##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938299_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 310946 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.285785956404006 31.0 31.0 34.0 30.0 34.0 2 31.330452876062083 33.0 31.0 34.0 30.0 34.0 3 31.32282454188187 33.0 31.0 34.0 28.0 34.0 4 34.976002264058714 37.0 35.0 37.0 33.0 37.0 5 34.98501347500852 37.0 35.0 37.0 33.0 37.0 6 35.04884770989175 37.0 35.0 37.0 32.0 37.0 7 34.99507309950924 37.0 35.0 37.0 32.0 37.0 8 34.9898599756871 37.0 35.0 37.0 32.0 37.0 9 36.50026049539148 39.0 37.0 39.0 32.0 39.0 10-11 36.39689206486014 39.0 37.0 39.0 32.0 39.0 12-13 36.29956005222772 39.0 37.0 39.0 32.0 39.0 14-15 37.2677458465457 40.0 37.0 41.0 32.0 41.0 16-17 37.13894534742367 39.0 37.0 41.0 32.0 41.0 18-19 37.33363188463592 40.0 37.0 41.0 32.0 41.0 20-21 37.359601023972004 40.0 37.0 41.0 32.0 41.0 22-23 37.252212602831364 40.0 37.0 41.0 32.0 41.0 24-25 37.1420873720839 40.0 37.0 41.0 32.0 41.0 26-27 36.92447241643244 39.5 36.0 41.0 31.5 41.0 28-29 36.71725637248912 39.0 36.0 41.0 31.0 41.0 30-31 36.39166929306053 39.0 35.5 40.5 30.5 41.0 32-33 35.99249708952679 38.0 35.0 40.0 30.0 41.0 34-35 35.69084985817473 38.0 35.0 40.0 30.0 41.0 36-37 35.39131392589067 38.0 35.0 40.0 28.5 41.0 38-39 35.120869861648 38.0 35.0 40.0 26.5 41.0 40-41 34.92325677127218 38.0 35.0 40.0 25.5 41.0 42-43 34.511944196098355 38.0 34.5 40.0 23.5 41.0 44-45 33.951615393026444 38.0 33.5 40.0 19.5 41.0 46-47 33.285374309365615 38.0 33.0 40.0 7.5 41.0 48-49 32.724538987476926 38.0 33.0 40.0 2.0 41.0 50-51 31.939331909720657 37.5 32.0 39.5 2.0 40.5 52-53 31.918452078495942 37.5 32.0 39.5 2.0 40.5 54-55 32.00262585786599 38.0 32.0 40.0 2.0 41.0 56-57 31.70292430196883 38.0 31.5 40.0 2.0 41.0 58-59 31.388840827667828 37.0 31.0 40.0 2.0 41.0 60-61 30.976585966695183 37.0 30.5 40.0 2.0 41.0 62-63 30.502270490696134 36.0 29.5 39.5 2.0 41.0 64-65 30.068060049011727 36.0 28.5 39.0 2.0 40.5 66-67 29.59424305184823 35.0 28.0 39.0 2.0 40.0 68-69 29.09478655457861 35.0 27.0 38.5 2.0 40.0 70-71 28.543187563113854 35.0 26.0 38.0 2.0 40.0 72-73 27.964506698912352 34.0 25.5 37.0 2.0 39.0 74-75 27.37344908762293 34.0 24.5 36.5 2.0 39.0 76-77 26.700156940433388 33.5 21.5 36.0 2.0 38.5 78-79 26.03688100184598 33.0 19.0 35.5 2.0 37.5 80-81 25.408357078077866 32.5 15.5 35.0 2.0 37.0 82-83 24.75601551394776 32.0 7.5 35.0 2.0 36.5 84-85 24.12420966984621 31.0 2.0 35.0 2.0 36.0 86-87 23.489229641159557 31.0 2.0 34.5 2.0 35.5 88-89 22.7716259414818 30.5 2.0 34.0 2.0 35.0 90-91 21.974490747589613 29.5 2.0 34.0 2.0 35.0 92-93 21.15598367562213 29.0 2.0 34.0 2.0 35.0 94-95 20.304215201353287 29.0 2.0 34.0 2.0 35.0 96-97 19.220948331864697 26.5 2.0 34.0 2.0 35.0 98-99 17.93225672624829 24.5 2.0 33.0 2.0 35.0 100-101 14.528083654396582 2.0 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8588740273428002 1101 2 0.8118021932202417 1101 3 0.516705924446569 1101 4 0.6732509332899212 1101 5 0.7998886949456647 1101 6 1.030919521959241 1101 7 0.8704172965890464 1101 8 1.05930728262026 1101 9 0.9448010783746383 1101 10-11 1.05870903283175 1101 12-13 1.0017437439222263 1101 14-15 0.9692631457084602 1101 16-17 0.7940400344347722 1101 18-19 0.6319908914966561 1101 