##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938300_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1916998 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.076444524198774 31.0 31.0 34.0 28.0 34.0 2 31.249842201191655 31.0 31.0 34.0 28.0 34.0 3 31.2970936850221 31.0 31.0 34.0 27.0 34.0 4 34.96883825648227 37.0 35.0 37.0 32.0 37.0 5 34.96430825697262 37.0 35.0 37.0 32.0 37.0 6 34.944175737272545 37.0 35.0 37.0 32.0 37.0 7 34.85846255447319 37.0 35.0 37.0 32.0 37.0 8 34.812599700156184 37.0 35.0 37.0 32.0 37.0 9 36.277168781605404 39.0 35.0 39.0 32.0 39.0 10-11 36.106549146112826 38.5 35.0 39.0 31.0 39.0 12-13 35.91091096599997 38.0 35.0 39.0 30.0 39.0 14-15 37.044902498594155 39.0 36.0 41.0 31.0 41.0 16-17 36.97308943462643 39.0 36.0 41.0 31.0 41.0 18-19 36.984758460885196 39.0 36.0 41.0 31.0 41.0 20-21 36.924318909044246 39.0 36.0 41.0 30.5 41.0 22-23 36.76045619244256 39.0 36.0 41.0 30.0 41.0 24-25 36.60499932707285 39.0 36.0 41.0 30.0 41.0 26-27 36.38355908561198 39.0 35.5 41.0 29.5 41.0 28-29 36.13015819526155 39.0 35.0 40.0 29.0 41.0 30-31 35.83210545863898 38.5 35.0 40.0 27.5 41.0 32-33 35.50224100390298 38.0 34.5 40.0 27.0 41.0 34-35 35.17016188853614 38.0 34.0 40.0 26.0 41.0 36-37 34.90912066679256 38.0 34.0 40.0 25.0 41.0 38-39 34.641535098106516 38.0 33.5 40.0 24.5 41.0 40-41 34.33672805083782 38.0 33.0 40.0 24.0 41.0 42-43 33.893347828218914 38.0 33.0 40.0 22.5 41.0 44-45 33.36509558173769 37.5 32.5 40.0 19.5 41.0 46-47 32.81072202474911 37.0 31.0 40.0 16.0 41.0 48-49 32.269494543030305 37.0 30.5 40.0 11.0 41.0 50-51 31.52858531933784 36.0 30.0 38.5 9.0 40.5 52-53 31.517922814734288 36.0 30.0 39.0 8.0 40.0 54-55 31.476144993369843 36.0 30.0 39.0 4.5 40.0 56-57 31.03126372588808 36.0 29.0 39.0 2.0 40.0 58-59 30.557580915577375 35.0 28.5 39.0 2.0 40.0 60-61 29.99141887471974 35.0 28.0 38.5 2.0 40.0 62-63 29.24656363752075 34.0 26.5 38.0 2.0 40.0 64-65 28.569528241552675 34.0 26.0 38.0 2.0 40.0 66-67 27.854248152580233 33.5 24.5 37.0 2.0 39.5 68-69 26.98994625972484 33.0 22.0 36.5 2.0 39.0 70-71 26.138655856709292 32.0 19.5 36.0 2.0 39.0 72-73 25.22294911105802 31.5 15.5 35.0 2.0 38.0 74-75 24.27462913367672 31.0 7.5 35.0 2.0 37.5 76-77 23.31815187078964 30.0 2.0 34.5 2.0 36.5 78-79 22.32758902200211 29.0 2.0 34.0 2.0 36.0 80-81 21.35034256686757 28.5 2.0 34.0 2.0 35.5 82-83 20.3382945626443 26.5 2.0 33.5 2.0 35.0 84-85 19.3620543683405 25.5 2.0 33.0 2.0 35.0 86-87 18.369455262864122 24.5 2.0 32.0 2.0 34.5 88-89 17.295253829164142 20.5 2.0 32.0 2.0 34.0 90-91 16.157575542593158 13.0 2.0 31.0 2.0 34.0 92-93 15.010448628532737 2.0 2.0 31.0 2.0 34.0 94-95 13.771775713902674 2.0 2.0 30.0 2.0 34.0 96-97 12.390570308367561 2.0 2.0 29.0 2.0 34.0 98-99 10.75223004927496 2.0 2.0 27.0 2.0 33.5 100-101 7.580136233840619 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6317318073278742 1101 2 0.5830065347064348 1101 3 0.4566441903570464 1101 4 0.3220159529334339 1101 5 0.3597533565096711 1101 6 0.5088007583699792 1101 7 0.4306730172368134 1101 8 0.507918959726041 1101 9 0.48498502246209796 1101 10-11 0.6398740594222119 1101 12-13 0.5287628621812814 1101 14-15 0.6681134329915537 1101 16-17 0.6605065289046266 1101 18-19 0.7903858457263979 1101 20-21 0.8285351394640443 1101 22-23 0.7871305135619551 1101 24-25 0.9867034044149605 1101 26-27 0.8708507894429118 1101 28-29 1.1023004077183103 1101 30-31 1.2096772842377348 1101 32-33 1.1971086995304248 1101 34-35 1.2212978206974654 1101 36-37 1.2860366550090063 1101 38-39 1.5783231263758566 1101 40-41 1.4439014213027477 1101 42-43 1.693401824431497 1101 44-45 1.888057119171357 1101 46-47 