##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938303_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1939680 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.12015383980863 31.0 31.0 34.0 28.0 34.0 2 31.155977790150953 31.0 31.0 34.0 28.0 34.0 3 31.194385156314443 31.0 31.0 34.0 27.0 34.0 4 34.85415687123649 37.0 35.0 37.0 32.0 37.0 5 34.8416496535511 37.0 35.0 37.0 32.0 37.0 6 34.859077270477606 37.0 35.0 37.0 32.0 37.0 7 34.769573331683574 37.0 35.0 37.0 32.0 37.0 8 34.74309834611895 37.0 35.0 37.0 32.0 37.0 9 36.22153860430586 39.0 35.0 39.0 32.0 39.0 10-11 36.04779757485771 39.0 35.0 39.0 30.0 39.0 12-13 35.897996576754934 38.0 35.0 39.0 30.0 39.0 14-15 36.831344603233525 39.0 36.0 41.0 30.5 41.0 16-17 36.659871731419614 39.0 36.0 40.5 30.0 41.0 18-19 36.83835349129753 39.0 36.0 41.0 30.0 41.0 20-21 36.83827847892436 39.0 36.0 41.0 30.0 41.0 22-23 36.7415607213561 39.0 36.0 41.0 30.0 41.0 24-25 36.59332389878743 39.0 36.0 41.0 30.0 41.0 26-27 36.378302348841046 39.0 35.5 41.0 29.5 41.0 28-29 36.16263481605213 39.0 35.0 40.5 29.0 41.0 30-31 35.88315804668811 38.5 35.0 40.0 27.5 41.0 32-33 35.592360595562155 38.0 34.5 40.0 27.0 41.0 34-35 35.321018673183204 38.0 34.0 40.0 26.5 41.0 36-37 35.026135238802276 38.0 34.0 40.0 25.0 41.0 38-39 34.86102140559268 38.0 34.0 40.0 25.0 41.0 40-41 34.74317567846243 38.0 33.5 40.0 24.5 41.0 42-43 34.42592437927905 38.0 33.0 40.0 24.0 41.0 44-45 34.042583828260334 38.0 33.0 40.0 23.0 41.0 46-47 33.594631588715664 38.0 33.0 40.0 20.0 41.0 48-49 33.18906701105337 37.5 32.0 40.0 17.5 41.0 50-51 32.49022828507795 36.5 30.5 39.5 15.0 40.5 52-53 32.513430823641016 37.0 31.0 39.0 14.5 40.0 54-55 32.606754206879486 37.0 31.0 40.0 12.5 41.0 56-57 32.284792079105834 37.0 31.0 40.0 8.5 41.0 58-59 31.907997195413675 36.0 31.0 39.0 7.5 41.0 60-61 31.421848964777695 36.0 30.0 39.0 4.5 40.5 62-63 30.838531613462017 35.0 29.0 39.0 2.0 40.0 64-65 30.32129423410047 35.0 28.0 38.0 2.0 40.0 66-67 29.776575002062195 34.5 28.0 38.0 2.0 40.0 68-69 29.187273158459128 34.0 27.0 37.5 2.0 40.0 70-71 28.55268549451456 34.0 26.0 37.0 2.0 39.0 72-73 27.902788346531388 33.5 25.5 36.0 2.0 39.0 74-75 27.2176090901592 33.0 24.5 36.0 2.0 38.5 76-77 26.466302431328877 32.0 22.5 35.0 2.0 37.5 78-79 25.67614941639858 31.5 20.0 35.0 2.0 37.0 80-81 24.96531128845995 31.0 17.5 35.0 2.0 36.0 82-83 24.229667522477932 31.0 10.5 34.0 2.0 36.0 84-85 23.511098480161678 30.0 6.0 34.0 2.0 35.0 86-87 22.738145467293574 29.5 2.0 34.0 2.0 35.0 88-89 21.921165346861336 29.0 2.0 34.0 2.0 35.0 90-91 21.004175946547885 28.5 2.0 34.0 2.0 35.0 92-93 20.060289326074404 26.5 2.0 33.5 2.0 35.0 94-95 19.051809576837414 25.5 2.0 33.0 2.0 35.0 96-97 17.803433556050486 24.0 2.0 32.5 2.0 34.0 98-99 16.305207817784378 10.0 2.0 32.0 2.0 34.0 100-101 12.84642028582034 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5999375939101768 1101 2 0.4477978478875002 1101 3 0.4893390098469439 1101 4 0.4741714528024019 1101 5 0.21154527991073735 1101 6 0.5000903031742681 1101 7 0.5317827430892379 1101 8 0.4095796203662516 1101 9 0.6136879453847541 1101 10-11 0.6452212541550679 1101 12-13 0.754821764356123 1101 14-15 0.8802380416329569 1101 16-17 0.7226599208563584 1101 18-19 