##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938299_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 310946 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.581975648504883 31.0 31.0 34.0 30.0 34.0 2 31.800074611025707 31.0 31.0 34.0 30.0 34.0 3 31.812160310793512 31.0 31.0 34.0 30.0 34.0 4 35.555228238986835 37.0 35.0 37.0 33.0 37.0 5 35.46207701658809 37.0 35.0 37.0 33.0 37.0 6 35.48497488309867 37.0 35.0 37.0 33.0 37.0 7 35.409122484289874 37.0 35.0 37.0 33.0 37.0 8 35.43520418336303 37.0 35.0 37.0 33.0 37.0 9 36.918490670405795 39.0 37.0 39.0 33.0 39.0 10-11 36.84074726801438 39.0 37.0 39.0 32.0 39.0 12-13 36.73653303145884 39.0 36.0 39.0 32.0 39.0 14-15 37.618633138872994 39.0 36.5 41.0 32.0 41.0 16-17 37.53688421783846 39.0 36.0 41.0 32.0 41.0 18-19 37.831744097045785 39.5 37.0 41.0 32.5 41.0 20-21 37.87045017462839 40.0 37.0 41.0 32.5 41.0 22-23 37.73343924668592 40.0 36.5 41.0 32.0 41.0 24-25 37.55951676496883 39.0 36.0 41.0 32.0 41.0 26-27 37.299207901050345 39.0 36.0 41.0 31.5 41.0 28-29 37.14324673737562 39.0 36.0 41.0 31.5 41.0 30-31 36.76679712876191 39.0 35.0 40.0 31.0 41.0 32-33 36.38868967602092 38.0 35.0 40.0 30.0 41.0 34-35 36.08480893788632 38.0 35.0 40.0 30.0 41.0 36-37 35.90663813009333 38.0 35.0 40.0 30.0 41.0 38-39 35.608509194522526 38.0 35.0 40.0 29.0 41.0 40-41 35.35968624777293 38.0 35.0 40.0 27.5 41.0 42-43 34.97818592295768 38.0 34.0 40.0 26.5 41.0 44-45 34.448569848140835 38.0 33.5 40.0 24.0 41.0 46-47 33.76122542177741 38.0 33.0 40.0 20.5 41.0 48-49 33.17392569770957 38.0 33.0 40.0 12.0 41.0 50-51 32.658514661709745 37.5 32.5 40.0 6.5 41.0 52-53 32.15631974683707 37.0 31.0 40.0 2.0 41.0 54-55 31.761199693837515 37.0 31.0 40.0 2.0 41.0 56-57 31.32813575347488 36.0 30.5 39.5 2.0 41.0 58-59 30.762416946994012 36.0 29.0 39.0 2.0 40.0 60-61 30.274687566329845 35.5 28.0 39.0 2.0 40.0 62-63 29.759463057894294 35.0 28.0 39.0 2.0 40.0 64-65 29.197069587645444 35.0 26.5 38.0 2.0 40.0 66-67 28.629104410412097 34.0 26.0 38.0 2.0 40.0 68-69 28.112513105169384 34.0 25.5 37.5 2.0 39.5 70-71 27.595786728242203 34.0 24.5 37.0 2.0 39.0 72-73 27.132556778347364 33.0 22.5 36.0 2.0 39.0 74-75 26.569412373852693 33.0 22.0 36.0 2.0 38.5 76-77 25.397763598824234 31.0 20.5 34.5 2.0 36.5 78-79 26.023261273661667 32.0 21.0 35.0 2.0 37.0 80-81 25.641514925421134 32.0 20.0 35.0 2.0 37.0 82-83 25.216023039370178 32.0 18.0 35.0 2.0 36.0 84-85 24.68388241045069 31.5 13.0 35.0 2.0 36.0 86-87 24.10579168087063 31.0 7.0 34.0 2.0 35.0 88-89 23.421888366468778 31.0 2.0 34.0 2.0 35.0 90-91 22.848782425244252 30.0 2.0 34.0 2.0 35.0 92-93 22.087999202433863 29.5 2.0 34.0 2.0 35.0 94-95 21.356449351334312 29.0 2.0 34.0 2.0 35.0 96-97 20.375256475400874 29.0 2.0 34.0 2.0 35.0 98-99 19.149834697986144 26.5 2.0 33.5 2.0 35.0 100-101 15.77325001768796 13.