1.9644173594235568 1101 48-49 1.7108445987237673 1101 50-51 1.952315391811041 1101 52-53 1.9249322723968483 1101 54-55 2.0763128053010327 1101 56-57 2.4970475582806415 1101 58-59 2.362669036268926 1101 60-61 2.4174204144268145 1101 62-63 2.320993117949129 1101 64-65 2.319681683700793 1101 66-67 2.587035498495144 1101 68-69 2.573045565951883 1101 70-71 2.7443364210588115 1101 72-73 3.0243279109298555 1101 74-75 3.104590375106259 1101 76-77 3.1418612144725024 1101 78-79 3.3320425598996835 1101 80-81 3.626906150433193 1101 82-83 3.5873338121729574 1101 84-85 3.5168623187110946 1101 86-87 3.5363178252789957 1101 88-89 3.96631101735521 1101 90-91 3.8058897123136397 1101 92-93 3.9874121108871385 1101 94-95 3.905138538423495 1101 96-97 3.671648205255627 1101 98-99 3.6754073192551324 1101 100-101 2.799379056239438 1102 1 0.6736621646896772 1102 2 0.5906142666992302 1102 3 0.5791554374922114 1102 4 0.3770479259214454 1102 5 0.5013529019084828 1102 6 0.579242721266219 1102 7 0.5775624887865192 1102 8 0.59934378547797 1102 9 0.5813425540342436 1102 10-11 0.772958674661723 1102 12-13 0.8227451732907198 1102 14-15 0.937247710109645 1102 16-17 1.0053992213320342 1102 18-19 0.9026974503176817 1102 20-21 0.9022144852910472 1102 22-23 0.8162374863141793 1102 24-25 0.8314923496836144 1102 26-27 0.8290707475667887 1102 28-29 0.8031378123416673 1102 30-31 0.6409500968232038 1102 32-33 0.905152405378935 1102 34-35 0.9465617864797693 1102 36-37 1.1777246212817403 1102 38-39 1.2726055968786554 1102 40-41 1.3142369254667798 1102 42-43 1.6263746756863569 1102 44-45 1.4372863249396985 1102 46-47 1.3754636023616769 1102 48-49 1.7499720143820383 1102 50-51 1.8725638898351278 1102 52-53 1.8292826972886225 1102 54-55 1.7785643544347387 1102 56-57 1.8518245254682455 1102 58-59 2.013889951696129 1102 60-61 2.044555483795932 1102 62-63 2.1358286079658235 1102 64-65 2.196835616189688 1102 66-67 2.3903680222862533 1102 68-69 2.3253728033536802 1102 70-71 2.2526468042630903 1102 72-73 2.402468620371792 1102 74-75 2.6203051222932956 1102 76-77 2.77946413636975 1102 78-79 3.233134970323441 1102 80-81 3.294481390702181 1102 82-83 3.269798538138982 1102 84-85 3.385669320701151 1102 86-87 3.397686863354206 1102 88-89 3.5648784813030723 1102 90-91 3.6477041793833465 1102 92-93 4.036204517538273 1102 94-95 3.6967264122669494 1102 96-97 3.472561533936057 1102 98-99 3.28713770396846 1102 100-101 2.492171340280297 1103 1 0.5025037323642643 1103 2 0.47185751723918656 1103 3 0.5199356750732065 1103 4 0.3898516516233954 1103 5 0.31197542710646786 1103 6 0.26245568922878704 1103 7 0.3680547494056654 1103 8 0.4436494910215316 1103 9 0.5816507793762185 1103 10-11 0.5710615139345805 1103 12-13 0.541889454248242 1103 14-15 0.7284891607935862 1103 16-17 0.6676219310152547 1103 18-19 0.6610133475459037 1103 20-21 0.7785863130448334 1103 22-23 0.8053528570845145 1103 24-25 0.8086909862926817 1103 26-27 0.9059251617136539 1103 28-29 0.8011461591739177 1103 30-31 0.8385084796525817 1103 32-33 0.8872162777691486 1103 34-35 0.8138139688051851 1103 36-37 0.987071498531563 1103 38-39 0.9946611449348097 1103 40-41 1.1348857735589277 1103 42-43 1.1945003506323673 1103 44-45 1.1719419899864931 1103 46-47 1.3551640388267572 1103 48-49 1.2017516220134326 1103 50-51 1.4657638088416007 1103 52-53 1.5078857840416795 1103 54-55 1.4808479217493549 1103 56-57 1.7842905531860325 1103 58-59 1.6665070548278749 1103 60-61 1.7659090880076036 1103 62-63 1.905228243494978 1103 64-65 2.3074170821724103 1103 66-67 2.18526261821858 1103 68-69 2.4135477493579955 1103 70-71 2.6615341557749694 1103 72-73 2.8943242719196647 1103 74-75 2.8095155479592435 