##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938301_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1934130 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.774257159549773 31.0 31.0 34.0 30.0 34.0 2 32.01270131790521 31.0 31.0 34.0 30.0 34.0 3 32.07125581010584 33.0 31.0 34.0 30.0 34.0 4 35.74584386778552 37.0 35.0 37.0 33.0 37.0 5 35.62074265949031 37.0 35.0 37.0 33.0 37.0 6 35.664288853386275 37.0 35.0 37.0 33.0 37.0 7 35.599057457358086 37.0 35.0 37.0 33.0 37.0 8 35.58646264728844 37.0 35.0 37.0 33.0 37.0 9 37.14279288362209 39.0 37.0 39.0 33.0 39.0 10-11 37.0762549570091 39.0 37.0 39.0 33.0 39.0 12-13 36.96017175681055 39.0 37.0 39.0 33.0 39.0 14-15 37.98040410934115 40.0 37.0 41.0 33.0 41.0 16-17 37.903819546772965 40.0 37.0 41.0 33.0 41.0 18-19 37.92083494904686 40.0 37.0 41.0 33.0 41.0 20-21 37.930938716632284 40.0 37.0 41.0 32.5 41.0 22-23 37.9524057328101 40.0 37.0 41.0 33.0 41.0 24-25 37.874894913992335 40.0 37.0 41.0 32.0 41.0 26-27 37.688783587452754 40.0 37.0 41.0 32.0 41.0 28-29 37.54549358109331 39.5 36.5 41.0 32.0 41.0 30-31 37.28358874532736 39.0 36.0 41.0 31.0 41.0 32-33 37.043861064147706 39.0 35.5 41.0 31.0 41.0 34-35 36.85179900006722 39.0 35.0 40.5 30.0 41.0 36-37 36.825735085025315 39.0 35.0 41.0 30.0 41.0 38-39 36.66806962303465 39.0 35.0 41.0 30.0 41.0 40-41 36.477394487443966 39.0 35.0 40.0 30.0 41.0 42-43 36.230327330634445 39.0 35.0 40.0 30.0 41.0 44-45 35.9048057783086 38.0 35.0 40.0 29.0 41.0 46-47 35.48249445487119 38.0 34.5 40.0 27.5 41.0 48-49 35.046730571368 38.0 34.0 40.0 26.0 41.0 50-51 34.65693981273234 38.0 33.5 40.0 24.5 41.0 52-53 34.13503099584827 38.0 33.0 40.0 23.5 41.0 54-55 33.799374912751475 37.0 33.0 40.0 23.0 41.0 56-57 33.432285575426675 37.0 32.0 40.0 22.0 41.0 58-59 32.97221101994178 36.5 31.5 39.0 20.5 41.0 60-61 32.55144483566255 36.0 31.0 39.0 19.0 40.0 62-63 32.06234198321726 35.5 31.0 39.0 17.5 40.0 64-65 31.547509733058273 35.0 30.0 38.5 13.5 40.0 66-67 31.047440709776488 35.0 29.5 38.0 10.0 40.0 68-69 30.476314415266813 34.0 29.0 37.5 7.5 39.5 70-71 29.899894526221093 34.0 28.0 37.0 6.5 39.0 72-73 29.30446790029626 34.0 27.5 36.0 2.0 39.0 74-75 28.66834597467595 33.0 26.0 36.0 2.0 38.5 76-77 27.411998934921645 31.5 25.5 34.5 2.0 36.5 78-79 28.02098928200276 33.0 26.0 35.0 2.0 37.0 80-81 27.699713049277968 33.0 26.0 35.0 2.0 37.0 82-83 27.194175675885283 33.0 25.5 35.0 2.0 36.0 84-85 26.590161209432665 32.0 24.5 35.0 2.0 36.0 86-87 25.928586754768293 32.0 23.0 34.5 2.0 35.0 88-89 25.166655550557614 31.0 20.0 34.0 2.0 35.0 90-91 24.607002373160025 31.0 18.0 34.0 2.0 35.0 92-93 23.811251570473544 31.0 9.0 34.0 2.0 35.0 94-95 23.01427153293729 30.0 2.0 34.0 2.0 35.0 96-97 22.025054675745686 30.0 2.0 34.0 2.0 35.0 98-99 20.85151308340184 29.0 2.0 34.0 2.0 35.0 100-101 17.791461794191704 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.41648209190661944 1101 2 0.36186720154327645 1101 3 0.26031353128335866 1101 4 0.24960721977036116 1101 5 0.25387059218871855 