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3703000051688008 1101 2 0.3014980158782734 1101 3 0.09609700002143384 1101 4 0.07141995757485375 1101 5 -0.08709558599093725 1101 6 0.3071603822813529 1101 7 0.3882495892762705 1101 8 0.24015206452499172 1101 9 0.19972257626611878 1101 10-11 0.22466559499048344 1101 12-13 0.4981730975869141 1101 14-15 0.58069038837764 1101 16-17 0.4364622564138543 1101 18-19 0.6575513426238118 1101 20-21 0.6381127267911424 1101 22-23 0.4014094348318764 1101 24-25 0.6792583746621546 1101 26-27 0.32279411512093503 1101 28-29 0.1411661210811772 1101 30-31 0.4600518737283892 1101 32-33 -0.01772733813088223 1101 34-35 -0.1411325117895288 1101 36-37 0.1057158870748296 1101 38-39 -0.20126838778439549 1101 40-41 -0.4731088899776168 1101 42-43 0.07545166328018382 1101 44-45 -0.14705768764889626 1101 46-47 -0.0330974779391795 1101 48-49 0.5352705950800285 1101 50-51 0.6013020201748844 1101 52-53 0.26325727089240303 1101 54-55 0.3904492241815909 1101 56-57 0.16264281106833423 1101 58-59 0.6553990929854656 1101 60-61 0.5259557245509008 1101 62-63 0.05371684371304397 1101 64-65 0.4146739693661381 1101 66-67 0.3682471742155613 1101 68-69 0.8565837831427814 1101 70-71 0.9232772390485842 1101 72-73 0.9537154128387364 1101 74-75 0.7866119687963362 1101 76-77 0.600218859318737 1101 78-79 0.8716558795451981 1101 80-81 0.6714409912774677 1101 82-83 0.7294875684403088 1101 84-85 0.7409610047460262 1101 86-87 0.4811811780049098 1101 88-89 0.5170325808897793 1101 90-91 0.9162431020337571 1101 92-93 1.1744662203246712 1101 94-95 0.9153601096827337 1101 96-97 1.0259719459585277 1101 98-99 1.4610242182552362 1101 100-101 1.2283078703087877 1102 1 0.380700703516613 1102 2 0.4778414837713072 1102 3 0.3628530892736883 1102 4 0.3657799473551293 1102 5 0.21756929222402022 1102 6 0.187855749339775 1102 7 0.016251633221081363 1102 8 0.34266867157659675 1102 9 0.3950576980511613 1102 10-11 0.33212173534135303 1102 12-13 0.3924255673535626 1102 14-15 0.8136628377048467 1102 16-17 0.5891513213091031 1102 18-19 0.3487826064630184 1102 20-21 0.567048385111697 1102 22-23 0.40302223503626067 1102 24-25 0.4300567906049224 1102 26-27 0.5219509878853685 1102 28-29 0.5655774223927068 1102 30-31 0.5052688299538985 1102 32-33 0.5543802670471081 1102 34-35 0.856536113657576 1102 36-37 0.8888874081104277 1102 38-39 0.4055596653581759 1102 40-41 0.8995171532858777 1102 42-43 0.37708256091260495 1102 44-45 0.5142329783364517 1102 46-47 0.6227320229976314 1102 48-49 0.9022544990146173 1102 50-51 1.1530127594015909 1102 52-53 1.1439952201344354 1102 54-55 0.7681531928411012 1102 56-57 1.1804687776839025 1102 58-59 1.4776770268979185 1102 60-61 1.2621058693303269 1102 62-63 0.9654439770804437 1102 64-65 1.29158484485583 1102 66-67 1.2304139686287812 1102 68-69 1.6710851457726577 1102 70-71 1.130370366284147 1102 72-73 1.5748031747191682 1102 74-75 2.087215571249068 1102 76-77 2.0045633066019946 1102 78-79 2.4560697357877466 1102 80-81 2.1001477703610973 1102 82-83 1.8661018590211285 1102 84-85 1.4600197255772294 1102 86-87 1.1939962861636566 1102 88-89 1.5639847610975757 1102 90-91 2.1826053060875807 1102 92-93 2.5614743961039252 1102 94-95 2.276877525795829 1102 96-97 1.881804640559107 1102 98-99 2.3734805715170317 1102 100-101 2.0358310775988553 1103 1 0.38790593035913545 1103 2 0.4762538914161709 1103 3 0.45835287555933135 1103 4 0.2584648102726348 1103 5 0.25503036568069604 1103 6 0.25425985265540163 1103 7 0.5264488610405849 1103 8 0.3524079400628892 1103 9 0.6217475679907096 1103 10-11 0.3667129303099088 1103 