1103 76-77 2.8922251154914242 1103 78-79 2.9205955984731915 1103 80-81 3.0165088160081552 1103 82-83 3.2533591032937714 1103 84-85 3.408494869657236 1103 86-87 3.4912782510431875 1103 88-89 3.5621477622605973 1103 90-91 3.428742696301992 1103 92-93 3.2772035501156687 1103 94-95 3.3736235729940933 1103 96-97 3.2273682743968184 1103 98-99 2.963568027042614 1103 100-101 2.3808508084228457 1104 1 0.5771833646284463 1104 2 0.5641767748698108 1104 3 0.5749570149593914 1104 4 0.46805425120953004 1104 5 0.4119385134144551 1104 6 0.5521610802444741 1104 7 0.47769757575503746 1104 8 0.39061933496013523 1104 9 0.6538900663222407 1104 10-11 0.6453640607098805 1104 12-13 0.7806117824513805 1104 14-15 0.8209614620524306 1104 16-17 0.9215222532690959 1104 18-19 0.8461052006671324 1104 20-21 0.905614006397137 1104 22-23 0.9898986839512176 1104 24-25 0.916151459003494 1104 26-27 0.8881027192666835 1104 28-29 1.0469470246248633 1104 30-31 0.990857887955741 1104 32-33 0.8734018000574721 1104 34-35 1.0392252735559993 1104 36-37 1.0140893572140968 1104 38-39 0.9874391292855549 1104 40-41 1.1438625367939679 1104 42-43 1.28818810929765 1104 44-45 1.168499990288268 1104 46-47 1.2722296158659034 1104 48-49 1.1191959011994967 1104 50-51 1.2298715752668556 1104 52-53 1.427688497871646 1104 54-55 1.6667307248188514 1104 56-57 1.891363162687668 1104 58-59 1.910720631522139 1104 60-61 1.971154739197459 1104 62-63 2.153277341399793 1104 64-65 2.1277881860265246 1104 66-67 2.245816027211511 1104 68-69 2.625808483967411 1104 70-71 2.833355401894991 1104 72-73 2.8941410506746266 1104 74-75 2.706581448329999 1104 76-77 2.692686401920117 1104 78-79 2.9613528949898296 1104 80-81 3.061600507463581 1104 82-83 2.9469792317641996 1104 84-85 3.1582251572068145 1104 86-87 3.212903477375317 1104 88-89 3.355323983376305 1104 90-91 3.378693118788629 1104 92-93 3.325648459798373 1104 94-95 3.052787141066174 1104 96-97 2.9690470659988204 1104 98-99 2.721291745571671 1104 100-101 1.891675546524242 1105 1 0.46666977775856466 1105 2 0.4034864933823279 1105 3 0.3164938261666457 1105 4 0.36241005122577974 1105 5 0.28181161829268575 1105 6 0.30160723826788427 1105 7 0.3707096533155436 1105 8 0.37962323190953384 1105 9 0.49795089534705994 1105 10-11 0.5403483968040277 1105 12-13 0.575033817236978 1105 14-15 0.7401463012839855 1105 16-17 0.6605917164691988 1105 18-19 0.7441680650740707 1105 20-21 0.7540258632436334 1105 22-23 0.5496330095033386 1105 24-25 0.7592889609447724 1105 26-27 0.6228936632047066 1105 28-29 0.8011363223614651 1105 30-31 0.9069711411833978 1105 32-33 0.9524131175637152 1105 34-35 1.1227390640669412 1105 36-37 1.1074357712287153 1105 38-39 1.2926870519924663 1105 40-41 1.2747921685224881 1105 42-43 1.2894711508311758 1105 44-45 1.3120394262025243 1105 46-47 1.6613948829288105 1105 48-49 1.4654051177059415 1105 50-51 1.2359644798157632 1105 52-53 1.426032114392907 1105 54-55 1.6454623187009823 1105 56-57 1.8106273242264592 1105 58-59 1.7555866398179347 1105 60-61 2.0153763469539285 1105 62-63 2.0367873264969667 1105 64-65 2.006760086645574 1105 66-67 2.339742509703008 1105 68-69 2.4921759799742986 1105 70-71 2.535349078737198 1105 72-73 2.881111879606877 1105 74-75 3.0707600600533453 1105 76-77 3.1334444660547085 1105 78-79 3.3747405206070553 1105 80-81 3.3575940752858244 1105 82-83 3.4783984505436045 1105 84-85 3.2312137646207866 1105 86-87 3.3085837927926924 1105 88-89 3.5604573836986333 1105 90-91 3.393297036924924 1105 92-93 3.2464935393469467 1105 94-95 3.3600026941007872 1105 96-97 3.1473556418587147 1105 98-99 3.1124461126953626 1105 100-101 2.263077759218074 1106 1 0.17835777289571197 