##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938305_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1533047 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.14240202681327 31.0 31.0 33.0 30.0 34.0 2 31.41528407152553 31.0 31.0 34.0 30.0 34.0 3 31.444740441747708 31.0 31.0 34.0 28.0 34.0 4 35.231966143242836 37.0 35.0 37.0 33.0 37.0 5 35.082070543173174 35.0 35.0 37.0 32.0 37.0 6 35.17167379734607 37.0 35.0 37.0 32.0 37.0 7 35.08942582973646 37.0 35.0 37.0 32.0 37.0 8 35.063542083184664 37.0 35.0 37.0 32.0 37.0 9 36.50185219370313 38.0 35.0 39.0 32.0 39.0 10-11 36.381638332027656 38.0 35.0 39.0 32.0 39.0 12-13 36.239257830973216 38.0 35.0 39.0 32.0 39.0 14-15 37.009939029918854 38.5 36.0 40.0 31.0 41.0 16-17 36.838331766736445 38.0 36.0 40.0 31.0 41.0 18-19 36.81611000836895 38.0 36.0 40.0 31.0 41.0 20-21 36.77448310456235 38.5 35.5 40.0 30.5 41.0 22-23 36.778754010803326 38.5 35.5 40.0 30.5 41.0 24-25 36.62251026876541 38.0 35.0 40.0 30.0 41.0 26-27 36.306678138374096 38.0 35.0 40.0 30.0 41.0 28-29 36.09722891731303 38.0 34.5 40.0 29.5 41.0 30-31 35.69995603526832 38.0 34.0 40.0 28.5 41.0 32-33 35.35150814032447 38.0 34.0 40.0 27.0 41.0 34-35 35.05767109553719 38.0 33.0 40.0 27.0 41.0 36-37 34.97128855149255 38.0 33.0 40.0 26.0 41.0 38-39 34.69623762350405 37.5 33.0 40.0 25.5 41.0 40-41 34.37437697604835 37.0 33.0 40.0 24.5 41.0 42-43 33.988667340270716 37.0 33.0 40.0 24.0 41.0 44-45 33.5112110065771 37.0 32.0 39.0 23.0 40.0 46-47 32.92184486189921 36.0 31.0 39.0 21.0 40.0 48-49 32.26286734848964 36.0 30.5 39.0 17.5 40.0 50-51 31.635197420561795 35.0 30.0 39.0 14.0 40.0 52-53 30.80473625400917 35.0 28.5 38.0 8.5 40.0 54-55 30.1754186270871 34.0 27.5 38.0 7.5 40.0 56-57 29.52884353839119 34.0 26.5 38.0 4.0 40.0 58-59 28.765076347952803 33.5 26.0 37.5 2.0 39.5 60-61 28.058938506125386 33.0 24.5 37.0 2.0 39.0 62-63 27.26758899107464 32.0 22.5 36.0 2.0 39.0 64-65 26.477318699296237 31.5 21.5 36.0 2.0 39.0 66-67 25.69689611603558 31.0 19.5 35.0 2.0 38.0 68-69 24.86280524993689 30.5 16.5 35.0 2.0 38.0 70-71 24.04542359105755 29.5 10.5 34.0 2.0 37.0 72-73 23.218255213310485 29.0 7.5 34.0 2.0 36.5 74-75 22.416649652619913 28.5 4.5 34.0 2.0 36.0 76-77 21.585798087077563 27.0 2.0 32.5 2.0 35.0 78-79 21.988116476533335 29.0 2.0 34.0 2.0 35.0 80-81 21.485314540258713 28.5 2.0 34.0 2.0 35.0 82-83 20.767618018234273 27.5 2.0 33.5 2.0 35.0 84-85 19.944185011940274 26.0 2.0 33.0 2.0 35.0 86-87 19.06140451010308 25.5 2.0 32.5 2.0 35.0 88-89 18.079871654293704 23.5 2.0 32.0 2.0 34.0 90-91 17.296164762071875 20.5 2.0 32.0 2.0 34.0 92-93 16.310149982355398 15.5 2.0 31.0 2.0 34.0 94-95 15.309856123132558 4.5 2.0 31.0 2.0 34.0 96-97 14.192961794387257 2.0 2.0 30.5 2.0 34.0 98-99 12.989139602373573 2.0 2.0 30.0 2.0 34.0 100-101 10.946540777940925 2.0 2.0 26.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33152387203741895 1101 2 0.2825487301808671 1101 3 0.35469749173676135 1101 4 0.3468439561413845 1101 5 0.40586564666685376 1101 6 0.43430537815561365 1101 7 0.3842749268784118 1101 8 0.3225422676027918 1101 9 0.4337236688884616 1101 10-11 0.46766276703619525 1101 12-13 0.3765025193318152 1101 14-15 0.5989996518642187 1101 16-17 0.7806550769295697 1101 18-19 0.7974715294233974 1101 20-21 0.7415417981052101 1101 22-23 0.77732221742815 1101 24-25 0.7061592588345889 1101 26-27 0.662474592851126 1101 28-29 0.7461471977753646 1101 30-31 0.9869850875322896 1101 32-33 0.9071225966173699 1101 34-35 0.8768888924872442 1101 36-37 0.9938105125035861 1101 38-39 1.2201348788102209 1101 40-41 1.4016237484404925 1101 42-43 1.4395069280984885 1101 44-45 1.3616292988512981 1101 46-47 1.5727363654446762 1101 48-49 1.5328175652840486 1101 50-51 1.7326528501715224 1101 52-53 1.8749311121897563 1101 54-55 1.7676251257880828 1101 56-57 1.9273428043975471 1101 58-59 2.3935345816955085 1101 60-61 2.2885122955478003 1101 62-63 2.7027379422711846 1101 64-65 2.9813354531427905 1101 66-67 3.1157088078519415 1101 68-69 2.9461932016144026 1101 70-71 3.187610402908497 1101 72-73 3.3074747255895964 1101 74-75 3.538934028061437 1101 76-77 3.3434984018608667 1101 78-79 3.7506845995556652 1101 80-81 4.176866558740372 1101 82-83 4.432178212810651 1101 84-85 4.795881856509666 1101 86-87 4.758841850355612 1101 88-89 4.651388973625547 1101 90-91 4.834132875931324 1101 92-93 4.870447924668188 1101 94-95 5.069512843927511 1101 96-97 5.248985171724584 1101 98-99 5.1887652740807955 1101 100-101 4.697786078346994 1102 1 0.4015066007876875 