FastQCFastQC Report
Thu 26 May 2016
SRR938303_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938303_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939680
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100560.5184360306854738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76860.3962509279881217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55780.2875732079518271No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19400.10001649756660891No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49450.049.2480741
GTATCAA88350.040.216071
TATCAAC113300.031.1904772
ATCAACG112700.031.1455213
TCAACGC115000.030.6466854
CAACGCA115950.030.272535
AACGCAG119900.029.489636
GTACATG94150.027.7356011
TACATGG94950.026.4986552
ACGCAGA136450.025.632227
CGCAGAG137550.025.426588
ACATGGG96150.024.6344683
GCAGAGT144400.023.4958179
GAGTACT81900.022.77039112-13
CATGGGG44100.022.756584
GTGTAAG9050.022.5998421
TGGTATC25100.022.549472
AGTACTT85300.021.52857212-13
GTGGTAT27750.021.082771
AGAGTAC131850.021.02908110-11
Produced by FastQC (version 0.11.5)