FastQCFastQC Report
Thu 26 May 2016
SRR938303_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938303_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939680
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101930.52549905138992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82450.4250701146580879No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60950.31422708900437185No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20750.10697640847974924No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49850.049.2923051
GTATCAA87100.038.906761
ATCAACG112200.029.9772993
TATCAAC114200.029.5075972
TCAACGC114900.029.2728714
CAACGCA116450.028.9648275
AACGCAG120400.028.0008496
GTACATG98450.026.1177231
TACATGG97750.025.7699092
ACGCAGA136250.024.6041017
CGCAGAG137150.024.4080248
ACATGGG99650.024.0749933
TGGTATC24000.022.5760172
GAGTACT81900.022.4950612-13
GCAGAGT148600.022.303659
GTGGTAT26500.021.7016741
GTACTTT85600.021.4395214-15
CATGGGA54950.021.0947424
AGTACTT85200.020.81566612-13
AGAGTAC138300.020.44555510-11
Produced by FastQC (version 0.11.5)