12-13 0.5853027731910174 1103 14-15 0.7431676310125326 1103 16-17 0.8059798002734979 1103 18-19 0.6897943912832005 1103 20-21 0.750109995907323 1103 22-23 0.6721496698533898 1103 24-25 0.7191665176611579 1103 26-27 0.6394938834095782 1103 28-29 0.7518600138319229 1103 30-31 0.617667315635039 1103 32-33 0.5551893285357821 1103 34-35 0.30944262889236285 1103 36-37 0.8226970191503042 1103 38-39 0.6915857995707881 1103 40-41 0.8359981937694769 1103 42-43 0.8527191243857715 1103 44-45 0.5725922626986772 1103 46-47 0.22861222083606236 1103 48-49 0.343177745030367 1103 50-51 0.17910117608352039 1103 52-53 0.23311410637733587 1103 54-55 0.28129662570426106 1103 56-57 -0.16590113133484508 1103 58-59 -0.6507805660678301 1103 60-61 -0.3381414287096085 1103 62-63 -0.21978530789238349 1103 64-65 0.13323777274250048 1103 66-67 0.10329972304045398 1103 68-69 0.5122648490150077 1103 70-71 0.27068910598006113 1103 72-73 0.5806497888731634 1103 74-75 0.08718504062685639 1103 76-77 -0.19903014763260174 1103 78-79 0.4569856544541686 1103 80-81 0.14224949839431744 1103 82-83 0.22822556910234226 1103 84-85 0.6467144804163318 1103 86-87 0.3410164974355645 1103 88-89 -0.4561654495637164 1103 90-91 0.027112847151268937 1103 92-93 0.1667287161869666 1103 94-95 0.03292258899422151 1103 96-97 0.13687272384663984 1103 98-99 0.33195892530588367 1103 100-101 0.23795906196717986 1104 1 0.14262291149120898 1104 2 0.0643042058815837 1104 3 0.1659000453706554 1104 4 -0.14405091299780537 1104 5 -0.08459227582874007 1104 6 -0.13253260017478397 1104 7 0.10820986732989724 1104 8 0.2549236633333294 1104 9 0.1374708384309642 1104 10-11 0.1402978359702871 1104 12-13 0.2912776159583075 1104 14-15 0.38467706497480236 1104 16-17 0.203778542411861 1104 18-19 0.37690130952218936 1104 20-21 0.34916659968090613 1104 22-23 0.4064263994131352 1104 24-25 0.5021853855856904 1104 26-27 0.28257564441587135 1104 28-29 0.19053925911493508 1104 30-31 0.3928245483394406 1104 32-33 0.5394660580955346 1104 34-35 0.2638451446156296 1104 36-37 0.39345173613143203 1104 38-39 -0.019106024328586102 1104 40-41 0.4586397032034384 1104 42-43 0.48164994199583333 1104 44-45 0.5395733947741448 1104 46-47 0.5635178812134214 1104 48-49 0.6623601349674786 1104 50-51 1.1067741320583622 1104 52-53 1.3651895780754515 1104 54-55 1.3048815313646394 1104 56-57 1.3089416359607533 1104 58-59 1.6589678616051238 1104 60-61 1.8112296404727815 1104 62-63 1.901283874497551 1104 64-65 1.4870113576481643 1104 66-67 1.2495261381347937 1104 68-69 1.3723277420967719 1104 70-71 1.6197457097536478 1104 72-73 1.6105240027096421 1104 74-75 1.4755498205010653 1104 76-77 1.330844066203877 1104 78-79 1.9711365978503927 1104 80-81 2.0762117625452596 1104 82-83 2.2109299716180644 1104 84-85 1.6294188829320575 1104 86-87 1.867746057183993 1104 88-89 1.9825137957193029 1104 90-91 1.786546809415416 1104 92-93 1.9670431815957699 1104 94-95 2.356821960063403 1104 96-97 2.3224073150416125 1104 98-99 2.852399176878208 1104 100-101 2.385757804105543 1105 1 0.47134537165249313 1105 2 0.46639549731191465 1105 3 0.39021227286611904 1105 4 0.44752990268302284 1105 5 0.38781965456443857 1105 6 0.4482444959397327 1105 7 0.36564707070991176 1105 