##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938304_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1497175 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.13258904269708 31.0 31.0 33.0 30.0 34.0 2 31.42442600230434 31.0 31.0 34.0 30.0 34.0 3 31.447898208292283 31.0 31.0 34.0 30.0 34.0 4 35.222104964349526 37.0 35.0 37.0 33.0 37.0 5 35.07733364503147 35.0 35.0 37.0 32.0 37.0 6 35.16751615542605 37.0 35.0 37.0 32.0 37.0 7 35.07928064521516 37.0 35.0 37.0 32.0 37.0 8 35.03259805967906 37.0 35.0 37.0 32.0 37.0 9 36.437735067710854 38.0 35.0 39.0 32.0 39.0 10-11 36.310011187736905 38.0 35.0 39.0 32.0 39.0 12-13 36.15080034064154 38.0 35.0 39.0 32.0 39.0 14-15 36.84893282348423 38.0 36.0 40.0 31.0 41.0 16-17 36.92677075158215 38.0 36.0 40.0 31.0 41.0 18-19 37.06993671414497 38.5 36.0 40.0 31.0 41.0 20-21 36.9666204685491 39.0 36.0 40.0 31.0 41.0 22-23 36.754331323993526 38.5 35.5 40.0 30.5 41.0 24-25 36.518771018751984 38.0 35.0 40.0 30.0 41.0 26-27 36.12397481924291 38.0 34.5 40.0 29.5 41.0 28-29 35.880007347170505 38.0 34.0 40.0 28.5 41.0 30-31 35.447391587489776 38.0 34.0 40.0 27.0 41.0 32-33 35.18922203483227 38.0 34.0 40.0 27.0 41.0 34-35 34.90410339472673 38.0 33.0 40.0 26.0 41.0 36-37 34.62174929450465 37.0 33.0 40.0 25.0 41.0 38-39 34.2159243241438 37.0 33.0 40.0 24.5 40.5 40-41 33.78983318583332 37.0 32.0 39.0 24.0 40.0 42-43 33.2989239734834 36.5 31.0 39.0 23.0 40.0 44-45 32.76208158698883 36.0 31.0 39.0 20.5 40.0 46-47 32.0399926528295 35.5 30.0 39.0 16.5 40.0 48-49 31.297598477131935 35.0 29.5 38.0 11.5 40.0 50-51 30.590592282131347 34.5 28.0 38.0 8.5 40.0 52-53 29.559822999983304 34.0 26.5 38.0 4.5 40.0 54-55 28.69017750096014 33.0 25.5 37.5 2.0 39.5 56-57 27.918229665870726 33.0 23.5 37.0 2.0 39.0 58-59 26.947410623340623 32.0 22.5 36.5 2.0 39.0 60-61 25.971756140731713 31.0 19.5 36.0 2.0 39.0 62-63 24.968890744234976 30.5 15.0 35.0 2.0 38.0 64-65 23.847738908277258 29.5 8.0 34.5 2.0 38.0 66-67 22.750988361413995 28.5 4.5 34.0 2.0 37.5 68-69 21.61146325579842 27.5 2.0 34.0 2.0 36.5 70-71 20.469244410306075 26.0 2.0 33.0 2.0 36.0 72-73 19.569400036735853 25.5 2.0 32.0 2.0 35.0 74-75 18.612233372852202 23.0 2.0 31.5 2.0 35.0 76-77 17.90368160034732 22.5 2.0 30.5 2.0 34.0 78-79 18.037520329954745 22.5 2.0 31.5 2.0 34.0 80-81 17.39363467864478 20.5 2.0 31.5 2.0 34.0 82-83 16.41924491124952 16.5 2.0 31.0 2.0 34.0 84-85 15.351009735000918 6.0 2.0 30.5 2.0 34.0 86-87 14.171466595421379 2.0 2.0 29.5 2.0 34.0 88-89 12.968515704576953 2.0 2.0 29.0 2.0 33.5 90-91 11.918899928198107 2.0 2.0 27.0 2.0 33.0 92-93 10.774090537178353 2.0 2.0 25.5 2.0 32.0 94-95 9.627313440312589 2.0 2.0 21.0 2.0 32.0 96-97 8.486842553475713 2.0 2.0 4.5 2.0 31.0 98-99 7.304524855143854 2.0 2.0 2.0 2.0 31.0 100-101 5.605327032578022 2.0 2.0 2.0 2.0 24.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3110202763421128 1101 2 0.32549126303210585 1101 3 0.31899748010391704 1101 4 0.27155432088661513 1101 5 0.20810967304431927 1101 6 0.3148001176985957 1101 7 0.34927821370848733 1101 8 0.4050533531915974 1101 9 0.5234297710679954 1101 10-11 0.42337992871883046 1101 12-13 0.3545035790813529 1101 14-15 0.6529383532226447 1101 16-17 0.49186446992009536 1101 18-19 0.5269135792308433 1101 20-21 0.7270352888517948 1101 22-23 0.5839162381950231 1101 24-25 0.526547589871555 1101 26-27 0.7557025560441133 1101 28-29 0.8511919501157408 1101 30-31 1.087612577838776 1101 32-33 0.8415985833772339 1101 34-35 0.7494396910907781 1101 36-37 0.8194801286096833 1101 38-39 1.2151491585958638 1101 40-41 1.3500711811584196 1101 42-43 1.5211274706217495 