FastQCFastQC Report
Thu 26 May 2016
SRR938302_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938302_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930404
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99270.5142446866044621No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82680.428304127011755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61120.3166176613807265No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20940.10847470270471882No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48550.049.0734561
GTATCAA88550.041.1912421
ATCAACG112100.032.101073
TATCAAC113150.032.0256922
TCAACGC114050.031.5522144
CAACGCA116250.030.9959875
AACGCAG119300.030.4564196
ACGCAGA136150.026.5824587
CGCAGAG137800.026.2296988
GTACATG91250.026.1618841
TACATGG91550.025.4528182
GCAGAGT147600.023.9411189
ACATGGG94950.023.630683
GAGTACT84500.022.5114612-13
TGGTATC25400.022.0925772
AGTACTT87450.021.94216212-13
GTGGTAT27450.021.4821341
GTACTTT87700.021.47343314-15
CATGGGG41850.021.1274324
AGAGTAC137900.020.57934210-11
Produced by FastQC (version 0.11.5)