FastQCFastQC Report
Thu 26 May 2016
SRR938305_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938305_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533047
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64960.4237313011277541No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56080.36580744099822116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42590.27781274807621686No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA18730.1221749887642062No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC17850.11643478640902724No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16210.10573713656528469No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG16190.10560667742084881No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA15860.10345410153765672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28700.059.802581
GTATCAA52450.041.8784981
TGGTATC11200.032.2619172
GTGGTAT12200.031.9557231
TATCAAC72400.030.207442
ATCAACG72600.030.0489233
TCAACGC73650.029.6850594
CAACGCA74700.029.2678015
TAAGGTG8250.028.2289685
AACGCAG78300.027.9627786
GTAAGGT8300.026.3410194
AGGTAAG8100.024.6523842
ACGCAGA90150.024.181777
CGCAGAG91700.023.7730278
ATACCGT2002.0578227E-823.7471316
CGTACAC1652.4181863E-623.0440563
GGTAAGG10300.022.1491413
GAGTACT62550.021.2604212-13
GCAGAGT99600.021.172149
AGAGTAC90300.020.74915310-11
Produced by FastQC (version 0.11.5)