1101 44-45 1.6472190596255203 1101 46-47 1.6746918973102396 1101 48-49 1.684174268211958 1101 50-51 1.9152685420130773 1101 52-53 2.3651096648206043 1101 54-55 2.2389541678915243 1101 56-57 2.6736253702657358 1101 58-59 2.3531319451992836 1101 60-61 2.7458717315768126 1101 62-63 2.5411126527540837 1101 64-65 2.640959381960105 1101 66-67 3.0855344402625917 1101 68-69 3.552268149823597 1101 70-71 3.732064562868672 1101 72-73 3.662454911415061 1101 74-75 3.7025562048499907 1101 76-77 3.619457603199276 1101 78-79 3.8193275966724087 1101 80-81 3.8680586368942826 1101 82-83 3.995887121027902 1101 84-85 3.997251960151191 1101 86-87 3.509258766417812 1101 88-89 3.4388705989189905 1101 90-91 3.424943082012911 1101 92-93 3.5290257376520184 1101 94-95 3.1909674234255974 1101 96-97 2.4452155736144316 1101 98-99 1.8492084865058311 1101 100-101 1.1978783082865085 1102 1 0.28327414672613216 1102 2 0.2731589796730809 1102 3 0.2820914416346447 1102 4 0.3153968917598817 1102 5 0.3223697762545612 1102 6 0.3391567291857598 1102 7 0.22492327771224296 1102 8 0.3088098038316289 1102 9 0.3121101544203171 1102 10-11 0.3906676440194161 1102 12-13 0.35226679870470434 1102 14-15 0.6622942945134369 1102 16-17 0.5233804541034601 1102 18-19 0.40744856348123193 1102 20-21 0.36559084813066534 1102 22-23 0.45786486764345113 1102 24-25 0.5233155678891137 1102 26-27 0.5853629340348618 1102 28-29 0.4913358747856691 1102 30-31 0.6041329182985251 1102 32-33 0.5778260480243773 1102 34-35 0.7175651518507706 1102 36-37 0.7964773137849406 1102 38-39 0.8990250717384143 1102 40-41 1.2721633666687708 1102 42-43 1.2215790154243749 1102 44-45 1.2430998652015575 1102 46-47 1.0763187989924319 1102 48-49 1.364060837476753 1102 50-51 1.5812192155604272 1102 52-53 1.8431188465108406 1102 54-55 1.7871454754446354 1102 56-57 2.0654188157452573 1102 58-59 2.1324689534427 1102 60-61 2.427498298160824 1102 62-63 2.809881502026922 1102 64-65 2.7385005990367084 1102 66-67 2.6814286497659765 1102 68-69 3.0417988448172295 1102 70-71 3.357912361273602 1102 72-73 3.362581410979434 1102 74-75 3.124840234070554 1102 76-77 3.0970604783416924 1102 78-79 3.476167788348569 1102 80-81 3.6303416608202923 1102 82-83 3.7745799030130236 1102 84-85 3.5800781782330926 1102 86-87 3.3310661184719628 1102 88-89 2.6782731188573354 1102 90-91 2.7534440202878248 1102 92-93 2.9852225408153448 1102 94-95 2.3667875762303705 1102 96-97 1.8028197891495843 1102 98-99 1.4911468619498374 1102 100-101 1.1508983806677158 1103 1 0.24226464779944124 1103 2 0.3061794020372446 1103 3 0.265764710066307 1103 4 0.23816050831046454 1103 5 0.23201574922167367 1103 6 0.26344529436861563 1103 7 0.34255044976294613 1103 8 0.3370817475339152 1103 9 0.38348963227475963 1103 10-11 0.454351481402 1103 12-13 0.4336528083020994 1103 14-15 0.54630859213772 1103 16-17 0.5310060178355513 1103 18-19 0.5640136216104636 1103 20-21 0.5316738701941688 1103 22-23 0.6476531932196607 1103 24-25 0.5729267814803976 1103 26-27 0.7717081448558929 1103 28-29 0.7121596621462558 1103 30-31 0.7804318817535929 1103 32-33 0.8074591544425544 1103 34-35 1.1973750092027586 1103 36-37 1.2709130893555667 1103 38-39 1.0252626565033651 1103 40-41 1.205761306708034 1103 42-43 1.2153075574999619 1103 44-45 1.4373942728479676 1103 46-47 1.6468868965156247 1103 48-49 1.6877321693614995 1103 50-51 1.7033201391948403 1103 52-53 1.9916644669607741 1103 54-55 2.1508952867133715 1103 56-57 2.4385615359700274 1103 58-59 2.7064686777309355 1103 60-61 2.800993043225912 1103 62-63 2.711644252054821 1103 64-65 2.918101539082528 1103 66-67 3.032575151180641 1103 