490.10910551343440206No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG16290.10778222036667129No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA16020.10599577472523473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28900.058.2683831
GTATCAA51550.042.8170621
ATCAACG70250.031.4018063
TATCAAC70450.031.127732
TCAACGC71250.030.8933284
CAACGCA72650.030.363445
GTGGTAT12350.029.6586461
TGGTATC11800.029.4285162
AACGCAG76400.029.0940516
AGGTAAG8450.027.5846142
GTAAGGT9050.026.265764
TAAGGTG9500.026.0224575
ACGCAGA87800.025.2082737
CGCAGAG89150.024.773278
AAGGTAA9050.023.6532841
GGTAAGG10450.022.7476583
GCAGAGT96450.022.4550659
AGAGTAC85450.021.70504610-11
GTACATG54250.021.1322061
GAGTACT62200.021.03695712-13
Produced by FastQC (version 0.11.5)