20-21 0.9287816575992238 1101 22-23 0.9738649986755021 1101 24-25 0.9014148775836688 1101 26-27 0.8589871111174716 1101 28-29 1.0488299008052593 1101 30-31 0.8282460794218665 1101 32-33 0.8673149057203844 1101 34-35 0.9617601205739632 1101 36-37 1.140558262862129 1101 38-39 1.0740789581349262 1101 40-41 1.2432653508735854 1101 42-43 1.6721982140482012 1101 44-45 1.764034188426173 1101 46-47 1.6312436376269162 1101 48-49 1.465978461818871 1101 50-51 1.2089403163199606 1101 52-53 1.0559297828231458 1101 54-55 0.8914943842883929 1101 56-57 0.6063964026241813 1101 58-59 0.23786451074438375 1101 60-61 0.7065232180074972 1101 62-63 0.44969165696011615 1101 64-65 0.815345902760285 1101 66-67 0.8218797757079557 1101 68-69 0.9627188702577527 1101 70-71 0.8409339112773537 1101 72-73 0.8888086208263317 1101 74-75 1.1734360105362605 1101 76-77 0.7632732944016212 1101 78-79 0.7394288976829912 1101 80-81 0.7995500522134833 1101 82-83 0.7900616169091741 1101 84-85 0.6860503500461057 1101 86-87 0.1558780460196374 1101 88-89 0.20255473972044058 1101 90-91 0.5590278738572074 1101 92-93 0.6965622215554284 1101 94-95 0.7290533893378175 1101 96-97 0.6757886724462701 1101 98-99 0.6541479884301715 1101 100-101 1.141783340396687 1102 1 0.6720127600970152 1102 2 0.4404749065569824 1102 3 0.5515168595513202 1102 4 0.6532160158993605 1102 5 0.5606832784919149 1102 6 0.4374090467420686 1102 7 0.6267513964016871 1102 8 0.8158968755345839 1102 9 0.7208933113843443 1102 10-11 0.8006876139933894 1102 12-13 0.7960132465623175 1102 14-15 1.097217284696704 1102 16-17 1.013870557343985 1102 18-19 0.759242424455266 1102 20-21 0.9305701093110272 1102 22-23 1.1835982212951635 1102 24-25 1.3424704899154634 1102 26-27 1.3302973649072882 1102 28-29 1.0688169132392602 1102 30-31 1.293695960191748 1102 32-33 1.5037748358856078 1102 34-35 0.9917273322925837 1102 36-37 1.3267488607159947 1102 38-39 1.0727163282592613 1102 40-41 1.061045541641775 1102 42-43 1.4954927975944443 1102 44-45 1.4574531119485599 1102 46-47 1.2088124078534506 1102 48-49 1.2913169901786006 1102 50-51 1.2423034571818192 1102 52-53 1.0792222372602076 1102 54-55 0.8980254267002437 1102 56-57 1.7501804683710667 1102 58-59 1.6942763230421143 1102 60-61 1.980321335874983 1102 62-63 2.0511862916049814 1102 64-65 1.8421460645725816 1102 66-67 1.3962921415740794 1102 68-69 0.8909891819593412 1102 70-71 1.4613722948576608 1102 72-73 1.6296534513492382 1102 74-75 1.234312564785963 1102 76-77 1.5119638922554728 1102 78-79 1.6928333432629614 1102 80-81 1.8590905904522828 1102 82-83 1.7438759160064343 1102 84-85 1.1086785224187885 1102 86-87 1.4051115667145808 1102 88-89 1.1326560853174463 1102 90-91 0.8569434759692847 1102 92-93 1.0822397040967324 1102 94-95 1.1707690255156393 1102 96-97 1.367818352228248 1102 98-99 1.5938196797945032 1102 100-101 0.7737934323742053 1103 1 0.4529860763332003 1103 2 0.346165814537688 1103 3 0.08311605354289497 1103 4 0.4879232272323222 1103 5 0.24340905244318378 1103 6 0.3324752981922714 1103 7 0.4521162373371439 1103 8 0.5448460115179756 1103 9 0.7651627135947621 1103 10-11 0.8063815750363119 1103 12-13 0.725334856136719 1103 14-15 0.5614201301507009 1103 16-17 0.5713902495953178 1103 18-19 0.8643746220494535 