FastQCFastQC Report
Thu 26 May 2016
SRR938304_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938304_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497175
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62740.4190558885901782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56050.3743717334312956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41660.2782573847412627No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA17570.11735435069380666No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC17470.11668642610249302No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG16640.11114265199458982No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA15950.1065339723145257No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA15560.10392906640840249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28900.063.2910081
GTATCAA50650.040.6151051
TGGTATC10550.035.118362
GTGGTAT11600.033.1746371
GTAAGGT9100.031.8384234
AGGTAAG8400.029.404662
TATCAAC70900.029.0760632
ATCAACG71550.028.6108473
GGTAAGG9200.028.3947433
TCAACGC72650.028.1776474
TAAGGTG10650.027.6506395
CAACGCA74600.027.44115
AACGCAG77500.026.4142726
AAGGTAA9200.024.7874891
ACGCAGA87950.022.8977647
CGCAGAG88950.022.5869458
GAGTACA42950.020.5744821
AGAGTAC89700.020.5448210-11
AAGGTGT15150.020.3781056
AGGTGTG16200.020.2300597