0.8209347500375159 1101 20-21 0.8088178786638025 1101 22-23 0.7829647268414774 1101 24-25 0.7312538611473869 1101 26-27 0.7616237445373528 1101 28-29 0.8435060674638493 1101 30-31 0.6541106201180114 1101 32-33 0.6814918140276802 1101 34-35 0.6770120595254099 1101 36-37 0.7185389930290143 1101 38-39 0.7305222676913843 1101 40-41 0.6724242378720149 1101 42-43 0.769808436598602 1101 44-45 0.5813289415615515 1101 46-47 0.6997607443763556 1101 48-49 0.608599332536123 1101 50-51 0.5467946242263082 1101 52-53 0.5475159262127818 1101 54-55 0.5209557096671418 1101 56-57 0.4633964382153053 1101 58-59 0.6506618902085997 1101 60-61 0.6140890683057165 1101 62-63 0.6445641954567272 1101 64-65 0.535972857892812 1101 66-67 0.5778539833014236 1101 68-69 0.44515834379066277 1101 70-71 0.690655538279227 1101 72-73 0.7933052713412891 1101 74-75 0.7539858654503284 1101 76-77 0.8613761005306095 1101 78-79 0.9652837874682838 1101 80-81 0.9771172697495416 1101 82-83 1.1391112466579045 1101 84-85 1.2409593466751154 1101 86-87 1.1448558472400059 1101 88-89 1.1964274432601094 1101 90-91 1.2007940932859427 1101 92-93 1.1177914124659871 1101 94-95 1.0959712599254843 1101 96-97 1.2254804031198034 1101 98-99 1.1564132343303157 1101 100-101 1.1546372498301753 1102 1 0.44741712219290264 1102 2 0.3660461094020988 1102 3 0.31072518715602726 1102 4 0.20704678952191102 1102 5 0.27386763528597413 1102 6 0.21526438597877018 1102 7 0.36447989594710606 1102 8 0.4209828732716332 1102 9 0.6226377062207007 1102 10-11 0.6251363589869996 1102 12-13 0.5475009702680467 1102 14-15 0.6499424712603386 1102 16-17 0.750528253924486 1102 18-19 0.6339306872626267 1102 20-21 0.6566863717174343 1102 22-23 0.6863987586734552 1102 24-25 0.7748266289852737 1102 26-27 0.7243575483614109 1102 28-29 0.5952111273198568 1102 30-31 0.756956590925796 1102 32-33 0.7122495996796587 1102 34-35 0.41424928689652774 1102 36-37 0.645739015198437 1102 38-39 0.7080690156002163 1102 40-41 0.7249959049524506 1102 42-43 0.6065260824575347 1102 44-45 0.647222687756539 1102 46-47 0.49015401447160656 1102 48-49 0.6514581650361535 1102 50-51 0.5535168725255346 1102 52-53 0.44073988278585574 1102 54-55 0.44219512883974943 1102 56-57 0.5719193234282756 1102 58-59 0.5386407504089838 1102 60-61 0.4862485041510993 1102 62-63 0.4420352530780782 1102 64-65 0.6614632063384001 1102 66-67 0.5123613436347867 1102 68-69 0.5491057862651623 1102 70-71 0.6069826880773732 1102 72-73 0.7964024193995947 1102 74-75 0.5711777083605547 1102 76-77 0.7489358558327694 1102 78-79 0.7687381603150847 1102 80-81 1.082965825751753 1102 82-83 1.225741114752708 1102 84-85 1.2465052729440806 1102 86-87 1.341206063520417 1102 88-89 1.1849650528537552 1102 90-91 1.0972778974671087 1102 92-93 1.1815237829912881 1102 94-95 1.1312072114121676 1102 96-97 1.3775027770933619 1102 98-99 1.4564936270490314 1102 100-101 1.0932656654851005 1103 1 0.36653329152282765 1103 2 0.2718680522981032 1103 3 0.24441743041679231 1103 4 0.2700068703526597 1103 5 0.21942270400179353 1103 6 0.38497220381101727 1103 7 0.2952266819985496 1103 8 0.36811640849755634 1103 9 0.4764407462200353 1103 10-11 0.5018313099674643 1103 12-13 0.4684858670250023 1103 14-15 0.7141986361948867 1103 16-17 