68-69 3.1484559709816153 1103 70-71 3.348824376398362 1103 72-73 3.2818892759857015 1103 74-75 3.143325394869592 1103 76-77 3.245447564527879 1103 78-79 3.357586456660755 1103 80-81 3.5250327323543686 1103 82-83 3.6357372031384045 1103 84-85 3.6306166367417543 1103 86-87 3.572360027211367 1103 88-89 3.346770424103079 1103 90-91 2.965875830860716 1103 92-93 3.0216245351304316 1103 94-95 2.5417163506915834 1103 96-97 2.109261105213733 1103 98-99 1.6189871854516396 1103 100-101 1.143536384251969 1104 1 0.1278683435777772 1104 2 0.13041517919632994 1104 3 0.21316199345447728 1104 4 0.14772885694487314 1104 5 0.21342053358773683 1104 6 0.17290115390040484 1104 7 0.20461452253316992 1104 8 0.2104810148208074 1104 9 0.1567761548019746 1104 10-11 0.25621343170737987 1104 12-13 0.21031688004968885 1104 14-15 0.3799461573622267 1104 16-17 0.4082570781731718 1104 18-19 0.45974740847940865 1104 20-21 0.31726804274733666 1104 22-23 0.46222353597907073 1104 24-25 0.2886811998733876 1104 26-27 0.5002243241123949 1104 28-29 0.503989629754507 1104 30-31 0.57119256374434 1104 32-33 0.8104720727933596 1104 34-35 0.8744024974489335 1104 36-37 0.8358694742625943 1104 38-39 0.7473800593345175 1104 40-41 0.8423815588370118 1104 42-43 0.6441754569449287 1104 44-45 1.0046039550918096 1104 46-47 1.4183135139938727 1104 48-49 1.2381921603112396 1104 50-51 1.421453126879836 1104 52-53 1.54855327403941 1104 54-55 1.7557869009064255 1104 56-57 2.0486429883066073 1104 58-59 2.2406097595181826 1104 60-61 2.3112503236995927 1104 62-63 2.434040640643314 1104 64-65 2.495617396211145 1104 66-67 2.5751094539655135 1104 68-69 2.70663840274036 1104 70-71 2.670053626632928 1104 72-73 2.788702855976961 1104 74-75 2.711276482562514 1104 76-77 3.0574763566250063 1104 78-79 2.9768958502677947 1104 80-81 3.232678348219423 1104 82-83 3.28730060992201 1104 84-85 3.29723760471048 1104 86-87 3.108802981175206 1104 88-89 2.9966532053450106 1104 90-91 2.7689141231738734 1104 92-93 2.621995813083336 1104 94-95 2.3909103279227644 1104 96-97 2.1644443601881793 1104 98-99 1.7871737332889088 1104 100-101 1.4189733021528204 1105 1 0.37046795076071604 1105 2 0.3915192757170942 1105 3 0.31137327291575545 1105 4 0.37127683674285095 1105 5 0.19105100496820882 1105 6 0.2114042403201566 1105 7 0.42294364711229804 1105 8 0.36019341661653925 1105 9 0.46015017275932735 1105 10-11 0.5118180893953692 1105 12-13 0.5077144670306097 1105 14-15 0.5333096534340527 1105 16-17 0.5874973980384937 1105 18-19 0.5817100908587562 1105 20-21 0.6735601302894239 1105 22-23 0.5997130140935454 1105 24-25 0.6163467442055932 1105 26-27 0.7474044587925306 1105 28-29 0.9669490854222929 1105 30-31 0.9670258970776757 1105 32-33 1.0499957821087378 1105 34-35 1.3152695008159299 1105 36-37 1.3313590926688832 1105 38-39 1.3056222029933267 1105 40-41 1.7202680627651858 1105 42-43 1.6795114558225919 1105 44-45 1.548322389435242 1105 46-47 1.9285898888535797 1105 48-49 1.9449366889307704 1105 50-51 2.0547373580807324 1105 52-53 1.729743650021451 1105 54-55 2.167343438504634 1105 56-57 2.329419503458123 1105 58-59 2.7700254441422025 1105 60-61 2.7929317210059885 1105 62-63 2.9958497034940414 1105 64-65 3.2061813692751144 1105 66-67 3.3786237637298235 1105 68-69 3.398799598026553 1105 70-71 3.363459911705881 1105 72-73 3.4947950276941313 1105 74-75 3.4700377059070746 1105 76-77 3.354235615884267 1105 78-79 3.585130979336256 1105 80-81 3.7522420406997803 1105 82-83 3.981352237306968 1105 84-85 4.005609518290724 1105 86-87 3.7339152146207724 1105 88-89 3.454383283908422 1105 90-91 3.402044033387117 1105 92-93 2.936035515664704 