1102 2 0.3692178443895173 1102 3 0.43958052558117444 1102 4 0.42664744710445746 1102 5 0.25859106059399295 1102 6 0.24175372184410548 1102 7 0.5114919510514611 1102 8 0.380000995479449 1102 9 0.42460769157556655 1102 10-11 0.49946015611631367 1102 12-13 0.5385735686171884 1102 14-15 0.7404463635603022 1102 16-17 0.6709766115919891 1102 18-19 0.7620289553238351 1102 20-21 0.9129150701428017 1102 22-23 0.8021116193927043 1102 24-25 0.8452279914410781 1102 26-27 0.8230074423929494 1102 28-29 0.8009435537962517 1102 30-31 0.9463189318123426 1102 32-33 0.8925763588422484 1102 34-35 1.0101980853745474 1102 36-37 1.2234203216087067 1102 38-39 1.3136160204545675 1102 40-41 1.3299430374384826 1102 42-43 1.310824393499054 1102 44-45 1.2880516311533654 1102 46-47 1.581659397842003 1102 48-49 1.942076509419799 1102 50-51 1.7248248471647933 1102 52-53 1.9862713826074128 1102 54-55 2.0892656399326874 1102 56-57 2.532603300131992 1102 58-59 2.3803228795863802 1102 60-61 2.6278524909313283 1102 62-63 2.8059437086443673 1102 64-65 2.812517696315016 1102 66-67 2.9884763748835503 1102 68-69 3.3478020138374625 1102 70-71 3.512606014751565 1102 72-73 3.6301345650433454 1102 74-75 3.9286308365649525 1102 76-77 3.993420955915589 1102 78-79 4.463724929062707 1102 80-81 4.8176654310845315 1102 82-83 4.867218081393727 1102 84-85 4.998057310432678 1102 86-87 5.090005334994384 1102 88-89 4.953208082407624 1102 90-91 5.133514380285316 1102 92-93 5.288416522883178 1102 94-95 5.346606530743072 1102 96-97 5.404508487911727 1102 98-99 5.345194359454899 1102 100-101 4.588780575397216 1103 1 0.3067875398721256 1103 2 0.32409648911993116 1103 3 0.2829146755171088 1103 4 0.3072372736042013 1103 5 0.3442548110060386 1103 6 0.2858682189344748 1103 7 0.37478433881883433 1103 8 0.28649786011025213 1103 9 0.37710001829014317 1103 10-11 0.46825460698099164 1103 12-13 0.5117962715375839 1103 14-15 0.6222043379412057 1103 16-17 0.6788923125512625 1103 18-19 0.6625680413018316 1103 20-21 0.653421423610304 1103 22-23 0.6680651269146409 1103 24-25 0.6699952276346153 1103 26-27 0.6637256849957538 1103 28-29 0.752210335300326 1103 30-31 0.7797409798260162 1103 32-33 1.0504873141005504 1103 34-35 1.1683642823878344 1103 36-37 1.3554085266178575 1103 38-39 1.320911573732758 1103 40-41 1.2759712705113913 1103 42-43 1.5131214410317355 1103 44-45 1.547813025004217 1103 46-47 1.730968128777434 1103 48-49 1.7157354320430684 1103 50-51 1.8171524595769846 1103 52-53 2.2567570986888086 1103 54-55 2.2971090651487778 1103 56-57 2.6412785697711776 1103 58-59 2.892743148603497 1103 60-61 3.0222170351580537 1103 62-63 3.198257340478019 1103 64-65 3.3538360776975047 1103 66-67 3.294057511620025 1103 68-69 3.338488575679719 1103 70-71 3.450992065982046 1103 72-73 3.421927516523045 1103 74-75 3.7572664603616914 1103 76-77 3.6001315694834233 1103 78-79 4.028212592164113 1103 80-81 4.261220769164421 1103 82-83 4.705330356982994 1103 84-85 4.926698641896806 1103 86-87 4.971671922566035 1103 88-89 5.154760247280972 1103 90-91 5.268401631408569 1103 92-93 5.241942322181071 1103 94-95 4.933444842145709 1103 96-97 5.206844911936258 1103 98-99 4.9769523338358095 1103 100-101 4.313658822514844 1104 1 0.337648162492318 1104 2 0.2434741411708501 1104 3 0.358829691090655 1104 4 0.2452227481921554 1104 5 0.2247592281519175 1104 6 0.22112383673472635 1104 7 0.2537858497685832 1104 8 0.35671742424889175 1104 9 0.3788157691678222 1104 10-11 0.39543233600249295 1104 12-13 0.4516868297722638 1104 14-15 0.8036340107172606 1104 16-17 0.7575356380761633 1104 18-19 0.846328177720423 1104 20-21 0.7819950796260784 1104 22-23 0.6479720321158453 1104 24-25 0.7463077801497064 1104 26-27 0.7226913959650503 1104 28-29 0.7582810492333607 1104 30-31 0.8168901109371021 1104 32-33 0.9120346330619071 1104 34-35 1.1478504481022682 1104 36-37 1.1602373784569266 1104 38-39 1.2523347497961481 1104 40-41 1.3740951436411137 1104 42-43 1.652862018014062 1104 44-45 1.398309435725153 1104 46-47 1.6682504115613952 1104 48-49 2.088303959986966 1104 50-51 1.9994431014645748 1104 52-53 2.3968670601911413 1104 54-55 2.485555496968562 1104 56-57 2.759372100159087 1104 58-59 2.9642366871586994 1104 60-61 3.121607767257135 1104 62-63 3.0275735398144867 1104 64-65 3.130396804636341 1104 66-67 3.4119428378354364 1104 68-69 3.78427536470733 1104 70-71 3.8455915653020547 1104 72-73 4.2502231132368244 1104 74-75 4.074764520628911 1104 76-77 4.033456116786297 1104 78-79 4.703507202615032 1104 80-81 4.9159659680937615 