0.7649558801292358 1103 18-19 0.6658935283364897 1103 20-21 0.4626569005589971 1103 22-23 0.6837101132658674 1103 24-25 0.8696373166686797 1103 26-27 0.7864635709989969 1103 28-29 0.9351247677861068 1103 30-31 0.931599950101571 1103 32-33 0.9456967797948508 1103 34-35 0.8992596602688181 1103 36-37 1.0435145108189516 1103 38-39 0.9477447803340624 1103 40-41 0.8909923914131639 1103 42-43 1.0211965602961186 1103 44-45 0.9655085726445876 1103 46-47 0.9589611941445 1103 48-49 0.9176585252361278 1103 50-51 1.2550234649038359 1103 52-53 1.0762743153326753 1103 54-55 0.9567218584147028 1103 56-57 0.9895783829552585 1103 58-59 1.0218732065553944 1103 60-61 1.1891770985833432 1103 62-63 1.244871931553302 1103 64-65 1.3232832528630745 1103 66-67 1.3904393187746287 1103 68-69 1.2832801390869797 1103 70-71 1.3153350150715006 1103 72-73 1.5069799418706253 1103 74-75 1.5297165625486748 1103 76-77 1.5284811690173896 1103 78-79 1.6352137155082005 1103 80-81 1.553209105865978 1103 82-83 1.5588083295057018 1103 84-85 1.7885736169502593 1103 86-87 1.7406513887301323 1103 88-89 1.6119416260333708 1103 90-91 1.752279300394875 1103 92-93 1.6510552327616317 1103 94-95 1.6624749466997386 1103 96-97 1.8417489902250423 1103 98-99 1.5483165623609345 1103 100-101 1.6395267593264542 1104 1 0.3358913026107828 1104 2 0.28170082803980634 1104 3 0.3186548340359465 1104 4 0.10968743289474503 1104 5 0.3120129899305226 1104 6 0.4453142643183554 1104 7 0.2777502432391472 1104 8 0.34610897216813896 1104 9 0.43982573995211993 1104 10-11 0.492061213380353 1104 12-13 0.5524622272691246 1104 14-15 0.5796294189790032 1104 16-17 0.8044941836134569 1104 18-19 0.6838747272516414 1104 20-21 0.7217818410071501 1104 22-23 0.7726361305711649 1104 24-25 0.6698819117560149 1104 26-27 0.5652305083437952 1104 28-29 0.7058464164474856 1104 30-31 0.736829334515086 1104 32-33 0.6009767333776779 1104 34-35 0.5949229547703538 1104 36-37 0.7206826639530419 1104 38-39 0.8256682771655122 1104 40-41 0.8694320930018336 1104 42-43 0.9102263774752615 1104 44-45 0.6969222736956269 1104 46-47 0.6380290180466872 1104 48-49 0.4524261599031618 1104 50-51 0.5272017717948145 1104 52-53 0.6957590154974227 1104 54-55 0.7607920111602127 1104 56-57 0.8664215626381662 1104 58-59 0.8156046257125915 1104 60-61 0.9917649092843135 1104 62-63 1.0206060495072151 1104 64-65 1.0802104305977345 1104 66-67 1.0959477651566694 1104 68-69 1.1038949271090068 1104 70-71 1.2995180435259748 1104 72-73 1.387267015060182 1104 74-75 1.1130882219892726 1104 76-77 1.0351633524344308 1104 78-79 1.1707014285308261 1104 80-81 1.1595391681725147 1104 82-83 1.0986363410993505 1104 84-85 0.9948869115848709 1104 86-87 1.2235270710812252 1104 88-89 1.169893894060614 1104 90-91 1.213641128124344 1104 92-93 1.2740245479220071 1104 94-95 1.3095588356285504 1104 96-97 1.4678501338867278 1104 98-99 1.7018650542019955 1104 100-101 1.2796447451830577 1105 1 0.30832618959102476 1105 2 0.25982506897652513 1105 3 0.12009353526152822 1105 4 0.208322211535382 1105 5 0.2201890733012064 1105 6 0.2765590620352967 1105 7 0.2751032403131646 1105 8 0.33819286320653674 1105 9 0.43377813353589545 1105 10-11 0.3845674357936417 1105 12-13 0.517384992104077 1105 14-15 0.5901107171842952 1105 