1106 2 0.1108427594188015 1106 3 -0.0520938405675615 1106 4 0.07044890187566466 1106 5 0.0930115228010564 1106 6 -0.15495359852769042 1106 7 0.05090246215372929 1106 8 -0.26307456716885014 1106 9 0.14462483756659594 1106 10-11 0.0529897535074042 1106 12-13 0.1804602896838361 1106 14-15 0.3544278170330841 1106 16-17 -0.16312641871565603 1106 18-19 0.244981298689531 1106 20-21 0.3105765690993181 1106 22-23 0.20945435388909317 1106 24-25 0.3134533790777496 1106 26-27 0.2538857129245571 1106 28-29 0.27946062478104494 1106 30-31 0.013097751852384931 1106 32-33 0.18423134041631073 1106 34-35 0.2792778836218659 1106 36-37 0.20142305840374064 1106 38-39 0.1993289363071611 1106 40-41 0.5032910039659271 1106 42-43 0.16590076687858613 1106 44-45 0.47258974688188715 1106 46-47 0.7191763475216817 1106 48-49 0.6005922291442758 1106 50-51 0.6348338769546231 1106 52-53 0.7056726365475647 1106 54-55 0.45990211784757307 1106 56-57 0.8853432566841803 1106 58-59 0.9783140994797463 1106 60-61 1.2820299570792457 1106 62-63 1.200566758883479 1106 64-65 1.3225414830301325 1106 66-67 1.534582801955544 1106 68-69 1.6686940662096603 1106 70-71 1.4662997019404571 1106 72-73 1.6650314700360482 1106 74-75 1.8619202633141043 1106 76-77 2.0015002584446684 1106 78-79 2.3263279185202776 1106 80-81 2.0490151753253443 1106 82-83 2.1779922600378008 1106 84-85 2.220560228722153 1106 86-87 1.9318354533640765 1106 88-89 1.7040012667173734 1106 90-91 1.9060677888011526 1106 92-93 2.184928018245504 1106 94-95 2.2074370084969512 1106 96-97 2.1616513008011236 1106 98-99 2.1606167257476265 1106 100-101 1.3290888571110386 1107 1 0.26482303508534955 1107 2 0.25303159401275366 1107 3 0.2434597275513397 1107 4 0.1972769431685819 1107 5 0.27794511855447723 1107 6 0.31258172178466026 1107 7 0.292987936966405 1107 8 0.24285685253756384 1107 9 0.32333870454628766 1107 10-11 0.24200797287529952 1107 12-13 0.418604559933371 1107 14-15 0.2983051228927138 1107 16-17 0.4317292257188541 1107 18-19 0.34563965342540115 1107 20-21 0.41688882154823403 1107 22-23 0.4700698152963767 1107 24-25 0.5167350521333063 1107 26-27 0.4295213412569012 1107 28-29 0.5007187114090996 1107 30-31 0.43441242158650084 1107 32-33 0.5943416725699251 1107 34-35 0.6837536857633921 1107 36-37 0.6479118168017308 1107 38-39 0.7799829075684315 1107 40-41 0.9195006909544574 1107 42-43 1.1535002898937847 1107 44-45 0.975908978567638 1107 46-47 0.9163665108870163 1107 48-49 1.0214582546664985 1107 50-51 1.344877329249769 1107 52-53 1.1852257378803621 1107 54-55 1.3796847779203922 1107 56-57 1.590526887005268 1107 58-59 1.4895596240698623 1107 60-61 1.6004048059376288 1107 62-63 1.6756584244959534 1107 64-65 1.6781008467496683 1107 66-67 1.951647609715181 1107 68-69 2.04537476587889 1107 70-71 2.1435183333745798 1107 72-73 2.1759690726971215 1107 74-75 2.4422589578231886 1107 76-77 2.4013359026634333 1107 78-79 2.521110357948409 1107 80-81 2.5977261690207882 1107 82-83 2.658810390951693 1107 84-85 2.6774286038652058 1107 86-87 2.778148088263791 1107 88-89 3.0648923868961653 1107 90-91 3.191266991734455 1107 92-93 3.0675666149844716 1107 94-95 2.776875364782237 1107 96-97 2.370964909479424 1107 98-99 2.0279820515810023 1107 100-101 1.339213751814487 1108 1 0.3166933747637515 1108 2 0.25886646726321416 1108 3 0.3018584285571073 1108 4 0.05449755503521203 1108 5 0.1309534324724808 1108 6 0.21865588344630993 1108 7 0.3589715762517329 1108 8 0.26516008288441384 1108 9 0.2819355983734937 1108 10-11 0.37193086260509034 1108 12-13 0.40713515443244574 1108 14-15 0.38877017553009807 1108 16-17 0.4562435622422285 1108 18-19 0.3936119830176352 1108 20-21 