8 0.37322439258440454 1105 9 -0.008321498645088354 1105 10-11 0.3556391177544427 1105 12-13 0.34579781505543394 1105 14-15 0.4719460390072854 1105 16-17 0.6030145650996772 1105 18-19 0.4210422208931064 1105 20-21 0.2705695398360177 1105 22-23 0.2934117758070087 1105 24-25 0.4264532406615089 1105 26-27 0.3518544961348198 1105 28-29 0.5983121494957686 1105 30-31 0.857220504661548 1105 32-33 0.8517128459182004 1105 34-35 0.7292057187561554 1105 36-37 1.019826316522682 1105 38-39 0.9283126583893235 1105 40-41 0.9419903996575059 1105 42-43 0.7555049406453733 1105 44-45 0.741564365951362 1105 46-47 1.531521508176418 1105 48-49 2.3148758582379187 1105 50-51 1.9442649681890884 1105 52-53 2.0321263377312775 1105 54-55 1.931940990406968 1105 56-57 1.9188887749042998 1105 58-59 1.9240158995724848 1105 60-61 1.8887463283610373 1105 62-63 1.9852635017692286 1105 64-65 2.037827424322547 1105 66-67 1.6246949462530154 1105 68-69 1.4801913065525305 1105 70-71 1.9768472261328611 1105 72-73 2.1605587289511057 1105 74-75 1.5863072384120898 1105 76-77 1.4015891370068232 1105 78-79 1.7737082689543051 1105 80-81 1.8241088957011016 1105 82-83 1.523784468666335 1105 84-85 2.004250074813836 1105 86-87 2.0877560892093 1105 88-89 2.1107018576091825 1105 90-91 2.523746330964979 1105 92-93 2.4360500110899252 1105 94-95 2.0022284500120833 1105 96-97 2.7283956624583645 1105 98-99 3.5026673406317315 1105 100-101 2.9356960685651625 1106 1 -0.0853694388173416 1106 2 0.15877198057056674 1106 3 0.02488350389868188 1106 4 0.24656638555703836 1106 5 -0.0889001561938727 1106 6 -0.15552110592191326 1106 7 0.012569008514020652 1106 8 -0.007393719999015502 1106 9 -0.2639900949102554 1106 10-11 0.2532293633455396 1106 12-13 -0.15081737428241127 1106 14-15 0.10095828971405041 1106 16-17 0.07452734468311206 1106 18-19 -0.1252969909845021 1106 20-21 0.21059973388567954 1106 22-23 0.24151935893680587 1106 24-25 0.17922743917296202 1106 26-27 0.1679929271981493 1106 28-29 0.2428252944849092 1106 30-31 0.11025914150598481 1106 32-33 -0.09345225976097993 1106 34-35 -0.03975691370340684 1106 36-37 0.1198224701469286 1106 38-39 0.11857363498412354 1106 40-41 0.18966334232727888 1106 42-43 0.2058963093781827 1106 44-45 0.28559575237750323 1106 46-47 -0.5833985833673268 1106 48-49 0.07663134413133577 1106 50-51 -0.05407147429798442 1106 52-53 0.11417914847035604 1106 54-55 0.5115513525432469 1106 56-57 -0.0520133925284334 1106 58-59 -0.23252678558242934 1106 60-61 0.019407712671672073 1106 62-63 -0.3924554050907858 1106 64-65 0.328142250177887 1106 66-67 0.12131494356055583 1106 68-69 0.24073037611423587 1106 70-71 0.36520616991807486 1106 72-73 0.8933654293376669 1106 74-75 1.1303722806260623 1106 76-77 1.2344737265023689 1106 78-79 1.1498742016042662 1106 80-81 0.11160144334808564 1106 82-83 1.0700563689682738 1106 84-85 0.6377241213934433 1106 86-87 1.0557253951307999 1106 88-89 1.6080190105218506 1106 90-91 1.2764868736886008 1106 92-93 0.9307564364606193 1106 94-95 1.1743778047825515 1106 96-97 1.4737992434341898 1106 98-99 1.6031779470128669 1106 100-101 1.8037749961956049 1107 1 0.004107939182940612 1107 2 0.09504671972744916 1107 3 0.09207107700493466 1107 4 0.13749419166754961 