16-17 0.5480507209331975 1105 18-19 0.5722107385856319 1105 20-21 0.5626938653825491 1105 22-23 0.576144819590759 1105 24-25 0.5282070383131412 1105 26-27 0.7101245723292138 1105 28-29 0.6689677557933607 1105 30-31 0.5281376216821272 1105 32-33 0.5620656887338313 1105 34-35 0.5121191236541236 1105 36-37 0.659114056828443 1105 38-39 0.7121347696350995 1105 40-41 0.657560571988931 1105 42-43 0.6936012782571126 1105 44-45 0.7168032525734702 1105 46-47 0.7080981175778547 1105 48-49 0.6774958539809646 1105 50-51 0.7132791157059941 1105 52-53 0.5274636975775877 1105 54-55 0.7305471189255996 1105 56-57 0.6872866940532987 1105 58-59 0.7379207558136223 1105 60-61 0.6683208683185242 1105 62-63 0.6642162484719911 1105 64-65 0.6157175687144765 1105 66-67 0.6144734826016851 1105 68-69 0.7321803054286882 1105 70-71 0.7158940429487011 1105 72-73 0.7325448919290665 1105 74-75 0.6802049147245235 1105 76-77 0.6616884661859359 1105 78-79 0.917717769924586 1105 80-81 0.8382357491736521 1105 82-83 0.9150266198045216 1105 84-85 1.0731622791620588 1105 86-87 1.2938332270272497 1105 88-89 1.5493571861415596 1105 90-91 1.4277737049097894 1105 92-93 1.4800966984429174 1105 94-95 1.6790385717514766 1105 96-97 1.444746915509242 1105 98-99 1.6933002796164764 1105 100-101 1.4608653545069696 1106 1 0.15635317462577092 1106 2 0.026150964386744135 1106 3 -0.0605619601810794 1106 4 0.02102673755123874 1106 5 0.25145011251857596 1106 6 -0.039182545629017795 1106 7 0.2379431469890747 1106 8 0.15793364087353723 1106 9 0.06454825970561018 1106 10-11 0.07901252691620897 1106 12-13 0.09280694958189173 1106 14-15 0.3307623662978685 1106 16-17 0.08539629841395424 1106 18-19 -0.08576052528771783 1106 20-21 0.17183901875491614 1106 22-23 0.09237202629551433 1106 24-25 -0.017792273396402436 1106 26-27 0.08197323952558833 1106 28-29 0.18965088407349384 1106 30-31 -0.1022233259318881 1106 32-33 0.03216680669973471 1106 34-35 -0.28335157086131346 1106 36-37 -0.06531240996407206 1106 38-39 -0.0664173911414565 1106 40-41 0.27845246483829555 1106 42-43 -0.1192429302341651 1106 44-45 0.07716050568722466 1106 46-47 -0.011319517840760795 1106 48-49 0.34095775254922245 1106 50-51 -0.05992133370718733 1106 52-53 0.0632393654976866 1106 54-55 0.2979704833500918 1106 56-57 -0.3987232962802665 1106 58-59 0.05885946253609475 1106 60-61 0.5781904980081372 1106 62-63 0.4110612464729151 1106 64-65 0.38060058464131785 1106 66-67 0.06753637514233901 1106 68-69 0.22303110328891407 1106 70-71 0.049379626806267396 1106 72-73 0.46628200546156506 1106 74-75 0.8023716632100282 1106 76-77 0.6772777389767022 1106 78-79 0.9218103146255068 1106 80-81 0.7839475983975532 1106 82-83 0.6202495297548722 1106 84-85 0.6058947922825944 1106 86-87 0.6085325301331608 1106 88-89 0.6020769456447717 1106 90-91 0.7177297251738679 1106 92-93 0.8634228620839757 1106 94-95 0.54270847574508 1106 96-97 0.7854694180523722 1106 98-99 0.9714607221467553 1106 100-101 0.5009441030329658 1107 1 0.10561862412572864 1107 2 0.03878366959430579 1107 3 0.06320356993133913 1107 4 0.08028764343985983 1107 5 0.03868354177259903 1107 6 0.3631913691562829 1107 7 0.18627300340118325 1107 8 0.02309244741829275 1107 9 0.17909367871939708 1107 10-11 0.4357639425198485 