0.4222180555964883 1108 22-23 0.45515363395460895 1108 24-25 0.5748757382148355 1108 26-27 0.36247061762427535 1108 28-29 0.39357305229421513 1108 30-31 0.4300183457025568 1108 32-33 0.5501466347689856 1108 34-35 0.6118559007689797 1108 36-37 0.7886945663675391 1108 38-39 0.7559414038247638 1108 40-41 0.7794606974737164 1108 42-43 0.7063418631263616 1108 44-45 0.770408147082442 1108 46-47 0.9053148327159164 1108 48-49 0.9445190215008594 1108 50-51 1.08169440110494 1108 52-53 1.2532213098305682 1108 54-55 1.4394530595036379 1108 56-57 1.5025062047157434 1108 58-59 1.491422585221919 1108 60-61 1.5098419177769458 1108 62-63 1.6267417135814775 1108 64-65 1.8321704309230107 1108 66-67 2.2741255196682033 1108 68-69 2.4200830689159005 1108 70-71 2.3489812214885717 1108 72-73 2.3634396811780363 1108 74-75 2.5656265172448443 1108 76-77 2.5921089854257495 1108 78-79 2.7767557478404825 1108 80-81 2.9444695069820455 1108 82-83 3.412051338597312 1108 84-85 3.31541136613329 1108 86-87 3.3307101647865807 1108 88-89 3.143441936736007 1108 90-91 3.179377407178176 1108 92-93 3.299906602534378 1108 94-95 3.1886360119256967 1108 96-97 3.3228875059361425 1108 98-99 2.657501107092269 1108 100-101 1.7268124104170788 1109 1 0.7175944870137627 1109 2 0.4220318734623625 1109 3 0.5209281643435908 1109 4 0.5372899563444236 1109 5 0.5390386424140488 1109 6 0.634194333720913 1109 7 0.5843548209364258 1109 8 0.6507891548269669 1109 9 0.72432334733044 1109 10-11 0.6468248812450241 1109 12-13 0.6390248709149091 1109 14-15 0.8294324726677047 1109 16-17 0.8985891609866385 1109 18-19 0.8166206883899676 1109 20-21 0.8007060823581398 1109 22-23 0.9080799672418891 1109 24-25 0.7319172470223023 1109 26-27 0.7712785683660144 1109 28-29 0.9831595264843642 1109 30-31 0.881322991455157 1109 32-33 0.9241876207591346 1109 34-35 1.017168090765118 1109 36-37 0.9719002717790559 1109 38-39 0.9090622983682835 1109 40-41 0.598932626205567 1109 42-43 0.7119560909757112 1109 44-45 1.0457695561524432 1109 46-47 1.1196277816736853 1109 48-49 1.1313072371779498 1109 50-51 1.1314893989703592 1109 52-53 1.1383824757441126 1109 54-55 1.3449350104474966 1109 56-57 1.6058324880930428 1109 58-59 1.4726464056862483 1109 60-61 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2316 40-41 -1.4984176060477452 2316 42-43 -1.2620974741073425 2316 44-45 -1.2610650801258814 2316 46-47 -0.8824427358798985 2316 48-49 -0.5904485308538625 2316 50-51 -0.6333182127250794 2316 52-53 -0.5032149121242426 2316 54-55 -0.5351045034696185 2316 56-57 -0.64632912744527 2316 58-59 -0.20553101222715497 2316 60-61 -0.5317239220320644 2316 62-63 -0.1121641554886601 2316 64-65 -0.1500634737274389 2316 66-67 6.519908429396537E-4 2316 68-69 0.11394437801441626 2316 70-71 -0.1205048408784748 2316 72-73 0.030117942387356322 2316 74-75 -0.11029096645986058 2316 76-77 0.016068281910197157 2316 78-79 0.02198508987956771 2316 80-81 -0.08096125608736315 2316 82-83 -0.22665814592123112 2316 84-85 -0.03282397226971412 2316 86-87 0.17792069650674947 2316 88-89 0.07714851110376131 2316 90-91 0.11749042395031495 2316 92-93 0.2716152434308228 2316 94-95 0.4441528501796821 2316 96-97 0.4600449497397374 2316 98-99 0.2684555198124041 2316 100-101 0.35923260998456996 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6822.0 3 2638.0 4 2818.0 5 3320.0 6 3904.0 7 5063.0 8 6533.0 9 8324.0 10 10579.0 11 13071.0 12 16410.0 13 19708.0 14 22866.0 15 26188.0 16 30713.0 17 35926.0 18 35240.0 19 33864.0 20 36678.0 21 40015.0 22 44361.0 23 49535.0 24 54362.0 25 59773.0 26 65914.0 27 72476.0 28 79179.0 29 87504.0 30 96755.0 31 106643.0 32 118368.0 33 132686.0 34 147804.0 35 158139.0 36 151684.0 37 105071.0 38 25735.0 39 329.