1103 20-21 0.7525155205585037 1103 22-23 0.6583341314130067 1103 24-25 0.6070793926449056 1103 26-27 0.8595457009883773 1103 28-29 0.7038385899810322 1103 30-31 0.6496524845857223 1103 32-33 0.3385889043963175 1103 34-35 0.4410922953505292 1103 36-37 0.4757535624318052 1103 38-39 0.3049627714417724 1103 40-41 0.5160434044982125 1103 42-43 0.632621087268987 1103 44-45 0.7390217753179229 1103 46-47 0.1621005558659121 1103 48-49 -0.16363507674451938 1103 50-51 -0.30185908785084337 1103 52-53 -0.6294393002619252 1103 54-55 -0.23759159717469913 1103 56-57 -0.33667915182799035 1103 58-59 -0.5539986968027861 1103 60-61 -0.25105498457443076 1103 62-63 -0.666619167270273 1103 64-65 -0.396328625541603 1103 66-67 -0.21308381250786823 1103 68-69 -0.02772303639567042 1103 70-71 -0.31471612182426867 1103 72-73 -0.06220097864404295 1103 74-75 -0.29206589280700257 1103 76-77 -0.41293077709788406 1103 78-79 -0.5260254220786784 1103 80-81 -0.09851764060346468 1103 82-83 -0.15729645988658802 1103 84-85 0.12208146557076915 1103 86-87 0.6346102538978258 1103 88-89 0.47055374667178285 1103 90-91 0.12430013469798595 1103 92-93 -0.12768885424728538 1103 94-95 0.38179668625936714 1103 96-97 0.6630342037273032 1103 98-99 0.8623365021673415 1103 100-101 0.30566740196570485 1104 1 0.2234263279055284 1104 2 0.09773814158171135 1104 3 0.24663806612151262 1104 4 0.3338351769178587 1104 5 0.5452958448960175 1104 6 0.5903369334124022 1104 7 0.345197998343437 1104 8 0.5479906656060223 1104 9 0.5513262356073412 1104 10-11 0.7120419523948058 1104 12-13 0.7143285668285486 1104 14-15 0.6699106961884311 1104 16-17 0.7930883628028553 1104 18-19 0.8690916031815306 1104 20-21 1.0229557721308353 1104 22-23 0.9193404207211913 1104 24-25 0.6117963737769756 1104 26-27 0.6378475877808327 1104 28-29 0.8610712943835495 1104 30-31 1.0836147487366574 1104 32-33 1.0697209798680234 1104 34-35 1.140777829941726 1104 36-37 1.241476832872067 1104 38-39 1.6147111991147298 1104 40-41 1.5380559831145675 1104 42-43 1.4266462445016899 1104 44-45 1.3962544797204401 1104 46-47 1.3837986690734567 1104 48-49 1.0627800175950952 1104 50-51 1.1493987737843767 1104 52-53 1.5089254796122908 1104 54-55 1.330018465718382 1104 56-57 1.9966541815053453 1104 58-59 2.159837781184631 1104 60-61 1.8134104242306037 1104 62-63 2.342814794993874 1104 64-65 2.3300864687980223 1104 66-67 2.172136313278294 1104 68-69 1.8527801082584183 1104 70-71 2.2654725574210133 1104 72-73 2.5195600276452623 1104 74-75 2.1371793902118625 1104 76-77 2.0100251977448806 1104 78-79 1.9063645150282404 1104 80-81 1.6782119191449603 1104 82-83 2.007797879736053 1104 84-85 2.3139053649418386 1104 86-87 2.3295788073569454 1104 88-89 2.0727550045334198 1104 90-91 1.1054322101696847 1104 92-93 1.4650784413501974 1104 94-95 1.2025514032404985 1104 96-97 2.4381914364317048 1104 98-99 2.3639088292113684 1104 100-101 1.612271175550612 1105 1 0.26299495081713076 1105 2 0.1729783314184985 1105 3 0.1741566832454069 1105 4 0.1332525477555322 1105 5 0.03846635074160076 1105 6 0.15135865660567305 1105 7 0.10388920488740183 1105 8 0.12697357257827946 1105 9 0.5686507716261744 1105 10-11 0.5485681707071137 1105 12-13 0.4882125040126297 1105 14-15 0.5513688124827567 1105 16-17 