1104 82-83 5.2114258202976025 1104 84-85 5.183516477786128 1104 86-87 5.439258276252023 1104 88-89 5.192551315476653 1104 90-91 5.299747651274899 1104 92-93 5.416203645951246 1104 94-95 5.500106373315701 1104 96-97 5.577213529225432 1104 98-99 5.532969465357736 1104 100-101 4.689654830439595 1105 1 0.20042252084668988 1105 2 0.3337036124503996 1105 3 0.18248668773619414 1105 4 0.17796085524506822 1105 5 0.14102365393839733 1105 6 0.16034922455508394 1105 7 0.2354084789533175 1105 8 0.08802352369426814 1105 9 0.22937012476739937 1105 10-11 0.18507479122668968 1105 12-13 0.24987408425496227 1105 14-15 0.4824940645739346 1105 16-17 0.49101849975762946 1105 18-19 0.4951455744956377 1105 20-21 0.4650957297676328 1105 22-23 0.4237035691842621 1105 24-25 0.3687656929721541 1105 26-27 0.6049187247668186 1105 28-29 0.6713514568755485 1105 30-31 0.637790366626966 1105 32-33 0.8926885759849057 1105 34-35 1.0263946037753513 1105 36-37 1.1179580275037893 1105 38-39 1.1714814758410839 1105 40-41 1.2245203310477777 1105 42-43 1.2025072641133008 1105 44-45 1.344564340056479 1105 46-47 1.4447521459338404 1105 48-49 1.5499318340920425 1105 50-51 1.6469791918696721 1105 52-53 1.93604517155066 1105 54-55 2.0538050228506144 1105 56-57 2.116981084130199 1105 58-59 2.6816542736412394 1105 60-61 2.6373183186293865 1105 62-63 2.7986542700854358 1105 64-65 2.8999016528794996 1105 66-67 3.351692717689467 1105 68-69 3.3366290828566143 1105 70-71 3.2843569699999904 1105 72-73 3.481535377869516 1105 74-75 3.7133734197911075 1105 76-77 3.7831138737046857 1105 78-79 4.202685879367344 1105 80-81 4.413314336063415 1105 82-83 4.473874085353742 1105 84-85 4.609600994384316 1105 86-87 4.685828176544401 1105 88-89 4.814026407217646 1105 90-91 5.055414210276343 1105 92-93 4.999305108093086 1105 94-95 4.769000116161829 1105 96-97 4.9817265064936365 1105 98-99 4.942080770145951 1105 100-101 4.688140750655457 1106 1 0.13830682870596078 1106 2 0.106629745337397 1106 3 0.10320586381492802 1106 4 0.001516885413856528 1106 5 0.10243736612186893 1106 6 0.16943616362964775 1106 7 0.02777432438238492 1106 8 0.06460226709986472 1106 9 0.032683556177865114 1106 10-11 0.24575104340821952 1106 12-13 0.10181462450662337 1106 14-15 0.0793310280766022 1106 16-17 0.14083267223301732 1106 18-19 0.3698233843839489 1106 20-21 0.6280802086710793 1106 22-23 0.4035398676732598 1106 24-25 0.39062023048091987 1106 26-27 0.23410909354741705 1106 28-29 0.49036401750705494 1106 30-31 0.5514468951291107 1106 32-33 0.4685923131214196 1106 34-35 0.4331548171742199 1106 36-37 0.5811204442071087 1106 38-39 0.6357606285320756 1106 40-41 0.8621038490807749 1106 42-43 0.9782838169897943 1106 44-45 0.39642607792725926 1106 46-47 0.0790381068722752 1106 48-49 0.8926708049999874 1106 50-51 0.7283682640791547 1106 52-53 1.0329966072117784 1106 54-55 1.06244039772006 1106 56-57 1.3470766051382128 1106 58-59 1.7701175355095842 1106 60-61 1.9351952711781486 1106 62-63 2.1645001734842175 1106 64-65 1.8939086242971968 1106 66-67 1.9249863631249227 1106 68-69 2.0928252046537317 1106 70-71 2.416012126733559 1106 72-73 2.763696915793478 1106 74-75 3.1804538200388954 1106 76-77 3.3668837770769073 1106 78-79 3.6231504971931905 1106 80-81 3.6022341900576578 1106 82-83 4.050739609976166 1106 84-85 4.048469225229759 1106 86-87 4.292962732874663 1106 88-89 4.430374707416771 1106 90-91 4.302079101886704 1106 92-93 3.927858353932166 1106 94-95 4.029456373093007 1106 96-97 4.423260800233621 1106 98-99 4.312187757343095 1106 100-101 3.6689367811910607 1107 1 0.17392125155759786 1107 2 0.20697791364441542 1107 3 0.21441360642269913 1107 4 0.2577989066578539 1107 5 0.15687745413882226 1107 6 0.16674879583120372 1107 7 0.2166211417963666 1107 8 0.24929433066474616 1107 9 0.1873046917144663 1107 10-11 0.21028834001648988 1107 12-13 0.2662952311223208 1107 14-15 0.4669789996070861 1107 16-17 0.452275367006564 1107 18-19 0.38447332367977793 1107 20-21 0.2089212849944886 1107 22-23 0.2466540816239089 1107 24-25 0.43099988958728375 1107 26-27 0.6213022343860075 1107 28-29 0.36866960933583215 1107 30-31 0.4814480256520852 1107 32-33 0.6307237257672185 1107 34-35 0.6752810523524957 1107 36-37 0.916324601910155 1107 38-39 1.1285991751055278 1107 40-41 1.1202622107727649 1107 42-43 1.36100205298073 1107 44-45 1.5262459970866757 1107 46-47 1.575809071110065 1107 48-49 1.6958588797849004 1107 50-51 1.4893372041892938 1107 52-53 1.9413270869303574 1107 54-55 1.8392302695449665 1107 56-57 