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.41775509968968 18.467228661528488 14.352915178155303 22.76210106062653 2 15.460681310022933 19.825615524497053 40.54647774145493 24.167225424025087 3 19.347484289036846 24.77299138930157 29.179731957374926 26.699792364286658 4 12.858516137988332 16.375859661135124 36.654207734001616 34.11141646687493 5 13.810692559931766 37.50074382934832 33.513035544081156 15.175528066638758 6 32.23440413036625 35.71553835265528 17.378269647110894 14.671787869867575 7 29.085203001209624 32.728485295053886 20.066492928538104 18.119818775198375 8 26.6993663074716 34.906840466353444 19.337667888336938 19.056125337838015 9 27.135313631242532 16.723851191168865 19.820738909289776 36.320096268298826 10-11 25.279015265410877 26.689420667925003 26.661058616819034 21.370505449845094 12-13 26.38990023958467 23.973651165454307 27.355257126293708 22.28119146866732 14-15 23.088358337144584 25.227468458359347 26.11740985857104 25.566763345925033 16-17 22.334509044803998 28.58155454796851 26.526529164079584 22.557407243147914 18-19 22.19785472064251 26.653715887454492 29.52730699451653 21.62112239738647 20-21 22.970450941905092 25.835368228911598 29.68079252518648 21.51338830399683 22-23 22.567874911714803 25.79644531901254 29.52494410669731 22.110735662575348 24-25 22.34076085765309 26.06877861774778 29.5964525734707 21.994007951128427 26-27 21.941048160266348 26.452635533916748 29.51304851511499 22.093267790701923 28-29 21.856672144830153 26.66987233591796 29.638177694438493 21.835277824813392 30-31 22.084257183067578 26.22578270010598 29.52922930972314 22.160730807103306 32-33 21.802108064389888 26.355214067677217 29.436640663727204 22.406037204205692 34-35 22.46512311109337 26.424303238408918 29.19681729391712 21.913756356580592 36-37 22.4428349283545 26.104452144146777 29.20417662467778 22.24853630282094 38-39 22.02442410522598 26.401892197736437 29.29525703639512 22.278426660642463 40-41 22.359540567140968 26.28949090767754 29.167011216094707 22.183957309086793 42-43 22.160491395697235 26.259759712366463 29.0524376097075 22.5273112822288 44-45 22.044600006990926 26.632263322013948 28.998873626531285 22.32426304446384 46-47 22.3892071742426 26.853164694996078 28.329132766689458 22.428495364071864 48-49 22.364515210938173 26.77856598197858 28.320881573630423 22.53603723345282 50-51 22.498093801901998 26.96964456155096 28.155282822931348 22.376978813615693 52-53 22.600049039810933 26.937698572106783 28.058101741956687 22.404150646125593 54-55 22.112504007268253 26.910270318773787 28.294952680976582 22.682272992981378 56-57 22.284512891142622 27.114935153013846 28.101934453939336 22.498617501904196 58-59 22.387676233779917 26.72988981247985 28.15593187800809 22.726502075732142 60-61 22.366684369395077 26.849578629850555 28.348789310751748 22.43494769000262 62-63 22.216894963471034 26.898894570621927 28.37758276764541 22.50662769826163 64-65 22.04982051197778 26.735889935576296 28.341761262410394 22.872528290035532 66-67 22.03598940073571 26.96964488186943 28.142356080087623 22.85200963730724 68-69 22.241727307774774 26.679774779733094 28.266625071274714 22.81187284121742 70-71 22.15734983275114 26.793859098804777 28.036871701618544 23.011919366825538 72-73 22.296098435148256 26.8679749268962 27.838422992547457 22.99750364540809 74-75 22.19833955775276 27.003928727289043 27.77879655891382 23.018935156044375 76-77 22.27940973169575 26.8160004924641 27.793181771942173 23.111408003897978 78-79 22.418224393531876 26.907284503907654 27.615490379747033 23.059000722813437 80-81 22.30967540992758 