0.5252718716966385 1105 18-19 0.6424546467436727 1105 20-21 0.4644613476218211 1105 22-23 0.40255607068066723 1105 24-25 0.6884063209159237 1105 26-27 0.7316663168003927 1105 28-29 0.5370947538888942 1105 30-31 0.6927419625345479 1105 32-33 0.7444036206057589 1105 34-35 0.9655742955820372 1105 36-37 0.9400819955066311 1105 38-39 0.9358807017577817 1105 40-41 1.2630627354392914 1105 42-43 1.3490427410097752 1105 44-45 1.5004524964662025 1105 46-47 1.4504537603263046 1105 48-49 1.8547216003400138 1105 50-51 2.0983897835463097 1105 52-53 1.727305307524471 1105 54-55 1.9184256887766082 1105 56-57 1.7953191504446515 1105 58-59 1.822028621173832 1105 60-61 1.939823589334587 1105 62-63 1.8303422866017023 1105 64-65 2.4807983567480107 1105 66-67 2.4803799371545168 1105 68-69 2.18135787574694 1105 70-71 1.8383204950681176 1105 72-73 2.1420375520500947 1105 74-75 2.3242573699163067 1105 76-77 2.459652855266974 1105 78-79 2.369332836979087 1105 80-81 2.849093965678126 1105 82-83 2.6115521722853465 1105 84-85 2.5161278443954807 1105 86-87 2.673233551347373 1105 88-89 2.6644129896397217 1105 90-91 2.347512697880454 1105 92-93 3.2071975137737034 1105 94-95 2.6010060854953885 1105 96-97 2.933406932052339 1105 98-99 3.335674465666205 1105 100-101 3.0794548773679526 1106 1 0.27197623819909467 1106 2 -0.08857521974791283 1106 3 0.26676779542495765 1106 4 0.32349429247157246 1106 5 0.22389050982769731 1106 6 -0.07310621636776915 1106 7 0.23130040168721422 1106 8 0.314349767358749 1106 9 0.021407661446737336 1106 10-11 -0.021810889623296248 1106 12-13 -0.07258908069417913 1106 14-15 -0.07583564162036538 1106 16-17 0.13035004928380545 1106 18-19 0.1428440922886125 1106 20-21 0.4872703558338358 1106 22-23 0.2808327020247887 1106 24-25 0.2990905911622832 1106 26-27 0.2928573470521414 1106 28-29 0.6962529745554633 1106 30-31 -0.10601085012770994 1106 32-33 -0.03237677057065724 1106 34-35 -0.3806235619866669 1106 36-37 -0.44690667691678243 1106 38-39 -0.8765892484316495 1106 40-41 -0.10757403206652327 1106 42-43 -0.7331495008503381 1106 44-45 -0.5422374664162675 1106 46-47 -0.7906803435643042 1106 48-49 -0.5008277582768272 1106 50-51 -0.09222698280559527 1106 52-53 -0.2792046834365465 1106 54-55 -0.24313170876556 1106 56-57 -1.1607263906442604 1106 58-59 -0.9499293764029524 1106 60-61 -0.3896809974684956 1106 62-63 -0.8400088875044922 1106 64-65 -0.294861777490496 1106 66-67 -0.9415049792829677 1106 68-69 -0.640708781944852 1106 70-71 -0.6727137953717133 1106 72-73 -0.5069181569331143 1106 74-75 -0.06031770172244322 1106 76-77 -0.09554744475033772 1106 78-79 0.4128016909514294 1106 80-81 1.0572117417036644 1106 82-83 0.19346555190340098 1106 84-85 0.4063522804206343 1106 86-87 0.1795127597632451 1106 88-89 0.24708122049919368 1106 90-91 0.17083904363117242 1106 92-93 0.30165007834473556 1106 94-95 0.2838195170395146 1106 96-97 0.009680563223369631 1106 98-99 -0.2891885071976148 1106 100-101 -0.23165441739905468 1107 1 0.4720417414040732 1107 2 0.2548206535501727 1107 3 0.21429037063667167 1107 4 0.3007646803441233 1107 5 0.13639694317819107 1107 6 -0.05101292108867739 1107 7 0.2775363068011458 1107 8 0.09117099039715981 1107 9 0.2446145112104574 1107 10-11 0.3174629605615209 1107 12-13 