1107 5 0.3531341862007409 1107 6 0.3371191411187482 1107 7 -0.0251046919491813 1107 8 0.4413500221055102 1107 9 0.041582356313249136 1107 10-11 0.27590282986871983 1107 12-13 0.0987966246882479 1107 14-15 0.39032805530616344 1107 16-17 0.35175094455211564 1107 18-19 0.3413150896780195 1107 20-21 0.2687244707031553 1107 22-23 0.3577969053739025 1107 24-25 0.19949600231337428 1107 26-27 0.35158437435109846 1107 28-29 0.06381439288007584 1107 30-31 0.19177320130105358 1107 32-33 0.2847913185855333 1107 34-35 0.481952719946527 1107 36-37 0.02511887214945574 1107 38-39 0.23801872926195955 1107 40-41 0.12085222549763586 1107 42-43 0.03116651009872129 1107 44-45 0.2296606941264372 1107 46-47 0.2777626948809342 1107 48-49 0.22084600749165162 1107 50-51 0.08807048050481114 1107 52-53 -0.665346421173588 1107 54-55 -0.9301320798246984 1107 56-57 -0.9610855864179193 1107 58-59 -0.5337947404787933 1107 60-61 -0.32474144748366385 1107 62-63 -0.558262732092139 1107 64-65 -0.5295473582065249 1107 66-67 -0.3692479161222231 1107 68-69 -0.26693349886821593 1107 70-71 0.021632502206479387 1107 72-73 -0.38694149996566196 1107 74-75 -0.9241451011172543 1107 76-77 -0.627712209023759 1107 78-79 -0.2414185509339859 1107 80-81 -0.02701737478694355 1107 82-83 -0.0620776318739118 1107 84-85 -0.20926974937809106 1107 86-87 -0.1578899139039649 1107 88-89 -0.25594954793976044 1107 90-91 -0.3852592546041045 1107 92-93 -0.14126433741295585 1107 94-95 -0.25181879840839017 1107 96-97 0.19105933794595487 1107 98-99 0.10008058117746899 1107 100-101 0.2048838718798791 1108 1 0.07344052155410452 1108 2 0.03285766500107812 1108 3 0.13445350449015336 1108 4 -0.05914525262044634 1108 5 0.0443385417813289 1108 6 0.11275041869313895 1108 7 0.02330420695253821 1108 8 0.19203058157232533 1108 9 0.3890431654749875 1108 10-11 0.19532928251116743 1108 12-13 0.06486252161869288 1108 14-15 0.17555756811945145 1108 16-17 0.022960932348972563 1108 18-19 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581.0 10 1002.0 11 1588.0 12 2100.0 13 2915.0 14 3558.0 15 4780.0 16 6003.0 17 7378.0 18 6273.0 19 4611.0 20 4345.0 21 4426.0 22 4472.0 23 4787.0 24 5191.0 25 5539.0 26 5895.0 27 6789.0 28 7885.0 29 9222.0 30 11133.0 31 12961.0 32 15115.0 33 18490.0 34 23328.0 35 30464.0 36 36899.0 37 42927.0 38 19160.0 39 470.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.049381723455916 22.651594333403015 16.167489427390695 20.131534515750374 2 15.808524261036592 23.77842197891296 38.82409340844962 21.588960351600832 3 19.36767155711924 27.41247676445428 32.653258121989026 20.56659355643745 4 12.275443324564392 21.194355289986042 36.46195802486606 30.06824336058351 5 11.163947453935325 40.4555150524592 34.55357401395695 13.826963479648535 6 26.870582030320378 39.110649437522916 17.693104268908428 16.32566426324828 7 23.34360306934323 37.31902002276923 21.048670830304943 18.288706077582603 8 24.576936188277067 35.309667916615744 20.261074270130504 19.852321624976685 9 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34.08984561553589 18.258563827306805 34-35 18.321025614635463 29.998134544378548 33.77117935390909 17.909660487076895 36-37 18.573333311888728 29.159784288717294 