##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938303_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1939680 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.764593128763508 31.0 31.0 34.0 30.0 34.0 2 31.993190629382166 31.0 31.0 34.0 30.0 34.0 3 32.047056731007174 33.0 31.0 34.0 30.0 34.0 4 35.726961148230636 37.0 35.0 37.0 33.0 37.0 5 35.628079373917345 37.0 35.0 37.0 33.0 37.0 6 35.634403612967084 37.0 35.0 37.0 33.0 37.0 7 35.57095139404438 37.0 35.0 37.0 33.0 37.0 8 35.552897385135694 37.0 35.0 37.0 33.0 37.0 9 37.12150664027056 39.0 37.0 39.0 33.0 39.0 10-11 37.020402849954635 39.0 37.0 39.0 33.0 39.0 12-13 36.90192737977398 39.0 37.0 39.0 33.0 39.0 14-15 37.90712205105997 40.0 37.0 41.0 32.5 41.0 16-17 37.82565010723418 40.0 37.0 41.0 32.5 41.0 18-19 38.09012182421843 40.0 37.0 41.0 33.0 41.0 20-21 38.07049307102203 40.0 37.0 41.0 33.0 41.0 22-23 37.95737930998928 40.0 37.0 41.0 33.0 41.0 24-25 37.8330562257692 40.0 37.0 41.0 32.0 41.0 26-27 37.62958400354698 40.0 37.0 41.0 32.0 41.0 28-29 37.502375649591684 39.5 36.5 41.0 32.0 41.0 30-31 37.230815392229644 39.0 36.0 41.0 31.0 41.0 32-33 37.008105718469025 39.0 35.5 40.5 31.0 41.0 34-35 36.87436123484286 39.0 35.0 41.0 30.5 41.0 36-37 36.81397292336881 39.0 35.0 41.0 30.0 41.0 38-39 36.647128650086614 39.0 35.0 41.0 30.0 41.0 40-41 36.456445908603484 39.0 35.0 40.0 30.0 41.0 42-43 36.22641569743463 39.0 35.0 40.0 30.0 41.0 44-45 35.882506135032585 38.0 35.0 40.0 29.0 41.0 46-47 35.44088303225274 38.0 34.5 40.0 27.5 41.0 48-49 35.04682731172152 38.0 34.0 40.0 26.0 41.0 50-51 34.65254449187495 38.0 33.5 40.0 24.5 41.0 52-53 34.15589272457312 38.0 33.0 40.0 24.0 41.0 54-55 33.803305442134786 37.0 33.0 40.0 23.0 41.0 56-57 33.47169223789491 37.0 32.0 40.0 22.0 41.0 58-59 32.986012641260416 36.5 31.5 39.0 20.5 41.0 60-61 32.57604682215623 36.0 31.0 39.0 19.5 40.0 62-63 32.08481708323022 35.5 31.0 39.0 18.0 40.0 64-65 31.54761635939949 35.0 30.0 38.0 14.0 40.0 66-67 31.03476269281531 35.0 29.0 38.0 10.5 40.0 68-69 30.530972892435862 34.0 29.0 37.5 8.0 39.5 70-71 29.945416254227503 34.0 28.0 37.0 7.0 39.0 72-73 29.434969685721356 34.0 28.0 36.0 4.0 39.0 74-75 28.822473036789575 33.5 26.5 36.0 2.0 38.5 76-77 27.633958178668646 31.5 26.0 34.5 2.0 36.5 78-79 28.316251134207704 33.0 27.0 35.0 2.0 37.0 80-81 27.912750041243918 33.0 26.0 35.0 2.0 37.0 82-83 27.4504186257527 33.0 26.0 35.0 2.0 36.0 84-85 26.8761290522148 32.0 25.0 35.0 2.0 36.0 86-87 26.236109306689762 32.0 24.0 34.5 2.0 35.0 88-89 25.46791223294564 31.0 21.0 34.0 2.0 35.0 90-91 24.825809411861748 31.0 19.0 34.0 2.0 35.0 92-93 23.95907933267343 31.0 13.0 34.0 2.0 35.0 94-95 23.105968510269733 30.0 4.0 34.0 2.0 35.0 96-97 22.008489802441638 29.5 2.0 34.0 2.0 35.0 98-99 20.62748056380434 29.0 2.0 34.0 2.0 35.0 100-101 16.790263084632517 21.0 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30711402427567336 1101 2 0.305492124087408 1101 3 0.3605787684636752 1101 4 0.2925178035267635 1101 5 0.1053798350155759 1101 6 0.20871987236080258 1101 7 0.23839900648987822 1101 8 0.2646861114541963 1101 9 0.35652977930675434 1101 10-11 0.33624236210636127 1101 12-13 0.3787909545288741 1101 14-15 0.5131145770863128 1101 16-17 0.5939132753389558 1101 18-19 0.5086431681485948 1101 20-21 0.34785163302313293 1101 22-23 0.4311193913693927 1101 24-25 0.48500864955601486 