0.29655423096071587 1107 14-15 0.26638117398811545 1107 16-17 0.7168797830600653 1107 18-19 0.16329980744324502 1107 20-21 0.2542333465230229 1107 22-23 0.1607512908986024 1107 24-25 0.3870099286441544 1107 26-27 0.26506527293994253 1107 28-29 0.19596756773377422 1107 30-31 0.5408099050306632 1107 32-33 0.6871057541100143 1107 34-35 0.2808979838889627 1107 36-37 0.1545998967977198 1107 38-39 0.6137490288340572 1107 40-41 0.5193945731830922 1107 42-43 0.5682213876538498 1107 44-45 0.6829953039014214 1107 46-47 0.030963786880647604 1107 48-49 -0.2624288437585065 1107 50-51 -0.09041160826762962 1107 52-53 -0.016536831473789704 1107 54-55 -0.12130510510673886 1107 56-57 -0.2017781849641942 1107 58-59 0.006801077908562547 1107 60-61 -0.3533501127073464 1107 62-63 0.12504515263961125 1107 64-65 0.36861082813341994 1107 66-67 -0.13615243167617663 1107 68-69 -0.007489299420171847 1107 70-71 0.4049215389285692 1107 72-73 0.583466908336149 1107 74-75 0.5594876601827643 1107 76-77 0.4786208984864544 1107 78-79 0.08370423154002182 1107 80-81 0.48512546462510997 1107 82-83 0.45668082350776373 1107 84-85 0.14626244745380745 1107 86-87 0.2600021622804398 1107 88-89 0.614161181197268 1107 90-91 -0.11934015442244927 1107 92-93 0.22844556798119342 1107 94-95 0.3688707499034116 1107 96-97 1.2656109426740763 1107 98-99 0.9825749326564086 1107 100-101 0.8626935917713929 1108 1 0.2894640637545862 1108 2 0.30528531139303183 1108 3 0.21519152524100704 1108 4 0.18289178069144185 1108 5 -0.014452582776939948 1108 6 0.14379605290925213 1108 7 0.4929967404818001 1108 8 0.23038060271294114 1108 9 0.10793000919225193 1108 10-11 0.3346834618287673 1108 12-13 0.4643285668285486 1108 14-15 0.566137111282778 1108 16-17 0.9408871049412255 1108 18-19 0.6709783956343642 1108 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547.0 9 681.0 10 904.0 11 1329.0 12 2011.0 13 2687.0 14 3409.0 15 4383.0 16 5721.0 17 7243.0 18 5869.0 19 3841.0 20 3659.0 21 3709.0 22 3819.0 23 4014.0 24 4460.0 25 5018.0 26 5607.0 27 6481.0 28 7528.0 29 8664.0 30 10226.0 31 12294.0 32 14853.0 33 18328.0 34 23058.0 35 29930.0 36 35670.0 37 42733.0 38 24288.0 39 1360.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.078783512581694 21.294962177738896 15.698168064632325 19.928086245047087 2 15.771260071915814 23.221982932066275 39.52472468218941 21.482032313828505 3 19.691643003856875 27.006187744274246 31.870746328240195 21.431422923628684 4 12.359347986362188 19.985061508594463 37.56257907902913 30.093011426014222 5 11.76131459270043 39.38914726684789 34.494531119155894 14.355007021295782 6 27.32063130313302 38.47498382470812 18.273090430343238 15.931294441815623 7 24.175067712320747 36.90015890682399 21.595244375534783 17.329529005320484 8 24.505347808604743 34.34885404101327 20.74949738640933 20.396300763972658 9 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34.205932840082944 18.32410700927137 34-35 18.873394891003024 29.47865834527964 33.58239572925566 18.065551034461677 36-37 18.572894847762367 29.002532199474256 33.81355032384483 18.611022628918555 38-39 18.241285966348574 29.479498236364194 33.86261172508191 18.416604072205324 40-41 18.773052120915764 29.19337482245946 33.54362068494054 18.489952371684236 