FastQCFastQC Report
Thu 26 May 2016
SRR938305_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938305_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533047
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61420.40064003256260244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47390.3091229427408292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35200.2296080942071574No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26390.1721408410831501No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21180.13815623395760207No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21060.13737347909098677No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA19880.12967638956926955No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA17340.11310807822591219No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG15530.10130152565446461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23250.057.173421
GTATCAA44600.046.148951
ATCAACG58600.035.112983
TATCAAC59050.034.8453942
TCAACGC60050.034.423394
CAACGCA61800.033.3717275
AACGCAG64950.031.7480556
ACCGTCG1452.3981556E-829.4865768
ACGCAGA74200.027.5945457
CGCAGAG75700.027.0477568
GTACATG55200.026.1526971
TGGTATC10000.026.1360572
TACATGG54400.025.7691752
GTGGTAT11000.025.1216551
ACATGGG54500.024.6755773
GCAGAGT84800.023.9203599
TACCGTC1401.778201E-523.7530757
TAAGGTG8050.023.0221635
TACCGGG1452.3273738E-522.940742
GAGTACT50150.022.82761412-13
Produced by FastQC (version 0.11.5)