1101 26-27 0.5066368713283751 1101 28-29 0.3414357715813381 1101 30-31 0.4835595304335456 1101 32-33 0.47755305007969184 1101 34-35 0.5431981031999342 1101 36-37 0.6457177518373598 1101 38-39 0.6849515982379231 1101 40-41 0.6671659294927963 1101 42-43 0.6902885252682438 1101 44-45 0.7840941265331907 1101 46-47 0.8053576428140232 1101 48-49 0.8258573921247319 1101 50-51 1.00793399409379 1101 52-53 1.0140486322903968 1101 54-55 1.020090941977962 1101 56-57 0.9270657369063571 1101 58-59 1.0996859340145804 1101 60-61 1.163786924060851 1101 62-63 1.2318372591761175 1101 64-65 1.0902314568910434 1101 66-67 1.1229841768391182 1101 68-69 1.1657890364511871 1101 70-71 1.1477214836009608 1101 72-73 1.1652938996952393 1101 74-75 1.1638791989612365 1101 76-77 1.2146093179645767 1101 78-79 1.3208388960166637 1101 80-81 1.4315824917860205 1101 82-83 1.2240223858510895 1101 84-85 1.4127147655109695 1101 86-87 1.3708364810624332 1101 88-89 1.4437239611247819 1101 90-91 1.410081401840273 1101 92-93 1.6644387133843601 1101 94-95 1.695469662212851 1101 96-97 1.5502356489164448 1101 98-99 1.7001161925959067 1101 100-101 2.0030374387906456 1102 1 0.26294859583019203 1102 2 0.19925317487223282 1102 3 0.15646496962773426 1102 4 0.2603538595182613 1102 5 0.11887918378884166 1102 6 0.1229927796432051 1102 7 0.22554674196640434 1102 8 0.33709498185078957 1102 9 0.20883287071247736 1102 10-11 0.3611736754702619 1102 12-13 0.32193110440672257 1102 14-15 0.4286329429943123 1102 16-17 0.38345447974986513 1102 18-19 0.3234489414542736 1102 20-21 0.27160896700174675 1102 22-23 0.42645652797730804 1102 24-25 0.3760995136120471 1102 26-27 0.3361903089170326 1102 28-29 0.20490085202975905 1102 30-31 0.3802741708504058 1102 32-33 0.5267960186451077 1102 34-35 0.5080568574213373 1102 36-37 0.4061684481600025 1102 38-39 0.47937907437793825 1102 40-41 0.34036210001107037 1102 42-43 0.35142164549706933 1102 44-45 0.49050728854109593 1102 46-47 0.47214596512883134 1102 48-49 0.3834814587449813 1102 50-51 0.5385362224062575 1102 52-53 0.3242279674763324 1102 54-55 0.32825660240242627 1102 56-57 0.28918622485073087 1102 58-59 0.47607470108910377 1102 60-61 0.46835062500564817 1102 62-63 0.4982514153796842 1102 64-65 0.5834869248957908 1102 66-67 0.5568664509302934 1102 68-69 0.7311192622564491 1102 70-71 0.5802191688106184 1102 72-73 0.4677669328519478 1102 74-75 0.6984689232633521 1102 76-77 0.7010758294262551 1102 78-79 0.6752989349217131 1102 80-81 0.7415979846685197 1102 82-83 0.6758803175755581 1102 84-85 0.6852966488089685 1102 86-87 0.7308710150774935 1102 88-89 0.6288231964226156 1102 90-91 0.638987526628398 1102 92-93 0.9241786169416635 1102 94-95 1.067287129030852 1102 96-97 0.8838014373864596 1102 98-99 1.1086484769575797 1102 100-101 1.3216342973037456 1103 1 0.12947308072072872 1103 2 0.14392419176679994 1103 3 0.21692631862226364 1103 4 0.1127241163438839 1103 5 0.04081668923123516 1103 6 0.11286571447133298 1103 7 0.20580208864160454 1103 8 0.17156719766116169 1103 9 0.26395701254225656 1103 10-11 0.3185589498428527 1103 12-13 0.30695585260537683 1103 14-15 0.5063359286733942 1103 16-17 0.333636031042424 1103 18-19 0.4210513862805101 1103 20-21 0.2556788950032143 1103 22-23 0.31601777786845275 1103 24-25 0.4253013652002622 1103 26-27 0.39406999438477186 1103 28-29 0.3757018477629188 1103 30-31 0.3226721683265126 