1101 6 0.27891538219527234 1101 7 0.29619873565615507 1101 8 0.3385420341957186 1101 9 0.3945962465515791 1101 10-11 0.4364187260255008 1101 12-13 0.39529990628495426 1101 14-15 0.5901474518883276 1101 16-17 0.6417324286552528 1101 18-19 0.7163440662178218 1101 20-21 0.708542008667564 1101 22-23 0.6439565868021546 1101 24-25 0.4999495122037132 1101 26-27 0.5354667536566922 1101 28-29 0.5292741017652816 1101 30-31 0.6683376977319 1101 32-33 0.5661074426432222 1101 34-35 0.47829622985096876 1101 36-37 0.689414178697163 1101 38-39 0.7971122970919993 1101 40-41 0.9779493457026049 1101 42-43 0.9668603760417724 1101 44-45 1.0199417200306513 1101 46-47 1.0636909664101069 1101 48-49 1.1009098171157135 1101 50-51 1.044442659105279 1101 52-53 1.2916376645402252 1101 54-55 1.3956744258808271 1101 56-57 1.3883320201211404 1101 58-59 1.4447578959446545 1101 60-61 1.6310896871728886 1101 62-63 1.6368627088442338 1101 64-65 1.6642779487867188 1101 66-67 1.7481602436370487 1101 68-69 1.7876367227613201 1101 70-71 1.826205163592494 1101 72-73 2.099761720376403 1101 74-75 2.0606216458737414 1101 76-77 1.9157758881973344 1101 78-79 2.23303115168828 1101 80-81 2.4124106574889765 1101 82-83 2.693403638067629 1101 84-85 2.798146327010752 1101 86-87 2.819802444037279 1101 88-89 3.0259938358362355 1101 90-91 3.169529460817518 1101 92-93 3.4654225885818093 1101 94-95 3.5222381478264033 1101 96-97 4.066232482529951 1101 98-99 4.5008626324891985 1101 100-101 4.887662594551912 1102 1 0.28999857678143215 1102 2 0.2512973163043455 1102 3 0.2298909866211858 1102 4 0.22681039755202193 1102 5 0.1009994463261421 1102 6 0.11436241004412295 1102 7 0.18892123423024998 1102 8 0.19555598599218627 1102 9 0.35374472961818526 1102 10-11 0.3000019944647079 1102 12-13 0.3449928290282642 1102 14-15 0.35740664169173897 1102 16-17 0.4231831258919101 1102 18-19 0.4824620905403165 1102 20-21 0.5654301453142949 1102 22-23 0.37591734307039104 1102 24-25 0.4152392755299559 1102 26-27 0.2864670944612371 1102 28-29 0.29423286088356804 1102 30-31 0.19782567863271794 1102 32-33 0.13321371185762843 1102 34-35 0.3233555351394486 1102 36-37 0.28540186735367 1102 38-39 0.42701356972327176 1102 40-41 0.4653139730160589 1102 42-43 0.3486497588323729 1102 44-45 0.23324607614843984 1102 46-47 0.54110339465133 1102 48-49 0.48788057679200136 1102 50-51 0.6067750159364707 1102 52-53 0.42936552239824977 1102 54-55 0.47371822544537423 1102 56-57 0.7061768463373141 1102 58-59 0.7788077134464615 1102 60-61 0.8977149452043136 1102 62-63 1.069659319163371 1102 64-65 0.8521685275717203 1102 66-67 0.9024414020899769 1102 68-69 0.8847209897754986 1102 70-71 1.0846087326813425 1102 72-73 1.1803452201389 1102 74-75 1.1448807138351107 1102 76-77 1.2018230322375807 1102 78-79 1.594945713931022 1102 80-81 1.8525288266092623 1102 82-83 1.837114999572961 1102 84-85 2.2278041804365465 1102 86-87 2.1238323253560694 1102 88-89 2.2732470924620607 1102 90-91 2.440870800408941 1102 92-93 2.7074463495453855 