##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938306_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1511381 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.121225554641747 31.0 31.0 33.0 30.0 34.0 2 31.38644855268129 31.0 31.0 34.0 30.0 34.0 3 31.41527715380834 31.0 31.0 34.0 28.0 34.0 4 35.19968360062751 37.0 35.0 37.0 32.0 37.0 5 35.0382213353218 35.0 35.0 37.0 32.0 37.0 6 35.120104063766846 37.0 35.0 37.0 32.0 37.0 7 35.04565758071592 37.0 35.0 37.0 32.0 37.0 8 35.030313335949046 37.0 35.0 37.0 32.0 37.0 9 36.485115268750896 38.0 35.0 39.0 32.0 39.0 10-11 36.3331572250809 38.0 35.0 39.0 32.0 39.0 12-13 36.190335196750524 38.0 35.0 39.0 32.0 39.0 14-15 36.96211842017334 38.0 36.0 40.0 31.0 41.0 16-17 36.80839841178366 38.0 36.0 40.0 31.0 41.0 18-19 36.92729133157027 38.5 36.0 40.0 31.0 41.0 20-21 36.98388559866771 39.0 36.0 40.0 31.0 41.0 22-23 36.8202174038181 39.0 36.0 40.0 31.0 41.0 24-25 36.63426826194057 38.0 35.0 40.0 30.0 41.0 26-27 36.30388333583656 38.0 35.0 40.0 30.0 41.0 28-29 36.10542708952938 38.0 34.5 40.0 29.5 41.0 30-31 35.738168271269785 38.0 34.0 40.0 28.5 41.0 32-33 35.39962722834282 38.0 34.0 40.0 27.0 41.0 34-35 35.12537804828828 38.0 34.0 40.0 27.0 41.0 36-37 35.04329219435735 38.0 33.5 40.0 26.5 41.0 38-39 34.80259213262572 38.0 33.0 40.0 26.0 41.0 40-41 34.494160307692106 37.0 33.0 40.0 25.0 41.0 42-43 34.13796984347428 37.0 33.0 40.0 24.0 41.0 44-45 33.69699863899308 37.0 32.0 39.5 23.5 40.5 46-47 33.113916676205406 36.5 31.0 39.0 22.0 40.0 48-49 32.53179608583144 36.0 31.0 39.0 18.5 40.0 50-51 31.91749135393392 35.5 30.0 39.0 16.0 40.0 52-53 31.14273932251365 35.0 29.0 38.0 11.5 40.0 54-55 30.555512475014574 34.0 28.0 38.0 8.5 40.0 56-57 29.98094325653161 34.0 27.5 38.0 7.5 40.0 58-59 29.261298441623918 33.5 26.0 37.5 4.0 39.5 60-61 28.632466267605587 33.0 26.0 37.0 2.0 39.0 62-63 27.930064623016964 32.5 24.5 36.5 2.0 39.0 64-65 27.13189956734933 32.0 22.5 36.0 2.0 39.0 66-67 26.383420196495788 31.0 21.5 35.0 2.0 38.0 68-69 25.58288710788345 31.0 19.5 35.0 2.0 38.0 70-71 24.743600058489555 30.0 17.5 34.0 2.0 37.0 72-73 24.053846779865566 29.0 13.5 34.0 2.0 36.0 74-75 23.304592951744134 29.0 9.0 34.0 2.0 36.0 76-77 22.535009041399885 28.0 8.0 32.5 2.0 35.0 78-79 23.039715002372006 29.0 7.0 34.0 2.0 35.0 80-81 22.54897441479018 29.0 4.0 34.0 2.0 35.0 82-83 21.791264082319415 29.0 2.0 33.5 2.0 35.0 84-85 20.9444537810122 27.0 2.0 33.0 2.0 35.0 86-87 20.01967141309835 26.0 2.0 32.5 2.0 34.5 88-89 18.992732143648755 25.0 2.0 32.0 2.0 34.0 90-91 18.06982819024455 23.5 2.0 32.0 2.0 34.0 92-93 16.951652495300657 19.5 2.0 31.0 2.0 34.0 94-95 15.776166962532942 11.5 2.0 31.0 2.0 34.0 96-97 14.428802201430347 2.0 2.0 30.5 2.0 34.0 98-99 12.964495054522983 2.0 2.0 29.5 2.0 34.0 100-101 10.509422839112045 2.0 2.0 24.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33571429427513877 1101 2 0.44036574920089677 1101 3 0.42058807118668895 1101 4 0.3356139784470358 1101 5 0.16761048822186808 1101 6 0.24119616713657166 1101 7 0.32646203778796234 1101 8 0.43662833628996367 1101 9 0.33950965427579405 1101 10-11 0.41059629647664053 1101 12-13 0.47656253043799524 1101 14-15 0.5793151854295076 1101 16-17 0.6073256671670748 1101 18-19 0.5705369356942711 1101 20-21 0.5455587655972636 1101 22-23 0.5449121090203448 1101 