FastQCFastQC Report
Thu 26 May 2016
SRR938304_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938304_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497175
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57630.38492494197405114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43360.28961210279359456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32530.21727586955432732No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25470.17012039340758428No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21140.14119925860370364No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA19450.12991133301050312No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA19390.12951057825571494No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA17170.11468265232855211No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG15050.10052265099270294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22750.055.1180271
GTATCAA44000.044.535721
ATCAACG57800.033.9594083
TATCAAC58000.033.7603652
TCAACGC58900.033.163814
CAACGCA59750.032.6920245
TGGTATC10200.031.6844842
AACGCAG62000.031.649416
GTGGTAT11450.028.7318061
ACGCAGA71350.027.1653587
CGCAGAG72850.026.5416898
GTACATG52400.023.4751151
TACATGG51600.023.3028342
GCAGAGT81350.023.3004659
TAAGGTG6950.023.2504845
GAGTACT49000.022.39422812-13
AGAGTAC73250.021.79009210-11
GTGTAAG4600.021.766111
ACATGGG53450.021.6960183
AGGTAAG6600.020.8829562