1105 94-95 2.5367787342078394 1105 96-97 2.3448455947560802 1105 98-99 1.7657055795290875 1105 100-101 1.2451166803795317 1106 1 0.03112971546659793 1106 2 0.07219440940693289 1106 3 0.06882648147190551 1106 4 -0.03359615790955672 1106 5 0.06164592475430197 1106 6 -0.02638319283492052 1106 7 -3.250694652479069E-4 1106 8 -0.05907797375778756 1106 9 0.00412744548660271 1106 10-11 0.17465899848627942 1106 12-13 0.16813559002526546 1106 14-15 0.20236379781908198 1106 16-17 0.018978013011754058 1106 18-19 0.1610483261528728 1106 20-21 -0.015430511421804738 1106 22-23 0.2272096718475538 1106 24-25 0.40241626292216637 1106 26-27 -0.12867842890800318 1106 28-29 0.28185884478058654 1106 30-31 0.515033918468049 1106 32-33 0.207987092269164 1106 34-35 0.35477484840951234 1106 36-37 0.26950748839080774 1106 38-39 0.4740513473783565 1106 40-41 0.5657927953855051 1106 42-43 0.623258113576604 1106 44-45 0.6245891006651902 1106 46-47 0.3744388193348698 1106 48-49 0.5412501916045684 1106 50-51 0.8477788019309997 1106 52-53 0.9809167783636958 1106 54-55 0.9469056042800332 1106 56-57 0.9810672307308472 1106 58-59 1.235857663393535 1106 60-61 1.4172465605781817 1106 62-63 1.5855722970769293 1106 64-65 2.0885209414676282 1106 66-67 2.076317613997201 1106 68-69 1.710828341342065 1106 70-71 1.4664010881764682 1106 72-73 2.153949439458838 1106 74-75 1.9020363690086803 1106 76-77 2.0057195731035193 1106 78-79 2.2736470221170038 1106 80-81 2.477128404336142 1106 82-83 2.640175766718734 1106 84-85 2.6916021653495488 1106 86-87 2.386642487348041 1106 88-89 2.0617228561009355 1106 90-91 2.044884942478026 1106 92-93 1.799808911386629 1106 94-95 1.5479886272559682 1106 96-97 1.4596717979646368 1106 98-99 1.1880217559996753 1106 100-101 0.8279843274383545 1107 1 -0.03520865474387236 1107 2 -0.049666087085238786 1107 3 0.012431446393655676 1107 4 0.03411566076286476 1107 5 -0.021256125974247198 1107 6 -0.07498545942963375 1107 7 -0.12733262478094787 1107 8 -0.021880186385963896 1107 9 0.1376366197985277 1107 10-11 0.11179202600921201 1107 12-13 0.02967026352769153 1107 14-15 0.08164941573597417 1107 16-17 0.022534408046837484 1107 18-19 -0.04460871648069542 1107 20-21 0.28158244054797166 1107 22-23 0.26670373552807547 1107 24-25 0.18975166497290274 1107 26-27 0.30486379991012313 1107 28-29 0.18848526140228472 1107 30-31 0.24447064269902796 1107 32-33 0.23257762114126024 1107 34-35 0.25300407129132196 1107 36-37 0.4259274829941475 1107 38-39 0.5033025616255173 1107 40-41 0.3699839718560938 1107 42-43 0.5573393332204262 1107 44-45 0.5955362134552615 1107 46-47 0.8519374701713502 1107 48-49 0.8562872936013299 1107 50-51 0.7067703022008338 1107 52-53 0.7367573072357949 1107 54-55 0.9133114326664327 1107 56-57 0.9381429188042425 1107 58-59 1.1691523207060008 1107 60-61 1.2178874132495245 1107 62-63 1.6585451788902041 1107 64-65 1.862447007306809 1107 66-67 1.9138041223620164 1107 68-69 2.3007986597446504 1107 70-71 2.059388810788448 1107 72-73 2.2614521377858736 1107 74-75 2.0816619761322634 1107 76-77 2.013279610880094 1107 78-79 2.2280365256248302 1107 80-81 2.591165101583851 1107 82-83 2.661914163912474 1107 84-85 2.532301032052194 1107 86-87 2.577227349733363 1107 88-89 2.53272461001351 1107 90-91 2.8979468690835315 1107 92-93 2.545942208742268 1107 94-95 2.120025189587322 1107 96-97 2.1228011827460715 1107 98-99 1.7507686529235835 1107 100-101 1.2436831941409991 1108 1 -0.03589036813009727 1108 2 -0.015304486165160114 1108 3 0.014497124079035473 1108 4 0.09646229181348787 1108 5 -0.01647518850902685 1108 6 -0.09071322987573183 1108 7 