24-25 0.7803385257476094 1101 26-27 0.5631326691963778 1101 28-29 0.5838637005654022 1101 30-31 0.630304945272627 1101 32-33 0.6022236016069584 1101 34-35 0.4502301976805754 1101 36-37 0.7591122815791707 1101 38-39 0.755684268166128 1101 40-41 0.9453321880138148 1101 42-43 1.1095621282854466 1101 44-45 0.8668921408419834 1101 46-47 1.041588942733732 1101 48-49 0.9760869093977576 1101 50-51 0.9644394177314659 1101 52-53 1.097149054619564 1101 54-55 0.7614301952319451 1101 56-57 0.7386824753532011 1101 58-59 0.7137083649343303 1101 60-61 0.7606560106389892 1101 62-63 0.8572417276349711 1101 64-65 0.7806682832332328 1101 66-67 0.9688578791489135 1101 68-69 0.8463710467064622 1101 70-71 0.712779651593678 1101 72-73 0.47809052101572647 1101 74-75 0.5736148640775021 1101 76-77 0.41745487903563117 1101 78-79 0.5715914453727393 1101 80-81 0.6882486455610852 1101 82-83 0.5762341663514725 1101 84-85 0.597805715315392 1101 86-87 0.5647215163775847 1101 88-89 0.6079957161120788 1101 90-91 1.167878391123434 1101 92-93 0.8604549618663171 1101 94-95 0.9088208827873601 1101 96-97 0.8896000641733863 1101 98-99 0.7226512122817379 1101 100-101 0.20782482683000048 1102 1 0.3442146308146441 1102 2 0.4471413207752484 1102 3 0.4804744619633361 1102 4 0.3914876784274952 1102 5 0.325824766181789 1102 6 0.35634695854721343 1102 7 0.49432468624530657 1102 8 0.4401497879694034 1102 9 0.3476870214512502 1102 10-11 0.45712220459049746 1102 12-13 0.3906292711071657 1102 14-15 0.5399688998485956 1102 16-17 0.5772864630287273 1102 18-19 0.508861321781076 1102 20-21 0.5211908627811894 1102 22-23 0.5896864511398974 1102 24-25 0.6167670952375772 1102 26-27 0.6146262668036897 1102 28-29 0.520707584231296 1102 30-31 0.5423985873900037 1102 32-33 0.518136899259865 1102 34-35 0.521867991609831 1102 36-37 0.5922975872873124 1102 38-39 0.49765583329195096 1102 40-41 0.9424665134149492 1102 42-43 0.7543005773811302 1102 44-45 0.7676011128766334 1102 46-47 0.5836648622465077 1102 48-49 1.047559826109655 1102 50-51 0.9783566154429941 1102 52-53 0.852697885416184 1102 54-55 0.8644214614889734 1102 56-57 0.4969843485755092 1102 58-59 0.704724931362886 1102 60-61 0.5687514820114217 1102 62-63 0.48806908558272966 1102 64-65 0.8162841369579681 1102 66-67 0.6077995031148689 1102 68-69 0.536501618606497 1102 70-71 0.27480401813948063 1102 72-73 0.29134162833925714 1102 74-75 0.347643852613448 1102 76-77 0.598636960478192 1102 78-79 0.8529479709823136 1102 80-81 0.9973740906617223 1102 82-83 0.8814161283767881 1102 84-85 0.7804594785869874 1102 86-87 0.552505335883847 1102 88-89 0.6098887508299313 1102 90-91 0.47257623835620777 1102 92-93 0.38446766623264494 1102 94-95 0.3124437034226588 1102 96-97 0.15163508328014608 1102 98-99 0.11756878724319897 1102 100-101 -0.1572543820850374 1103 1 0.35049918274378555 1103 2 0.39543892150501847 1103 3 0.2942315361800283 1103 4 0.3303328936488228 1103 5 0.33591216011768665 1103 6 0.21226033527170074 1103 7 0.35334560679126525 1103 8 0.2956588301224272 1103 9 0.45292672566445447 1103 10-11 0.44558818412867396 1103 12-13 0.466839129268962 1103 14-15 0.5710302486091194 1103 16-17 0.47341081458617396 1103 18-19 0.4680782495723008 1103 20-21 0.38622634199301586 1103 22-23 0.42415698910802746 1103 24-25 0.556788943721557 1103 26-27 0.5121612020970545 1103 28-29 