1107 12-13 0.3707716559815495 1107 14-15 0.46948150760417917 1107 16-17 0.4678574700794087 1107 18-19 0.45282035343694105 1107 20-21 0.35863616360818185 1107 22-23 0.4342125797366876 1107 24-25 0.43207483818590475 1107 26-27 0.34651352865561336 1107 28-29 0.3749391102574009 1107 30-31 0.49426954352967556 1107 32-33 0.4289095747850098 1107 34-35 0.4099317013205521 1107 36-37 0.5418090901116486 1107 38-39 0.5165118213481037 1107 40-41 0.5395076086460406 1107 42-43 0.5654040604783077 1107 44-45 0.49697284965326105 1107 46-47 0.550256675590056 1107 48-49 0.5393630915368277 1107 50-51 0.5373454153633261 1107 52-53 0.5667052126857755 1107 54-55 0.6320639351756014 1107 56-57 0.6099909105664167 1107 58-59 0.5918675775236615 1107 60-61 0.5025911565874992 1107 62-63 0.6278498903759235 1107 64-65 0.726093371047611 1107 66-67 0.7679538499692669 1107 68-69 0.7086229697360515 1107 70-71 0.807841492227432 1107 72-73 0.7412435313332821 1107 74-75 0.744768442620142 1107 76-77 0.8792429730215439 1107 78-79 1.0206414339661478 1107 80-81 1.144008277594164 1107 82-83 1.0887136914097226 1107 84-85 1.3272690637169582 1107 86-87 1.332131949723454 1107 88-89 1.3401580271219835 1107 90-91 1.1816588252945408 1107 92-93 1.3365427596817234 1107 94-95 1.4348810059503236 1107 96-97 1.5643587249317932 1107 98-99 1.6187323568208605 1107 100-101 1.3759710959928118 1108 1 0.3713918457832257 1108 2 0.26867433005342534 1108 3 0.28439133375256276 1108 4 0.22317089594506712 1108 5 0.1503954675579564 1108 6 0.3181639636073399 1108 7 0.28199744073826594 1108 8 0.3654498180761294 1108 9 0.32329127653596146 1108 10-11 0.3807074378879065 1108 12-13 0.3672032575665227 1108 14-15 0.4550898216826411 1108 16-17 0.46712029171314384 1108 18-19 0.5837920970488213 1108 20-21 0.5016346339181581 1108 22-23 0.35018382750480015 1108 24-25 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2316 72-73 -2.4714152210238822 2316 74-75 -2.5976480381561764 2316 76-77 -2.549667284482517 2316 78-79 -2.997718360591392 2316 80-81 -3.1415164174219115 2316 82-83 -3.262573067955394 2316 84-85 -3.103149107712298 2316 86-87 -3.2191182157216467 2316 88-89 -3.6956381823289775 2316 90-91 -3.5070514315638768 2316 92-93 -3.46400642582109 2316 94-95 -3.4885305942531453 2316 96-97 -3.732662041137827 2316 98-99 -3.554038561550488 2316 100-101 -3.2430872343006367 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7787.0 3 2711.0 4 2973.0 5 3145.0 6 3655.0 7 4516.0 8 5624.0 9 7339.0 10 9080.0 11 11029.0 12 13739.0 13 16201.0 14 18716.0 15 22131.0 16 25068.0 17 29603.0 18 27299.0 19 25090.0 20 26386.0 21 28581.0 22 31463.0 23 34835.0 24 39073.0 25 43293.0 26 48647.0 27 54171.0 28 61853.0 29 70703.0 30 80353.0 31 92990.0 32 109171.0 33 129366.0 34 155232.0 35 187669.0 36 213406.0 37 203389.0 38 89125.0 39 4268.