FastQCFastQC Report
Thu 26 May 2016
SRR938301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938301_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934130
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99600.5149602146701617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82820.42820286123476703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61950.3202990491849048No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20950.10831743471224789No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53600.045.875061
GTATCAA91500.038.724521
ATCAACG117500.029.9424533
TATCAAC118550.029.7281272
TCAACGC119650.029.3646774
CAACGCA122550.028.7085955
AACGCAG127600.027.7307116
TGCACCG4200.024.9038735
ACGCAGA143050.024.6692757
GTACATG96800.024.6648921
TACATGG95500.024.5026282
CGCAGAG144600.024.3063018
ACATGGG97600.022.8463363
CCAATAC8950.022.8422533
GCAGAGT153050.022.5298739
GAGTACT85050.022.3936812-13
AGTACTT87350.021.45060312-13
GTACTTT89800.021.07693514-15
CCGTATA1150.003726081520.6503589
GCACCGT3950.020.441246
Produced by FastQC (version 0.11.5)