1103 32-33 0.36463222026311115 1103 34-35 0.2909110068393588 1103 36-37 0.47570708534353656 1103 38-39 0.38782013828095785 1103 40-41 0.5126168882795525 1103 42-43 0.6515532304460763 1103 44-45 0.5814207396466458 1103 46-47 0.5584170384169411 1103 48-49 0.6057502762911113 1103 50-51 0.6257911419832496 1103 52-53 0.439920163742471 1103 54-55 0.3837513106694743 1103 56-57 0.5051891385174585 1103 58-59 0.5779264275195715 1103 60-61 0.6752300851756345 1103 62-63 0.6106868841579782 1103 64-65 0.7971953951240103 1103 66-67 0.7683341068798839 1103 68-69 0.79152656743263 1103 70-71 0.5960716996784825 1103 72-73 0.7333826246335597 1103 74-75 0.5968220381085381 1103 76-77 0.5322186143000671 1103 78-79 0.8098822791974207 1103 80-81 0.6139932039329992 1103 82-83 0.7221468019767983 1103 84-85 0.7190426594491051 1103 86-87 0.6966926538906328 1103 88-89 0.7128050786126181 1103 90-91 0.9852341074239064 1103 92-93 1.1507197987459001 1103 94-95 1.1088801362307095 1103 96-97 1.1978793682960678 1103 98-99 1.6898969306271034 1103 100-101 1.8993737729266655 1104 1 0.21162322856901028 1104 2 0.19012365253976782 1104 3 0.2014973989127924 1104 4 0.10021703000634119 1104 5 0.10322117338578352 1104 6 -0.07091675293151667 1104 7 0.07038317630458835 1104 8 0.0993086454830987 1104 9 0.1865651573723568 1104 10-11 0.2561919933350012 1104 12-13 0.21696628582346733 1104 14-15 0.4103233076733517 1104 16-17 0.38719144287508556 1104 18-19 0.2601272480190744 1104 20-21 0.17737732709281318 1104 22-23 0.254109137726644 1104 24-25 0.22354075964776143 1104 26-27 0.2690941478169506 1104 28-29 0.28787397989217567 1104 30-31 0.24730600761768784 1104 32-33 0.389167848904421 1104 34-35 0.44836689854681566 1104 36-37 0.5264666874972477 1104 38-39 0.4967282966848785 1104 40-41 0.4204189126432425 1104 42-43 0.4480537108531877 1104 44-45 0.519613104766691 1104 46-47 0.46970075008751166 1104 48-49 0.7385365310585925 1104 50-51 0.8148986759910102 1104 52-53 0.8250158326261925 1104 54-55 0.9232809641642064 1104 56-57 0.7215281701977219 1104 58-59 0.7660527865660001 1104 60-61 0.8203798364551673 1104 62-63 0.8396204336079691 1104 64-65 0.7569730770865171 1104 66-67 0.6561885901028965 1104 68-69 0.8431740577379898 1104 70-71 0.9651796595318878 1104 72-73 0.7455691290530346 1104 74-75 0.8354028542949301 1104 76-77 0.7459398930080496 1104 78-79 0.9865311668087742 1104 80-81 0.950185957637192 1104 82-83 1.0086808975738215 1104 84-85 1.005702113355305 1104 86-87 0.9461347961071063 1104 88-89 0.8703594471234624 1104 90-91 1.0627317586190728 1104 92-93 1.460235319488575 1104 94-95 1.5024493348158359 1104 96-97 1.527179943453831 1104 98-99 1.916911779332132 1104 100-101 2.0801046571152852 1105 1 0.2571035053736601 1105 2 0.2020958907690087 1105 3 0.19511186641751976 1105 4 0.12989557845884292 1105 5 0.04175877160031405 1105 6 0.19065480151828496 1105 7 0.22721695631014427 1105 8 0.15342814224256074 1105 9 0.2577024082090418 1105 10-11 0.17237387582209607 1105 12-13 0.2932425538593222 1105 14-15 0.35852101831309113 1105 16-17 0.2526915277377171 1105 18-19 0.3059210592459465 1105 20-21 0.35596719459530135 1105 22-23 0.18233592525397313 1105 24-25 0.17183578283508183 1105 26-27 0.1037766446689048 1105 28-29 0.2583044316561498 1105 30-31 0.2792595862294647 1105 32-33 0.1973112068021905 1105 34-35 0.22801978494369024 1105 36-37 0.2242139147248423 