##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938304_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1497175 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.98214704359878 33.0 31.0 34.0 31.0 34.0 2 32.205255898609046 33.0 31.0 34.0 31.0 34.0 3 32.28646951759146 34.0 31.0 34.0 31.0 34.0 4 35.922305675689216 37.0 35.0 37.0 35.0 37.0 5 35.9112882595555 37.0 35.0 37.0 35.0 37.0 6 35.92173727186201 37.0 36.0 37.0 35.0 37.0 7 35.87834421493813 37.0 35.0 37.0 35.0 37.0 8 35.846454823250454 37.0 35.0 37.0 35.0 37.0 9 37.48990665753836 39.0 37.0 39.0 35.0 39.0 10-11 37.36383522300332 39.0 37.0 39.0 35.0 39.0 12-13 37.23043031041796 39.0 37.0 39.0 34.0 39.0 14-15 38.58980279526442 40.0 38.0 41.0 35.0 41.0 16-17 38.53397498622405 40.0 38.0 41.0 35.0 41.0 18-19 38.51724781672149 40.0 38.0 41.0 34.0 41.0 20-21 38.454359042864056 40.0 38.0 41.0 34.0 41.0 22-23 38.326983151602185 40.0 38.0 41.0 34.0 41.0 24-25 38.2058743967806 40.0 38.0 41.0 34.0 41.0 26-27 38.018167214921434 40.0 38.0 41.0 33.5 41.0 28-29 37.81368911449897 40.0 37.5 41.0 33.0 41.0 30-31 37.56106767745922 40.0 37.0 41.0 33.0 41.0 32-33 37.29391353716166 39.0 36.5 41.0 32.0 41.0 34-35 37.01763387713527 39.0 36.0 40.5 31.0 41.0 36-37 36.852687227612 39.0 36.0 40.0 31.0 41.0 38-39 36.646970461034954 39.0 36.0 40.0 30.5 41.0 40-41 36.385552791089886 39.0 35.0 40.0 30.0 41.0 42-43 35.99345834655267 38.5 35.0 40.0 29.5 41.0 44-45 35.56019937549051 38.0 35.0 40.0 28.5 41.0 46-47 35.09553926561691 38.0 34.0 40.0 26.5 41.0 48-49 34.61282315026634 38.0 33.5 40.0 25.0 41.0 50-51 33.87146692938367 37.5 33.0 39.5 24.0 40.5 52-53 33.873262978609716 37.5 33.0 39.0 24.0 40.0 54-55 33.870767278374274 37.5 33.0 40.0 23.5 41.0 56-57 33.464789687244306 37.0 33.0 40.0 22.0 41.0 58-59 33.0159864411308 36.5 32.0 39.0 20.0 41.0 60-61 32.48553475712592 36.0 31.5 39.0 17.5 40.0 62-63 31.79692654499307 35.0 31.0 39.0 11.0 40.0 64-65 31.15505335047673 35.0 30.0 38.0 7.5 40.0 66-67 30.479376826356305 34.5 29.0 38.0 2.0 40.0 68-69 29.672948052164912 34.0 28.5 37.0 2.0 39.0 70-71 28.862745170070298 34.0 27.0 36.0 2.0 39.0 72-73 28.007010870472726 33.0 26.0 35.5 2.0 38.5 74-75 27.11642493362499 32.5 24.5 35.0 2.0 37.5 76-77 26.22394609848548 32.0 22.5 35.0 2.0 37.0 78-79 25.287978693205538 31.0 19.0 34.5 2.0 36.0 80-81 24.375641458079386 31.0 15.0 34.0 2.0 36.0 82-83 23.416636999682737 30.0 7.0 34.0 2.0 35.0 84-85 22.46896822348757 29.0 2.0 34.0 2.0 35.0 86-87 21.502788919131028 29.0 2.0 34.0 2.0 35.0 88-89 20.39107118406332 26.5 2.0 33.0 2.0 35.0 90-91 19.21611601850151 25.5 2.0 32.5 2.0 34.0 92-93 18.003682602234207 23.5 2.0 32.0 2.0 34.0 94-95 16.664614357039092 18.5 2.0 31.5 2.0 34.0 96-97 15.122243558702223 2.0 2.0 31.0 2.0 34.0 98-99 13.23013074623875 2.0 2.0 30.5 2.0 34.0 100-101 9.249467163157279 2.0 2.0 20.5 2.0 29.