1102 94-95 2.765842765109962 1102 96-97 3.05367215747658 1102 98-99 3.290014099802864 1102 100-101 3.7415346992583736 1103 1 0.2622136650639355 1103 2 0.24713325446435874 1103 3 0.2144673150827856 1103 4 0.18993956879473473 1103 5 0.08866186078926575 1103 6 0.1944668847139468 1103 7 0.20558846410268217 1103 8 0.14488098388016368 1103 9 0.27671366882012194 1103 10-11 0.33471123948737613 1103 12-13 0.3695211441982167 1103 14-15 0.492981393177196 1103 16-17 0.42016969343436017 1103 18-19 0.4687353579785736 1103 20-21 0.44511024443851 1103 22-23 0.46186000872329913 1103 24-25 0.3529678666707312 1103 26-27 0.4275184588272083 1103 28-29 0.40801042276579835 1103 30-31 0.560229018442918 1103 32-33 0.5415932280638813 1103 34-35 0.5817337841327159 1103 36-37 0.6680610433836307 1103 38-39 0.5906717319828516 1103 40-41 0.7675987580122552 1103 42-43 0.7964504403113537 1103 44-45 0.8263617304527457 1103 46-47 0.834183989396621 1103 48-49 0.8234574402990802 1103 50-51 0.8736112075390778 1103 52-53 1.1207387588601847 1103 54-55 1.0901515841229639 1103 56-57 1.0482573284414514 1103 58-59 1.2788529107092828 1103 60-61 1.2133802320168456 1103 62-63 1.2633698595593046 1103 64-65 1.174115248306144 1103 66-67 1.2453410669825438 1103 68-69 1.3940654182624463 1103 70-71 1.1964364761974373 1103 72-73 1.3124043004238821 1103 74-75 1.5296141767057705 1103 76-77 1.3957605445577066 1103 78-79 1.5215946396160192 1103 80-81 1.9534543144862582 1103 82-83 2.142876453770249 1103 84-85 2.395927578820146 1103 86-87 2.458791211335061 1103 88-89 2.6651583312331475 1103 90-91 2.800269719053869 1103 92-93 3.098018848207772 1103 94-95 3.1688414134161214 1103 96-97 3.545123397937285 1103 98-99 3.8126147024330344 1103 100-101 4.055157238753175 1104 1 0.32779434337842517 1104 2 0.39690282226875695 1104 3 0.25552697863014373 1104 4 0.24497499909710996 1104 5 0.22086503058425677 1104 6 0.2304364849735876 1104 7 0.2354586431474175 1104 8 0.2863501243572557 1104 9 0.28840566860375105 1104 10-11 0.32610951935971855 1104 12-13 0.4434825765560859 1104 14-15 0.5820458709183072 1104 16-17 0.5807376601136554 1104 18-19 0.6675859016038217 1104 20-21 0.7158577967725819 1104 22-23 0.671982256789498 1104 24-25 0.5709962109126394 1104 26-27 0.5620251391972033 1104 28-29 0.6098124999010821 1104 30-31 0.6611683271463349 1104 32-33 0.6108091635861328 1104 34-35 0.6386878599697852 1104 36-37 0.816334424678594 1104 38-39 0.7986392226519712 1104 40-41 0.8368828495360319 1104 42-43 0.7925585162566122 1104 44-45 0.8472569541234307 1104 46-47 0.7964442263198634 1104 48-49 0.917867566333598 1104 50-51 1.0171558605384732 1104 52-53 1.1468903789175258 1104 54-55 1.0314992879148193 1104 56-57 1.029633971487911 1104 58-59 1.2937533813102462 1104 60-61 1.2189500919184013 1104 62-63 1.4072393354071977 1104 64-65 1.4177837721337028 1104 66-67 1.5205036136369507 1104 68-69 1.8400485415382661 1104 70-71 1.816969553494996 1104 72-73 2.0124448694724038 1104 74-75 2.0077425912673554 1104 76-77 1.9130264611477408 