1105 38-39 0.22052606749070947 1105 40-41 0.2646670042501782 1105 42-43 0.4182880130434512 1105 44-45 0.4073550776248709 1105 46-47 0.29963398809011466 1105 48-49 0.2889657734029143 1105 50-51 0.43032050428797675 1105 52-53 0.4231698923633971 1105 54-55 0.45311983694443825 1105 56-57 0.42485676748435 1105 58-59 0.5855997521930689 1105 60-61 0.37651799689739107 1105 62-63 0.4872407734040749 1105 64-65 0.5006767265865442 1105 66-67 0.43327349237873847 1105 68-69 0.6579936153749628 1105 70-71 0.8004879548294817 1105 72-73 0.6582919697053917 1105 74-75 0.6735725958348375 1105 76-77 0.6496665316446375 1105 78-79 1.1105043034640474 1105 80-81 0.9556387633383316 1105 82-83 0.8726407509393113 1105 84-85 0.7001551965115489 1105 86-87 0.668043483907109 1105 88-89 0.6775339989013602 1105 90-91 0.7449346402900616 1105 92-93 0.8395418443584113 1105 94-95 0.9949679563213465 1105 96-97 1.284009099207772 1105 98-99 1.6089767668364878 1105 100-101 1.6973421393678159 1106 1 -0.0801411059699717 1106 2 0.11476752054971939 1106 3 0.0421241291217882 1106 4 0.050151055682000845 1106 5 -0.06118063353453351 1106 6 -0.06199081493185332 1106 7 0.08908262832961356 1106 8 0.039831369110181924 1106 9 -0.16999217302188185 1106 10-11 0.04521929894359289 1106 12-13 -0.007859723066253821 1106 14-15 -0.10568180468997923 1106 16-17 0.06019035106469772 1106 18-19 0.08385704823329831 1106 20-21 0.13632991354504753 1106 22-23 0.1523115314303709 1106 24-25 0.15658458212249826 1106 26-27 0.08548760316397619 1106 28-29 0.08745252038489326 1106 30-31 0.22532620869709064 1106 32-33 0.1086773621432684 1106 34-35 0.16483978180590242 1106 36-37 0.11600807539005586 1106 38-39 0.1880931631580296 1106 40-41 0.09428247256604294 1106 42-43 0.1951040117478513 1106 44-45 -0.0035469877525997617 1106 46-47 -0.4456803227843622 1106 48-49 -0.1014809068608642 1106 50-51 0.34828918707211187 1106 52-53 0.6822052229199471 1106 54-55 0.6086853738363018 1106 56-57 0.5308846230040842 1106 58-59 0.40869341536598824 1106 60-61 0.3576537773732227 1106 62-63 0.236447974136901 1106 64-65 0.41032974722007154 1106 66-67 0.29117542111031014 1106 68-69 0.16551167034710446 1106 70-71 0.45709275970419583 1106 72-73 0.7886860666025193 1106 74-75 0.7228157293783681 1106 76-77 0.7000354755742713 1106 78-79 0.7992621416246912 1106 80-81 0.09028178857733948 1106 82-83 0.6829405621655305 1106 84-85 0.9186530025857103 1106 86-87 1.1564161830685116 1106 88-89 1.1690389257451947 1106 90-91 1.3455189280359612 1106 92-93 1.4748997300920728 1106 94-95 1.461931785645561 1106 96-97 1.4407654798121534 1106 98-99 1.0762364092291072 1106 100-101 1.2151926487533622 1107 1 0.10539213323406571 1107 2 0.19635661197401788 1107 3 0.16275945301288175 1107 4 0.0693534320395841 1107 5 0.09353030972895482 1107 6 0.0883563072033482 1107 7 0.12146587461777614 1107 8 0.10798602777748556 1107 9 0.17979896742455992 1107 10-11 0.11197398790164215 1107 12-13 0.22627574495015068 1107 14-15 0.2685109714565499 1107 16-17 0.2225927000628971 1107 18-19 0.18822722064625452 1107 20-21 0.21706964278489949 1107 22-23 0.2573099924652382 1107 24-25 0.21133998437867518 1107 26-27 0.32420918727208203 1107 28-29 0.23239323358530584 1107 30-31 0.19720833862482579 1107 32-33 0.2984745118821337 1107 34-35 0.3694679140885029 1107 36-37 0.24097570826626225 1107 38-39 0.18072127589821463 1107 40-41 