1.8122414239186568 1107 58-59 1.9761188102151976 1107 60-61 2.194518726908342 1107 62-63 2.407498713634986 1107 64-65 2.653549445679481 1107 66-67 2.951060318988649 1107 68-69 3.0532162672961682 1107 70-71 3.222897667017044 1107 72-73 3.2895629537364535 1107 74-75 3.471760098373654 1107 76-77 3.4557804030392845 1107 78-79 3.8332067448072102 1107 80-81 4.263122355120565 1107 82-83 4.267390411090961 1107 84-85 4.368509792981673 1107 86-87 4.3638399695488275 1107 88-89 4.742619254281866 1107 90-91 4.716768036032921 1107 92-93 4.585242716997161 1107 94-95 4.4939125669662285 1107 96-97 4.648561740533815 1107 98-99 4.5278176470597575 1107 100-101 4.11050316173165 1108 1 0.14490227042297477 1108 2 0.2125349519057238 1108 3 0.12411478940752474 1108 4 0.1377122809138669 1108 5 0.006284997073869647 1108 6 0.026550463392851498 1108 7 0.10587974247226839 1108 8 0.20326081865643886 1108 9 0.21396284800736964 1108 10-11 0.2018847746629291 1108 12-13 0.3334827562434626 1108 14-15 0.44329647299073116 1108 16-17 0.47651465856618813 1108 18-19 0.49597872733838244 1108 20-21 0.5274856899810985 1108 22-23 0.38339526229357546 1108 24-25 0.5621095930211197 1108 26-27 0.5847927309566643 1108 28-29 0.778876269935914 1108 30-31 0.756633801537717 1108 32-33 0.7807272628243211 1108 34-35 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60-61 1.0277894212791132 2311 62-63 1.1794402205064607 2311 64-65 1.6806260307462004 2311 66-67 1.7004017634092676 2311 68-69 2.3469914968783137 2311 70-71 2.775562550448381 2311 72-73 2.764144326075236 2311 74-75 3.0478786647126412 2311 76-77 2.825940743735046 2311 78-79 2.976577736760081 2311 80-81 3.385973725841648 2311 82-83 2.839092272201757 2311 84-85 2.7968946482725023 2311 86-87 3.089407952574234 2311 88-89 2.9896949740656176 2311 90-91 2.8994029355284248 2311 92-93 2.9082848261808216 2311 94-95 2.847524182825735 2311 96-97 2.4534949820719127 2311 98-99 2.135646764960205 2311 100-101 2.053623597834264 2312 1 -0.12759716204013571 2312 2 -0.2648558918324504 2312 3 -0.10941453333178686 2312 4 -0.18998957303997344 2312 5 -0.16581942971387065 2312 6 -0.13476395204415326 2312 7 -0.20126365518191136 2312 8 -0.173689616455178 2312 9 -0.194585770986194 2312 10-11 -0.09701760899201162 2312 12-13 -0.13984452336304543 2312 14-15 -0.15648370105391507 2312 16-17 -0.19793520900854844 2312 18-19 -0.10003375847319518 2312 20-21 -0.14523924380626596 2312 22-23 -0.1559993650238667 2312 24-25 -0.2110992605543629 2312 26-27 -0.05378275144968825 2312 28-29 -0.3990423831098724 2312 30-31 -0.20466434450844417 2312 32-33 -0.3273537057719693 2312 34-35 0.01717718077553343 2312 36-37 -0.04745349062998372 2312 38-39 0.008428259147081008 2312 40-41 0.1560346375905155 2312 42-43 0.2013306648787463 2312 44-45 0.4465415589374686 2312 46-47 0.7892812146416972 2312 48-49 0.6596416937603493 2312 50-51 0.8680954667010958 2312 52-53 1.2447548489700182 2312 54-55 0.9588327246913373 2312 56-57 1.2649628592349949 2312 58-59 1.3694578092709122 2312 60-61 1.9500258090613158 2312 62-63 2.4728452669872034 2312 64-65 2.4171877837342493 2312 66-67 2.2954455881104643 2312 68-69 2.5803314703178906 2312 70-71 2.7630393100765325 2312 72-73 2.868295720497546 2312 74-75 2.7718866328401326 2312 76-77 2.901065577732389 2312 78-79 3.055519303825154 2312 80-81 2.84955672716967 2312 82-83 3.0338691646320406 2312 84-85 3.2665692830666586 2312 86-87 3.2334716975941546 2312 88-89 3.137697630108118 2312 90-91 3.146911567666539 2312 92-93 3.4606194875354035 2312 94-95 3.3533781004884187 2312 96-97 3.0919319010626154 2312 98-99 2.8265976281740155 2312 100-101 2.7435107160281547 2313 1 -0.27267231437595285 2313 2 -0.3644613984900893 2313 3 -0.3537823961167099 2313 4 -0.3589193857233397 2313 5 -0.3042026389217227 2313 6 -0.2759244124368365 2313 7 -0.15448866082397927 2313 8 -0.23253412108167737 2313 9 -0.22008836633713003 2313 10-11 -0.3093324363447465 2313 12-13 -0.26051299549123286 2313 14-15 -0.49439320229517136 2313 16-17 -0.3716077434251588 2313 18-19 -0.3613885316620937 2313 20-21 -0.443545720899472 2313 22-23 -0.09624084550231515 2313 24-25 -0.1340180147859087 2313 26-27 -0.10290597419850656 2313 28-29 -0.10744612944252907 2313 30-31 -0.20492681347707276 2313 32-33 -0.05214810884125143 2313 34-35 -0.055314807488962 2313 36-37 -0.012953603471324016 2313 38-39 0.05416263062078741 2313 40-41 -0.11186132268933591 2313 42-43 0.1434945105117933 2313 44-45 0.47907665259579346 2313 46-47 0.44592689281818565 2313 48-49 0.6263277691383635 2313 50-51 