1104 78-79 2.197020073704838 1104 80-81 2.380881425429081 1104 82-83 2.4328929518499827 1104 84-85 2.540371782855413 1104 86-87 2.672302056794031 1104 88-89 2.5758133861365913 1104 90-91 2.7244891705603287 1104 92-93 3.167998293223185 1104 94-95 3.340175594191802 1104 96-97 3.8208590500171233 1104 98-99 4.368860172222618 1104 100-101 4.48304710730946 1105 1 0.2903472703673522 1105 2 0.29847735924440855 1105 3 0.2402863118352485 1105 4 0.21702242125991233 1105 5 0.18432411688846173 1105 6 0.2955777572548044 1105 7 0.23419819597954472 1105 8 0.28578331620255426 1105 9 0.3682365223588633 1105 10-11 0.37430865219302945 1105 12-13 0.46208334393519124 1105 14-15 0.6715891121175872 1105 16-17 0.5532969915368398 1105 18-19 0.47846589288288754 1105 20-21 0.4107539815221699 1105 22-23 0.41146171569342016 1105 24-25 0.28384577536393607 1105 26-27 0.5452517581642837 1105 28-29 0.5190575523183867 1105 30-31 0.6209245550917828 1105 32-33 0.5913775754698705 1105 34-35 0.5907885091655913 1105 36-37 0.6608657348675635 1105 38-39 0.6638651885973701 1105 40-41 0.5874271216291049 1105 42-43 0.7188946819710864 1105 44-45 0.72809097707772 1105 46-47 0.8035104608531753 1105 48-49 0.9174151417940237 1105 50-51 0.8613516192516855 1105 52-53 0.898712000224684 1105 54-55 1.0384490424551842 1105 56-57 1.232688472594539 1105 58-59 1.3037933277897267 1105 60-61 1.1798256545993127 1105 62-63 1.3308838830167247 1105 64-65 1.4906065658401673 1105 66-67 1.5997791893154023 1105 68-69 1.4107923835594214 1105 70-71 1.451463852133454 1105 72-73 1.815841053959943 1105 74-75 2.0121786577707503 1105 76-77 1.8522774934827986 1105 78-79 2.119426841231739 1105 80-81 2.349383217570079 1105 82-83 2.25036041737636 1105 84-85 2.327826717283333 1105 86-87 2.4263822631614644 1105 88-89 2.7833568872867147 1105 90-91 2.9957544341562574 1105 92-93 3.373327364271006 1105 94-95 3.4422367004608603 1105 96-97 3.6861946827275354 1105 98-99 4.172210236397984 1105 100-101 4.237557464202062 1106 1 0.1328793076650605 1106 2 0.22956756604977357 1106 3 0.17089790769334456 1106 4 0.11496287976032704 1106 5 0.10468147972908781 1106 6 0.07673087803056688 1106 7 0.13160808304250793 1106 8 0.07021151450780394 1106 9 0.2597263079448169 1106 10-11 0.2901180360486748 1106 12-13 0.16119320068351328 1106 14-15 0.018849423467976578 1106 16-17 0.08718319493003435 1106 18-19 0.20745446758053987 1106 20-21 0.3225770296811845 1106 22-23 0.1303142087394633 1106 24-25 0.15979245502333583 1106 26-27 0.14839659502792557 1106 28-29 0.21214077552943422 1106 30-31 0.2675541862010249 1106 32-33 0.3559623497929252 1106 34-35 0.24008692504951767 1106 36-37 0.3687625282786655 1106 38-39 0.4402660133852905 1106 40-41 0.40104810719165584 1106 42-43 0.44989992423622027 1106 44-45 -0.019982894550629737 1106 46-47 -0.1500142750817801 1106 48-49 0.4159658618906974 1106 50-51 0.11076484431919909 1106 52-53 0.4216217458976743 1106 54-55 0.5316153241181141 1106 56-57 0.3774690674664285 1106 58-59 0.9159864704149996 1106 60-61 