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32182101984409783 1101 2 0.22243973501340264 1101 3 0.30693450395841637 1101 4 0.23904666992835644 1101 5 0.15990485774067764 1101 6 0.21719785682562787 1101 7 0.15112634337081232 1101 8 0.19244948556052321 1101 9 0.3201912671412117 1101 10-11 0.355144288752804 1101 12-13 0.3399033825049287 1101 14-15 0.3452164337202035 1101 16-17 0.42872524660873523 1101 18-19 0.2788812135598633 1101 20-21 0.30223527214062074 1101 22-23 0.45425898228818085 1101 24-25 0.3893156947099996 1101 26-27 0.42821765135905565 1101 28-29 0.42439594744082143 1101 30-31 0.5197683769888926 1101 32-33 0.5988870316989008 1101 34-35 0.6093669679588132 1101 36-37 0.6122367807981348 1101 38-39 0.6442800053713356 1101 40-41 0.7027897310440139 1101 42-43 0.882034425840672 1101 44-45 0.9880962318374529 1101 46-47 1.1986732944356149 1101 48-49 1.057203773823531 1101 50-51 0.999579810124068 1101 52-53 0.9263597343568577 1101 54-55 1.0997812930574256 1101 56-57 1.3940137428537867 1101 58-59 1.4218708793017 1101 60-61 1.3900377319424422 1101 62-63 1.235394142960228 1101 64-65 1.243203303054468 1101 66-67 1.471669958424421 1101 68-69 1.6000349319937683 1101 70-71 2.046453840580565 1101 72-73 1.940411743778224 1101 74-75 1.9179039828055622 1101 76-77 2.306462929370671 1101 78-79 2.402722533335396 1101 80-81 2.9387941613785387 1101 82-83 2.9015721562654804 1101 84-85 3.0275836957247293 1101 86-87 3.14592745598242 1101 88-89 3.376223534931224 1101 90-91 3.405404040268781 1101 92-93 3.2879410288414554 1101 94-95 2.826543889106315 1101 96-97 2.709565109286091 1101 98-99 2.6398070710114236 1101 100-101 2.236859818481536 1102 1 0.49409834710098366 1102 2 0.3463791492617787 1102 3 0.3400589594238639 1102 4 0.21598353743053877 1102 5 0.22002649838137955 1102 6 0.2992349186847534 1102 7 0.37937847124428004 1102 8 0.4477457123549726 1102 9 0.35038863997144887 1102 10-11 0.4872634496669548 1102 12-13 0.5232154929532982 1102 14-15 0.5101232764251193 1102 16-17 0.6014398769954354 1102 18-19 0.39349987182673374 1102 20-21 0.47935057942564896 1102 22-23 0.36203409789705177 1102 24-25 0.37796256824393737 1102 26-27 0.48791455505183734 1102 28-29 0.5337359715678929 1102 30-31 0.3647092321594556 1102 32-33 0.332917090005985 1102 34-35 0.3637441879842882 1102 36-37 0.6319807657857339 1102 38-39 0.677919674294337 1102 40-41 0.6084830491862263 1102 42-43 0.9765438420996375 1102 44-45 1.0691615089636528 1102 46-47 0.9495613046225984 1102 48-49 1.1119110655600082 1102 50-51 1.063792161843125 1102 52-53 1.0294309092110936 1102 54-55 1.0682862525105534 1102 56-57 1.138772807259656 1102 58-59 1.2576618785644769 1102 60-61 1.2244171301061044 1102 62-63 1.140279874479564 1102 64-65 1.574748608328914 1102 66-67 1.677757854678024 1102 68-69 1.6807525447543057 1102 70-71 1.3240625799411987 1102 72-73 1.4694295710016014 1102 74-75 1.6636339941989 1102 76-77 1.5866175771824764 1102 78-79 2.092233122438003 1102 80-81 2.301415634470157 1102 82-83 2.552288428633286 1102 84-85 2.720292796991398 1102 86-87 2.7441757302267717 1102 88-89 3.0798794561161316 1102 90-91 2.9788868832417705 1102 92-93 3.031375617675984 1102 94-95 3.2348149500271646 1102 96-97 3.175229273886318 1102 98-99 2.813596528822565 1102 100-101 2.4197865660185656 1103 1 0.4204330874395801 1103 2 0.34925278793494385 1103 3 0.16429796213503067 1103 4 0.2505302213401279 1103 5 0.2552046935196657 1103 6 0.29863426091526435 1103 7 0.25536430491131057 1103 8 0.2248261873669506 1103 9 0.1972652195700917 1103 10-11 0.36502138786813276 1103 12-13 0.33529724805560335 1103 14-15 0.39525748897263213 1103 16-17 0.2984767961134267 1103 18-19 