27.09055304714203 27.560483865708562 23.03928767722183 82-83 22.427817469054194 27.04237616200023 27.362842495948847 23.166963872996725 84-85 22.43762688582695 26.674436433249348 27.496757018026884 23.391179662896818 86-87 22.444458703191316 26.705624345314856 27.423404446225046 23.426512505268782 88-89 22.42047710735633 26.730079592674166 27.35226990814665 23.497173391822855 90-91 22.40581660395296 26.769887089237827 27.3453330748779 23.478963231931317 92-93 22.627776259033343 26.400415695423803 27.337139005461218 23.634669040081636 94-95 22.416559315162623 26.282778731723504 27.33711920217093 23.963542750942946 96-97 22.51428929196591 26.16193922192164 27.186907330256776 24.136864155855676 98-99 22.422163344743925 26.19968368934151 26.83736555949241 24.540787406422155 100-101 23.28015729528984 25.2766155920079 27.096145414053108 24.347081698649152 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1152.0 1 847.5 2 699.0 3 859.5 4 717.5 5 658.5 6 1066.0 7 2215.5 8 3511.5 9 3874.0 10 3246.5 11 2551.0 12 2406.5 13 2439.0 14 2582.0 15 2825.0 16 3009.5 17 3271.5 18 3449.5 19 3618.5 20 4050.0 21 4538.0 22 5193.0 23 6211.5 24 7387.0 25 8821.0 26 10764.0 27 13237.5 28 15535.0 29 18030.0 30 21180.5 31 24630.5 32 28405.0 33 31488.0 34 34267.0 35 37181.0 36 40664.5 37 44319.0 38 46721.5 39 48581.0 40 51411.0 41 55136.0 42 58379.5 43 61229.5 44 63979.0 45 66896.5 46 68698.5 47 69951.5 48 72424.0 49 72921.5 50 71151.5 51 68991.0 52 66821.5 53 65932.0 54 65252.0 55 61980.0 56 57056.5 57 52773.0 58 48368.0 59 42770.0 60 36316.5 61 30549.5 62 25401.0 63 20034.5 64 15430.5 65 12029.0 66 9358.5 67 7250.0 68 5543.5 69 4115.0 70 3082.0 71 2400.0 72 1921.0 73 1556.5 74 1247.0 75 984.5 76 721.5 77 480.5 78 291.5 79 191.0 80 137.0 81 74.5 82 39.5 83 27.5 84 15.5 85 12.5 86 9.0 87 5.5 88 4.0 89 5.0 90 5.0 91 2.0 92 1.0 93 1.5 94 0.5 95 1.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.384559608304234 2 0.02832553815914258 3 0.034480995806985715 4 0.043609852488109016 5 0.06447581061639084 6 0.05435582092417415 7 0.03672408630577601 8 0.040114804501621804 9 0.03583728308532404 10-11 0.03677625120109671 12-13 0.029029764245972086 14-15 0.027308322700388837 16-17 0.022326575197261552 18-19 0.02665626150888003 20-21 0.033515945243552676 22-23 0.032524812232459295 24-25 0.04131459709399801 26-27 0.03588944798064474 28-29 0.03137718453540379 30-31 0.033959346853778674 32-33 0.0285863626357461 34-35 0.030177391943027588 36-37 0.03223790530819542 38-39 0.0148669951664008 40-41 0.012702152010591562 42-43 0.013223800963798606 44-45 0.011841431237799935 46-47 0.020422556518055836 48-49 0.017449157484775674 50-51 0.011711018999498174 52-53 0.009807000320292457 54-55 0.008789784861538717 56-57 0.009285351367085411 58-59 0.004381851206939184 60-61 0.008137723670029911 62-63 0.012076173266743106 64-65 0.008189888565350616 66-67 0.003781954910751081 68-69 0.006207622543163842 70-71 0.012702152010591562 72-73 0.010589473750103025 74-75 0.011450194522894652 76-77 0.005581643799315388 78-79 0.009520093396028583 80-81 0.011815348790139584 82-83 0.013562872783383185 84-85 0.00868545507089731 86-87 0.00239958518475241 88-89 0.015727715939192425 90-91 0.025273891782881357 92-93 0.010954628017347957 94-95 0.020709463442319713 96-97 0.01841420804820871 98-99 0.011032875360329014 100-101 0.12428286310157861 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1916998.