0.6522642700689687 1106 62-63 0.7279551130155255 1106 64-65 0.4192563268088527 1106 66-67 0.6149810270978868 1106 68-69 0.46666061593404606 1106 70-71 0.7670362282657806 1106 72-73 1.0528346774416981 1106 74-75 1.2396556783092443 1106 76-77 1.5035742993748578 1106 78-79 1.7687175544299372 1106 80-81 1.6340346632075224 1106 82-83 1.9022179526788143 1106 84-85 1.9340872252480388 1106 86-87 1.892177769127393 1106 88-89 2.2754361763836535 1106 90-91 2.299319628774402 1106 92-93 1.9616850028416692 1106 94-95 2.492385623403454 1106 96-97 2.8827625226307383 1106 98-99 2.8393188283391986 1106 100-101 3.1944880927699373 1107 1 0.08719413381393437 1107 2 0.06883452913540822 1107 3 0.05326562922752487 1107 4 0.10594080234736936 1107 5 0.08118447592084266 1107 6 0.05506824162183932 1107 7 0.15449779798426277 1107 8 0.14418299868279405 1107 9 0.09462074118854247 1107 10-11 0.1329092246847452 1107 12-13 0.1388133933758482 1107 14-15 0.2831402130785108 1107 16-17 0.28131586942120634 1107 18-19 0.25460861044567906 1107 20-21 0.20038008407667007 1107 22-23 0.13790544457855702 1107 24-25 0.2067903378878384 1107 26-27 0.3227821307022083 1107 28-29 0.333787560923696 1107 30-31 0.34791507929818266 1107 32-33 0.35240274697834906 1107 34-35 0.46899632525113333 1107 36-37 0.3260297933836327 1107 38-39 0.45899821224601567 1107 40-41 0.4421844899201517 1107 42-43 0.6763024139955647 1107 44-45 0.6126112164724802 1107 46-47 0.5915256834755667 1107 48-49 0.6738084888188212 1107 50-51 0.8678792996443363 1107 52-53 0.8051379159325549 1107 54-55 0.6952559221163881 1107 56-57 0.8644441088690797 1107 58-59 0.8294373062684954 1107 60-61 0.963180293694478 1107 62-63 0.9315143908093049 1107 64-65 0.9355949974837756 1107 66-67 1.1557203844167567 1107 68-69 1.4343136707295514 1107 70-71 1.4868204194540162 1107 72-73 1.570368424601515 1107 74-75 1.531352971936034 1107 76-77 1.523634208209021 1107 78-79 1.6259799192212832 1107 80-81 1.974083426328363 1107 82-83 2.0607079710399887 1107 84-85 2.3982106791490914 1107 86-87 2.6654703848547996 1107 88-89 2.8197836396213027 1107 90-91 2.9499130167512355 1107 92-93 3.0452063482077705 1107 94-95 3.35490432460033 1107 96-97 3.5562485007333358 1107 98-99 3.8380068693738245 1107 100-101 4.037024015068965 1108 1 0.17376572793230238 1108 2 0.17093327514474055 1108 3 0.17334540421524736 1108 4 0.18805455173342267 1108 5 0.08680786430493015 1108 6 0.13979415180111232 1108 7 0.20558122596805362 1108 8 0.17796649727070957 1108 9 0.18000184998029312 1108 10-11 0.2082858439750197 1108 12-13 0.29262630395418654 1108 14-15 0.3765280791455581 1108 16-17 0.364693010568061 1108 18-19 0.4941153600466137 1108 20-21 0.47845529665648456 1108 22-23 0.3272942968010497 1108 24-25 0.35974276068375843 1108 26-27 0.32953221128300214 1108 28-29 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0.6583313695878452 2316 74-75 0.8573331261668997 2316 76-77 0.824872190471698 2316 78-79 0.9073056367908627 2316 80-81 0.952661178564945 2316 82-83 1.309352335323041 