33.69708744734679 18.569794952047182 38-39 18.079560292537966 29.330018589153966 33.85348659200988 18.736934526298185 40-41 18.428326780674883 29.393215787484824 33.649147350394365 18.529310081445928 42-43 18.27964424176252 29.25485983697898 33.339711810742536 19.125784110515966 44-45 18.718300029267695 29.40293899132583 32.62045586849477 19.258305110911706 46-47 19.45016176442212 29.027708991271794 31.978703697748163 19.543425546557923 48-49 19.004746804911463 29.037678567982866 32.02951637904974 19.928058248055933 50-51 18.51886989660551 29.478525141102125 32.04425219894193 19.958352763350433 52-53 19.0742122426402 29.76304567352531 31.321354833315105 19.841387250519382 54-55 18.728482827266046 29.180542445814112 31.08458610647915 21.006388620440692 56-57 19.58716304438713 29.60948203224997 30.223575797726937 20.579779125635962 58-59 19.337531060770548 29.931565784500574 30.29778131609209 20.433121838636783 60-61 18.960526908209143 30.195918262334935 30.39691779280004 20.446637036655883 62-63 18.617561024245667 30.05002870499467 30.341420583652145 20.99098968710752 64-65 18.466387906079127 30.483435188163398 29.932293341910583 21.117883563846895 66-67 18.668439850319285 30.122198511876526 29.440369731304784 21.768991906499405 68-69 18.727183251260048 29.482214595642986 29.72537881833767 22.065223334759295 70-71 18.954577322107376 29.10826960308221 29.76561846751526 22.171534607295158 72-73 18.760974574363395 28.42663999536897 30.45078566696468 22.36159976330295 74-75 18.39158657248363 29.311254358538875 29.51213314290861 22.78502592606889 76-77 18.221335607072188 28.59231969626281 30.2968194469023 22.889525249762706 78-79 18.210097455289432 28.5198666282786 30.093402024380712 23.176633892051257 80-81 18.31458234485199 28.242124986931945 29.910654689623563 23.53263797859251 82-83 18.093145502449758 28.353016114036812 30.241565786383656 23.312272597129777 84-85 18.06160882864743 28.10641466466386 29.87468518512562 23.95729132156309 86-87 17.840498650773995 28.410936610499842 29.57233234379372 24.17623239493244 88-89 18.063259870776708 27.94441628245669 29.282352089217177 24.70997175754942 90-91 18.093635856269973 27.494374739645743 29.58460729329681 24.82738211078747 92-93 18.08334405558415 27.629632012352033 29.62107565620175 24.665948275862068 94-95 17.960368648770366 27.306387016872275 29.627812716130798 25.10543161822656 96-97 17.72134591441817 27.41678114252808 29.51530866660018 25.346564276453577 98-99 18.016641140812624 27.211096667910784 28.970771824605208 25.801490366671388 100-101 17.881989675302744 26.869461732683618 29.5944098679661 25.654138724047538 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2371.0 1 1388.0 2 427.0 3 452.5 4 371.5 5 358.0 6 601.5 7 1018.0 8 1296.5 9 1277.0 10 983.5 11 648.0 12 552.5 13 547.5 14 591.5 15 630.5 16 638.5 17 696.5 18 792.5 19 864.0 20 974.0 21 1176.0 22 1405.0 23 1658.5 24 1954.5 25 2332.5 26 2966.0 27 3725.5 28 4248.0 29 4806.5 30 5563.0 31 6209.0 32 6795.5 33 7362.5 34 8424.0 35 9727.5 36 10590.5 37 11142.0 38 10808.0 39 10275.0 40 10403.5 41 10463.5 42 10487.5 43 10755.5 44 10968.0 45 11245.5 46 11550.5 47 12525.0 48 14056.5 49 13784.0 50 11919.5 51 10097.5 52 8412.0 53 7008.0 54 5915.0 55 5021.5 56 4189.0 57 3396.5 58 2791.0 59 2202.5 60 1627.0 61 1173.0 62 844.0 63 604.0 64 421.0 65 328.5 66 256.5 67 201.0 68 164.0 69 131.5 70 105.0 71 83.5 72 73.5 73 64.5 74 51.0 75 47.0 76 39.5 77 27.5 78 22.0 79 14.0 80 6.0 81 3.0 82 1.