42-43 18.139763811190498 29.312247268001272 33.26701045188562 19.280978468922612 44-45 18.979044752021792 29.26530395642672 32.67802955379843 19.077621737753056 46-47 19.31781998295354 29.335831336538927 32.120032806394036 19.226315874113503 48-49 18.975307351510427 29.201810740618644 32.1514203700279 19.671461537843033 50-51 18.686881681697134 29.655800131221778 31.916642008979686 19.740676178101403 52-53 19.151519439856813 29.82128872171504 31.254613317552238 19.772578520875918 54-55 18.904693367211557 29.19198822838856 31.22387771675766 20.67944068764222 56-57 19.55067021541777 29.273980203115073 30.79179498725505 20.383554594212107 58-59 19.67345993515516 28.97994827852401 30.912748957850855 20.43384282846997 60-61 19.2362623400101 28.720016469430227 31.56919573210156 20.474525458458114 62-63 19.101185029613678 28.65298364051261 31.4921338412874 20.75369748858631 64-65 18.96489340659871 29.031002703228598 30.99900458149808 21.005099308674613 66-67 19.24006541844163 28.7297597625124 30.588910705756923 21.441264113289048 68-69 19.18938347407542 28.329444230914586 31.010271411639646 21.470900883370348 70-71 19.513164095610787 28.024106966218397 30.961721264409412 21.501007673761396 72-73 19.32782300025252 27.52480534499092 31.516177958346 21.631193696410563 74-75 18.918340758850306 28.48326443954771 30.763353867434418 21.835040934167566 76-77 19.209521129025774 27.723211772747376 30.838647420690762 22.228619677536084 78-79 19.23336688847365 27.810511013697575 30.627373918972378 22.328748178856404 80-81 19.437882620448555 27.487726396328448 30.465070280753153 22.609320702469837 82-83 19.401285807553396 27.4652417080207 30.76128940961558 22.37218307481033 84-85 19.493843499291643 27.282054085867784 30.4251901142213 22.798912300619268 86-87 19.41774427771247 27.580195650530776 30.037969004041344 22.96409106771541 88-89 19.931027161272265 27.05736262534301 29.885924586693775 23.125685626690945 90-91 20.303529922710055 26.709346373163257 29.87815372736997 23.10896997675672 92-93 20.49906228788888 26.594512695303074 30.051823472076123 22.854601544731924 94-95 20.402362180657597 26.300413912478344 29.98476583299685 23.31245807386721 96-97 20.553654703362497 26.272136616847718 29.715065205396595 23.459143474393187 98-99 21.05699111855967 26.057747054263313 29.178153136489822 23.707108690687193 100-101 21.562683041432535 25.293670869780705 29.71900927517202 23.424636813614743 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2395.0 1 1401.5 2 443.5 3 442.5 4 361.5 5 371.0 6 588.0 7 1005.0 8 1349.0 9 1385.5 10 1053.0 11 715.0 12 646.5 13 629.0 14 609.0 15 647.0 16 718.0 17 769.0 18 880.0 19 995.0 20 1097.0 21 1312.0 22 1535.0 23 1735.5 24 2025.5 25 2435.0 26 3045.5 27 3795.5 28 4321.5 29 4823.0 30 5460.0 31 6113.0 32 6708.5 33 7252.5 34 8261.0 35 9524.0 36 10421.5 37 10962.0 38 10698.5 39 10131.5 40 10119.5 41 10215.0 42 10152.0 43 10285.5 44 10504.0 45 10733.5 46 11042.0 47 11784.0 48 13181.0 49 12929.0 50 11221.5 51 9822.0 52 8618.5 53 7590.5 54 6568.0 55 5572.0 56 4660.0 57 3865.0 58 3146.5 59 2462.5 60 1914.5 61 1435.5 62 1041.0 63 779.5 64 608.5 65 490.0 66 402.0 67 345.5 68 267.5 69 222.5 70 192.0 71 159.0 72 143.0 73 121.0 74 100.0 75 91.0 76 80.0 77 66.5 78 49.0 79 31.0 80 24.0 81 16.0 82 11.5 83 8.0 84 4.0 85 1.0 86 0.