0.16640668415175952 1107 42-43 0.2377514226222246 1107 44-45 0.12869970591517443 1107 46-47 0.24503263334779746 1107 48-49 0.2867328187538689 1107 50-51 0.27233368514962564 1107 52-53 0.30353601248233986 1107 54-55 0.05325062840066863 1107 56-57 0.2286345582698388 1107 58-59 0.1368562329912919 1107 60-61 0.17960992419538258 1107 62-63 0.19567370367965964 1107 64-65 0.12818736118898855 1107 66-67 0.2005335505104071 1107 68-69 0.30495168246340043 1107 70-71 0.4171756683971495 1107 72-73 0.4803551353576161 1107 74-75 0.5527747391642315 1107 76-77 0.5556079950311847 1107 78-79 0.6147363138813056 1107 80-81 0.8567487154838211 1107 82-83 0.905806652656679 1107 84-85 0.971627113772275 1107 86-87 1.0392232554682685 1107 88-89 1.1123129152802314 1107 90-91 1.1603330552473068 1107 92-93 1.4443011947960294 1107 94-95 1.3752194060146188 1107 96-97 1.247137039736522 1107 98-99 1.3794276854953331 1107 100-101 1.4038719723904016 1108 1 0.10526062684151327 1108 2 0.16347953475988675 1108 3 0.2305932768403025 1108 4 0.17440763710619933 1108 5 0.08405969362269872 1108 6 0.15459672388458756 1108 7 0.13044927031883446 1108 8 0.024955670161546095 1108 9 0.06650273693912823 1108 10-11 0.12083474011820527 1108 12-13 0.18419872963264794 1108 14-15 0.34941530905956597 1108 16-17 0.25780299770809734 1108 18-19 0.26651364683907275 1108 20-21 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2316 86-87 -1.6290753416956214 2316 88-89 -1.516150997857288 2316 90-91 -1.578599070986268 2316 92-93 -1.8931959747668685 2316 94-95 -1.9162405599601868 2316 96-97 -1.7951464623777156 2316 98-99 -1.7503324640716507 2316 100-101 -1.7449781207430846 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 15.0 4 34.0 5 82.0 6 174.0 7 440.0 8 1029.0 9 1788.0 10 3000.0 11 4423.0 12 6390.0 13 8984.0 14 11632.0 15 15486.0 16 19213.0 17 24501.0 18 23140.0 19 19164.0 20 18856.0 21 19926.0 22 21679.0 23 24576.0 24 27965.0 25 32867.0 26 38207.0 27 45704.0 28 54324.0 29 64703.0 30 74932.0 31 88494.0 32 106179.0 33 129523.0 34 161826.0 35 207788.0 36 262015.0 37 285143.0 38 130260.0 39 5211.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.61052518700336 19.56563767772988 15.281560635798543 22.54227649946821 2 16.01135562989807 20.781431977115435 39.50316024456693 23.704052148419567 3 19.393662872226347 24.878845995215705 29.802647859440732 25.924843273117215 4 13.32777227572191 17.144331613632975 36.24940002959253 33.27849608105259 5 13.751352667320694 37.609361025231564 33.713214927876656 14.926071379571088 6 32.11184319062938 36.34207704363606 16.929751299183373 14.616328466551185 7 28.87734059226264 32.843768044213476 20.066454260496577 18.212437103027305 8 26.722552173554398 35.33412727872639 19.113513569248536 18.829806978470675 9 26.876340427286976 16.67331724820589 19.718974263796092 36.73136806071105 10-11 25.306416522312958 26.975686711210095 26.588664109543842 21.1292326569331 12-13 26.247035593499955 24.14364740575765 27.44576424977316 22.16355275096923 14-15 22.87699517446177 25.43628330446259 26.09494865132393 25.59177286975171 16-17 22.168373133712777 28.713292914295142 26.693732986884434 22.424600965107647 18-19 22.08054936896808 26.7608574610245 29.675461931865048 21.483131238142374 20-21 22.817320382743546 25.870813742472986 29.85598139899365 21.455884475789823 22-23 22.489353914047676 25.84843891775963 29.71941248040914 