0.447316346261033 1103 20-21 0.3616362627641294 1103 22-23 0.5092564659985115 1103 24-25 0.5096605588303547 1103 26-27 0.4594123592045847 1103 28-29 0.49318402287654095 1103 30-31 0.5181930472225105 1103 32-33 0.6447874660899586 1103 34-35 0.6419562300237658 1103 36-37 0.7469258203701017 1103 38-39 0.8666465095828144 1103 40-41 1.0275089660919505 1103 42-43 0.9720897842132956 1103 44-45 0.9689366725854498 1103 46-47 1.0331252730339031 1103 48-49 1.053047260606391 1103 50-51 1.2094204892574112 1103 52-53 1.2761253221091522 1103 54-55 1.2004388392777017 1103 56-57 1.2126793147137178 1103 58-59 1.4381201509020585 1103 60-61 1.5671765781574152 1103 62-63 1.55962203934207 1103 64-65 1.8156717832155174 1103 66-67 1.8204455848481125 1103 68-69 1.9369416173780998 1103 70-71 2.052070463984709 1103 72-73 2.1366704183557523 1103 74-75 2.019566720528715 1103 76-77 2.106897982610061 1103 78-79 2.6641815840320113 1103 80-81 2.8719694540627607 1103 82-83 3.1347626791239875 1103 84-85 3.1082446852887493 1103 86-87 3.14416539103566 1103 88-89 3.2762937261692393 1103 90-91 3.504027364949607 1103 92-93 3.677099131294174 1103 94-95 3.5512823274174856 1103 96-97 3.4121992672561046 1103 98-99 3.0544763775749697 1103 100-101 2.506681559224239 1104 1 0.2747155987076688 1104 2 0.19906385737395738 1104 3 0.18558013337923995 1104 4 0.10867251494180152 1104 5 0.20656721777513098 1104 6 0.18864292768930824 1104 7 0.24488172666108454 1104 8 0.3073471704194475 1104 9 0.40101174582990495 1104 10-11 0.3635826778113511 1104 12-13 0.3270670084282514 1104 14-15 0.40451419166137725 1104 16-17 0.3340530726729938 1104 18-19 0.29148985814525474 1104 20-21 0.2737576666281356 1104 22-23 0.2934796776135258 1104 24-25 0.352624493290115 1104 26-27 0.39342887209352995 1104 28-29 0.31072989414298746 1104 30-31 0.32410013122374437 1104 32-33 0.46505288924072374 1104 34-35 0.31712445236358633 1104 36-37 0.3746894730957919 1104 38-39 0.4689384111343955 1104 40-41 0.5630564812332395 1104 42-43 0.48376947660673153 1104 44-45 0.4760944308821635 1104 46-47 0.7869083582520702 1104 48-49 0.9118376448333763 1104 50-51 0.7880318368512889 1104 52-53 0.9425350534391583 1104 54-55 1.156518236131582 1104 56-57 1.3067796447669764 1104 58-59 1.3518199174871697 1104 60-61 1.4012682542194739 1104 62-63 1.5580861796057484 1104 64-65 1.4833016378233488 1104 66-67 1.7786424612302518 1104 68-69 2.0637571020143888 1104 70-71 2.0815033015990174 1104 72-73 2.319871065679404 1104 74-75 2.0329311928909135 1104 76-77 2.072689471275506 1104 78-79 2.5023541857771257 1104 80-81 2.637566771197662 1104 82-83 2.764443440163898 1104 84-85 2.9536300115966263 1104 86-87 3.06699243392724 1104 88-89 3.5387662749933426 1104 90-91 3.4954919347171405 1104 92-93 3.467599043092495 1104 94-95 3.551131170185844 1104 96-97 3.3576382176305177 1104 98-99 3.3696810693931756 1104 100-101 2.250437455314989 1105 1 0.4042470240724292 1105 2 0.3338562254996589 1105 3 0.3042389437047106 1105 4 0.2967897988924193 1105 5 0.2230655342734451 1105 6 0.3533104361068169 1105 7 0.3085391340684893 1105 8 0.29349335448229397 1105 9 0.3889676942025204 1105 10-11 0.38531738600439525 1105 12-13 0.4457503698199474 1105 14-15 0.5061836641641833 1105 16-17 0.5043462825495411 1105 