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005788786477394789 2 0.09808777086696727 3 0.10677095058305944 4 0.10902214532426853 5 0.14857885291979958 6 0.09229898438957247 7 0.023155145909579157 8 0.022833546660835 9 0.020582351919625916 10-11 0.04823988731162324 12-13 0.050330282428460243 14-15 0.02411994365581162 16-17 0.031195127128183026 18-19 0.034571919239996654 20-21 0.057244666276459584 22-23 0.031677526001299264 24-25 0.046310291819158314 26-27 0.05515427115962258 28-29 0.052742276794041414 30-31 0.06544544711943553 32-33 0.048400686935995314 34-35 0.05804866439831996 36-37 0.04791828806287909 38-39 0.02653193802139278 40-41 0.032642323747531725 42-43 0.02990873013320641 44-45 0.006914383847999331 46-47 0.009969576711068802 48-49 0.007557582345487641 50-51 0.007075183472371409 52-53 0.00820078084297595 54-55 0.009165578589208415 56-57 0.012381571076649965 58-59 0.016079962437207745 60-61 0.02154714966585838 62-63 0.00820078084297595 64-65 0.006914383847999331 66-67 0.007879181594231795 68-69 0.011095174081673344 70-71 0.026210338772648625 72-73 0.0242807432801837 74-75 0.02604953914827655 76-77 0.018813556051533065 78-79 0.004823988731162324 80-81 0.004663189106790247 82-83 0.0041807902336740135 84-85 0.004663189106790247 86-87 0.011095174081673344 88-89 0.031838325625671335 90-91 0.03328552224502004 92-93 0.02669273764576486 94-95 0.04229030120985637 96-97 0.03875270947367067 98-99 0.02347674515832331 100-101 0.0720382317186907 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 310946.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.5627624563235 #Duplication Level Percentage of deduplicated Percentage of total 1 79.26526518107889 48.79788691380249 2 12.509625491604574 15.40254205114443 3 4.1390608231720005 7.64436054747638 4 1.672638915525618 4.118890889268247 5 0.83672341265718 2.5755502347529156 6 0.4501365474598393 1.6626989606507807 7 0.2632410658428535 1.1344093043663102 8 0.1692622249549091 0.8336200118182289 9 0.10036081008505238 0.5560639840071246 >10 0.5073469644107484 5.5952663420856945 >50 0.05127987390555102 2.127062590401698 >100 0.02982604910833056 3.5956796356298155 >500 0.002616320097221979 1.3130254609234122 >1k 0.002616320097221979 4.642943073672508 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3914 1.2587394595846224 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3475 1.1175573893859383 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3323 1.068674303576827 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2389 0.7683006052497862 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1312 0.4219382143523313 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 979 0.3148456645205277 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 935 0.30069529757578484 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 854 0.2746457584275083 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 721 0.2318730583445357 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 587 0.18877875901281896 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 438 0.14086047094993986 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 375 0.12059971827905809 