0.5223619979581464 2313 52-53 0.7970974352936473 2313 54-55 0.8974174685414802 2313 56-57 0.7816660864974629 2313 58-59 1.3126728848746119 2313 60-61 1.4274489641053236 2313 62-63 1.6172781263868892 2313 64-65 1.9776172578935807 2313 66-67 1.9793914242648292 2313 68-69 2.155363065894509 2313 70-71 2.557467204457037 2313 72-73 2.764661777820951 2313 74-75 2.3923095622014507 2313 76-77 2.519681465793777 2313 78-79 2.9997619127170516 2313 80-81 3.1971744206925763 2313 82-83 3.11254011926982 2313 84-85 3.263363460453256 2313 86-87 3.366972036118188 2313 88-89 3.3289655154613094 2313 90-91 2.916174036635166 2313 92-93 2.9722159668854395 2313 94-95 2.635173180605774 2313 96-97 2.442832149313574 2313 98-99 2.3855748342223126 2313 100-101 2.048048066513374 2314 1 -0.2819084626797945 2314 2 -0.34470237370878465 2314 3 -0.123926260410677 2314 4 -0.34422980047423124 2314 5 -0.2601136727842359 2314 6 -0.18661469831280897 2314 7 -0.4363596040092048 2314 8 -0.24744938902092173 2314 9 -0.05414085272037994 2314 10-11 -0.344422726262799 2314 12-13 -0.17278979699488417 2314 14-15 -0.30365427674688306 2314 16-17 -0.27680585577471106 2314 18-19 -0.045599501188199554 2314 20-21 -0.1323124492078307 2314 22-23 -0.2731777587694282 2314 24-25 -0.2276173842217375 2314 26-27 -0.222585167938675 2314 28-29 -0.14608502703311643 2314 30-31 -0.12976100406778812 2314 32-33 -0.17355697513941948 2314 34-35 0.05430298034909242 2314 36-37 0.018781761679896647 2314 38-39 -0.02508062500358932 2314 40-41 0.14092518485419703 2314 42-43 0.14725532728102309 2314 44-45 0.2657391424484885 2314 46-47 0.1863025366033213 2314 48-49 0.5885983391050544 2314 50-51 0.7205012947039329 2314 52-53 0.5558493763332031 2314 54-55 0.8189556813366785 2314 56-57 1.3359394050772089 2314 58-59 1.521584319576526 2314 60-61 1.6572141530556301 2314 62-63 2.1359255797093084 2314 64-65 2.3889795392424205 2314 66-67 2.4035536193826736 2314 68-69 2.5068453295929416 2314 70-71 2.898199224788687 2314 72-73 2.8503184639232764 2314 74-75 3.012126860274389 2314 76-77 3.3464380013286927 2314 78-79 3.439657896575685 2314 80-81 3.341680394547069 2314 82-83 3.2630283686121366 2314 84-85 3.602827278802266 2314 86-87 3.829335237039782 2314 88-89 3.7923358674926213 2314 90-91 4.085500053807262 2314 92-93 4.425188073178617 2314 94-95 4.456361753651178 2314 96-97 4.134421595447833 2314 98-99 4.037767493134927 2314 100-101 3.2860018318774795 2315 1 -0.26876358324779304 2315 2 -0.410807781866815 2315 3 -0.36520147491254207 2315 4 -0.3379797546796368 2315 5 -0.4020256152808841 2315 6 -0.5124340551369357 2315 7 -0.4044938957317399 2315 8 -0.2797180897001468 2315 9 -0.282879339960175 2315 10-11 -0.5005566369743377 2315 12-13 -0.4514370614091945 2315 14-15 -0.32684648946824524 2315 16-17 -0.38588857179695424 2315 18-19 -0.283343036823716 2315 20-21 -0.17856864979546572 2315 22-23 -0.4060828210867058 2315 24-25 -0.3675455050315364 2315 26-27 -0.33800219180315594 2315 28-29 -0.34987645282023294 2315 30-31 -0.3103840303209182 2315 32-33 -0.35807437342047166 2315 34-35 -0.10200494980866637 2315 36-37 -0.22660763888722357 2315 38-39 -0.15290065592409974 2315 40-41 0.07819025860180773 2315 42-43 0.1673866295326505 2315 44-45 0.5097968363061511 2315 46-47 0.441180576448275 2315 48-49 0.46097797260178197 2315 50-51 0.9316619860923545 2315 52-53 1.0906605927108117 2315 54-55 0.990036946586276 2315 56-57 1.1189756231034274 2315 58-59 1.5063365525207892 2315 60-61 2.03729826856058 2315 62-63 1.8738339758728202 2315 64-65 2.1688083041073476 2315 66-67 2.3772217294948526 2315 68-69 2.4309966642305056 2315 70-71 2.9058817253931757 2315 72-73 3.1087208554999997 2315 74-75 3.2578404865465593 2315 76-77 3.0532005801869815 2315 78-79 3.313228189441258 2315 80-81 3.542751621622294 2315 82-83 3.4508338185348357 2315 84-85 3.7355015363803545 2315 86-87 3.84740395090785 2315 88-89 3.9630123085568165 2315 90-91 4.111837439046017 2315 92-93 4.494722580318911 2315 94-95 4.335321153998484 2315 96-97 4.346228906462716 2315 98-99 4.675545099946232 2315 100-101 4.201628045434145 2316 1 -0.5420677502754288 2316 2 -0.8697643885644766 2316 3 -0.6675648601291719 2316 4 -0.6157346710791884 2316 5 -0.6034860963805357 2316 6 -0.4492476121748723 2316 7 -0.4945316290381214 2316 8 -0.29375497593230193 2316 9 -0.3244942677182223 2316 10-11 -0.4643117266390675 2316 12-13 -0.36511249721926475 2316 14-15 -0.7276503677205781 2316 16-17 -0.46768357502161706 2316 18-19 -0.3004030395189474 2316 20-21 -0.3945725771396056 2316 