18-19 0.5162688985492991 1105 20-21 0.43345884507930776 1105 22-23 0.44496326347210413 1105 24-25 0.5507586122575603 1105 26-27 0.5490686027332643 1105 28-29 0.652831465411225 1105 30-31 0.7306166856569689 1105 32-33 0.7635342304303947 1105 34-35 0.7704002660560647 1105 36-37 0.7166963473943255 1105 38-39 0.9562911047370832 1105 40-41 0.8716388325239208 1105 42-43 0.754153876157794 1105 44-45 0.8840594982222356 1105 46-47 0.941537987881695 1105 48-49 0.869010063464124 1105 50-51 0.9127933407794586 1105 52-53 1.018808201587305 1105 54-55 1.182517815256162 1105 56-57 1.1757856773146784 1105 58-59 1.0608441879697708 1105 60-61 1.394523725599953 1105 62-63 1.4280672402118135 1105 64-65 1.5353148252532058 1105 66-67 1.6870108675153297 1105 68-69 1.7284175958415453 1105 70-71 1.832857117536168 1105 72-73 1.9880002744336132 1105 74-75 2.2697472703319903 1105 76-77 2.4021156777850443 1105 78-79 2.6923829455975543 1105 80-81 2.920268791399682 1105 82-83 3.309136902565694 1105 84-85 3.430860651327265 1105 86-87 3.3791066314581037 1105 88-89 3.458656145924884 1105 90-91 3.3513210592962643 1105 92-93 3.522197107065555 1105 94-95 3.3139117537997613 1105 96-97 3.2639057541063874 1105 98-99 3.200734660863434 1105 100-101 2.343493010870544 1106 1 0.04562402942001853 1106 2 0.07829472817345362 1106 3 -0.12284661779261086 1106 4 -0.10407918506480485 1106 5 -0.027201845405699032 1106 6 -0.15973100774345284 1106 7 0.013465604656722974 1106 8 -0.28632616423428203 1106 9 0.04929523430946858 1106 10-11 -9.159027656622243E-4 1106 12-13 0.1964355302477543 1106 14-15 0.15189222031391836 1106 16-17 -0.1480668190547405 1106 18-19 0.14526288250651476 1106 20-21 0.17945483438412424 1106 22-23 0.024365001440024514 1106 24-25 0.18045112562654708 1106 26-27 0.0011006882947626195 1106 28-29 0.1830587381384987 1106 30-31 0.04106454661953052 1106 32-33 0.15777554059082632 1106 34-35 0.05380267247317505 1106 36-37 0.13066359338363753 1106 38-39 0.26262131864083216 1106 40-41 0.434650196160284 1106 42-43 0.04823141626474836 1106 44-45 0.15856818806179973 1106 46-47 0.37148451194587295 1106 48-49 0.32785698859781576 1106 50-51 0.13671045307891916 1106 52-53 0.25512170426110714 1106 54-55 0.1581294462722056 1106 56-57 0.6859494473681451 1106 58-59 0.6084611665793958 1106 60-61 0.8713920411079741 1106 62-63 1.0005104220300005 1106 64-65 0.9991985150927754 1106 66-67 1.149464076071478 1106 68-69 1.255489788354918 1106 70-71 1.0552568501564892 1106 72-73 1.2429835632036692 1106 74-75 1.4725380457330601 1106 76-77 1.8791544478385198 1106 78-79 2.1195453787526333 1106 80-81 2.075148470544068 1106 82-83 2.3855974640630215 1106 84-85 2.591622683412826 1106 86-87 2.1753700004420615 1106 88-89 2.068405477475686 1106 90-91 2.2673705245369042 1106 92-93 2.4978555295254488 1106 94-95 2.297347582676764 1106 96-97 2.130406422555586 1106 98-99 1.9122386715586206 1106 100-101 1.4110732247742863 1107 1 0.05085878387226117 1107 2 -3.493624903327941E-4 1107 3 0.03649499041030424 1107 4 0.0347902159065967 1107 5 -1.3087940462241932E-4 1107 6 -0.026876177738053286 1107 7 0.0026331707657405445 1107 8 -0.06219367637675077 1107 9 0.018257727563650406 1107 10-11 -0.0066316340122867246 1107 12-13 0.04619605372319313 1107 14-15 