FastQCFastQC Report
Thu 26 May 2016
SRR938302_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938302_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930404
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104350.5405604215490644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78500.40665062857308626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54760.28367119007213No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20450.10593637394037723No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50700.046.846521
GTATCAA87050.038.931041
TATCAAC111700.030.0372522
ATCAACG112300.029.7921333
TCAACGC114350.029.2580364
CAACGCA116150.028.844045
AACGCAG120000.028.073396
GTACATG92350.026.646391
TACATGG92850.026.256942
ACGCAGA135500.024.7542787
CGCAGAG137350.024.3516738
ACATGGG96500.024.3281063
GCAGAGT144950.022.3520439
GAGTACT81000.021.61018812-13
CATGGGG45750.021.6063274
TGGTATC27300.021.0635412
AGTACTT83800.020.91647112-13
TAAGGTG8300.020.6115465
GGACCGA4850.020.5734636
TAGCCCT11150.020.4585594
Produced by FastQC (version 0.11.5)