0.6132199239719469 1103 30-31 0.7087858678238774 1103 32-33 0.7814111311552452 1103 34-35 0.6473555522600165 1103 36-37 0.6551634216058133 1103 38-39 0.6262070310921573 1103 40-41 0.7348859282897422 1103 42-43 0.9423846344100042 1103 44-45 0.9705629782941685 1103 46-47 0.9107135344301227 1103 48-49 1.015315618513668 1103 50-51 1.0008355205809956 1103 52-53 1.1084929107247774 1103 54-55 1.0449876478472362 1103 56-57 1.2570917298767732 1103 58-59 1.0345973923121612 1103 60-61 1.183426860984973 1103 62-63 1.0213549298097249 1103 64-65 0.6719393162413567 1103 66-67 1.0178576696246928 1103 68-69 0.7112400134834438 1103 70-71 0.5712635021115808 1103 72-73 0.754677264042332 1103 74-75 0.8993652562638594 1103 76-77 0.9617067969880928 1103 78-79 0.9681482640429593 1103 80-81 0.937036494230874 1103 82-83 0.7757999408389473 1103 84-85 0.62815247789759 1103 86-87 0.7824796340979638 1103 88-89 0.8588418716112827 1103 90-91 0.5203961738199574 1103 92-93 0.5624839890292499 1103 94-95 0.5496413044145569 1103 96-97 0.6439255304990841 1103 98-99 0.49214905257055896 1103 100-101 0.08440498864716695 1104 1 0.35497331863960113 1104 2 0.36332238468527933 1104 3 0.3533658049216939 1104 4 0.27153042049555154 1104 5 0.30486406889941975 1104 6 0.10988703971202085 1104 7 0.16157381834923967 1104 8 0.21820370228541464 1104 9 0.35436748420021047 1104 10-11 0.4781796033843122 1104 12-13 0.395352334095314 1104 14-15 0.5032292557746274 1104 16-17 0.5799610656660974 1104 18-19 0.5634393325020497 1104 20-21 0.5073598067684486 1104 22-23 0.5241876163931067 1104 24-25 0.48064456797581556 1104 26-27 0.41233411864408254 1104 28-29 0.5624202647004921 1104 30-31 0.6388746231437779 1104 32-33 0.659610565826604 1104 34-35 0.6302165554312182 1104 36-37 0.7978980553226265 1104 38-39 0.6184179701374788 1104 40-41 0.6156834181285049 1104 42-43 0.6454755974466906 1104 44-45 0.6089751768865881 1104 46-47 0.7631227213395064 1104 48-49 0.630342927842868 1104 50-51 0.9070129801753062 1104 52-53 0.9818069743912474 1104 54-55 0.8927746669829091 1104 56-57 0.8410454252501403 1104 58-59 0.8366431992454118 1104 60-61 0.6676431215210812 1104 62-63 0.6383693437757678 1104 64-65 0.7662509361057062 1104 66-67 0.6134926434319858 1104 68-69 0.3893652726987433 1104 70-71 0.3841689148461498 1104 72-73 0.5261538501209522 1104 74-75 0.4468088350621251 1104 76-77 0.5625610111111037 1104 78-79 0.3340002768298511 1104 80-81 0.6472408037451132 1104 82-83 0.48576672937668164 1104 84-85 0.44246690526306764 1104 86-87 0.47433038350934353 1104 88-89 0.45761981103306937 1104 90-91 0.4600388873974133 1104 92-93 0.7301739579553157 1104 94-95 0.6259635417377325 1104 96-97 0.6970043702354349 1104 98-99 0.5451087330258844 1104 100-101 0.26534540581631383 1105 1 0.26660186327213964 1105 2 0.3073760016826874 1105 3 0.26552715444481834 1105 4 0.27489924299674584 1105 5 0.2780188035407747 1105 6 0.2964357415064143 1105 7 0.3675926441220412 1105 8 0.38133235752949446 1105 9 0.3263687429907165 1105 10-11 0.36655168191538934 1105 12-13 0.4265479046483378 1105 14-15 0.5509880478342808 1105 16-17 0.43637871615231916 1105 18-19 0.45896314825750295 1105 20-21 0.4234012845386488 1105 22-23 0.44467396443621965 1105 24-25 0.47898134428811545 1105 26-27 0.40517381292001176 1105 28-29 0.4335768252112402 1105 30-31 0.46625113548397223 1105 