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.2159924874415493E-4 2 0.044702295575437534 3 0.0 4 0.0 5 0.042772700082972605 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005467187228650634 26-27 0.0019295954924649298 28-29 0.0 30-31 0.0012863969949766197 32-33 4.8239887311623246E-4 34-35 0.009647977462324648 36-37 0.02170794929023046 38-39 0.0038591909849298596 40-41 0.0011255973706045422 42-43 0.0014471966193486973 44-45 0.007396782721115563 46-47 0.0 48-49 0.0 50-51 3.2159924874415493E-4 52-53 0.0 54-55 8.039981218603873E-4 56-57 0.0 58-59 0.020421552295253838 60-61 0.0 62-63 0.007879181594231795 64-65 0.014793565442231127 66-67 0.005627986853022711 68-69 0.014471966193486972 70-71 0.0 72-73 0.0 74-75 0.019939153422137604 76-77 0.0485614865603674 78-79 0.02749673576762525 80-81 0.023637544782695388 82-83 0.031838325625671335 84-85 0.01575836318846359 86-87 0.008683179716092183 88-89 0.07830941706920173 90-91 0.02299434628520708 92-93 0.021225550417114224 94-95 0.02604953914827655 96-97 0.03505431811311289 98-99 0.04823988731162324 100-101 0.013185569198510353 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 310946.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.415623062410205 #Duplication Level Percentage of deduplicated Percentage of total 1 78.1514205893415 46.434153475281974 2 13.221300049118003 15.711035602268417 3 4.166935174099601 7.427431488894015 4 1.8454392415765644 4.385916894483732 5 0.9195898281343653 2.731900130022901 6 0.468020517114258 1.6684638378201035 7 0.2830144126724847 1.1770834365204434 8 0.18455009946722944 0.8772127316860171 9 0.12267498692731846 0.6559929704213686 >10 0.5434557575617749 5.8194078199905555 >50 0.05139848369223832 2.124209720159116 >100 0.03516738357889989 4.139765855963844 >500 0.0032462200226676826 1.4755028171202087 >1k 0.0037872566931122964 5.371923219367279 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3558 1.1442501270317034 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3394 1.0915078502376618 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3255 1.0468055546622244 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2409 0.7747325902246692 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 1672 0.5377139439002271 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1242 0.3994262669402404 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1181 0.37980871276684697 TruSeq Adapter, Index 11 (95% over 21bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 906 0.2913689193622044 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 886 0.2849369343873213 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 870 0.2797913464074148 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 757 0.2434506312993253 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 640 0.20582351919625916 RNA PCR Primer, Index 11 (95% over 23bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 531 0.17076920108314628 