22-23 -0.39361374410884054 2316 24-25 -0.4797365154563238 2316 26-27 -0.41788405863923117 2316 28-29 -0.37596068376347347 2316 30-31 -0.36859571705745964 2316 32-33 -0.3745399802817744 2316 34-35 -0.33196334210028766 2316 36-37 -0.25172146448618804 2316 38-39 -0.5989279500032509 2316 40-41 -0.10882483953366773 2316 42-43 0.1996247825258095 2316 44-45 -0.0018636698206933033 2316 46-47 -0.15239525594653713 2316 48-49 0.12372992905446978 2316 50-51 0.4409876235638386 2316 52-53 0.46960779014649034 2316 54-55 0.7602837050834914 2316 56-57 0.8009497873395688 2316 58-59 1.0390199007741785 2316 60-61 1.5299669855319031 2316 62-63 1.659060953261715 2316 64-65 1.7754819013813083 2316 66-67 2.163710293992814 2316 68-69 2.57694911737671 2316 70-71 2.680065453867382 2316 72-73 2.8798904917393777 2316 74-75 3.0932722537551633 2316 76-77 3.2563335515886003 2316 78-79 3.375581395328421 2316 80-81 3.6323802565814347 2316 82-83 3.955326680971904 2316 84-85 4.0816673222823425 2316 86-87 4.274452089751019 2316 88-89 4.212478675859749 2316 90-91 4.378862548058695 2316 92-93 4.212691382960241 2316 94-95 4.437149342318483 2316 96-97 4.227026672304447 2316 98-99 3.9016074320955862 2316 100-101 4.021795029753644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 26.0 4 61.0 5 142.0 6 315.0 7 791.0 8 1823.0 9 2995.0 10 4560.0 11 6973.0 12 9958.0 13 13915.0 14 18365.0 15 23715.0 16 29608.0 17 35084.0 18 35898.0 19 35570.0 20 37175.0 21 38917.0 22 41953.0 23 44920.0 24 48415.0 25 52750.0 26 57739.0 27 64783.0 28 71415.0 29 77026.0 30 83015.0 31 89236.0 32 96425.0 33 102698.0 34 109868.0 35 113362.0 36 102886.0 37 65301.0 38 15073.0 39 286.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.753867790007604 20.821097344763302 15.729991141818314 21.695043723410777 2 18.614174584718256 21.897082946728837 36.138679111090234 23.350063357462673 3 21.725324436008076 24.029953458639504 29.83193578792534 24.41278631742708 4 16.037146936786673 17.81289158127572 33.636411669048634 32.51354981288897 5 14.63142989389406 37.24009659407314 33.34630307010643 14.78217044192637 6 34.22902233264864 35.67529240786486 15.47552031999019 14.620164939496311 7 29.61096430833497 32.55347031108635 19.341416147058766 18.49414923351991 8 27.518138713294505 35.24803870983734 18.286393046005763 18.947429530862394 9 26.661609200500703 16.276409007682087 18.862565857406853 38.19941593441036 10-11 26.189412327215017 26.25747938582444 26.046396490127176 21.506711796833365 12-13 26.912743053539778 23.240513826386273 26.857558835443403 22.989184284630543 14-15 23.196549094711383 24.728172065174782 25.04893848655651 27.026340353557327 16-17 22.987260012250115 27.94725145413024 26.033252731325263 23.032235802294384 18-19 23.20705105583847 25.985928676681148 28.55147298158504 22.255547285895343 20-21 23.3249535076224 25.18145236251726 29.049468150682923 22.444125979177414 22-23 23.341326130249104 24.99264536573243 29.13697362181329 22.529054882205177 24-25 23.408520959941136 25.38421421869022 28.85870251198361 22.34856230938503 26-27 22.75075943722872 25.666613498721524 28.877990003773945 22.704637060275815 28-29 22.95506921836056 25.922884295132505 28.780265706139474 22.341780780367465 30-31 22.977051584198005 25.652964325294658 28.61836590789454 22.751618182612795 32-33 22.673584079209963 25.58502243419038 28.679822013718496 23.061571472881162 34-35 23.519708461106127 25.608033237834686 28.45214885002037 22.420109451038815 36-37 23.513479839764432 25.316782268412187 28.50081507221494 22.66892281960844 38-39 23.088464965900744 25.60598429881569 28.398019933745545 22.907530801538023 40-41 23.389949698261326 25.508741647634725 28.49250040281836 22.608808251285595 42-43 23.23526860004162 25.67041560581539 28.19085472811874 22.903461066024253 44-45 23.189700496932172 25.722078061012155 28.35497708423842 22.733244357817256 46-47 23.652217985560124 26.083096631445947 27.635887104533357 22.628798278460568 48-49 23.496931274775008 26.27453691895943 27.34195363873384 22.886578167531717 50-51 23.506128644457736 26.377045191699928 27.357282588205056 22.759543575637274 52-53 23.991567120903664 26.082011836558173 27.241076105298795 22.685344937239368 54-55 23.570550534507596 26.432596793378416 27.318284213818693 22.678568458295295 56-57 23.51558693242934 26.50916769022737 27.159212992165276 22.816032385178016 58-59 23.53241616206157 26.19348917547864 27.080937505503744 23.193157156956048 60-61 23.165467203549532 26.362205464020345 27.45140886091555 23.020918471514573 