32-33 0.5897156000538715 1105 34-35 0.5145564537932401 1105 36-37 0.5925615937079485 1105 38-39 0.5792018164902615 1105 40-41 0.6458192989546987 1105 42-43 0.8067210522470845 1105 44-45 0.8351781163493541 1105 46-47 0.3788029200040697 1105 48-49 0.27063949140711685 1105 50-51 0.1733379973902025 1105 52-53 0.1842256281708856 1105 54-55 0.14931991021924418 1105 56-57 0.3436631798046861 1105 58-59 0.5673612706786564 1105 60-61 0.41352338686414924 1105 62-63 0.39287061035581416 1105 64-65 0.3009532041096783 1105 66-67 0.12960547343606876 1105 68-69 0.23466739799825476 1105 70-71 0.05749377315432369 1105 72-73 0.44339460626768457 1105 74-75 0.6277194640523192 1105 76-77 0.4030504438141058 1105 78-79 0.5005966817989602 1105 80-81 0.754784510883141 1105 82-83 0.831430912736348 1105 84-85 0.7647918975514223 1105 86-87 0.6713130252868531 1105 88-89 0.731341624563818 1105 90-91 0.9087494060876473 1105 92-93 0.4316435335497353 1105 94-95 0.32249002774926794 1105 96-97 0.2917204101552944 1105 98-99 0.161808583155036 1105 100-101 0.05196670617182875 1106 1 0.20666618050903907 1106 2 0.20928464170176042 1106 3 0.15760380562819165 1106 4 0.07785707193765745 1106 5 0.06479063277541286 1106 6 -0.04340340612883864 1106 7 0.15567604183188166 1106 8 0.08966560853992434 1106 9 -0.16880021982633764 1106 10-11 0.04708119599293781 1106 12-13 -0.08527366408370796 1106 14-15 0.020738497481964657 1106 16-17 0.219937482520578 1106 18-19 0.2796539888797085 1106 20-21 0.2755618655671057 1106 22-23 0.3313421656017752 1106 24-25 0.18746178864738283 1106 26-27 -0.025479514802192682 1106 28-29 0.14132031495149988 1106 30-31 0.45167668890790935 1106 32-33 0.22815045069707196 1106 34-35 0.0744648053528536 1106 36-37 -0.5644594631732289 1106 38-39 -0.16399666534370994 1106 40-41 0.15980628930184082 1106 42-43 0.30281697094103066 1106 44-45 0.19125249278630463 1106 46-47 -0.07052157119100144 1106 48-49 -0.03360520913839338 1106 50-51 0.29311633803710535 1106 52-53 0.20883803548353086 1106 54-55 -0.21597249085003156 1106 56-57 0.11055649788523425 1106 58-59 0.1517005898748387 1106 60-61 -0.3519816377964986 1106 62-63 -0.13593746598188616 1106 64-65 -0.386383261445431 1106 66-67 -0.2863207810497066 1106 68-69 -0.7995680626247257 1106 70-71 -0.685661077541571 1106 72-73 -0.5212612082647468 1106 74-75 -0.2519488207533094 1106 76-77 -0.22305177752122418 1106 78-79 -0.453373784695291 1106 80-81 -0.6206876109600223 1106 82-83 -0.16472757136315153 1106 84-85 -0.16551106389973214 1106 86-87 -0.1563923851718414 1106 88-89 -0.37382687065914766 1106 90-91 -0.5896275155758524 1106 92-93 -0.5154315666701663 1106 94-95 -0.4965961748664931 1106 96-97 -0.6931329990995128 1106 98-99 -1.0466764712867462 1106 100-101 -0.7724388730817466 1107 1 0.18690388614219344 1107 2 0.13731828225690634 1107 3 0.08640799817694855 1107 4 0.137222209021985 1107 5 0.16836127880700502 1107 6 0.28277351713763466 1107 7 0.12523424314908027 1107 8 0.24995341004253646 1107 9 0.18033409131550115 1107 10-11 0.15984218967867037 1107 12-13 0.32616796743526066 1107 14-15 0.3828869456222179 1107 16-17 0.23660325009892347 1107 18-19 0.2283150465672108 1107 20-21 0.29234734509086735 1107 22-23 0.20930307990134622 1107 24-25 0.1865125844737534 1107 26-27 0.13436923190198513 1107 28-29 0.35960480780907744 1107 30-31 0.24003146383280693 