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.79369155163175 #Duplication Level Percentage of deduplicated Percentage of total 1 79.99865450099969 47.03416217277333 2 11.694330935622117 13.751057718633438 3 3.4964736650514463 6.167117825443145 4 1.5508614621633803 3.6472348178298564 5 0.8309426528740567 2.442709301508595 6 0.48756364817086034 1.7199400045407522 7 0.3485797919107319 1.4346004936712087 8 0.23931712090988297 1.1256269591840178 9 0.18479479381960232 0.9778291297359376 >10 1.0442790096789591 11.427336581019377 >50 0.08046094647544803 3.295242565918687 >100 0.04017169570827751 4.23851874485688 >500 0.00258808804625763 1.0204382385645006 >1k 7.139553231055528E-4 0.5294231708718535 >5k 2.677332461645823E-4 1.1887622754482372 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9859 0.5142937029668263 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7486 0.3905064063707943 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5311 0.27704775904826195 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1940 0.10119989692216685 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.608244766035228E-5 0.0 12-13 0.0 0.0 0.0 5.216489532070456E-5 0.0 14-15 0.0 0.0 0.0 5.216489532070456E-5 0.0 16-17 0.0 0.0 0.0 5.216489532070456E-5 0.0 18-19 0.0 0.0 0.0 7.824734298105684E-5 0.0 20-21 0.0 0.0 0.0 1.5649468596211369E-4 0.0 22-23 0.0 0.0 0.0 2.869069242638751E-4 0.0 24-25 0.0 0.0 0.0 5.998962961881024E-4 0.0 26-27 0.0 0.0 0.0 9.911330110933866E-4 0.0 28-29 0.0 0.0 0.0 0.002686492109016285 0.0 30-31 0.0 0.0 0.0 0.008737619966218014 0.0 32-33 0.0 0.0 0.0 0.022196162958959792 0.0 34-35 0.0 0.0 0.0 0.04374026472641077 0.0 36-37 0.0 0.0 0.0 0.07968187760237622 0.0 38-39 0.0 0.0 0.0 0.1338551213929279 0.0 40-41 0.0 0.0 0.0 0.19149733072230646 0.0 42-43 0.0 0.0 0.0 0.25179994971304087 0.0 44-45 0.0 0.0 0.0 0.3270217287654969 0.0 46-47 0.0 0.0 0.0 0.422170497830462 0.0 48-49 0.0 0.0 0.0 0.5269958549774179 0.0 50-51 5.216489532070456E-5 0.0 0.0 0.6425410981127784 0.0 52-53 5.216489532070456E-5 0.0 0.0 0.7570430433417249 0.0 54-55 5.216489532070456E-5 0.0 0.0 0.8760833344635728 0.0 56-57 5.216489532070456E-5 0.0 0.0 1.0176327779163046 0.0 58-59 5.216489532070456E-5 0.0 0.0 1.1799177672590164 0.0 60-61 5.216489532070456E-5 0.0 0.0 1.3565741852625823 0.0 62-63 5.216489532070456E-5 0.0 0.0 1.534456478306185 0.0 64-65 5.216489532070456E-5 0.0 0.0 1.7133299043608807 0.0 66-67 5.216489532070456E-5 0.0 0.0 1.925458451182526 0.0 68-69 5.216489532070456E-5 0.0 0.0 2.1602265625733565 0.0 70-71 5.216489532070456E-5 0.0 0.0 2.4179733103529584 0.0 72-73 5.216489532070456E-5 0.0 0.0 2.6744159357495416 0.0 74-75 5.216489532070456E-5 0.0 0.0 2.9389441199208344 0.0 76-77 5.216489532070456E-5 0.0 0.0 3.2256945494987477 0.0 78-79 5.216489532070456E-5 0.0 0.0 3.5391012405855404 0.0 80-81 5.216489532070456E-5 0.0 0.0 3.8795554298961186 2.608244766035228E-5 82-83 5.216489532070456E-5 0.0 0.0 4.232581358978987 5.216489532070456E-5 84-85 1.0432979064140912E-4 0.0 0.0 4.5765305962760525 7.824734298105684E-5 86-87 1.0432979064140912E-4 0.0 0.0 4.930104256759789 1.0432979064140912E-4 88-89 1.0432979064140912E-4 0.0 0.0 5.2985709948575845 1.5649468596211369E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4480 0.0 49.412395 1 GTATCAA 7960 0.0 40.875893 1 ATCAACG 10410 0.0 31.088507 3 TATCAAC 10485 0.0 30.775478 2 TCAACGC 10600 0.0 30.531258 4 CAACGCA 10835 0.0 29.781347 5 AACGCAG 11290 0.0 28.701408 6 GTACATG 9230 0.0 25.844269 1 TAGCGCG 150 1.0562708E-6 25.34558 4 TACATGG 9275 0.0 25.208944 2 ACGCAGA 13035 0.0 24.708826 7 TGGTATC 2260 0.0 24.602596 2 CGCAGAG 13120 0.0 24.47633 8 GTGGTAT 2540 0.0 23.666433 1 ACATGGG 9635 0.0 23.527193 3 GCAGAGT 13700 0.0 23.092154 9 GAGTACT 7840 0.0 21.75093 12-13 AGTACTT 8160 0.0 21.18901 12-13 GTGTAGC 1080 0.0 20.762041 1 AGAGTAC 12795 0.0 20.251083 10-11 >>END_MODULE