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 408 0.1312124934876152 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 382 0.12285091302026718 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 379 0.12188611527403471 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 371 0.11931332128408147 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 356 0.11448933255291917 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 353 0.1135245348066867 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 348 0.11191653856296592 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 340 0.10934374457301267 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 334 0.10741414908054775 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 323 0.10387655734436203 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.6079962437207747E-4 0.0 12-13 0.0 0.0 0.0 3.2159924874415493E-4 0.0 14-15 0.0 0.0 0.0 3.2159924874415493E-4 0.0 16-17 0.0 0.0 0.0 4.823988731162324E-4 0.0 18-19 0.0 0.0 0.0 6.431984974883099E-4 0.0 20-21 0.0 0.0 0.0 0.001447196619348697 0.0 22-23 0.0 0.0 0.0 0.005627986853022711 0.0 24-25 0.0 0.0 0.0 0.013828767695998662 0.0 26-27 0.0 0.0 0.0 0.026853537270136937 0.0 28-29 0.0 0.0 0.0 0.05740546590083166 0.0 30-31 0.0 0.0 0.0 0.1255845066345925 0.0 32-33 0.0 0.0 0.0 0.2397522399387675 0.0 34-35 0.0 0.0 0.0 0.43335498768274877 0.0 36-37 0.0 0.0 0.0 0.7123423359683032 0.0 38-39 0.0 0.0 0.0 1.0302431933519003 0.0 40-41 0.0 0.0 0.0 1.3150193281148495 0.0 42-43 0.0 0.0 0.0 1.6153930264418903 0.0 44-45 0.0 0.0 0.0 1.9931113440919002 0.0 46-47 0.0 0.0 0.0 2.410868768210557 0.0 48-49 0.0 0.0 0.0 2.7972702655766595 0.0 50-51 0.0 0.0 0.0 3.2034501167405276 0.0 52-53 0.0 0.0 0.0 3.5604252828465395 0.0 54-55 0.0 0.0 0.0 3.987509085178777 0.0 56-57 0.0 0.0 0.0 4.493706302702077 0.0 58-59 0.0 0.0 0.0 4.991702739382401 0.0 60-61 0.0 0.0 0.0 5.516070314459745 0.0 62-63 0.0 0.0 0.0 6.000881181941558 0.0 64-65 0.0 0.0 0.0 6.52106796678523 0.0 66-67 0.0 0.0 0.0 7.144005711602658 0.0 68-69 0.0 0.0 0.0 7.82836891293022 0.0 70-71 0.0 0.0 0.0 8.549233628990242 0.0 72-73 0.0 0.0 0.0 9.245978401394455 0.0 74-75 0.0 0.0 0.0 9.973757501302476 0.0 76-77 0.0 0.0 0.0 10.740128511059797 0.0 78-79 0.0 0.0 0.0 11.588989728119994 0.0 80-81 0.0 0.0 0.0 12.478694049770699 0.0 82-83 0.0 0.0 0.0 13.311636104018062 0.0 84-85 0.0 0.0 0.0 14.18011487525165 0.0 86-87 0.0 0.0 0.0 15.07239199089231 0.0 88-89 0.0 0.0 0.0 16.03059695252552 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGTCA 25 0.005022248 56.987354 3 GTCTAAG 75 3.0657218E-4 31.680027 1 CATTCCG 85 6.416953E-4 27.925993 9 GGTATCA 3400 0.0 24.458845 1 TAGAGAG 195 1.564149E-8 24.35357 5 GTATAGA 100 0.0016468909 23.76002 1 ATATAAG 100 0.0016530752 23.74473 3 GAGTACT 2725 0.0 23.257998 12-13 GTATCAA 4430 0.0 22.41916 1 CATGGGT 385 0.0 22.202866 4 GTACTTT 2805 0.0 21.917673 14-15 GTGTGAC 110 0.0028656684 21.60002 1 GAACTGC 55 0.0028822897 21.582647 28-29 AGTACTT 2605 0.0 21.23126 12-13 CTGTATA 180 5.1586594E-6 21.106428 4 CATGGGG 815 0.0 20.97694 4 ATGGGGG 365 0.0 20.817299 5 CTCCGAC 80 4.9683833E-5 20.790018 94-95 TAGGACA 160 4.9827817E-5 20.77664 4 TACATGG 2020 0.0 20.7018 2 >>END_MODULE