0.057989403637684234 1108 8 -0.11856721832910466 1108 9 0.007541656925077689 1108 10-11 0.0033793913227881944 1108 12-13 -0.05482393693976917 1108 14-15 0.0700597912944616 1108 16-17 0.05171359597977698 1108 18-19 -0.014430145998552746 1108 20-21 0.004883655839549306 1108 22-23 0.2817328988159815 1108 24-25 0.19944637132601173 1108 26-27 0.21305161492928448 1108 28-29 0.3629056725238158 1108 30-31 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0.2386155132799903 2315 86-87 0.1454578058877729 2315 88-89 0.20823976207592487 2315 90-91 -0.0045996674002832805 2315 92-93 0.05107443835618675 2315 94-95 0.04677783943332159 2315 96-97 0.1360678567460596 2315 98-99 0.06529174631505885 2315 100-101 -0.18824498748016794 2316 1 -0.32880919322040114 2316 2 -0.4037175635647863 2316 3 -0.34329690446783445 2316 4 -0.45658731818168974 2316 5 -0.3800012266318191 2316 6 -0.5453187229871901 2316 7 -0.46323893997017507 2316 8 -0.42021973337273266 2316 9 -0.7108135458587981 2316 10-11 -0.5903960762580169 2316 12-13 -0.4142094833305947 2316 14-15 -0.49924725415481674 2316 16-17 -0.6314727668281108 2316 18-19 -0.6795359752309409 2316 20-21 -0.6757635053589581 2316 22-23 -0.8234843162118679 2316 24-25 -0.6494926092026034 2316 26-27 -0.6613905129548385 2316 28-29 -0.7897584551685028 2316 30-31 -0.5007741529902745 2316 32-33 -1.0620855693682714 2316 34-35 -1.083356312788247 2316 36-37 -0.7973812513694583 2316 38-39 -0.7609113961769722 2316 40-41 -0.6254674443516208 2316 42-43 -0.7237065275119292 2316 44-45 -0.3928064193051881 2316 46-47 -0.7350317226191301 2316 48-49 -0.3067166709862583 2316 50-51 -0.16071173354361434 2316 52-53 -0.1227908163972593 2316 54-55 0.2986839631452156 2316 56-57 0.19547321027451758 2316 58-59 0.21619412842801822 2316 60-61 0.42749671099239706 2316 62-63 0.5728217995529796 2316 64-65 0.4861693887300902 2316 66-67 0.7430205343470888 2316 68-69 1.214384364852961 2316 70-71 1.089404050179521 2316 72-73 1.1639998860733591 2316 74-75 1.6675919759878965 2316 76-77 1.2890194296314945 2316 78-79 1.2326839083112944 2316 80-81 0.9405784436752498 2316 82-83 1.1153805614611763 2316 84-85 1.1316151241129138 2316 86-87 1.5245614487957226 2316 88-89 1.4171211066678815 2316 90-91 1.404373534138136 2316 92-93 1.5654457420433605 2316 94-95 1.4371233983949505 2316 96-97 1.086755150581844 2316 98-99 0.9350137030538717 2316 100-101 0.4158729746360228 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14.0 3 24.0 4 77.0 5 127.0 6 320.0 7 895.0 8 1902.0 9 3456.0 10 5498.0 11 8225.0 12 12100.0 13 16757.0 14 22780.0 15 29758.0 16 37291.0 17 44881.0 18 46999.0 19 48462.0 20 51907.0 21 55651.0 22 58825.0 23 60888.0 24 63201.0 25 65648.0 26 70021.0 27 76716.0 28 82092.0 29 86955.0 30 89234.0 31 89509.0 32 87756.0 33 84082.0 34 76109.0 35 61681.0 36 38983.0 37 16178.0 38 2155.0 39 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.756424088369 20.91635741747138 15.867807242276562 21.45941125188305 2 18.67482233238056 22.139265762970453 36.011935576742594 23.17397632790639 3 21.774920198323613 24.114834261653314 29.862358389178368 24.247887150844704 4 16.136256616627982 17.90672433082305 33.76211865680364 32.19490039574532 5 14.58784601090991 37.203565925335184 33.374210427532994 14.834377636221916 6 34.21096398216641 35.71159016147077 15.443552022976606 14.63389383338621 7 29.57336316729841 32.62470987025565 19.31985238866532 18.482074573780622 8 27.60086669952858 35.31493333440201 18.202627082757246 18.88157288331216 9 26.76138746917522 16.2145698690598 18.851140683902717 38.17290197786226 10-11 26.23788134319635 26.267503798821114 26.057508307312105 