21.94279468778355 24-25 22.298161944781008 26.113840839369956 29.79134335283958 21.79665386300946 26-27 21.92292711270156 26.47564756501428 29.67646174028417 21.924963581999982 28-29 21.858106897612156 26.647070635674936 29.779857847576285 21.714964619136627 30-31 21.979308606153594 26.305173054684694 29.706616719434 22.008901619727713 32-33 21.839748307377423 26.344824564003883 29.569294737371226 22.24613239124747 34-35 22.447825238428305 26.409294121256387 29.40302497731924 21.739855662996067 36-37 22.467283502582898 26.12557514126333 29.34239550123182 22.064745854921945 38-39 22.040902711827005 26.421185433119987 29.479531114500162 22.058380740552842 40-41 22.28942201334206 26.291344004128568 29.441439472112446 21.977794510416928 42-43 22.153234780909596 26.284790846982524 29.213188293009363 22.348786079098517 44-45 22.128836231587403 26.543657363414606 29.10044903302447 22.227057371973512 46-47 22.327729316175866 26.865668563886825 28.536021405592678 22.270580714344636 48-49 22.33840117957601 26.79954941021199 28.468072053122164 22.393977357089828 50-51 22.478755373989454 26.91779052796176 28.316123334572378 22.28733076347641 52-53 22.689206467046112 26.91668213313536 28.114121917017236 22.279989482801287 54-55 22.19020321486415 26.9522307286405 28.30959146421784 22.547974592277512 56-57 22.357966262476285 27.174198836921555 28.128815062278314 22.33901983832385 58-59 22.45827597507323 26.82504980789764 28.093433350496767 22.623240866532367 60-61 22.40733007506393 26.90611337952652 28.373855481316507 22.312701064093048 62-63 22.312779117913276 27.005865746007757 28.26272293018672 22.418632205892248 64-65 22.019815815526773 26.813897368203943 28.30369297087699 22.862593845392297 66-67 21.991809389658776 27.057577629311723 28.15838101651012 22.792231964519384 68-69 22.237679951777384 26.823804399334 28.235316259818422 22.703199389070193 70-71 22.04053403695509 26.948770118346932 28.081328941883925 22.92936690281405 72-73 22.249789784804598 27.009701089716394 27.853603611731632 22.886905513747376 74-75 22.183308946286186 27.165125018532006 27.71364664780162 22.937919387380187 76-77 22.128639508451332 27.043919886067336 27.937307118367105 22.89013348711423 78-79 22.199983290717785 27.164470908830236 27.838050955059252 22.797494845392723 80-81 22.01262888864078 27.335646919191152 27.807534891372427 22.84418930079564 82-83 22.22162051624825 27.382065843997065 27.60997487533727 22.786338764417412 84-85 22.138424840692732 27.14194666828922 27.67341778156831 23.04621070944974 86-87 22.12649145357977 27.171717796693628 27.613385533241864 23.088405216484738 88-89 21.979810968414068 27.24781383531228 27.48625761939088 23.28611757688277 90-91 21.99419697637355 27.2643872106578 27.675183320213403 23.066232492755244 92-93 22.05367046778333 27.023459041949454 27.58460204819905 23.338268442068166 94-95 21.840041606169347 26.960596616378794 27.647397512963895 23.551964264487964 96-97 21.917492759412376 26.88591165129237 27.5462614946182 23.65033409467705 98-99 21.748014136142128 26.968596037196356 27.319420378968694 23.96396944769282 100-101 21.837014975704797 26.87946551839266 27.58340382427427 23.70011568162828 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1413.0 1 961.5 2 690.0 3 921.5 4 780.5 5 646.5 6 1151.5 7 2591.0 8 4078.5 9 4381.5 10 3509.0 11 2587.0 12 2406.5 13 2579.0 14 2770.5 