0.07089383931013771 1107 16-17 0.10725495644445715 1107 18-19 0.1525517384158519 1107 20-21 0.3070357841952429 1107 22-23 0.35642854704175164 1107 24-25 0.4256595713901774 1107 26-27 0.3539737320076597 1107 28-29 0.24207249960638677 1107 30-31 0.29508707765029385 1107 32-33 0.4527101061601755 1107 34-35 0.42998116680669085 1107 36-37 0.4797879862600496 1107 38-39 0.576036726088212 1107 40-41 0.3626547833162519 1107 42-43 0.671746661812513 1107 44-45 0.4928194023089745 1107 46-47 0.5122427418433304 1107 48-49 0.18820574736737683 1107 50-51 0.4730049754750354 1107 52-53 0.55200513653309 1107 54-55 0.7598557009942724 1107 56-57 1.0806829875732191 1107 58-59 0.9183478368438287 1107 60-61 1.0468824620469945 1107 62-63 1.2159347339566047 1107 64-65 1.2842983531931544 1107 66-67 1.342254820809739 1107 68-69 1.577427187167654 1107 70-71 1.6756062835078112 1107 72-73 1.592219936652132 1107 74-75 1.896887074335325 1107 76-77 1.823498079786713 1107 78-79 2.172838012319822 1107 80-81 2.525627423593175 1107 82-83 2.905292553107813 1107 84-85 2.801528916548282 1107 86-87 2.7169923695732017 1107 88-89 2.8724097947989407 1107 90-91 2.894101501331292 1107 92-93 2.830701589967976 1107 94-95 2.707427183324363 1107 96-97 2.9293952244983803 1107 98-99 2.746997171288788 1107 100-101 1.8522294579097434 1108 1 0.22476393494895675 1108 2 0.1419230287137765 1108 3 2.8293003595081245E-5 1108 4 -0.05123485842599962 1108 5 0.011721592253593371 1108 6 0.14947890152354404 1108 7 0.12513719877097174 1108 8 0.13580894977665992 1108 9 0.18875729296308918 1108 10-11 0.1630393084349393 1108 12-13 0.262010512406988 1108 14-15 0.3040166298697642 1108 16-17 0.27294857497116 1108 18-19 0.28745032940901183 1108 20-21 0.1474979580460669 1108 22-23 0.0896869846527224 1108 24-25 0.2399733331230678 1108 26-27 0.2636621114775508 1108 28-29 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1756.0 9 2307.0 10 3011.0 11 4234.0 12 5619.0 13 7495.0 14 9108.0 15 11370.0 16 14363.0 17 18058.0 18 17662.0 19 16553.0 20 18323.0 21 20418.0 22 23652.0 23 27042.0 24 31245.0 25 35718.0 26 41157.0 27 47510.0 28 54676.0 29 63787.0 30 74089.0 31 87592.0 32 104063.0 33 124032.0 34 148499.0 35 168149.0 36 166287.0 37 113455.0 38 27158.0 39 382.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22601918505408 17.667594442459915 14.112334369234214 24.994052003251795 2 15.730415901119091 18.90796726240187 39.60541172540504 25.756205111073992 3 19.421349827377806 22.382353314566615 28.96124002001843 29.23505683803715 4 13.207026462832957 15.114526158793035 34.847585789374506 36.830861588999504 5 14.666844892980668 36.184464234991395 33.34498487861117 15.803705993416767 6 34.62049931838229 34.09879447221405 16.248362781000242 15.032343428403411 7 30.112736588475368 31.570053441633156 19.374696809939422 18.94251315995205 8 27.510694683329124 33.95429985740422 18.794577885760336 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22.337244207014184 25.40014578087304 28.198477117680977 24.0641328944318 34-35 23.240803190634676 25.53887391761714 28.025251953308338 23.195070938439848 36-37 23.229466488143437 25.18934686689062 27.916736819881287 23.664449825084652 38-39 22.66795329009183 25.45156240240517 27.981287177889048 23.89919712961395 