1107 32-33 0.282834501069992 1107 34-35 0.3473662499546961 1107 36-37 0.3084485747429966 1107 38-39 0.1868645613723956 1107 40-41 0.3501824145774819 1107 42-43 0.19505873778945215 1107 44-45 0.3682773229418643 1107 46-47 0.10997090943364896 1107 48-49 0.0939029975400345 1107 50-51 0.13127244774186053 1107 52-53 0.08857598120656718 1107 54-55 -0.15779548233666318 1107 56-57 -0.140219049043516 1107 58-59 -0.6174459954876035 1107 60-61 -0.4686876312524717 1107 62-63 -0.4212548208507165 1107 64-65 -0.2375460157459024 1107 66-67 -0.049884360913058146 1107 68-69 0.12351026928005382 1107 70-71 -0.0799855658437636 1107 72-73 -0.045875749120316556 1107 74-75 -0.21475736622153718 1107 76-77 -0.17973370204975225 1107 78-79 -0.11359781752599929 1107 80-81 0.15647567879749857 1107 82-83 0.19633048243696294 1107 84-85 0.24515002575536116 1107 86-87 0.29176901250425047 1107 88-89 -0.20652584723023892 1107 90-91 -0.11301080492046012 1107 92-93 -0.06132453120412862 1107 94-95 -0.18293938951350874 1107 96-97 -0.5485436127372836 1107 98-99 -0.5742035588199865 1107 100-101 -0.912811368704519 1108 1 0.25668322278474776 1108 2 0.20291551551559195 1108 3 0.17293574298866332 1108 4 0.2667958202334333 1108 5 0.3647608257673056 1108 6 0.21146620348761758 1108 7 0.23389669245300837 1108 8 0.23970519278987723 1108 9 0.36389514847693505 1108 10-11 0.31773884451195755 1108 12-13 0.2697050568357824 1108 14-15 0.5320453005411565 1108 16-17 0.4737112262807983 1108 18-19 0.3931092050382219 1108 20-21 0.445885492272005 1108 22-23 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-0.6073167122843692 2316 50-51 -0.8539837914146808 2316 52-53 -0.6959953329723803 2316 54-55 -0.5667657593571178 2316 56-57 -0.5951388789800269 2316 58-59 -0.27700158070046754 2316 60-61 -0.03673066189058716 2316 62-63 -0.20087008080762558 2316 64-65 -0.04680513377987694 2316 66-67 -0.030697405468931294 2316 68-69 0.18094292070709983 2316 70-71 0.17177989144017758 2316 72-73 0.18741055619321045 2316 74-75 0.09933340421181569 2316 76-77 0.09815423145008495 2316 78-79 0.3024876717878371 2316 80-81 0.0029142595681221906 2316 82-83 0.1367744205887611 2316 84-85 0.06731023242444323 2316 86-87 0.41955542267755064 2316 88-89 0.5501327484114071 2316 90-91 0.6334864019964748 2316 92-93 0.7404027952529475 2316 94-95 0.6653347139354224 2316 96-97 1.0954293334410554 2316 98-99 1.1212541064294843 2316 100-101 1.0796358677638036 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 25.0 4 61.0 5 128.0 6 304.0 7 739.0 8 1737.0 9 2795.0 10 4402.0 11 6553.0 12 9196.0 13 13022.0 14 16671.0 15 21548.0 16 26760.0 17 31134.0 18 31107.0 19 30387.0 20 31316.0 21 33292.0 22 35792.0 23 38770.0 24 42720.0 25 48070.0 26 54857.0 27 62853.0 28 71916.0 29 80665.0 30 89885.0 31 98820.0 32 108071.0 33 117153.0 34 123084.0 35 119499.0 36 96367.0 37 51590.0 38 9942.0 39 142.