21.43710655067043 12-13 26.974602167415302 23.274834271210782 26.861388949187635 22.889174612186284 14-15 23.292300499273633 24.758061014911416 25.032277455875228 26.91736102993972 16-17 22.90460367024563 27.945363768430543 26.08843321588993 23.0615993454339 18-19 23.20259822666021 25.98517207407284 28.604304773991018 22.207924925275936 20-21 23.379898809424414 25.095429725983937 29.198423697964497 22.326247766627148 22-23 23.372551638919965 24.94164008883397 29.19421577303922 22.49159249920684 24-25 23.371617311616443 25.36818411042351 28.942520597537314 22.317677980422737 26-27 22.81662188935752 25.616717812106305 28.984968985423215 22.581691313112962 28-29 22.959594141352543 25.893893919300492 28.821447643105685 22.32506429624128 30-31 22.952894618197604 25.661963364336167 28.711840633192516 22.673301384273717 32-33 22.662804277526956 25.60852165764355 28.745599624893174 22.98307443993632 34-35 23.445449581087917 25.663738095198337 28.57416255158121 22.316649772132536 36-37 23.535635314741015 25.2985069393074 28.596685924245214 22.569171821706373 38-39 23.11461113536323 25.58203693974404 28.474411082658012 22.828940842234715 40-41 23.37639001241801 25.516944006722692 28.555749793422798 22.5509161874365 42-43 23.26293775144261 25.64676233091601 28.318040546091773 22.772259371549605 44-45 23.196104899733697 25.658762606342794 28.45515150988904 22.68998098403447 46-47 23.66414202218913 26.13132270152878 27.660603356522568 22.543931919759522 48-49 23.51515353916543 26.299363801826775 27.327633710154124 22.857848948853672 50-51 23.550687127423313 26.346786447810043 27.384006545660995 22.71851987910565 52-53 23.991016414246833 26.086963781789034 27.258403326264464 22.66361647769967 54-55 23.65227845776212 26.377778148846996 27.30298729273465 22.666956100656236 56-57 23.582747507806367 26.45726117521332 27.14321973049243 22.816771586487885 58-59 23.544508824953663 26.158832467814385 27.105014443869287 23.191644263362665 60-61 23.225073889157912 26.34444871174044 27.39589560338638 23.034581795715265 62-63 23.459281647102042 26.331925125653317 27.411959189807472 22.796834037437172 64-65 23.284601522601918 26.341226871425764 27.23110339880335 23.143068207168973 66-67 23.095871672384128 26.45474656150813 27.14191059386789 23.307471172239854 68-69 23.743497278015766 26.047757706833895 27.099915406587733 23.108829608562605 70-71 23.551604556317436 26.246815667384016 26.743518121474192 23.458061654824355 72-73 23.527239039495154 26.424870639359167 26.536815099945166 23.511075221200517 74-75 23.482525422879757 26.656603269490876 26.353265316345787 23.507605991283583 76-77 23.4381084375574 26.323642860721026 27.006495566650525 23.231753135071052 78-79 23.445722777898375 26.518209294170685 26.856212533604957 23.17985539432598 80-81 23.66174539499083 26.411993529142237 26.65999409554267 23.266266980324264 82-83 23.63146770169264 26.475417966210035 26.611308540400312 23.281805791697018 84-85 23.529838082384575 26.496028791885053 26.49612898636253 23.478004139367847 86-87 23.826100141305158 26.348919872194145 26.195190815398554 23.629789171102146 88-89 23.441314475595707 26.705595538263733 25.811979227545212 24.041110758595355 90-91 23.44625985244666 26.45456861971735 26.1830533742866 23.916118153549384 92-93 23.617902157501664 26.1354828087497 26.136818661055194 24.10979637269344 94-95 23.44048739809555 25.8749397615308 26.2397268989019 24.44484594147175 96-97 23.60268505685708 25.66673902516406 26.098819443284853 24.631756474694008 98-99 23.37098201613038 25.99843037721041 25.63758411675322 24.99300348990599 100-101 23.99103243844737 