FastQCFastQC Report
Thu 26 May 2016
SRR938301_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938301_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934130
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104680.541225253731652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77880.4026616618324518No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54670.2826593869078087No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19500.10082052395650758No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48550.047.6053471
GTATCAA87200.040.9325331
ATCAACG110950.031.8664473
TATCAAC111800.031.7508682
TCAACGC112500.031.3851574
CAACGCA114300.030.807755
AACGCAG117500.030.006076
GTACATG93100.026.3097481
ACGCAGA135350.025.9739427
CGCAGAG135850.025.7734368
TACATGG93400.025.5407472
ACATGGG95600.024.506263
TGGTATC24750.023.808022
GCAGAGT143600.023.620939
CATGGGG44950.022.8355834
GTGGTAT26550.022.7951531
GAGTACT80000.022.14819512-13
AGTACTT81600.021.39373812-13
GTACTTT84950.020.71944214-15
AGAGTAC131700.020.50600410-11
Produced by FastQC (version 0.11.5)