15 2914.5 16 3060.5 17 3245.5 18 3454.0 19 3654.5 20 3955.5 21 4484.5 22 5297.5 23 6312.0 24 7392.0 25 8911.5 26 11002.0 27 13575.5 28 15893.5 29 18485.0 30 21793.0 31 25215.0 32 29549.5 33 32869.0 34 35547.0 35 38546.0 36 42011.0 37 45732.5 38 47959.5 39 49616.5 40 52453.0 41 56704.0 42 60184.0 43 63346.0 44 66497.5 45 69549.0 46 70753.5 47 72359.5 48 75735.5 49 75550.0 50 73216.5 51 70407.5 52 67500.0 53 66649.5 54 66782.0 55 63278.5 56 56950.0 57 52029.0 58 47451.0 59 41242.5 60 33889.0 61 28096.0 62 23574.0 63 18057.0 64 13495.0 65 10335.5 66 7675.0 67 6105.0 68 4680.0 69 3267.5 70 2443.0 71 1842.0 72 1506.5 73 1329.5 74 1072.0 75 892.5 76 621.5 77 348.5 78 220.0 79 137.5 80 86.0 81 56.0 82 29.5 83 16.0 84 12.0 85 8.0 86 3.5 87 3.0 88 1.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.639940608760208E-4 2 0.04670873546151942 3 0.0 4 5.1554895652891196E-5 5 0.04732739420935412 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006702136434875856 26-27 0.0025004124391652233 28-29 7.73323434793368E-5 30-31 0.0017013115565454096 32-33 6.444361956611399E-4 34-35 0.013430050317578157 36-37 0.02487523715252 38-39 0.005258599356594902 40-41 0.0012630949434958344 42-43 0.0018301987956776376 44-45 0.009228326321867524 46-47 0.0 48-49 0.0 50-51 6.702136434875856E-4 52-53 0.0 54-55 0.001727089004371855 56-57 0.0 58-59 0.024720572465561332 60-61 0.0 62-63 0.010285201682751795 64-65 0.016497566608925183 66-67 0.00732079518271055 68-69 0.0175802194176359 70-71 7.73323434793368E-5 72-73 5.1554895652891196E-5 74-75 0.024101913717726634 76-77 0.051090901592015174 78-79 0.032892023426544584 80-81 0.028587189639528172 82-83 0.03858883939618907 84-85 0.017837993895900354 86-87 0.010981192774065826 88-89 0.08748865792295638 90-91 0.026731213396024085 92-93 0.026447661469933183 94-95 0.028071640682999256 96-97 0.04039326074404025 98-99 0.05686504990513899 100-101 0.015750020621958263 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1939680.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.793410096765214 #Duplication Level Percentage of deduplicated Percentage of total 1 78.05285288880258 43.54834830447448 2 12.592910295542891 14.052028168620028 3 3.9380068884883332 6.591444998799478 4 1.711773430847879 3.8202270808016996 5 0.9543712753206204 2.6623813974268096 6 0.5689815931310053 1.9047254017841395 7 0.37749706891838886 1.4743294143543357 8 0.2762688819220651 1.2331186420842069 9 0.2022168739804969 1.0154132080631781 >10 1.1794283090147997 12.257082705619466 >50 0.0912533075816038 3.5016846471681218 >100 0.05028123299105436 4.896740138717803 >500 0.003141564835223382 1.221124870051751 >1k 7.391917259349132E-4 0.5566127083188566 >5k 1.847979314837283E-4 0.7392633358611097 >10k+ 9.239896574186416E-5 0.5254749778544137 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10193 0.52549905138992 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8245 0.4250701146580879 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6095 0.31422708900437185 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2075 0.10697640847974924 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.5777447826445598E-5 0.0 12-13 0.0 0.0 0.0 5.1554895652891196E-5 0.0 14-15 0.0 0.0 0.0 5.1554895652891196E-5 0.0 16-17 0.0 0.0 0.0 1.546646869586736E-4 0.0 18-19 0.0 0.0 0.0 3.093293739173472E-4 0.0 20-21 0.0 