25.359610840156186 25.94180769903832 24.707549022358123 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 579.0 1 423.0 2 336.5 3 441.0 4 414.0 5 408.0 6 691.0 7 1528.0 8 2319.0 9 2525.5 10 2090.5 11 1527.0 12 1476.5 13 1567.5 14 1704.5 15 1884.0 16 2041.0 17 2236.0 18 2462.0 19 2680.5 20 3077.5 21 3577.0 22 4196.5 23 4974.5 24 5837.5 25 7032.5 26 8524.0 27 10144.0 28 11716.0 29 13326.5 30 15203.5 31 18162.5 32 21601.0 33 22627.5 34 22300.5 35 23203.5 36 25282.0 37 27785.5 38 30462.5 39 32599.0 40 34531.0 41 37381.0 42 39825.0 43 42253.5 44 44941.5 45 47493.5 46 49797.5 47 52159.5 48 53601.5 49 53825.0 50 53876.0 51 53554.5 52 53601.0 53 54201.5 54 55014.5 55 53975.0 56 51266.0 57 48569.5 58 45498.0 59 41206.0 60 35782.0 61 30721.0 62 26300.5 63 21459.0 64 16796.0 65 13150.5 66 10224.0 67 7964.0 68 6284.0 69 5033.0 70 3978.5 71 3050.0 72 2276.5 73 1747.0 74 1421.5 75 1097.5 76 809.5 77 621.5 78 450.0 79 297.5 80 177.0 81 109.0 82 72.5 83 43.0 84 25.0 85 14.5 86 9.0 87 5.5 88 2.5 89 2.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0180339639654683 2 0.009217359360128241 3 0.001736603937415466 4 0.0 5 2.6716983652545626E-4 6 0.0 7 0.0 8 6.679245913136407E-5 9 2.0037737739409222E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.002003773773940922 26-27 0.009150566900996877 28-29 2.3377360695977422E-4 30-31 0.0 32-33 1.0018868869704611E-4 34-35 0.00951792542621938 36-37 0.001035283116536143 38-39 0.01132132182276621 40-41 0.010419623624492795 42-43 0.0042747173844073 44-45 8.015095095763689E-4 46-47 3.339622956568203E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 6.679245913136407E-5 66-67 4.6754721391954843E-4 68-69 9.016981982734149E-4 70-71 6.679245913136407E-5 72-73 2.6716983652545626E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.679245913136407E-5 82-83 9.684906574047791E-4 84-85 0.005777547714862992 86-87 0.004040943777447526 88-89 0.0 90-91 0.0014360378713243276 92-93 2.3377360695977422E-4 94-95 3.339622956568203E-5 96-97 0.0 98-99 0.0 100-101 6.679245913136407E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1497175.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.380722121644126 #Duplication Level Percentage of deduplicated Percentage of total 1 78.56254616851194 45.07975630864222 2 12.096530331653527 13.882152911933016 3 3.972935479734606 6.839097203096169 4 1.7763048600393265 4.077026223089702 5 0.9530104556710128 2.734221406793992 6 0.6043308778110005 2.0806165301521373 7 0.38478290487635075 1.5455384659308233 8 0.2720616967473806 1.2488877296803567 9 0.195823195572301 1.0112828733095918 >10 1.0707557908734688 10.894870855300256 >50 0.06743238384315314 2.72246607252487 >100 0.03823967782452115 4.248517720451537 >500 0.003497451227540094 1.4268125341970581 >1k 0.0015155621986007075 1.414102403319031 >5k 2.3316341516933963E-4 0.7946507615793921 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6274 0.4190558885901782 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5605 0.3743717334312956 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4166 0.2782573847412627 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1757 0.11735435069380666 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1747 0.11668642610249302 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1664 0.11114265199458982 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1595 0.1065339723145257 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1556 0.10392906640840249 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 6.679245913136407E-5 0.0 0.0 0.0 1.6698114782841018E-4 14-15 