62-63 23.3754738113052 26.347920187704617 27.486110993335494 22.79049500765469 64-65 23.16298847980525 26.388395137265853 27.270625101513524 23.177991281415377 66-67 22.94612472856238 26.521338959623392 27.252402251472724 23.280134060341503 68-69 23.6135907472915 26.122130951317086 27.209021534294862 23.05525676709655 70-71 23.399576138239727 26.305618810121285 26.87722555146711 23.417579500171883 72-73 23.35366213614525 26.476887687359778 26.776193677099812 23.39325649939516 74-75 23.308580884995695 26.738743169648423 26.554176095057752 23.398499850298133 76-77 23.41161099431394 26.42671751094389 27.009608968283427 23.152062526458746 78-79 23.386399764651703 26.570711791614997 27.004325372933774 23.038563070799526 80-81 23.522719971455512 26.474458846785698 26.848094202031188 23.15472697972761 82-83 23.563941737395584 26.5442920188164 26.753388481397394 23.138377762390622 84-85 23.40342672777211 26.59603510421053 26.768477183469958 23.232060984547402 86-87 23.72275319890621 26.42215646982696 26.477961206086793 23.377129125180033 88-89 23.395409075980407 26.76406097271022 26.139422385426663 23.701107565882705 90-91 23.358180599563283 26.58267192639425 26.462680993390553 23.59646648065191 92-93 23.532249258500904 26.252753510501663 26.443420047578574 23.77157718341886 94-95 23.328834667169367 25.99245163390294 26.611806422112306 24.06690727681539 96-97 23.481471866159353 25.76333276148742 26.585453674936254 24.169741697416974 98-99 23.330139258613727 26.046722637988267 26.123008622697153 24.500129480700853 100-101 23.71329542811985 25.584160043364975 26.41685493705458 24.285689591460596 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 608.0 1 425.0 2 353.5 3 485.0 4 418.0 5 386.5 6 744.5 7 1644.5 8 2522.0 9 2697.0 10 2152.0 11 1627.5 12 1594.0 13 1658.5 14 1789.5 15 1999.0 16 2103.0 17 2294.0 18 2497.0 19 2733.0 20 3127.0 21 3574.0 22 4160.0 23 4829.5 24 5792.0 25 7042.5 26 8561.5 27 10420.0 28 11923.0 29 13350.5 30 15250.5 31 18247.0 32 22097.5 33 23240.5 34 22839.0 35 24003.5 36 26210.0 37 28716.0 38 31391.0 39 33575.5 40 35499.5 41 38395.0 42 40881.5 43 43554.0 44 46721.5 45 49515.5 46 51751.0 47 54144.0 48 55607.0 49 55738.5 50 55577.0 51 55042.0 52 55116.0 53 56341.5 54 57537.5 55 55773.0 56 52772.0 57 49818.0 58 46609.0 59 42318.5 60 36174.0 61 30733.5 62 26198.5 63 20861.0 64 15868.0 65 12520.5 66 9769.0 67 7597.0 68 6018.5 69 4742.5 70 3711.5 71 2768.0 72 2142.5 73 1716.0 74 1303.0 75 1014.0 76 769.5 77 550.5 78 390.5 79 267.5 80 175.0 81 107.0 82 70.5 83 33.0 84 17.0 85 15.0 86 9.5 87 5.0 88 4.0 89 3.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.522957221794243E-5 2 0.030853587659086773 3 1.3045914443588486E-4 4 0.0 5 0.08323293415009456 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0010110583693781078 26-27 0.010991182918723301 28-29 0.0 30-31 0.0 32-33 4.892217916345683E-4 34-35 0.014056972812966596 36-37 0.003522396899768892 38-39 0.011023797704832273 40-41 0.006392498077358358 42-43 0.0052183657774353956 44-45 0.00267441246093564 46-47 3.2614786108971215E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 3.2614786108971215E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.913774333076546E-4 68-69 2.609182888717697E-4 70-71 0.0 72-73 3.2614786108971215E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.784435832691365E-5 82-83 0.0014676653749037048 84-85 0.004468225696929057 86-87 0.001500280161012676 88-89 3.2614786108971215E-5 90-91 9.458287971601653E-4 92-93 2.609182888717697E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.153696527242804E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1533047.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.44265781896315 #Duplication Level Percentage of deduplicated Percentage of total 1 77.7535284976931 43.886158032122914 2 12.548180141870361 14.165052759965945 3 4.106783504749884 6.953933282854797 4 1.8556633367900108 4.189542829825359 5 1.0155442471775964 2.86600082217308 6 0.6032318154353435 2.042880416647742 7 0.4019463885775104 1.5880845730433895 8 0.291792854353306 1.3175651385825762 9 0.20057434980496133 1.0188854453972205 >10 1.1052944715795852 11.058172245152862 >50 0.07044454678228358 2.767141843575521 >100 0.041211578654219726 4.314418104000227 >500 0.003946901241671077 1.5538458336964478 >1k 0.0016251946289233849 1.485244670387142 >5k 2.3217066127476926E-4 0.7930740025748176 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6496 0.4237313011277541 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5608 