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.474314039087574 18.477403428232027 14.340344266842378 22.707938265838017 2 15.478353766318218 19.866693159444374 40.51975733303744 24.13519574119997 3 19.365690862038175 24.727558196789847 29.18918305136884 26.717567889803135 4 12.857099353738494 16.357987892338954 36.639120163555106 34.14579259036745 5 13.921365028829655 37.45645345922309 33.47787316810217 15.144308343845086 6 32.20341169562036 35.704016983139674 17.336404664377618 14.756166656862352 7 29.03633357707671 32.72590008859332 20.09325504667393 18.14451128765603 8 26.753642291214152 34.863889799934405 19.34013832765047 19.04232958120097 9 27.161242483027188 16.65511597615195 19.800255559027637 36.383385981793225 10-11 25.319061469526748 26.692845275754834 26.67427644755621 21.313816807162205 12-13 26.35270608273485 24.0146885643677 27.358818064726453 22.273787288171 14-15 23.098115739267698 25.2604852903486 26.058894299568525 25.582504670815183 16-17 22.299646357013103 28.574585407424312 26.564531787257252 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22.11467575630132 26.316611902116417 29.053842367313827 22.51486997426844 44-45 22.096181226803413 26.637446627067856 28.97687153606029 22.289500610068437 46-47 22.345989781549555 26.876334475075964 28.369863769462516 22.40781197391196 48-49 22.344624051340713 26.840622661301406 28.342416796789504 22.47233649056838 50-51 22.441252513201153 27.05584945876752 28.11140038829425 22.39149763973708 52-53 22.622032882398056 26.957869024677912 28.058417494312287 22.36168059861175 54-55 22.137315627640085 26.959735780966938 28.240458707010717 22.662489884382257 56-57 22.2811810593461 27.20398041759941 28.1008840645882 22.41395445846629 58-59 22.362464294563182 26.838731700601066 28.125867739765603 22.672936265070152 60-61 22.315582551782924 26.95177034252767 28.3393005676049 22.3933465380845 62-63 22.207023249015126 26.986707887794452 28.31780107695009 22.488467786240335 64-65 22.016327391342568 26.81163175176714 28.348676193341433 22.823364663548855 66-67 22.041354027963788 27.033613521202287 28.137032105379166 22.788000345454755 68-69 22.209521098903252 26.794635210504364 28.263986746833268 22.731856943759116 70-71 22.103243496774656 26.865438035413934 28.13015140306395 22.901167064747455 72-73 22.29887120238167 26.86578555578418 27.922773101313314 22.912570140520838 74-75 22.12785828236314 27.139323513948444 27.89492262870312 22.837895574985296 76-77 22.23279923432975 26.94452294122986 27.8706704152606 22.952007409179792 78-79 22.333511635850407 27.076556226517233 27.732932904548 22.856999233084355 80-81 22.201602192626076 27.2763808058602 27.652980626853484 22.869036374660237 82-83 22.3393433756774 27.261112803911345 27.549782505244675 22.849761315166578 84-85 22.36422557075954 27.024239444850746 27.546103254482336 23.065431729907377 86-87 22.36911340647385 26.992125212853857 27.553066835789615 23.085694544882678 88-89 22.297076017359778 26.988444866204393 27.51792837931577 23.196550737120063 90-91 22.349786168581932 27.030586208808284 27.504303059136603 23.115324563473184 92-93 22.436057104459618 26.70834551673884 27.569692947239275 23.285904431562265 94-95 22.256627731161135 26.588756112336238 27.68523076978623 23.4693853867164 96-97 22.419113886935445 26.538288659426932 27.47408495662581 23.568512497011813 98-99 22.315660770540998 26.56317119116659 27.193024072508887 23.928143965783526 100-101 22.86030911719275 26.00756128843199 27.483163028931628 23.648966565443633 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1302.0 1 883.5 2 756.0 3 992.5 4 767.5 5 702.5 6 1171.5 7 2414.0 8 3891.5 9 4304.0 10 3483.0 11 2642.5 