2316 84-85 1.4903599320690724 2316 86-87 1.6213605322516678 2316 88-89 2.1695352277487885 2316 90-91 1.9880504033690265 2316 92-93 1.9799572179890816 2316 94-95 2.7689648831241875 2316 96-97 2.7363471083066457 2316 98-99 2.922053856979442 2316 100-101 3.2932445046315877 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 13.0 4 28.0 5 88.0 6 153.0 7 413.0 8 907.0 9 1695.0 10 2805.0 11 4390.0 12 6156.0 13 8990.0 14 11719.0 15 15250.0 16 19539.0 17 24233.0 18 23436.0 19 19698.0 20 19064.0 21 20819.0 22 22503.0 23 25610.0 24 29052.0 25 33437.0 26 39448.0 27 46531.0 28 54676.0 29 65101.0 30 75962.0 31 89463.0 32 106095.0 33 127923.0 34 157715.0 35 199476.0 36 245553.0 37 274707.0 38 152195.0 39 9285.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.65289440363078 19.598847853478233 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29.216921589781254 22.356890292187572 44-45 22.133687197675165 26.571882313737138 29.111520781645172 22.182909706942517 46-47 22.33005140815323 26.88559036134611 28.47338517751401 22.31097305298664 48-49 22.298346026378784 26.8562868059541 28.45868685145259 22.386680316214523 50-51 22.46428626824464 26.958348197897763 28.28920496554006 22.28816056831754 52-53 22.628572019460947 26.986035064861202 28.09247568674288 22.29291722893497 54-55 22.209701519546257 26.94979137906966 28.28897230279247 22.551534798591614 56-57 22.292736269020182 27.170536623701615 28.133579438817453 22.403147668460754 58-59 22.43566875029083 26.815700082207506 28.112407128786586 22.63622403871508 60-61 22.374038973595365 26.93852016151965 28.357090785004107 22.330350079880876 62-63 22.262800328830014 26.9861384705268 28.312367834633662 22.438693366009524 64-65 22.03931483405976 26.825497769022768 28.302544296401997 22.832643100515476 66-67 22.005417434024462 27.05482993352036 28.167890819936243 22.77186181251894 68-69 22.199162723026976 26.856431868227887 28.193831118639057 22.75057429010608 70-71 22.092801414589506 27.00663864373129 27.99475733275426 22.905802608924944 72-73 22.288166330124223 27.028800294913037 27.804782461567335 22.878250913395405 74-75 22.10479647179869 27.29064230429185 27.656439846339183 22.948121377570278 76-77 22.12529664500318 27.008758459876013 27.945872304343556 22.92007259077725 78-79 22.20758170340153 27.141893254331407 27.821604545713058 22.828920496554005 80-81 22.04420645049879 27.374976701404947 27.823550594724917 22.75726625337135 82-83 22.16460451934315 27.448149884042184 27.670422833052022 22.716822763562643 84-85 22.14211646550318 27.12748259408218 27.708287391086174 23.022113549328466 86-87 22.13881557738657 27.174882768380204 27.610693043813434 23.07560861041979 88-89 22.005719370321213 27.327391296030413 27.46910883400228 23.197780499646097 90-91 21.974284276192364 27.29805489195668 27.69984207194167 23.02781875990928 92-93 22.04446719377909 27.09194996697469 27.623741515855382 23.239841323390838 94-95 21.838423477222317 27.007026414977275 27.678930578606405 23.475619529194006 