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 370 0.11899172203533734 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 369 0.11867012278659317 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 367 0.11802692428910487 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 352 0.11320293555794254 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 347 0.11159493931422175 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 345 0.11095174081673345 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 329 0.10580615283682698 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 314 0.10098216410566466 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.6079962437207747E-4 0.0 12-13 0.0 0.0 0.0 3.2159924874415493E-4 0.0 14-15 0.0 0.0 0.0 3.2159924874415493E-4 0.0 16-17 0.0 0.0 0.0 4.823988731162324E-4 0.0 18-19 0.0 0.0 0.0 6.431984974883099E-4 0.0 20-21 0.0 0.0 0.0 0.001447196619348697 0.0 22-23 0.0 0.0 0.0 0.005627986853022711 0.0 24-25 0.0 0.0 0.0 0.013667968071626586 0.0 26-27 0.0 0.0 0.0 0.026853537270136937 0.0 28-29 0.0 0.0 0.0 0.05740546590083166 0.0 30-31 0.0 0.0 0.0 0.12397651039087174 0.0 32-33 0.0 0.0 0.0 0.2370186463244422 0.0 34-35 0.0 0.0 0.0 0.4302997948196793 0.0 36-37 0.0 0.0 0.0 0.7092871431052337 0.0 38-39 0.0 0.0 0.0 1.0278311989863191 0.0 40-41 0.0 0.0 0.0 1.3129289329980125 0.0 42-43 0.0 0.0 0.0 1.6121770339544486 0.0 44-45 0.0 0.0 0.0 1.9890913534825982 0.0 46-47 0.0 0.0 0.0 2.4063663787281393 0.0 48-49 0.0 0.0 0.0 2.791159879850521 0.0 50-51 0.0 0.0 0.0 3.199108526882481 0.0 52-53 0.0 0.0 0.0 3.555762093739749 0.0 54-55 0.0 0.0 0.0 3.9876698848031493 0.0 56-57 0.0 0.0 0.0 4.498208692184495 0.0 58-59 0.0 0.0 0.0 4.998938722479144 0.0 60-61 0.0 0.0 0.0 5.528612685160768 0.0 62-63 0.0 0.0 0.0 6.021624333485557 0.0 64-65 0.0 0.0 0.0 6.548886301801599 0.0 66-67 0.0 0.0 0.0 7.179864027837631 0.0 68-69 3.2159924874415493E-4 0.0 0.0 7.87017681526696 0.0 70-71 3.2159924874415493E-4 0.0 0.0 8.596830317804379 0.0 72-73 3.2159924874415493E-4 0.0 0.0 9.298559878564124 0.0 74-75 3.2159924874415493E-4 0.0 0.0 10.026338978472147 0.0 76-77 3.2159924874415493E-4 0.0 0.0 10.789976394615142 0.0 78-79 3.2159924874415493E-4 0.0 0.0 11.63819441317785 0.0 80-81 3.2159924874415493E-4 0.0 0.0 12.527577135579811 0.0 82-83 3.2159924874415493E-4 0.0 0.0 13.359554392080941 0.0 84-85 3.2159924874415493E-4 0.0 0.0 14.227229165192671 0.0 86-87 3.2159924874415493E-4 0.0 0.0 15.119023881960212 0.0 88-89 3.2159924874415493E-4 0.0 0.0 16.07706804396905 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 95 3.4641685E-5 30.023495 9 GTATAGC 135 4.1620297E-7 28.183807 1 GTAACCC 70 0.008324148 27.181622 3 GTATCAA 4555 0.0 25.581213 1 GGTATCA 3335 0.0 25.52705 1 GACGTGG 95 0.0012178192 25.02361 7 GAGTACT 3035 0.0 24.121017 12-13 GTATAAT 100 0.0016388154 23.780087 1 TACATGG 1900 0.0 22.782492 2 GTATAGA 105 0.0021765637 22.647701 1 ATACAGG 105 0.002182687 22.63676 6 GTACATG 1895 0.0 22.587946 1 GTACTTT 3180 0.0 22.554535 14-15 TATCAAC 5160 0.0 22.493319 2 ATTCCGG 75 3.012057E-5 22.184025 10-11 CCGTATC 75 3.0421528E-5 22.155485 92-93 AGTACTT 2930 0.0 22.14617 12-13 ATCAACG 5280 0.0 21.982107 3 TCAACGC 5295 0.0 21.919832 4 CAACGCA 5325 0.0 21.885672 5 >>END_MODULE