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.66282357253589 20.919179895842777 15.830283669970735 21.587712861650594 2 18.57026554764237 22.16204230759048 36.073773529956846 23.193918614810304 3 21.78873709116509 24.092279312516872 29.841243165132514 24.27774043118552 4 16.177059259048512 17.835542460835487 33.64724050388354 32.340157776232466 5 14.654028498781651 37.30400466151075 33.242862061659075 14.799104778048521 6 34.21043403350975 35.76252447265117 15.44137447804359 14.585667015795487 7 29.52200669453963 32.610043397396154 19.347735613984828 18.520214294079388 8 27.604356545437582 35.24491838920828 18.206196849106878 18.94452821624726 9 26.69975340433683 16.27306417111238 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34-35 23.43258728269691 25.66839010352201 28.470621009651442 22.42840160412963 36-37 23.536773572452944 25.27677565664435 28.57681155105194 22.609639219850767 38-39 23.020428607726267 25.627368802735635 28.433142858655263 22.919059730882836 40-41 23.292794283067558 25.550420168067227 28.504962614967244 22.651822933897968 42-43 23.26275972176366 25.624711769465858 28.30336176877113 22.809166739999352 44-45 23.141484509862174 25.66950358645504 28.463107581741465 22.72590432194132 46-47 23.648801989703458 26.037147482997337 27.75987656322264 22.55417396407656 48-49 23.499402202356652 26.29578511308532 27.320212441469096 22.884600243088936 50-51 23.481901651535914 26.405122202806574 27.403116752162425 22.709859393495087 52-53 23.97479523693893 26.09031078199342 27.280050496863467 22.654843484204182 54-55 23.598583017783074 26.406445495874305 27.364939747158395 22.63003173918423 56-57 23.48511063722516 26.489912206121417 27.229335290042684 22.795641866610737 58-59 23.46549281749605 26.1705354242246 27.16049096819399 23.203480790085358 60-61 23.116904341129075 26.34587175569893 27.51132904277611 23.02589486039589 62-63 23.291182038149216 26.431786558121345 27.543352735015198 22.73367866871424 64-65 23.09278732496968 26.43628575455163 27.343370070154382 23.127556850324307 66-67 22.934462896669633 26.539345982034305 27.282375285376514 23.243815835919545 68-69 23.574594812582106 26.110078382604907 27.316336154948313 22.998990649864677 70-71 23.317744920367424 26.33406313042592 26.958989108621996 23.38920284058466 72-73 23.345437053926176 26.399597454248795 26.85219676573941 23.40276872608561 74-75 23.24102923088222 26.693930914838816 26.759467004018177 23.305572850260788 76-77 23.30659840238828 26.358211463555513 27.119998200321426 23.215191933734776 78-79 23.31268555049984 26.537683085866504 27.107096092911053 23.042535270722603 80-81 23.476967764132965 26.455510268896887 26.861204478085966 23.206317488884185 82-83 23.496438164194792 26.590387062859218 26.76496382916289 23.148210943783106 84-85 23.357121848788147 26.681272160092263 26.72954174988677 23.232064241232816 86-87 23.62605915521661 26.503560402449207 26.447649975584675 23.422730466749506 88-89 23.27334067982264 26.744705747299076 26.261041651021465 23.72091192185682 90-91 23.312850352271102 26.584359798218266 26.496953688656923 23.60583616085371 92-93 23.470659070909175 26.21525503351264 26.511774085763246 23.802311809814935 94-95 23.251292444225662 26.01962245774641 26.672502391025954 24.056582707001976 96-97 23.40680476994219 25.77559860816035 26.59871997861558 24.218876643281874 98-99 23.213438570420035 26.09940842183407 26.16110696111702 24.526046046628878 100-101 23.622410107845113 25.63083882582844 26.465837027803392 24.280914038523058 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 617.0 1 422.5 2 340.0 3 464.5 4 418.5 5 405.5 6 732.0 7 1637.5 8 2514.5 9 2629.5 10 2114.0 11 1642.5 12 1514.5 13 1537.5 14 1680.5 15 1903.5 16 2102.0 17 2274.0 18 2427.5 19 2661.5 20 2998.5 21 3461.0 22 4071.0 23 4737.5 