0.36580744099822116 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4259 0.27781274807621686 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1873 0.1221749887642062 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1785 0.11643478640902724 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1621 0.10573713656528469 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1619 0.10560667742084881 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1586 0.10345410153765672 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 6.522957221794243E-5 0.0 0.0 0.0 0.0 3 6.522957221794243E-5 0.0 0.0 0.0 0.0 4 6.522957221794243E-5 0.0 0.0 0.0 0.0 5 6.522957221794243E-5 0.0 0.0 0.0 0.0 6 6.522957221794243E-5 0.0 0.0 0.0 0.0 7 6.522957221794243E-5 0.0 0.0 0.0 0.0 8 6.522957221794243E-5 0.0 0.0 0.0 0.0 9 6.522957221794243E-5 0.0 0.0 0.0 0.0 10-11 6.522957221794243E-5 0.0 0.0 0.0 0.0 12-13 6.522957221794243E-5 0.0 0.0 0.0 0.0 14-15 6.522957221794243E-5 0.0 0.0 0.0 0.0 16-17 6.522957221794243E-5 0.0 0.0 0.0 0.0 18-19 6.522957221794243E-5 0.0 0.0 0.0 0.0 20-21 6.522957221794243E-5 0.0 0.0 3.2614786108971215E-5 0.0 22-23 6.522957221794243E-5 0.0 0.0 9.784435832691364E-5 6.522957221794243E-5 24-25 2.609182888717697E-4 0.0 0.0 1.3045914443588486E-4 6.522957221794243E-5 26-27 2.609182888717697E-4 0.0 0.0 4.892217916345683E-4 6.522957221794243E-5 28-29 3.587626471986834E-4 0.0 0.0 0.0013372062304678198 6.522957221794243E-5 30-31 3.913774333076546E-4 0.0 0.0 0.005414054494089222 6.522957221794243E-5 32-33 3.913774333076546E-4 0.0 0.0 0.012817610940825688 6.522957221794243E-5 34-35 4.892217916345682E-4 0.0 0.0 0.025341688806670634 6.522957221794243E-5 36-37 5.218365777435394E-4 0.0 0.0 0.0468022180663737 6.522957221794243E-5 38-39 5.218365777435394E-4 0.0 0.0 0.08029760340028713 6.522957221794243E-5 40-41 5.870661499614818E-4 0.0 0.0 0.11698923777287976 6.522957221794243E-5 42-43 6.522957221794243E-4 0.0 0.0 0.15922538578399748 6.522957221794243E-5 44-45 7.175252943973668E-4 0.0 0.0 0.20971307468068492 9.784435832691364E-5 46-47 7.50140080506338E-4 0.0 0.0 0.27494264689862735 1.6307393054485608E-4 48-49 7.827548666153092E-4 0.0 0.0 0.3462385693328385 1.956887166538273E-4 50-51 7.827548666153092E-4 0.0 0.0 0.4246445151388053 2.609182888717697E-4 52-53 7.827548666153092E-4 0.0 0.0 0.5040941341002592 2.609182888717697E-4 54-55 7.827548666153092E-4 0.0 0.0 0.5880772083308601 2.609182888717697E-4 56-57 7.827548666153092E-4 0.0 0.0 0.686736936310498 2.609182888717697E-4 58-59 7.827548666153092E-4 0.0 0.0 0.8003342363280448 2.609182888717697E-4 60-61 7.827548666153092E-4 0.0 0.0 0.9193129760535717 2.609182888717697E-4 62-63 7.827548666153092E-4 0.0 0.0 1.0377046496291373 2.609182888717697E-4 64-65 7.827548666153092E-4 0.0 0.0 1.1657176851068494 2.609182888717697E-4 66-67 8.479844388332517E-4 0.0 0.0 1.3209640669855522 2.609182888717697E-4 68-69 8.479844388332517E-4 0.0 0.0 1.489158518949517 2.609182888717697E-4 70-71 8.479844388332517E-4 0.0 0.0 1.6684746129766408 2.609182888717697E-4 72-73 8.479844388332517E-4 0.0 0.0 1.8585862012058338 3.2614786108971217E-4 74-75 8.479844388332517E-4 0.0 0.0 2.0559382719512187 3.913774333076546E-4 76-77 8.479844388332517E-4 0.0 0.0 2.272239533425916 4.5660700552559706E-4 78-79 8.80599224942223E-4 3.2614786108971215E-5 0.0 2.520503285287405 4.5660700552559706E-4 80-81 0.001043673155487079 6.522957221794243E-5 0.0 2.7833458465396035 4.5660700552559706E-4 82-83 0.001043673155487079 6.522957221794243E-5 0.0 3.0471342365889633 5.218365777435394E-4 84-85 0.0011089027277050214 6.522957221794243E-5 0.0 3.3133687355964954 5.218365777435394E-4 86-87 0.001174132299922964 6.522957221794243E-5 0.0 3.5930079116948144 5.544513638525107E-4 88-89 0.0012393618721409064 6.522957221794243E-5 0.0 3.89335747697233 5.87066149961482E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2870 0.0 59.80258 1 GTATCAA 5245 0.0 41.878498 1 TGGTATC 1120 0.0 32.261917 2 GTGGTAT 1220 0.0 31.955723 1 TATCAAC 7240 0.0 30.20744 2 ATCAACG 7260 0.0 30.048923 3 TCAACGC 7365 0.0 29.685059 4 CAACGCA 7470 0.0 29.267801 5 TAAGGTG 825 0.0 28.228968 5 AACGCAG 7830 0.0 27.962778 6 GTAAGGT 830 0.0 26.341019 4 AGGTAAG 810 0.0 24.652384 2 ACGCAGA 9015 0.0 24.18177 7 CGCAGAG 9170 0.0 23.773027 8 ATACCGT 200 2.0578227E-8 23.747131 6 CGTACAC 165 2.4181863E-6 23.044056 3 GGTAAGG 1030 0.0 22.149141 3 GAGTACT 6255 0.0 21.26042 12-13 GCAGAGT 9960 0.0 21.17214 9 AGAGTAC 9030 0.0 20.749153 10-11 >>END_MODULE