96-97 21.93883037851644 26.931333467760698 27.55949703484254 23.570339118880323 98-99 21.750140890219374 27.02605305744702 27.345550712723547 23.878255339610057 100-101 21.81684035497354 26.888899114634285 27.60524704826391 23.689013482128267 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1431.0 1 944.0 2 716.5 3 960.0 4 755.0 5 695.5 6 1233.0 7 2709.0 8 4200.0 9 4552.0 10 3715.0 11 2671.0 12 2493.5 13 2636.5 14 2837.5 15 2993.5 16 3092.5 17 3279.0 18 3460.5 19 3660.5 20 3953.0 21 4479.5 22 5235.0 23 6174.0 24 7354.5 25 8896.0 26 11021.0 27 13608.0 28 15841.5 29 18422.5 30 21796.5 31 25263.0 32 29391.5 33 32466.5 34 35018.5 35 38196.5 36 41925.0 37 45442.0 38 47569.5 39 49447.0 40 52274.5 41 56720.0 42 60376.0 43 63290.5 44 66165.0 45 68894.5 46 70496.5 47 72041.5 48 75301.5 49 75553.5 50 72843.5 51 69853.0 52 67031.0 53 66294.5 54 66390.0 55 62897.5 56 56818.5 57 51830.0 58 47253.0 59 41177.0 60 34071.0 61 28333.5 62 23725.0 63 18242.5 64 13479.5 65 10293.0 66 7647.0 67 6086.5 68 4784.0 69 3402.0 70 2483.0 71 1882.0 72 1536.5 73 1308.5 74 1072.0 75 871.0 76 632.5 77 373.0 78 222.5 79 137.5 80 85.0 81 57.5 82 33.0 83 16.0 84 8.0 85 7.5 86 9.5 87 6.0 88 1.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.1702832798209016E-5 2 0.042086105897742136 3 2.585141639910451E-4 4 0.0 5 0.10449142508518043 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0013442736527534344 26-27 0.011865800127188969 28-29 0.0 30-31 0.0 32-33 3.6191982958746306E-4 34-35 0.01525233567547166 36-37 0.003257278466287168 38-39 0.012176017123978223 40-41 0.007264248008148367 42-43 0.0059716771881931415 44-45 0.0028695072203006006 46-47 5.1702832798209016E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.0681133119283606E-4 68-69 1.5510849839462705E-4 70-71 0.0 72-73 2.5851416399104508E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.5851416399104508E-5 82-83 0.0011116109051614938 84-85 0.004627403535439707 86-87 0.001628639233143584 88-89 5.1702832798209016E-5 90-91 0.0011116109051614938 92-93 3.8777124598656757E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.238396591749261E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1934130.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.8083165665557 #Duplication Level Percentage of deduplicated Percentage of total 1 78.21696138396874 43.6515694179059 2 12.541530072940981 13.998433610793374 3 3.8351699695686836 6.421031392447107 4 1.709581443296055 3.8163544953510176 5 0.9269272792640887 2.5865125517673224 6 0.557730003480116 1.8675583555730717 7 0.3929792504935959 1.5352057290944072 8 0.2848398681548203 1.271714682620816 9 0.19843103224307873 0.9966691677645144 >10 1.1898465649288628 12.376959659039882 >50 0.09205994746485943 3.5576867959775327 >100 0.04976623605235988 4.868344785347226 >500 0.0029702742354970747 1.1143619674300453 >1k 9.282106985928353E-4 0.6717149326731775 >5k 2.784632095778506E-4 1.2658824562145783 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9960 