12 2537.0 13 2663.0 14 2799.5 15 2941.0 16 3017.5 17 3201.5 18 3399.0 19 3697.0 20 4107.5 21 4515.0 22 5154.0 23 6235.0 24 7396.0 25 8850.5 26 10927.5 27 13568.5 28 15822.0 29 18129.0 30 21293.0 31 24822.0 32 29064.5 33 32306.0 34 35144.0 35 38039.5 36 41299.0 37 45033.0 38 47423.0 39 49289.5 40 52003.0 41 56060.0 42 59584.0 43 62689.5 44 65612.0 45 68368.5 46 69882.0 47 71307.5 48 74306.5 49 74443.0 50 72253.0 51 70118.5 52 67777.5 53 66698.0 54 66403.0 55 63027.5 56 57598.5 57 53001.5 58 48500.5 59 42593.5 60 35562.0 61 29670.5 62 24741.5 63 19182.5 64 14584.0 65 11473.5 66 8773.5 67 6876.5 68 5157.0 69 3720.5 70 2918.0 71 2253.5 72 1782.0 73 1483.5 74 1167.5 75 898.0 76 667.0 77 441.5 78 270.0 79 177.0 80 118.0 81 71.5 82 36.5 83 27.0 84 16.0 85 10.0 86 10.0 87 7.5 88 5.0 89 4.5 90 3.5 91 2.5 92 3.0 93 2.5 94 1.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007114575600098985 2 0.1054297616101625 3 0.11352388022766642 4 0.11491586241029449 5 0.15255093623690505 6 0.09063350655778274 7 0.024849459704693558 8 0.02660232615689186 9 0.020725068052462263 10-11 0.048822486183287966 12-13 0.05304998762682505 14-15 0.026344551678627405 16-17 0.029772952239544667 18-19 0.03554710055266848 20-21 0.055859729439907614 22-23 0.030546275674338035 24-25 0.04668295801369298 26-27 0.05464818939206467 28-29 0.05124556627897386 30-31 0.06462406170089911 32-33 0.04807494019632104 34-35 0.06083477687041162 36-37 0.051013569248535846 38-39 0.03271158129175947 40-41 0.035933762270065166 42-43 0.03204136764827188 44-45 0.007398127526189887 46-47 0.012734059226264126 48-49 0.008583890126206384 50-51 0.00884166460447084 52-53 0.009924317413181555 54-55 0.011780293656685639 56-57 0.014718922708900439 58-59 0.019307308422007754 60-61 0.02469479501773488 62-63 0.0114194093871154 64-65 0.009202548874041079 66-67 0.010697640847974924 68-69 0.011290522147983173 70-71 0.030133836509114904 72-73 0.027684978965602576 74-75 0.02869029943083395 76-77 0.023921471582941514 78-79 0.004768827847892436 80-81 0.004536830817454426 82-83 0.004794605295718882 84-85 0.004820382743545327 86-87 0.013533160108883939 88-89 0.03686175039181721 90-91 0.03363956941351151 92-93 0.029231625835189307 94-95 0.045136311144106246 96-97 0.04106347438752784 98-99 0.02562278313948693 100-101 0.0714550853749072 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1939680.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.24898509484936 #Duplication Level Percentage of deduplicated Percentage of total 1 79.71389750877242 46.432536278408335 2 11.7391326315918 13.67585123368101 3 3.647650908117634 6.374158901344734 4 1.5614118136068342 3.638026138308248 5 0.8693391139804022 2.5319060546307 6 0.5259263087195121 1.8380804230555203 7 0.3328671137397889 1.3572420082756143 8 0.24090963999311854 1.122619362333175 9 0.17492336866373623 0.917019782463133 >10 1.0631445578170295 11.496108277184849 >50 0.0841026925489589 3.3749522674936387 >100 0.04287833904790004 4.386308339063144 >500 0.0027510722192326366 1.0608879229010082 >1k 7.986983862288295E-4 0.5888307912958816 >5k 1.774885302730732E-4 0.6856510943506781 >10k+ 8.87442651365366E-5 0.5198211252103753 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10056 