FastQCFastQC Report
Thu 26 May 2016
SRR938300_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938300_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916998
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98590.5142937029668263No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74860.3905064063707943No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53110.27704775904826195No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19400.10119989692216685No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44800.049.4123951
GTATCAA79600.040.8758931
ATCAACG104100.031.0885073
TATCAAC104850.030.7754782
TCAACGC106000.030.5312584
CAACGCA108350.029.7813475
AACGCAG112900.028.7014086
GTACATG92300.025.8442691
TAGCGCG1501.0562708E-625.345584
TACATGG92750.025.2089442
ACGCAGA130350.024.7088267
TGGTATC22600.024.6025962
CGCAGAG131200.024.476338
GTGGTAT25400.023.6664331
ACATGGG96350.023.5271933
GCAGAGT137000.023.0921549
GAGTACT78400.021.7509312-13
AGTACTT81600.021.1890112-13
GTGTAGC10800.020.7620411
AGAGTAC127950.020.25108310-11
Produced by FastQC (version 0.11.5)