24 5672.0 25 6922.0 26 8346.5 27 10100.5 28 11798.0 29 13234.0 30 14923.0 31 17981.0 32 21961.5 33 22996.5 34 22650.0 35 23684.0 36 25766.0 37 28543.5 38 31121.0 39 33135.0 40 35227.5 41 38365.0 42 40685.5 43 43216.0 44 46305.0 45 48982.5 46 51280.0 47 53858.5 48 55442.0 49 55454.5 50 55300.5 51 54622.0 52 54248.0 53 55360.0 54 57179.5 55 55593.0 56 52202.5 57 49168.0 58 45740.0 59 41385.0 60 35233.5 61 29740.0 62 25233.5 63 20026.0 64 15331.0 65 11934.5 66 9211.0 67 7110.5 68 5528.0 69 4464.0 70 3572.5 71 2625.5 72 1970.5 73 1527.5 74 1207.5 75 976.5 76 744.0 77 515.0 78 348.5 79 245.0 80 160.0 81 101.0 82 54.0 83 33.0 84 19.0 85 10.0 86 6.5 87 3.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.939758406384624E-4 2 0.03579507748211735 3 8.60140494025001E-4 4 0.0 5 0.07549386951404047 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.9774094023942343E-4 26-27 0.008965310533875972 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01035476825499328 36-37 2.3157628685288486E-4 38-39 0.004565361083671159 40-41 0.005359336924309621 42-43 2.646586135461541E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.3082326693269265E-5 68-69 8.270581673317317E-4 70-71 1.3232930677307706E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0013894577211173093 82-83 7.608935139451932E-4 84-85 0.005127760637456736 86-87 0.002315762868528849 88-89 9.92469800798078E-5 90-91 0.002977409402394234 92-93 7.278111872519239E-4 94-95 3.3082326693269265E-5 96-97 0.0 98-99 0.0 100-101 3.3082326693269265E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1511381.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.93450552748245 #Duplication Level Percentage of deduplicated Percentage of total 1 78.34836864872354 44.60725627899983 2 12.158146800257432 13.844361524063995 3 4.018638009001622 6.863975038093617 4 1.8036961530473503 4.107701943822928 5 1.0004174940670412 2.847913767287505 6 0.6046163492035548 2.065411972544163 7 0.40931844645779497 1.63130403466463 8 0.26625610835927377 1.212732789848563 9 0.19961300599384435 1.0228381013802528 >10 1.0745637893560491 10.892207838854977 >50 0.07073958718620273 2.808240771430688 >100 0.04027171586555768 4.388237112403285 >500 0.003608057976238056 1.4801058246249157 >1k 0.0015130565706804751 1.426629579976263 >5k 2.3277793395084232E-4 0.8010834220044146 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6572 0.4348341020563313 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5517 0.3650303927335331 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4257 0.2816629294664945 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1866 0.12346324321928091 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1862 0.12319858460573475 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1649 0.10910551343440206 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1629 0.10778222036667129 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1602 0.10599577472523473 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 6.616465338653853E-5 0.0 0.0 0.0 0.0 8 6.616465338653853E-5 0.0 0.0 0.0 0.0 9 6.616465338653853E-5 0.0 0.0 0.0 0.0 10-11 6.616465338653853E-5 0.0 0.0 0.0 0.0 12-13 6.616465338653853E-5 0.0 0.0 0.0 0.0 14-15 6.616465338653853E-5 0.0 0.0 0.0 0.0 16-17 9.92469800798078E-5 0.0 0.0 0.0 0.0 18-19 1.3232930677307706E-4 