FastQCFastQC Report
Thu 26 May 2016
SRR938298_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938298_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317328
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39911.2576892048605859No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36271.1429813946452883No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31881.0046387334240912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24560.7739625876065144No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13240.4172339030908082No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10060.3170221348257954No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8980.2829879493773005No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8380.26408006857258104No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7800.2458024504613523No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5730.18057026168507032No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4740.14937225835728332No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4030.12699793273836535No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3900.12290122523067615No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG3580.1128170221348258No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3560.11218675944133516No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3520.11092623405435387No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC3510.11061110270760854No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3450.10872031462713658No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.10809005193364593No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT3190.10052689961175817No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACA156.5982144E-495.055212
CCCTATG600.003887071331.7652321
TCTTACG300.003949067631.66758588-89
GATCACG300.00395059231.6650992-93
GATAGGT650.00583470229.2339217
GCCTAGG1056.7460314E-527.2273431
GGTATCA35900.026.1465911
GTATCAA47950.023.948141
GAGTACT28950.023.79358512-13
GTACTTT29900.022.64040214-15
GCCGTAT953.2613207E-722.51485394-95
TAGGACA1302.923502E-421.9358164
AGTACTT28050.021.93195512-13
CGCCGTA652.9404878E-421.92198492-93
TATCAAC53800.021.3785882
TACATGG21100.021.1734332
TAGCCCT2257.0671376E-821.1233794
TAGGCAT1805.130458E-621.1200495
GTACATG21500.021.05371
TCAACGC54850.020.9693364
Produced by FastQC (version 0.11.5)