5.1554895652891196E-5 0.0 4.3821661304957514E-4 2.5777447826445598E-5 22-23 0.0 5.1554895652891196E-5 0.0 7.217685391404769E-4 5.1554895652891196E-5 24-25 0.0 5.1554895652891196E-5 0.0 0.0010053204652313782 5.1554895652891196E-5 26-27 0.0 5.1554895652891196E-5 0.0 0.0014435370782809537 5.1554895652891196E-5 28-29 0.0 5.1554895652891196E-5 0.0 0.0034284005609172647 5.1554895652891196E-5 30-31 0.0 5.1554895652891196E-5 0.0 0.009975872308834448 5.1554895652891196E-5 32-33 0.0 5.1554895652891196E-5 0.0 0.023405922626412605 5.1554895652891196E-5 34-35 0.0 5.1554895652891196E-5 0.0 0.04312567021364349 5.1554895652891196E-5 36-37 0.0 5.1554895652891196E-5 0.0 0.07581147405757652 5.1554895652891196E-5 38-39 0.0 5.1554895652891196E-5 0.0 0.1295574527757156 5.1554895652891196E-5 40-41 0.0 5.1554895652891196E-5 0.0 0.1875051554895653 5.1554895652891196E-5 42-43 0.0 5.1554895652891196E-5 0.0 0.24504041903819185 5.1554895652891196E-5 44-45 0.0 5.1554895652891196E-5 0.0 0.319382578569661 5.1554895652891196E-5 46-47 0.0 5.1554895652891196E-5 0.0 0.4110214055926751 5.1554895652891196E-5 48-49 0.0 5.1554895652891196E-5 0.0 0.5127134372680029 5.1554895652891196E-5 50-51 0.0 5.1554895652891196E-5 0.0 0.6229635816217107 5.1554895652891196E-5 52-53 0.0 5.1554895652891196E-5 0.0 0.7286511177101378 5.1554895652891196E-5 54-55 0.0 5.1554895652891196E-5 0.0 0.8473820423987461 5.1554895652891196E-5 56-57 0.0 5.1554895652891196E-5 0.0 0.9880753526354863 5.1554895652891196E-5 58-59 0.0 5.1554895652891196E-5 0.0 1.144776457972449 5.1554895652891196E-5 60-61 0.0 5.1554895652891196E-5 0.0 1.3184906788748658 5.1554895652891196E-5 62-63 0.0 5.1554895652891196E-5 0.0 1.4924111193598943 5.1554895652891196E-5 64-65 0.0 5.1554895652891196E-5 0.0 1.6723119277406582 5.1554895652891196E-5 66-67 0.0 5.1554895652891196E-5 0.0 1.8843572135609998 5.1554895652891196E-5 68-69 0.0 5.1554895652891196E-5 0.0 2.1188288789903487 5.1554895652891196E-5 70-71 0.0 5.1554895652891196E-5 0.0 2.377247793450466 5.1554895652891196E-5 72-73 0.0 5.1554895652891196E-5 0.0 2.6441474882454834 5.1554895652891196E-5 74-75 0.0 5.1554895652891196E-5 0.0 2.9097840880970054 5.1554895652891196E-5 76-77 0.0 5.1554895652891196E-5 0.0 3.203724325661965 5.1554895652891196E-5 78-79 0.0 5.1554895652891196E-5 0.0 3.5303761445186836 5.1554895652891196E-5 80-81 0.0 5.1554895652891196E-5 0.0 3.882960075888806 5.1554895652891196E-5 82-83 0.0 5.1554895652891196E-5 0.0 4.250649591685226 5.1554895652891196E-5 84-85 0.0 5.1554895652891196E-5 0.0 4.610399653551101 5.1554895652891196E-5 86-87 0.0 5.1554895652891196E-5 0.0 4.989225026808546 5.1554895652891196E-5 88-89 0.0 5.1554895652891196E-5 0.0 5.403700610409965 5.1554895652891196E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4985 0.0 49.292305 1 GTATCAA 8710 0.0 38.90676 1 ATCAACG 11220 0.0 29.977299 3 TATCAAC 11420 0.0 29.507597 2 TCAACGC 11490 0.0 29.272871 4 CAACGCA 11645 0.0 28.964827 5 AACGCAG 12040 0.0 28.000849 6 GTACATG 9845 0.0 26.117723 1 TACATGG 9775 0.0 25.769909 2 ACGCAGA 13625 0.0 24.604101 7 CGCAGAG 13715 0.0 24.408024 8 ACATGGG 9965 0.0 24.074993 3 TGGTATC 2400 0.0 22.576017 2 GAGTACT 8190 0.0 22.49506 12-13 GCAGAGT 14860 0.0 22.30365 9 GTGGTAT 2650 0.0 21.701674 1 GTACTTT 8560 0.0 21.43952 14-15 CATGGGA 5495 0.0 21.094742 4 AGTACTT 8520 0.0 20.815666 12-13 AGAGTAC 13830 0.0 20.445555 10-11 >>END_MODULE