0.5184360306854738 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7686 0.3962509279881217 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5578 0.2875732079518271 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1940 0.10001649756660891 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.1554895652891196E-5 0.0 4 0.0 0.0 0.0 5.1554895652891196E-5 0.0 5 0.0 0.0 0.0 5.1554895652891196E-5 0.0 6 0.0 0.0 0.0 5.1554895652891196E-5 0.0 7 0.0 0.0 0.0 5.1554895652891196E-5 0.0 8 0.0 0.0 0.0 5.1554895652891196E-5 0.0 9 0.0 0.0 0.0 5.1554895652891196E-5 0.0 10-11 0.0 0.0 0.0 1.28887239132228E-4 0.0 12-13 0.0 0.0 0.0 1.804421347851192E-4 0.0 14-15 0.0 0.0 0.0 2.0621958261156479E-4 0.0 16-17 0.0 0.0 0.0 4.1243916522312957E-4 0.0 18-19 0.0 0.0 0.0 4.897715087024664E-4 0.0 20-21 0.0 0.0 0.0 6.4443619566114E-4 0.0 22-23 0.0 0.0 0.0 9.279881217520416E-4 0.0 24-25 0.0 0.0 0.0 0.0012630949434958344 0.0 26-27 0.0 0.0 0.0 0.0017528664521983008 0.0 28-29 0.0 0.0 0.0 0.003789284830487503 0.0 30-31 0.0 0.0 0.0 0.01025942423492535 0.0 32-33 0.0 0.0 0.0 0.023534809865544834 0.0 34-35 0.0 0.0 0.0 0.043074115317990595 0.0 36-37 0.0 0.0 0.0 0.07568258681844428 0.0 38-39 0.0 0.0 0.0 0.12896457147570733 0.0 40-41 0.0 0.0 0.0 0.18686071929390413 0.0 42-43 0.0 0.0 0.0 0.24400932112513402 0.0 44-45 0.0 0.0 0.0 0.3185319227913883 0.0 46-47 0.0 0.0 0.0 0.4099645302317908 0.0 48-49 0.0 0.0 0.0 0.5119658912810361 0.0 50-51 0.0 0.0 0.0 0.6226026973521406 0.0 52-53 0.0 0.0 0.0 0.7284191206796997 0.0 54-55 0.0 0.0 0.0 0.8481295883857132 0.0 56-57 0.0 0.0 0.0 0.9886166790398416 0.0 58-59 0.0 0.0 0.0 1.1449311226594077 0.0 60-61 0.0 0.0 0.0 1.3190835601748743 0.0 62-63 0.0 0.0 0.0 1.4934164398251257 0.0 64-65 0.0 5.1554895652891196E-5 0.0 1.6736008001319806 0.0 66-67 0.0 5.1554895652891196E-5 0.0 1.8857234182958014 0.0 68-69 0.0 5.1554895652891196E-5 0.0 2.1197310896642745 0.0 70-71 0.0 5.1554895652891196E-5 0.0 2.3773251257939454 0.0 72-73 0.0 5.1554895652891196E-5 0.0 2.6435546069454756 0.0 74-75 0.0 5.1554895652891196E-5 0.0 2.9067681267013112 0.0 76-77 0.0 5.1554895652891196E-5 0.0 3.1972799637053537 0.0 78-79 2.5777447826445598E-5 5.1554895652891196E-5 0.0 3.51836385383156 0.0 80-81 5.1554895652891196E-5 5.1554895652891196E-5 0.0 3.864090984079848 0.0 82-83 5.1554895652891196E-5 5.1554895652891196E-5 0.0 4.225980574115318 0.0 84-85 5.1554895652891196E-5 5.1554895652891196E-5 0.0 4.5797760455332845 0.0 86-87 5.1554895652891196E-5 5.1554895652891196E-5 0.0 4.951564175534108 0.0 88-89 5.1554895652891196E-5 5.1554895652891196E-5 0.0 5.354182133135362 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4945 0.0 49.248074 1 GTATCAA 8835 0.0 40.21607 1 TATCAAC 11330 0.0 31.190477 2 ATCAACG 11270 0.0 31.145521 3 TCAACGC 11500 0.0 30.646685 4 CAACGCA 11595 0.0 30.27253 5 AACGCAG 11990 0.0 29.48963 6 GTACATG 9415 0.0 27.735601 1 TACATGG 9495 0.0 26.498655 2 ACGCAGA 13645 0.0 25.63222 7 CGCAGAG 13755 0.0 25.42658 8 ACATGGG 9615 0.0 24.634468 3 GCAGAGT 14440 0.0 23.495817 9 GAGTACT 8190 0.0 22.770391 12-13 CATGGGG 4410 0.0 22.75658 4 GTGTAAG 905 0.0 22.599842 1 TGGTATC 2510 0.0 22.54947 2 AGTACTT 8530 0.0 21.528572 12-13 GTGGTAT 2775 0.0 21.08277 1 AGAGTAC 13185 0.0 21.029081 10-11 >>END_MODULE