40-41 23.07303830141994 25.319078296930865 28.14118271065247 23.466700690996735 42-43 22.900476263799174 25.384803714450143 27.916894488813863 23.79782553293682 44-45 22.643127135313005 25.748238392865197 28.032741493901636 23.575892977920166 46-47 23.14565959382785 26.004691175419364 27.3768366031935 23.472812627559286 48-49 22.993260039294725 26.206549165504818 27.075215128937067 23.724975666263386 50-51 23.05259740520002 26.311461502183707 27.158458884938753 23.477482207677518 52-53 23.396694259040554 25.80275505132823 27.277506426048344 23.523044263582875 54-55 22.875080032370317 26.229242099715407 27.40295000068468 23.492727867229597 56-57 22.84144230380642 26.31143349640005 27.21515288047611 23.63197131931741 58-59 22.893441363694052 25.87839236929818 27.21099318019384 24.01717308681393 60-61 22.405017293872934 26.185388447505847 27.53974117304427 23.86985308557695 62-63 22.787854618279127 26.146278182254207 27.6337561130717 23.432111086394965 64-65 22.55516300917196 26.261586853726264 27.39159941832775 23.791650718774026 66-67 22.399231861067044 26.27562828755114 27.408032061451117 23.9171077899307 68-69 23.029574542200944 25.81038507255617 27.5299975318645 23.630042853378388 70-71 22.856771632687618 26.008925323811027 27.05403191032057 24.08027113318078 72-73 22.773251124333875 26.285815440573547 26.994033056167428 23.946900378925147 74-75 22.659573331053206 26.569079861482226 27.001012697462645 23.770334110001922 76-77 22.86697148124522 26.21938634078893 26.9915201875645 23.922121990401347 78-79 22.956941643985594 26.44589123839825 27.005233374292303 23.59193374332385 80-81 23.08409385742361 26.358756280920296 26.73571169957929 23.821438162076802 82-83 22.88655606596495 26.454485848358644 26.761677855554623 23.897280230121783 84-85 22.832818707551723 26.53221267933275 26.716208791667572 23.918759821447956 86-87 23.200307253007825 26.512227500814056 26.32129647410475 23.966168772073374 88-89 22.984420749976454 26.593614785490544 26.175658532446803 24.2463059320862 90-91 22.92741311038854 26.533875641112147 26.292496123430247 24.246215125069064 92-93 23.152306750429272 26.139430738035223 26.353156979402588 24.355105532132917 94-95 22.95857849809686 25.875403133543884 26.63478898432894 24.53122938403032 96-97 23.27053352613185 25.603121652455624 26.472685708025157 24.65365911338737 98-99 22.992119514572252 25.847384316117218 26.084660608728587 25.075835560581943 100-101 23.982862005038424 24.806963522416304 26.177702218143338 25.032472254401934 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 502.0 1 372.0 2 346.0 3 445.0 4 365.0 5 343.5 6 636.5 7 1363.0 8 2199.0 9 2427.0 10 1968.0 11 1521.5 12 1423.5 13 1464.0 14 1564.0 15 1697.5 16 1769.5 17 1848.5 18 2027.0 19 2232.5 20 2449.5 21 2919.5 22 3526.0 23 4152.5 24 4952.0 25 6082.0 26 7698.5 27 9507.0 28 10858.0 29 12191.5 30 13837.0 31 16571.0 32 20451.5 33 21538.5 34 21255.5 35 22573.5 36 24684.0 37 27012.5 38 29602.5 39 32018.5 40 34019.0 41 37050.0 42 39809.0 43 42194.0 44 44852.0 45 48368.5 46 51186.5 47 53175.5 48 54854.0 49 55145.0 50 54916.5 51 54362.5 52 54638.0 53 56134.0 54 57748.0 55 56443.0 56 53174.0 57 50259.0 58 48108.0 59 44412.5 60 37244.0 61 31055.5 62 26348.5 63 20850.5 64 16014.5 65 