0.5149602146701617 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8282 0.42820286123476703 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6195 0.3202990491849048 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2095 0.10831743471224789 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.1702832798209016E-5 0.0 10-11 0.0 0.0 0.0 1.5510849839462705E-4 0.0 12-13 0.0 0.0 0.0 2.843655803901496E-4 0.0 14-15 0.0 0.0 0.0 3.102169967892541E-4 0.0 16-17 0.0 0.0 0.0 4.3947407878477664E-4 0.0 18-19 0.0 0.0 0.0 6.204339935785082E-4 0.0 20-21 0.0 0.0 0.0 6.979882427758217E-4 0.0 22-23 0.0 0.0 0.0 7.238396591749262E-4 0.0 24-25 0.0 0.0 0.0 8.530967411704488E-4 0.0 26-27 0.0 0.0 0.0 0.0014218279019507479 0.0 28-29 0.0 0.0 0.0 0.0036450497122737357 0.0 30-31 0.0 0.0 0.0 0.010107903812049863 0.0 32-33 0.0 0.0 0.0 0.02404181725116719 0.0 34-35 0.0 0.0 0.0 0.046506698101989014 0.0 36-37 0.0 0.0 0.0 0.08445657737587442 0.0 38-39 0.0 0.0 0.0 0.14001127121755 0.0 40-41 0.0 0.0 0.0 0.20003826009627068 0.0 42-43 0.0 0.0 0.0 0.2615129282933412 0.0 44-45 0.0 0.0 0.0 0.34015293697941706 0.0 46-47 0.0 0.0 0.0 0.43414868700656106 0.0 48-49 0.0 0.0 0.0 0.5372441356061899 0.0 50-51 0.0 0.0 0.0 0.649594391276698 0.0 52-53 0.0 0.0 0.0 0.7618153898652107 2.5851416399104508E-5 54-55 0.0 0.0 0.0 0.8816625562914592 5.1702832798209016E-5 56-57 0.0 0.0 0.0 1.0256807970508706 5.1702832798209016E-5 58-59 0.0 0.0 0.0 1.1814872836882733 5.1702832798209016E-5 60-61 0.0 0.0 0.0 1.3579749034449597 5.1702832798209016E-5 62-63 0.0 0.0 0.0 1.5345917802836417 5.1702832798209016E-5 64-65 0.0 0.0 0.0 1.7202825042784093 5.1702832798209016E-5 66-67 0.0 0.0 0.0 1.9347975575581786 5.1702832798209016E-5 68-69 0.0 0.0 0.0 2.173561239420308 5.1702832798209016E-5 70-71 0.0 0.0 0.0 2.4327733916541288 5.1702832798209016E-5 72-73 0.0 0.0 0.0 2.6967422045053846 5.1702832798209016E-5 74-75 0.0 0.0 0.0 2.966372477548045 5.1702832798209016E-5 76-77 0.0 0.0 0.0 3.263948131718137 5.1702832798209016E-5 78-79 0.0 0.0 0.0 3.5903481151732306 5.1702832798209016E-5 80-81 0.0 0.0 0.0 3.944254005676971 5.1702832798209016E-5 82-83 0.0 0.0 0.0 4.310284210471892 5.1702832798209016E-5 84-85 0.0 0.0 0.0 4.680528196139866 5.1702832798209016E-5 86-87 0.0 0.0 0.0 5.062948198931819 5.1702832798209016E-5 88-89 0.0 0.0 0.0 5.472072714864048 5.1702832798209016E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5360 0.0 45.87506 1 GTATCAA 9150 0.0 38.72452 1 ATCAACG 11750 0.0 29.942453 3 TATCAAC 11855 0.0 29.728127 2 TCAACGC 11965 0.0 29.364677 4 CAACGCA 12255 0.0 28.708595 5 AACGCAG 12760 0.0 27.730711 6 TGCACCG 420 0.0 24.903873 5 ACGCAGA 14305 0.0 24.669275 7 GTACATG 9680 0.0 24.664892 1 TACATGG 9550 0.0 24.502628 2 CGCAGAG 14460 0.0 24.306301 8 ACATGGG 9760 0.0 22.846336 3 CCAATAC 895 0.0 22.842253 3 GCAGAGT 15305 0.0 22.529873 9 GAGTACT 8505 0.0 22.39368 12-13 AGTACTT 8735 0.0 21.450603 12-13 GTACTTT 8980 0.0 21.076935 14-15 CCGTATA 115 0.0037260815 20.650358 9 GCACCGT 395 0.0 20.44124 6 >>END_MODULE