6.679245913136407E-5 0.0 0.0 0.0 2.003773773940922E-4 16-17 6.679245913136407E-5 0.0 0.0 0.0 2.003773773940922E-4 18-19 1.001886886970461E-4 0.0 0.0 0.0 2.003773773940922E-4 20-21 1.3358491826272813E-4 0.0 0.0 3.339622956568203E-5 3.005660660911383E-4 22-23 1.3358491826272813E-4 0.0 0.0 2.003773773940922E-4 3.3396229565682035E-4 24-25 1.3358491826272813E-4 0.0 0.0 4.007547547881844E-4 3.3396229565682035E-4 26-27 1.3358491826272813E-4 0.0 0.0 5.677359026165945E-4 3.3396229565682035E-4 28-29 2.3377360695977422E-4 0.0 0.0 0.0015362265600213736 3.3396229565682035E-4 30-31 2.6716983652545626E-4 0.0 0.0 0.004842453287023895 3.3396229565682035E-4 32-33 2.6716983652545626E-4 0.0 0.0 0.0124567936279994 3.3396229565682035E-4 34-35 4.0075475478818445E-4 0.0 0.0 0.024579624960341977 3.3396229565682035E-4 36-37 5.343396730509125E-4 0.0 0.0 0.04698849499891462 3.3396229565682035E-4 38-39 5.343396730509125E-4 0.0 0.0 0.08005076226893984 3.3396229565682035E-4 40-41 5.343396730509125E-4 0.0 0.0 0.11732095446424098 3.3396229565682035E-4 42-43 5.343396730509125E-4 0.0 0.0 0.1565281279743517 3.3396229565682035E-4 44-45 6.679245913136407E-4 0.0 0.0 0.20341643428456926 3.3396229565682035E-4 46-47 8.015095095763689E-4 0.0 0.0 0.26463172307846444 3.6735852522250235E-4 48-49 8.683019687077329E-4 0.0 0.0 0.33242606909679895 4.675472139195485E-4 50-51 8.683019687077329E-4 0.0 0.0 0.4030924908577822 5.343396730509125E-4 52-53 8.683019687077329E-4 0.0 0.0 0.47536193163791807 5.343396730509125E-4 54-55 8.683019687077329E-4 0.0 0.0 0.5614574114582463 5.343396730509125E-4 56-57 8.683019687077329E-4 0.0 0.0 0.6591413829378663 5.343396730509125E-4 58-59 8.683019687077329E-4 0.0 0.0 0.7639387513149766 5.343396730509125E-4 60-61 8.683019687077329E-4 0.0 0.0 0.8769849883948102 5.343396730509125E-4 62-63 8.683019687077329E-4 0.0 0.0 0.9905321689181291 5.343396730509125E-4 64-65 9.35094427839097E-4 0.0 0.0 1.1120944445372118 5.343396730509125E-4 66-67 9.35094427839097E-4 0.0 0.0 1.2602067226610116 5.343396730509125E-4 68-69 9.35094427839097E-4 0.0 0.0 1.4169018317831918 5.343396730509125E-4 70-71 9.684906574047791E-4 0.0 0.0 1.5874229799455641 5.343396730509125E-4 72-73 0.001001886886970461 0.0 0.0 1.762619600247132 5.677359026165945E-4 74-75 0.001001886886970461 0.0 0.0 1.9451967872827158 6.679245913136407E-4 76-77 0.001001886886970461 3.339622956568203E-5 0.0 2.139796616961945 7.347170504450048E-4 78-79 0.0011688680347988712 6.679245913136407E-5 0.0 2.3614139963598113 7.347170504450048E-4 80-81 0.0013358491826272814 6.679245913136407E-5 0.0 2.5883413762586205 7.347170504450048E-4 82-83 0.0013358491826272814 6.679245913136407E-5 0.0 2.82578856847062 8.015095095763688E-4 84-85 0.0013358491826272814 6.679245913136407E-5 0.0 3.059328401823434 8.015095095763688E-4 86-87 0.0013358491826272814 6.679245913136407E-5 0.0 3.2963414430510793 8.349057391420509E-4 88-89 0.0013358491826272814 6.679245913136407E-5 0.0 3.543005994623207 8.683019687077329E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2890 0.0 63.291008 1 GTATCAA 5065 0.0 40.615105 1 TGGTATC 1055 0.0 35.11836 2 GTGGTAT 1160 0.0 33.174637 1 GTAAGGT 910 0.0 31.838423 4 AGGTAAG 840 0.0 29.40466 2 TATCAAC 7090 0.0 29.076063 2 ATCAACG 7155 0.0 28.610847 3 GGTAAGG 920 0.0 28.394743 3 TCAACGC 7265 0.0 28.177647 4 TAAGGTG 1065 0.0 27.650639 5 CAACGCA 7460 0.0 27.4411 5 AACGCAG 7750 0.0 26.414272 6 AAGGTAA 920 0.0 24.787489 1 ACGCAGA 8795 0.0 22.897764 7 CGCAGAG 8895 0.0 22.586945 8 GAGTACA 4295 0.0 20.574482 1 AGAGTAC 8970 0.0 20.54482 10-11 AAGGTGT 1515 0.0 20.378105 6 AGGTGTG 1620 0.0 20.230059 7 >>END_MODULE