12701.5 66 9839.0 67 7365.5 68 5655.5 69 4515.5 70 3567.0 71 2625.0 72 2033.0 73 1646.5 74 1295.0 75 1016.5 76 761.5 77 556.5 78 377.0 79 257.5 80 163.5 81 89.5 82 61.5 83 39.5 84 23.5 85 15.0 86 14.0 87 12.0 88 9.5 89 8.5 90 7.0 91 3.5 92 2.5 93 2.5 94 0.5 95 0.0 96 1.0 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.339038522550804 2 0.028386795130829732 3 0.03700302235877569 4 0.04414981548583165 5 0.06345283617479586 6 0.049092457461552594 7 0.03934075842837344 8 0.04254679646667891 9 0.03827207908227161 10-11 0.03743717334312956 12-13 0.028987927263012008 14-15 0.027885851687344497 16-17 0.02277622856379515 18-19 0.026516606275151535 20-21 0.031926795464792025 22-23 0.030824719889124514 24-25 0.038004909245746156 26-27 0.032895286122196804 28-29 0.029355285788234507 30-31 0.03152604071000384 32-33 0.027251323325596537 34-35 0.028019436605607226 36-37 0.030123399068245196 38-39 0.01559603920717351 40-41 0.013826039040192362 42-43 0.014059812647152137 44-45 0.011722076577554395 46-47 0.02150717184029923 48-49 0.018835473475044665 50-51 0.012089435102776897 52-53 0.009551321655785063 54-55 0.008315661161854827 56-57 0.009317548048825288 58-59 0.004341509843538665 60-61 0.00754754788184414 62-63 0.011889057725382804 64-65 0.007480755422712775 66-67 0.003339622956568203 68-69 0.00624509492878254 70-71 0.013258303137575768 72-73 0.011855661495817123 74-75 0.012223020021039624 76-77 0.0060113213218227655 78-79 0.009918680181007564 80-81 0.012089435102776897 82-83 0.015262076911516688 84-85 0.008850000834905739 86-87 0.0025047172174261527 88-89 0.01656452986457829 90-91 0.024178870205553794 92-93 0.011120944445372118 94-95 0.020538681182894453 96-97 0.018401322490690798 98-99 0.012423397398433718 100-101 0.11137642560154959 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1497175.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.12112309441781 #Duplication Level Percentage of deduplicated Percentage of total 1 76.96099597852628 42.421765328013414 2 12.84671267849562 14.1625046181995 3 4.217828700363286 6.974743649516792 4 1.9488979229433576 4.297017692360641 5 1.0765609254871307 2.9670623646208245 6 0.6675078935353155 2.2076270859633396 7 0.45530671959866287 1.7567912415699427 8 0.31523439576699 1.3900859146133344 9 0.22790257895122895 1.1306021497115368 >10 1.158362277434318 11.386408556969549 >50 0.07427706151059285 2.8674920776233703 >100 0.04361031759885819 4.278743022914113 >500 0.004858964134566607 1.9012023298866727 >1k 0.0018221115504624776 1.8720756477178797 >5k 1.2147410336416519E-4 0.38587832031897207 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5763 0.38492494197405114 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4336 0.28961210279359456 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3253 0.21727586955432732 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2547 0.17012039340758428 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2114 0.14119925860370364 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1945 0.12991133301050312 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1939 0.12951057825571494 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1717 0.11468265232855211 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1505 