0.0 0.0 0.0 0.0 20-21 1.3232930677307706E-4 0.0 0.0 3.3082326693269265E-5 0.0 22-23 1.6541163346634635E-4 0.0 0.0 6.616465338653853E-5 3.3082326693269265E-5 24-25 3.308232669326927E-4 0.0 0.0 1.984939601596156E-4 6.616465338653853E-5 26-27 3.308232669326927E-4 0.0 0.0 4.962349003990391E-4 6.616465338653853E-5 28-29 4.3007024701250047E-4 0.0 0.0 0.0013894577211173093 6.616465338653853E-5 30-31 4.631525737057698E-4 0.0 0.0 0.005789407171322121 6.616465338653853E-5 32-33 4.631525737057698E-4 0.0 0.0 0.014291565131492323 6.616465338653853E-5 34-35 4.96234900399039E-4 0.0 0.0 0.027326001848640417 6.616465338653853E-5 36-37 5.293172270923082E-4 0.0 0.0 0.04899492583273179 6.616465338653853E-5 38-39 5.293172270923082E-4 0.0 0.0 0.0838636981674376 6.616465338653853E-5 40-41 5.293172270923082E-4 0.0 0.0 0.12170987990453763 6.616465338653853E-5 42-43 5.623995537855776E-4 0.0 0.0 0.1601846258488098 6.616465338653853E-5 44-45 6.616465338653854E-4 0.0 0.0 0.21093291499628486 6.616465338653853E-5 46-47 6.616465338653854E-4 0.0 0.0 0.2737562533868032 6.616465338653853E-5 48-49 6.616465338653854E-4 0.0 0.0 0.33952391885302247 9.92469800798078E-5 50-51 6.616465338653854E-4 0.0 0.0 0.41640724608818025 1.3232930677307706E-4 52-53 6.616465338653854E-4 0.0 0.0 0.49554017153848035 1.3232930677307706E-4 54-55 6.616465338653854E-4 0.0 0.0 0.5816534679210603 1.3232930677307706E-4 56-57 7.939758406384624E-4 0.0 0.0 0.6879139012598412 1.3232930677307706E-4 58-59 7.939758406384624E-4 0.0 0.0 0.7994675068695452 1.3232930677307706E-4 60-61 7.939758406384624E-4 0.0 0.0 0.9190932001924068 1.3232930677307706E-4 62-63 7.939758406384624E-4 0.0 0.0 1.0374617651009244 1.3232930677307706E-4 64-65 7.939758406384624E-4 0.0 0.0 1.1697910718740014 1.3232930677307706E-4 66-67 7.939758406384624E-4 0.0 0.0 1.3237893026311698 1.3232930677307706E-4 68-69 7.939758406384624E-4 0.0 0.0 1.4977361763843797 1.3232930677307706E-4 70-71 7.939758406384624E-4 0.0 0.0 1.6881911311575308 1.3232930677307706E-4 72-73 8.601404940250009E-4 0.0 0.0 1.881954318600009 1.6541163346634635E-4 74-75 8.601404940250009E-4 0.0 0.0 2.078562586138108 1.984939601596156E-4 76-77 8.601404940250009E-4 0.0 0.0 2.2902563946483383 1.984939601596156E-4 78-79 8.601404940250009E-4 0.0 0.0 2.5345693772781317 3.969879203192312E-4 80-81 0.0010255521274913472 0.0 0.0 2.7969122279557572 3.969879203192312E-4 82-83 0.0010586344541846165 0.0 0.0 3.070833892976027 3.969879203192312E-4 84-85 0.0010917167808778857 0.0 0.0 3.347402144131758 3.969879203192312E-4 86-87 0.0011247991075711552 0.0 0.0 3.633332693741684 3.969879203192312E-4 88-89 0.0011247991075711552 0.0 0.0 3.939641956594664 3.969879203192312E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2890 0.0 58.268383 1 GTATCAA 5155 0.0 42.817062 1 ATCAACG 7025 0.0 31.401806 3 TATCAAC 7045 0.0 31.12773 2 TCAACGC 7125 0.0 30.893328 4 CAACGCA 7265 0.0 30.36344 5 GTGGTAT 1235 0.0 29.658646 1 TGGTATC 1180 0.0 29.428516 2 AACGCAG 7640 0.0 29.094051 6 AGGTAAG 845 0.0 27.584614 2 GTAAGGT 905 0.0 26.26576 4 TAAGGTG 950 0.0 26.022457 5 ACGCAGA 8780 0.0 25.208273 7 CGCAGAG 8915 0.0 24.77327 8 AAGGTAA 905 0.0 23.653284 1 GGTAAGG 1045 0.0 22.747658 3 GCAGAGT 9645 0.0 22.455065 9 AGAGTAC 8545 0.0 21.705046 10-11 GTACATG 5425 0.0 21.132206 1 GAGTACT 6220 0.0 21.036957 12-13 >>END_MODULE