FastQCFastQC Report
Thu 26 May 2016
SRR938299_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938299_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310946
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39141.2587394595846224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34751.1175573893859383No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33231.068674303576827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23890.7683006052497862No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13120.4219382143523313No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9790.3148456645205277No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9350.30069529757578484No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8540.2746457584275083No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7210.2318730583445357No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5870.18877875901281896No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4380.14086047094993986No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3750.12059971827905809No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG3700.11899172203533734No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.11867012278659317No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3670.11802692428910487No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3520.11320293555794254No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3470.11159493931422175No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.11095174081673345No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC3290.10580615283682698No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT3140.10098216410566466No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG953.4641685E-530.0234959
GTATAGC1354.1620297E-728.1838071
GTAACCC700.00832414827.1816223
GTATCAA45550.025.5812131
GGTATCA33350.025.527051
GACGTGG950.001217819225.023617
GAGTACT30350.024.12101712-13
GTATAAT1000.001638815423.7800871
TACATGG19000.022.7824922
GTATAGA1050.002176563722.6477011
ATACAGG1050.00218268722.636766
GTACATG18950.022.5879461
GTACTTT31800.022.55453514-15
TATCAAC51600.022.4933192
ATTCCGG753.012057E-522.18402510-11
CCGTATC753.0421528E-522.15548592-93
AGTACTT29300.022.1461712-13
ATCAACG52800.021.9821073
TCAACGC52950.021.9198324
CAACGCA53250.021.8856725
Produced by FastQC (version 0.11.5)