0.10052265099270294 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 6.679245913136407E-5 20-21 0.0 0.0 0.0 3.339622956568203E-5 6.679245913136407E-5 22-23 0.0 0.0 0.0 2.003773773940922E-4 6.679245913136407E-5 24-25 0.0 0.0 0.0 4.007547547881844E-4 6.679245913136407E-5 26-27 0.0 0.0 0.0 5.677359026165945E-4 6.679245913136407E-5 28-29 0.0 0.0 0.0 0.0015362265600213736 1.3358491826272813E-4 30-31 0.0 0.0 0.0 0.004942641975720941 1.3358491826272813E-4 32-33 0.0 0.0 0.0 0.012824152153221902 1.3358491826272813E-4 34-35 0.0 0.0 0.0 0.025347738240352663 1.3358491826272813E-4 36-37 0.0 0.0 0.0 0.04835774041110759 1.3358491826272813E-4 38-39 0.0 0.0 0.0 0.08175396997678962 2.003773773940922E-4 40-41 0.0 0.0 0.0 0.12036001135471805 2.003773773940922E-4 42-43 1.3358491826272813E-4 0.0 0.0 0.16043548683353648 2.003773773940922E-4 44-45 2.6716983652545626E-4 0.0 0.0 0.20936096314726066 2.003773773940922E-4 46-47 4.007547547881844E-4 0.0 0.0 0.27314776161771337 2.003773773940922E-4 48-49 4.007547547881844E-4 0.0 0.0 0.3448494664952327 2.003773773940922E-4 50-51 4.007547547881844E-4 0.0 0.0 0.42209494548065524 2.003773773940922E-4 52-53 4.007547547881844E-4 0.0 0.0 0.49990816036869434 2.003773773940922E-4 54-55 4.007547547881844E-4 0.0 0.0 0.5945530749578373 2.003773773940922E-4 56-57 4.675472139195485E-4 0.0 0.0 0.7018885567819393 2.003773773940922E-4 58-59 5.343396730509125E-4 0.0 0.0 0.8186751715731294 2.003773773940922E-4 60-61 5.343396730509125E-4 0.0 0.0 0.948753485731461 2.003773773940922E-4 62-63 5.343396730509125E-4 0.0 0.0 1.0808021774341676 2.003773773940922E-4 64-65 5.343396730509125E-4 0.0 0.0 1.2229365304657103 2.003773773940922E-4 66-67 5.343396730509125E-4 0.0 0.0 1.3960291883046403 2.003773773940922E-4 68-69 5.343396730509125E-4 0.0 0.0 1.5806769415732964 2.003773773940922E-4 70-71 5.343396730509125E-4 0.0 0.0 1.7810209227378229 3.005660660911383E-4 72-73 5.677359026165945E-4 0.0 0.0 1.987609998831132 3.6735852522250235E-4 74-75 6.011321321822766E-4 0.0 0.0 2.201846811494982 4.007547547881844E-4 76-77 6.011321321822766E-4 0.0 0.0 2.434217776812998 4.007547547881844E-4 78-79 6.011321321822766E-4 0.0 0.0 2.701587990715848 4.007547547881844E-4 80-81 7.347170504450048E-4 0.0 0.0 2.9800123566049392 4.3415098435386644E-4 82-83 7.347170504450048E-4 0.0 0.0 3.2794763471204105 5.009434434852305E-4 84-85 7.347170504450048E-4 0.0 0.0 3.584484111743784 5.343396730509125E-4 86-87 7.347170504450048E-4 0.0 0.0 3.9034181040960476 5.343396730509125E-4 88-89 7.347170504450048E-4 0.0 0.0 4.238649456476364 5.343396730509125E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2275 0.0 55.118027 1 GTATCAA 4400 0.0 44.53572 1 ATCAACG 5780 0.0 33.959408 3 TATCAAC 5800 0.0 33.760365 2 TCAACGC 5890 0.0 33.16381 4 CAACGCA 5975 0.0 32.692024 5 TGGTATC 1020 0.0 31.684484 2 AACGCAG 6200 0.0 31.64941 6 GTGGTAT 1145 0.0 28.731806 1 ACGCAGA 7135 0.0 27.165358 7 CGCAGAG 7285 0.0 26.541689 8 GTACATG 5240 0.0 23.475115 1 TACATGG 5160 0.0 23.302834 2 GCAGAGT 8135 0.0 23.300465 9 TAAGGTG 695 0.0 23.250484 5 GAGTACT 4900 0.0 22.394228 12-13 AGAGTAC 7325 0.0 21.790092 10-11 GTGTAAG 460 0.0 21.76611 1 ACATGGG 5345 0.0 21.696018 3 AGGTAAG 660 0.0 20.882956 2 >>END_MODULE