FastQCFastQC Report
Thu 26 May 2016
SRR938300_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938300_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916998
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96720.5045388675418545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81040.42274431167898974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59750.31168524954120974No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20520.10704236519808576No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50150.047.8405951
GTATCAA87500.040.0161741
TATCAAC114050.030.1120152
ATCAACG114250.030.0161633
TCAACGC115750.029.5451134
CAACGCA117250.029.1266275
AACGCAG121050.028.2122886
GTACATG90300.025.7800921
TACATGG90500.025.0360972
ACGCAGA139400.024.4303997
CGCAGAG140300.024.2398288
ACATGGG93550.023.1015853
GCAGAGT149750.022.2026799
GAGTACT85200.021.5747412-13
AGTACTT87950.021.11616712-13
AGAGTAC137650.020.44496310-11
TAAGGTG9100.020.3562325
TGGTATC25250.020.3169822
GTACTTT91100.020.09926414-15
GCACCGT4300.019.8828326
Produced by FastQC (version 0.11.5)